Assistant Injection Molding

PRATHA BUDHANI

Mobile: 817-***-**** Email Id: ******.*******@*****.*** 402 S Cooper Street Apt 115 Arlington Texas 76013, USA

OBJECTIVE: To contribute effectively towards an organization where I can showcase my research skills.

NATIONALITY: USA

EDUCATION:

Master of Science in Bioengineering, University of Texas, Arlington TX [Aug 2011–Dec 2013]

Bachelor of Technology in Biomedical Engineering, VIT University, Vellore, INDIA [May 2007 – May 2011]

WORK EXPERIENCE:

Biology/Biochemistry Research and Development Intern, Life Technologies, Eugene OR, USA [June 2013 Aug 2013]

Project 1: Developing a reagent used as reference to calibrate and align the violet laser source, optics and flow rate of a Flow Cytometer.

● Designed Flow Process Map for protocol in order to determine possible control and noise factors in the experimental procedure.

● Identifying prospective dyes for reagent formulation and conducting spectral measurements to observe absorption and emission spectra.

● Conducting Flow cytometry studies using the Attune® in order to gather necessary data.

● Performed Measurement systems analysis(MSA) in order to test user to user, user to instrument and user to sample variability.

● Full Factorial Design of Experiments(DOE) conducted in order to determine ideal concentration of dye and organic solvent in flow reagent.

● Confocal microscopy to determine whether the reagent is violet laser excitable or not.

● Developed a protocol for full scale production of the reagent.

Project 2: Developing small fluorescent amine functionalized hydrogel beads for cell analysis by flow cytometry.

● Attempts to conjugate secondary antibodies(Goat Anti–Mouse IgG) on the surface of fluorescent dye hydrogels using SMCC conjugation method.

● Obtained a Dye vs intensity curve using Attune® Flow cytometer for different hydrogel samples.

● Performed Immunosorbent Assay(ISA) to determine whether successful secondary antibody conjugation took place.

● Imaging of fluorescently stained hydrogel beads using Upright microscope.

RESEARCH EXPERIENCE:

Graduate Research Assistant, Dept. of Bioengineering, University of Texas at Arlington, USA [Sept 2011 May 2012]

● Synthesized magnetic based core shell nanoparticles for drug delivery application in melanoma skin cancer.

● Characterization of nanoparticles via SEM, DLS and FTIR and LCST measurement.

● Cell culture of Human dermal fibroblasts (HDF) and murine melanoma (B16F10).

Research Assistant, Dept. of Biomedical engineering, Indian Institute of Technology Delhi (IITD), INDIA [Apr 2011 – Aug 2011]

● Processing of novel anti microbial polymer for water disinfection purposes.

● Conducted morphology studies via Scanning Electron Microscopy (SEM).

● Measured kinetics of the polymer over a period of three months in Phosphate buffered solution (PBS).

Research Assistant, Dept. of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur (IITK), INDIA [Feb 2009 Aug 2009]

● Prepared biocomposite cryogel scaffolds and foams for bone tissue engineering purposes.

● Conducted morphology studies via Scanning Electron Microscopy(SEM).

● Performed Mechanical compression studies using INSTRON 1195 and obtained stress vs strain curve.

● Conducted degradation studies over a one month period by suspending in simulated body fluid (SBF).

● Performed density measurements of the scaffold prepared.

● Performed MTT assay using NIH 3T3 fibroblast cells.

TECHNICAL SKILLS:

Languages: C, C++, MATLAB

Software Tools: JMP(DOE and MSA), SAS 9.2, ANSYS, SolidWorks, MULTISIM, VSPICE, ImageJ, Adobe Photoshop, MS Office

Polymer synthesis and processing techniques(injection molding, extrusion), Cell culture and staining techniques, Cell

Techniques: Assays (MTT, BCA, Trypan Blue, Alamar Blue), ELISA, RNA/DNA extraction, Scaffold fabrication techniques,

Hydrogel and Cryogel synthesis, Nanoparticle and microparticle synthesis, Spinal Laminectomy, Brain surgery,

Perfusion.

Flow Cytometer, Microscopy(Confocal, Upright, SEM), DLS(particle sizing and zeta potential analyzer), FTIR,

Equipment: INSTRON, Viscometer, Spectrophotometer, Sonicator, Centrifuge, Multimeter, Soldering, Function Generator,

Oscilloscope, Hemocytometer.

Operating Systems: Windows

PROJECTS:

Synthesis of Bovine serum albumin (BSA) loaded PNIPA PEGDA nanoparticles for wound healing purposes [Spring 2012]

● Three separate batches of PNIPA PEGDA, PEGDA and PNIPA nanoparticles were synthesized.

● Cumulative drug release studies of three batches were done at temperatures above and below lower critical solution temperature (LCST) i.e. at 25

and 37 degrees C.

Synthesis and Mechanical testing studies on PEGMC/methyl diethanolamine (MDEA) hydrogels [Fall 2011]

● Preparation of four different batches of PEGMC hydrogels with varying amounts of methyl diethanolamine (MDEA) crosslinker used.

● Mechanical compression studies of the hydrogels prepared in order to measure modulus of elasticity.

● Nuclear Magnetic Resonance (NMR) and Fourier transform infrared spectroscopy (FTIR) spectra of PEGMC/MDEA.

● Measurement of viscosity of PEGMC/MDEA polymer using both absolute and relative viscometry.

● Measurement of injection pressure and shear thinning studies on the hydrogel prepared.

ACHIEVEMENTS:

● Presented a poster titled ‘Biocomposite Porous Scaffolds for Bone Tissue Engineering Applications’ at the 2012 Materials Research

Society(MRS) Fall meeting held at Boston, Massachusetts, USA

Contact this candidate