Medical Quality Control

Elena A. Betancourt

**** ****** **, *** *****, CA 92154 ( ***************@*******.*** ( (619)

***-****

http://www.linkedin.com/in/elenabetancourt

EDUCATION

University of California of San Diego, January 2011 - September 2013

Degree: B.S. Physiology and Neuroscience

Southwestern College, Chula Vista, California, June 2008 - June 2011

Degree: Associates in Sciences in Transfer Studies and General Biology

RESEARCH EXPERIENCE

Salk Institute, La Jolla, CA November 2013-December 2013

Volunteer in Viral Vector Production/Cell Culture in BSLII+ environment:

Production and purification of viral vectors as a service to internal and

external investigators. Routine cell culture and maintenance of cell lines

and cell stocks.

Perform quality control including infectivity assays with multiple read-

outs such as fluorescence microscopy, FACS and plaque assays (including

immuno-cytochemistry). Molecular Biology & Biochemical Techniques: RNA/DNA

extraction and analysis.

PCR, Q-PCR and qRT-PCR with epMotion, NanoSight, NanoDrop and

ultracentrifuges. Maxi/Mega/Mini Preps in general cloning of DNA in

plasmids. Optimization of protocols for time efficient controls adjusted

with lean manufacturing techniques.

Salk Institute, La Jolla, CA (HHMI) April 2013-October 2013

Research technician/mouse colony manager.?Neurosurgery: In vivo tracer

injections in prefrontal cortex, parafascicular thalamic nucleus and

intermediate gray/white layer superior colliculus in rodents.?Pseudo-typed

rabies and AAV8 injections; SOP for euthanasia, goggling for Green

Fluorescent Protein.?Gel embedding of brain, primary and secondary staining

with antibodies.?Shipping and ordering mouse.?Genotyping, PCR, perfusions,

preparing buffers/solutions.?Managing database spreadsheets.?Assist with

single cell picking.

UCSD Center for Human Development, La Jolla, CA, January 2013 - June 2013

Research Assistant in support of standardized tests for behavior, MRI and

fMRI testing sessions, administration assessments, and assist in laboratory

with task development; IQ testing, data entry, photocopying, filing, and

other duties as assigned. Manage projects for investigating in brain

development and cognitive function in participants as young as 3 years of

age.

Moores UCSD Cancer Center, San Diego, CA, July 2011 - September 2012

Research Supervisor in the NIH-funded Continuing Umbrella for Research

Experience 2012, University of California, San Diego. Electrophoresis gel,

preparing enzymes, dilution of enzymes, centrifuging, homogenizing, run and

read spectrophotometer, denaturing enzymes, micro-pipetting, precipitate

enzyme with different concentration to preserve activity, Michaelis Menten

kinetics experiment and dye columns for purification were some techniques

used in the laboratory.

2012 SACNAS National Conference, Seattle, WA - October 2012

Oral and poster presentation with "Micropurification of Phosphofructokinase-

1 and Enzymatic Inhibition by Ascorbate in Cancer Cell Growth"

2012 ABRCMS Annual Conference, San Jose, CA - November 2012

Presented with co-authorship of "Purification of Rabbit Muscle

Phosphofructokinase-1"

TUTORING EXPERIENCE

Unlimited Learning, San Diego, CA, May 2012 - Present

Teaching science for regular and advanced placement courses with elementary

school, middle school, high school and college students. Concentrated on

areas related to Human Physiology, Physics, Reading Skills, Biology,

Genetics, General Chemistry, Geometry and Organic Chemistry.

SKILLS

Laboratory support: Optimization of protocols, proficient in quality

control and automation of robots.

Recombinant DNA techniques: isolation, digestion of DNA, creating genomic

libraries and cloning genes from Vibrio fischeri, ligation of plasmids into

bacterial DNA, transformation of competent E. Coli DH5 alpha cells with

recombinant DNA plasmids, use of epMotion, confocal microscope, perfusion

pumps, prepare buffers, check pH, regulation of biohazards procedures and

voltage clamp usage.

ORGANIZATIONS

MESA Science Program, Southwestern College, August 2009 - present

Assist in Walk on Water Science Competition, shadow in Scripts Medical

Institution in the ergonomics area, and organized workshops for pre-medical

students.

Phi Theta Kappa, Southwestern College, January 2010 - present

Student honors roll society. Developed skills in communication through the

attendance of meetings and creation of documents as Public Relationship

Secretary in Spring 2010 and selected as a candidate to represent the US in

the China's annual conference in 2013.

AMSA Pre-Medical Organization, Southwestern College, August 2009 - present

Acquired knowledge in volunteering with the medical missionary organization

Healing Hearts Across the Borders assisting medical students and doctors

with free appointments in the city of Tijuana. Selected President for Fall

2010 - Spring 2011

Health and Medical Preparation Program, University of California, San

Diego, February 2010 - present

Organized a field trip to a human cadaver laboratory to witness

dissections. Provide free science tutoring for undergraduate students.

CLINICAL VOLUNTEER EXPERIENCE

UCSD Hillcrest Medical Center, May 2010 - July 2013

Supported department staff by performing tasks such as running errands,

transporting equipment and medical records, providing patient transport

assistance, cleaning and changing beds, stocking supplies, and providing

telephone and administrative assistance in Emergency Department

HONORS AND AWARDS

Student of Distinction Award, May 2011

Higher Scholastic Scholarship, May 2011

International Baccalaureate Certificate, May 2008

TECHNICAL SKILLS

Computer: Mac OS, Windows (XP, Vista, 7), Microsoft Office Excel, Word,

Publisher and PowerPoint

Languages: Spanish/English (Proficient with speaking, writing, and

reading)

CERTIFICATIONS

Biohazard Containment and Safety (AAV8 and Rabies Injection)

Salk Institute, October 2013

American Association for Laboratory Animal Science

Salk Institute License 2681488 April 2013

REFERENCES

Lawrence Alfred, PhD

Co-Director of the Creating Scientists for Addressing Cancer Disparities

Program

*******@****.***, 858-***-****

Dr. Raga Bakhiet, PhD.

Director Mathematics, Engineering, Science Achievement Program (MESA)

********@*****.***, 858-***-****, 619-***-****

Percy Russell, PhD

Laboratory Director

********@****.***, 858-***-****, 858-***-****

Georgia Robins Sadler, BSN, MBA, PhD

Director of the Creating Scientists for Addressing Cancer Disparities

Program

*******@****.***, 858-***-****

Terry Sejnowski, BS, PhD

Director of the Computational Neurobiology Department

Principal Scientist contributor to Obama's BRAIN Initiative Project

*****@****.***, 858-***-****

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