Research Associate, Scientist, Post doc jobs

Suganthalakshmi Balasubbu, PhD **** Owens Dr #***, Pleasanton, CA - 94588

(Immigration status: Green card holder) ********.*********@*****.***, 714-***-****

Research Experience

Research Volunteer: UCSF (Jan 2013-present)

Dr. McNamara laboratory, Department of Francis I Proctor Foundation, UCSF.

Project: Autoimmune model of dry eye disease

Description: Immunohistochemistry analysis and studying Gene expression profile of

Glyco-gene chip array in control, AIRE knockout an d AIRE/IL1 double knockout mice,

Sectioning of OCT eye tissues, Cytospin, Western blot and genotyping of mice tail

lysates.

Predoctoral Fellow: AMRF (Jan–Nov 2010)

Department of Genetics, Aravind Medical Research Foundation, Madurai.

Project: Association studies on Glaucoma

Description: Case-control analysis was performed to investigate the association of 5

SNPs on chromosome 2p using 220 unrelated POAG cases and 220 age-matched

unaffected controls. Among the five SNPs screened, SNPs rs10202118 and rs11125375

showed significant association with POAG. This was the first study to demonstrate the

association of SNPs on chromosome 2p in patients with POAG in the Indian population.

The results were published in IOVS 2012.

Predoctoral Fellow: NEI/NIH (2007 – 2008)

Dr. Fielding Hejtmancik laboratory, Ophthalmic Genetics NEI/NIH, Bethesda, MD, USA.

Project: Association studies on diabetic retinopath y(DR) and Linkage analysis of cataract

Description: Investigated the association of 23 polymorphisms in 12 candidate genes,

using more than 700 DNA samples of DR and DNR by DNA sequencing and different

genotyping techniques. Among these, one SNP rs2070600 in RAGE showed statistically

significant association with DR. The results were published in BMC Medical Genetics

2010. Performed linkage analysis of cataract using panel of microsatellite markers. LOD

score > 2.5 – 3 was used to identify the linkage between chromosome loci and the

disease. The markers in chromosome 1 and 3 were showing > 2 LOD score.

Senior Research Fellow: CSIR, AMRF, Madurai (2008 – 2009)

Project: Association studies on diabetic retinopathy

Description: Continued the same association studies performed at NIH.

Junior Research Fellow: AMRF, Madurai. (2003 – 2007)

Project: Molecular genetics of DR and Mutation screening of JXLR and FEVR

Description: The objective of this study was to examine the genetic variations of the

VEGF and eNOS gene and assess their possible relationship to DR in 210 type 2 diabetic

patients of DR and DNR. Three SNPs in VEGF, C(-7)T, T(-1498)C, and C(-634)G were

statistically significant in the DR group when compared wi th DNR group. This was the

first study to show the association of VEGF polymorphisms with DR in Indian population.

The results were published in Molecular vision 2006, which got cited by 90. Performed

mutation screening of JXLR and FEVR as well. Published two papers for JXLR in both

Molecular vision 2007 and American Journal of Ophthalmology 2007.

Education

Kamaraj University, Madurai, India 2010

Aravind Medical Research Foundation

Ph.D, Biomedical Sciences.

Thesis: “Molecular Genetics of Diabetic Retinopathy”

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Awards and Achievements

NIH Predoctoral visiting fellowship award by National Institute of Health, Bethesda,

Maryland, USA. (2007)

Awarded Senior Research Fellowship by Council of Scientific and Industrial Research

(CSIR), New Delhi, India. (2007)

Received a Travel Fellowship Grant to attend Asia -ARVO, International Meeting on

Research in Vision and Ophthalmology in Hyderabad International Convention Center,

Hyderabad, India. (2009)

Awarded Travel Fellowship Grant to attend 18th Annual Indian Eye Research Group

meeting, L.V. Prasad Eye Institute, Hyderabad, India. (2010)

Skills and Techniques

Molecular Biology and Genetic Techniques

DNA isolation from human blood, animal tissues (mouse and rat) and Plasmid,Isolation of RNA

Polymerase Chain Reaction (PCR), RT-PCR

Agarose/Formaldehyde gel electrophoresis

DNA Sequencing by ABI3100, ABI 3130 genetic analyser, TaqMan SNP Genotyping assay using

7900HT fast Real-time PCR, SNaP shot analysis

Single Strand Confirmation Polymorphism (SSCP) analysis and Restriction Fragment Length

Polymorphism (RFLP) analysis

Linkage analysis, Statistical analysis using Exemplar program and R programming language

cDNA library construction

CFG microarray data analysis. Mouse weaning, tail clipping, tail digestion and purification

Experience in online bioinformatics tools

Immunology Techniques

Immunohistochemistry, Different types of staining such as H&E, CD4, PANCK, PAS, β3 tubulin

& CD45 and WGA Staining etc.

Sectioning of OCT tissue,Cytospin

SDS Polyacrylamide Gel Electrophoresis and Western blot

Double immuno and Radial immuno diffusion assay, Immuno dot blot analysis, Elisa Technique

Cell culture Techniques:

Basic cell culture techniques such as subculture, cell counting, freeze downs

Transformation, IPTG induction, competent cell preparation etc.

Scientific Presentations

1. Association of SNPs on chromosome 2p with POAG in a South Indian cohort. ARVO annual

meeting, Fort Lauderdale, Florida, USA. 2011 [Poster]

2. AMD genes and its association with diabetic retinopathy. 18th Annual Meeting of the Indian

Eye Research Group, L.V. Prasad Eye Institute, Hyderabad, India. 2010 [Oral]

3. Lack of EPO gene promoter SNP rs1617640 and Association of RAGE gene exon 3 SNP

rs2070600 with type 2 diabetic retinopathy in South Indian population.

International meeting on Research in Vision and Ophthalmology at Hyderabad International

Convention Center, India. Asia-ARVO, 2009. [Poster]

4. Association studies on diabetic retinopathy.

NEI Focus on Fellows, Twinbrook campus, National Institute of Health, Bethesda, Maryland, USA.

2008 [Oral]

5. Role of VEGF, eNOS and ALR gene polymorphisms in diabetic retinopathy.

Indo-Swedish Symposium on Genomics and Proteomics of Diabetes, Madras Diabetes Research

Foundation, Chennai, India. 2006 [Poster]

6. Is Aldose Reductase Promoter Polymorphism strongly associated with diabetic

retinopathy?

International Symposium on Human origins & Genetics, NCBS, Bangalore, India. 2005 [Poster]

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Symposiums and Conferences attended

ARVO at Fort Lauderdale, Florida, USA. April 27-May 1, 2008.

15th Annual Indian Eye Research Group meeting, L.V.Prasad Eye Institute, Hyderabad,

India. July 29 – 30, 2006.

13th Annual Indian Eye Research Group meeting, Sankara Nethralaya, Vision Research

foundation, Chennai, India. August 20-22, 2004.

Aqua-Terr annual symposium held in Madurai Kamaraj University on 28 th February 2003.

National symposium on Functional Genomics held in Madurai Kamaraj University, Tamil

Nadu, India. February 15-17, 2002.

Publications

1. “Evaluation of SNPs on chromosome 2p with Primary Open- Angle Glaucoma in

the South Indian Cohort”

Suganthalakshmi Balasubbu,Subbaiah R Krishnadas, Xiaodong Jiao,J.Fielding

Hejtmancik, Periasamy Sundaresan.Invest Ophthalmol Vis Sci.2012 Apr 6;53(4):1861-

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2. “Association Analysis of Nine Candidate Gene Polymorphisms in Indian

Patients with type 2 Diabetic Retinopathy”

Balasubbu Suganthalakshmi, Sundaresan Periasamy, Rajendran Anand, Ramasamy

Kim, Govindarajan Gowthaman, Perumalsamy Namperumalsamy, Hejtmancik James

Fielding. BMC Med Genet. 2010 Nov 10;11:158. Cited by 18.

3. “Unusual manifestations of X-linked retinoschisis: clinical profile and diagnostic

evaluation”

Shukla D, Rajendran A, Gibbs D, Suganthalakshmi B, Zhang K, Sundaresan P.Am J

Ophthalmol. 2007 Sep; 144(3):419-423. Epub 2007 Jul 12. Cited by 14.

4. “Genetic variations in the hotspot region of RS1 gene in Indian patients with

Juvenile X-Linked Retinoschisis”

Balasubbu Suganthalakshmi, Dhananjay Shukla, Anand Rajendran, Ramasamy Kim,

Jayabalan Nallathambi, Periasamy Sundaresan. Molecular Vis. 2007 Apr 19; 13:611-617.

5. “Emerging Patterns of Possible Potential Candidate gene Polymorphisms

associated with Diabetic Retinopathy-a review”

Suganthalakshmi Balasubbu, Anand Rajendran, Kim Ramasamy, Perumalsamy

Namperumalsamy, Periasamy Sundaresan. Asian. J. Exp. Sci., Vol. 20. Supplement,

2006, 15-28.

6. “Association of VEGF and eNOSgene polymorphism in type 2 diabetic

retinopathy”

Balasubbu Suganthalakshmi, Periasamy Sundaresan, Rajendran Anand, Ramasamy

Kim, Rajendran Mahalakshmi, Sundaramoorthy Karthikprakash, Perumalsamy

Namperumalsamy. Molecular Vision 2006; 12:336-341. Cited by 90.

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References

Tom Lietman, M.D.

Dr. Nancy McNamara,

Pearl and Samuel Kimura Professor of Ophthalmology

Associate Professor

Professor of Epidemiology and Biostatistics

UCSF Chancellor/EVC/FAS

Director, Proctor International Center

Proctor Foundation

Associate Director, F.I.Proctor Foundation

513 Parnassus Ave, Med Sciences

Medical Sciences, Room S309

San Francisco CA 94143

University of California San Francisco, 94143-0412

Work phone: 415-***-****

Work email: *****.********@****.***

Dr. J. Fielding Hejtmancik Dr. VR. Muthukkaruppan

Ophthalmic Genetics and Visual Function Branch Director-Research

National Eye Institute, NIH Dr. G. Venkatasamy Eye Research Institute

5635 Fisher’s Lane, Rockville, MD-20852, USA. AMRF, Aravind Eye Hospital

Work phone: +1-301-***-**** #1, Anna Nagar, Madurai, TN-625020, India.

Work email: ***@*****.***.*** Work Phone: +91.452-***-****, Ext.407

Work Email: *****@*******.***

Dr. P. Sundaresan

Senior scientist, Department of Genetics

Dr. G. Venkatasamy Eye Research Institute

AMRF, Aravind Eye Hospital

#1, Anna Nagar, Madurai, TN-625020, India.

Work Phone: +91.452-***-****, Ext.423

Work Email: ******@*******.***

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