Assistant Mechanical

JÉRÔME HOLLENSTEIN, Ph.D.

La Jolla, CA, ***** • 619-***-**** • ******.***********@*****.***

Objective

To gain a full-time position utilizing innovative and technical skills in biotechnology, bioengineering and/or

medical device industry.

Education

Ph.D. in Bioengineering 2009-2013

Swiss Federal Institute of Technology, Lausanne, Switzerland (EPFL)

Collaboration with the University of California, San Diego (UCSD)

M.S. in Bioengineering and Biotechnology 2007-2009

Swiss Federal Institute of Technology, Lausanne, Switzerland (EPFL). GPA 5.51/6

B.S. in Life Sciences and Technology 2004-2007

Swiss Federal Institute of Technology, Lausanne, Switzerland (EPFL). GPA 5.37/6

(Ranked 6 out of 61 graduates)

Experience

Graduate Research Assistant, Cartilage Tissue Engineering (CTE) Lab, UCSD 2009-2013

• Completed Ph.D. work under the supervision of Dr. Robert L. Sah (Co-PI) and Dr. Jennifer H. Chen

• Developed a hydrogel-based diffusion system for osteochondral interface application

• Developed an innovative bioreactor diffusion system

• Created viable cartilage-bone construct with strong interface properties

• Evaluated the mechanical and biological properties of the formed osteochondral construct

• Developed chemical and biological assays

• Trained and mentored students in conducting lab research

• Wrote standard operating procedures for experiments

Graduate Research Assistant, Laboratory of Biomechanics Orthopedics (LBO), EPFL 2009-2013

• Completed Ph.D. work under the supervision of Dr. Dominique D. Pioletti (Co-PI)

• Developed Finite Element Model to test in-vivo behavior of osteochondral construct used in defect repairs

• Validated materials property using a 3D model of mechanical testing reproducing the experimental data

• Developed strong communication skills by collaborating with two professors

• Developed critical thinking and troubleshooting skills

Teaching Assistant, EPFL 2009-2011

• Teaching assistant for Genetics undergraduate course in Bioengineering Department

• Teaching assistant for Biomolecular Structure and Mechanics graduate course

• Created homework and examinations.

• Time-managed between doctoral research and office hours.

Graduate Research Assistant, CTE Lab, UCSD 2008-2009

• Completed M.S. worked under the supervision of Dr. Robert L. Sah (PI) and Dr. Jennifer H. Chen

• Developed MATLAB code with experimental manipulations to calculate ion diffusivity into hydrogel

• Created hydrogel-bone osteochondral construct and mechanically tested its interface properties

• Hands-on application on laboratory works and problem solving skills

• Learned valuable techniques to create efficient experiments and improve experimental results

Undergraduate Research Assistant, EPFL 2007

• Worked under the supervision of Prof. Jeffrey A. Hubbell.

• Developed test product for “Non-viral Gene Delivery Materials and Methods in Clinical Application”

• Created market analysis, literature review, new product, pre-clinical and clinical cost and duration, and

provided a company budget for the drug production

Relevant Skills

• 3D design (SolidWorks) Histology and microscope imaging

•

• Basic Programming (C, C++, Java, Perl, Hydrogel and osteochondral construct

•

LabView, MATLAB, and ImageJ) manipulation

• Chemical and DNA assays Mammalian and human cell culture

•

• Chromatography and electrophoresis Mass transport and diffusion

•

• Finite Element Method (COMSOL)

Mechanical testing of synthetic and bio- Statistical analysis

• •

logical materials Technical writing & communication such

•

Micro-computed tomography and associ- as full-length publications, conference

•

ated image processing abstracts & presentations, and SOPs

Scanning electron microscopy and X-ray Tissue engineering

• •

diffraction (Basic use)

Communication and Leadership

• Mentored three master students and three undergraduate students while conducting Ph.D.

• One journal publication, three publications (submissions in progress), two podium presentations and four

poster presentations at scientific research conferences.

• Taught annual internal summer seminar entitled “Multi-Scale Structure & Composition of Cartilage”.

• Co-founder and organizer of an open-air music festival (www.grouna.com). Founded the festival in 2003,

which was held annually in September in Geneva until 2011.

Additional Languages

• French (fluent, native language)

• German (conversational)

Awards

AO Foundation Grant (S-10-10P) 2010-2012

•

Mention of Excellence, Master Project, EPFL 2009

•

Dean’s List, Bachelor of Science, EPFL 2007

•

Publications and Presentations

• Hollenstein J, Unger JA, Lucas TM, Jann V, Chung AM, Wong VW, Cory E, Pioletti DP, Sah RL: Crea-

tion of an Osteochondral Graft by Rapid Double-Diffusion Formation of a Calcified Layer that Attaches

Articular Cartilage to Trabecular Bone. Trans Orthop Res Soc 39:219, 2014.

• Hollenstein J, Terrier A, Cory E, Chen AC, Sah RL, Pioletti DP: Mechanical Evaluation of an

Engineered Zone of Calcification in a Bone-Hydrogel Osteochondral Construct. Published in Computer

Methods in Biomechanical and Biomedical Engineering, 2013 [Epub ahead of print].

• Hollenstein J, Terrier A, Cory E, Chen AC, Sah RL, Pioletti DP: Mechanical evaluation of an engineered

zone of calcification at the interface between bone and hydrogel. Trans Orthop Res Soc 38:530, 2013.

• Hollenstein J, Chen AC, Terrier A, Sah RL, Pioletti DP: Parametric Analysis of an Engineered Zone of

Calcification at the Interface Between Bone and Hydrogel: Mechanical and Numerical Evaluation.

CMBBE, 2012.

• Abada EN, Hollenstein J, Sah RL: Integration of articular cartilage and trabecular bone through the for-

mation of a calcified zone. UCSD Summer Res Conf, 2012.

• Hollenstein J, Hwang J, Cory E, Pioletti DP, Sah RL: Diffusion Limited Process to Engineer a Localized

and Stiff Calcification Zone. AOER Symposium, 2011.

• Hollenstein J, Hwang J, Cory E, Pioletti DP, Sah RL: Engineering a zone of calcification that is localized

in space and mechanically stiff. Trans Orthop Res Soc 36:1895, 2011.

Relevant Completed Courses

• Advanced Transport Phenomena Immunology and Microbiology

•

• Artificial Organs Management of Technology and Entre-

•

preneurship

• Bioinformatics (I, II, III)

Mass Transfer

• Biomechanics •

Materials Science

• Biomechanics, Biofluids and Biotransport •

Neurophsiology

• Biomicroscopy •

Numerical Analysis

• Biomolecular Structure and Mechanics •

Pharmacology and Pharmacokinetics

• Biooriented chemistry •

Physiology

• Developmental Biology •

Polymer Science

• Drug Discovery •

Probability and Statistics

• Genetics •

Signals and Systems

• GLP/GMP •

Stem Cell Biology and Technology

• Image Processing •

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