Technician Objective

Location:

Posted:

December 04, 2013

Matthew N. Ieraci

Pittsburgh, PA 15227

*******@*****.***

Objective

A position as a Histology Technician at Allegheny Valley Hospital.

Education

Pennsylvania State University

• B.A., Biology, December 2011

• Major GPA: 3.66

• ASCP Histotechnologist certification willing and eligible within 6 months of hire.

Experience

Histology Technician

University of Pittsburgh Adipose Stem Cell Center June – August 2010, 2011

University of Pittsburgh Department of Medicine - Vascular Medicine Institute January-October 2013

Skills

Histology

• Tissue preparation and experience

- Excision of sample from model organism.

- Tissue fixation and dehydration.

- Tissue infiltration and embedding using freezing medium, paraffin or JB-4 resin.

- Human Adipose, skin, muscle, vasculature, zebrafish brain, eye, heart and spinal cord.

• Sectioning

- Frozen

- Paraffin

- Plastic (JB-4 resin)

• Basic staining

- H&E, toluidine blue, Masson’s trichrome, AFOG, DAPI etc.

• Immunohistochemistry

- Experience with monoclonal and polyclonal antibodies.

- PCNA, MEF2C, CD-31, Hgb, Ngb, CYgb-1, CYgb-2, androgens and androgen receptors.

- Experience with protocol creation, optimization and troubleshooting.

Publications

Lin, Y.-C.; Grahovac, T.L.; Oh, S.J.; Ieraci, M.N.; Rubin, J.P.; Marra, K.G.* “Evaluation of a Multi-

layer Adipose-Derived Stem Cell Sheet in a Full-thickness Wound Healing Model,” Acta Biomater.