Molecular Biology, Biochemistry & Microbiology experienced researcher

Priyasri Shotton (Chaurasia) Ph.D.

Phone: +1-317-***-****

Email: acb982@r.postjobfree.com

Skype ID: priya.shotton

USA Green Card / Indian Passport

Six years of Ph.D. research experience in yeast gene regulation and its

synchrony with DNA repair. Expert in molecular biology & biochemistry

techniques with knowledge of research design, data analysis & synthesis.

Currently working as R&D head to establish a biotech lab in Riyadh, KSA.

Well-rounded & highly analytical individual with both research &

administration proficiency.

SUMMARY

My last job involved setting up a production lab for Taq polymerase & other

research reagents in Riyadh, Saudi Arabia with the objective to supply to

the GCC countries. Prior to this I acquired my Ph.D. at Southern Illinois

University, Carbondale. I worked on yeast gene regulation & DNA repair. I

gained strong expertise in Protein Biochemistry -expression, protein

purification & production of recombinant proteins, Chromatin

Immunoprecipitation, Nucleic acid isolation & purification, PCR, Western

blotting, Protein tagging, Transformation, Northern blotting, Gel

electrophoresis, Cloning, Yeast growth, Genetic screening & analysis, In

vivo & In vitro transcription, Spectrophotometry, Primer extension, Basic

bioinformatics, RT-PCR, Microbiological techniques & working in aseptic

environments. I am target oriented, result driven, well organized and

competent. I am able to multi task & lead projects to meet dead lines. I am

also a team player with valuable interpersonal and communication skills.

The evidence of this is my many publications in peer-reviewed international

journals. I am a quick, enthusiastic learner & motivated to transfer my

bench proficiency and experience to match the job expectations. I am also

an invited reviewer for the journal "Gene", Elsevier publication group.

WORK & RESEARCH EXPERIENCE

Lab Consultant & Chief Lab Specialist

Biomedical Reagent Factory Riyadh, KSA. June 2013 - Jan 2014

This lab is currently being setup to supply Taq Polymerase to the GCC

countries. I am in charge of the lab R&D- my duties include but are not

limited to: . lab designing and planning . protocol finalization .

validation studies . development of standard operating procedures .

procurement of reagents. I am also responsible for . staff hiring to build

the team . obtaining quotations from vendors for lab furniture, instruments

and reagents. I am the scientist that responds to the CEO.

Ph.D. Candidate & Graduate Research Assistant

Southern Illinois University Carbondale, USA. 2007-2012

Thesis Title: "A STUDY OF THE INTERPLAY BETWEEN SACCHAROMYCES CEREVISIAE

TRANSCRIPTION AND DNA REPAIR".

My responsibilities included but were not limited to . Project & Time

Management . Team Work . Workflow Administration . Project initiation &

leadership . Laboratory Procedures . Solution & Media Preparation . Cell

Generation . DNA & RNA purification . Molecular Biology & Biochemistry

Techniques . Lab record maintenance

I studied gene regulation and its synchrony with DNA repair using yeast as

a model system. I led one of my projects to investigate both single strand

DNA damage as well as double stranded DNA breaks. And my work was able to

demonstrate, for the first time, the preferential repair of double strand

DNA breaks at actively transcribing genes. This data has the potential to

be extended to humans. Additionally, I have managed several team projects

and published them.

I was also a Student Mentor and . Developed and executed rotation projects

for graduate students in their first few years. . Organized and provided

scientific, technical training to Middle School and High School summer

trainees:

V -Trained Illinois State fair "Illinois Junior Academy of Science" 2009

Gold Medal winner; Srinivas Panchmukhi.

V -Trained 2010 American Cancer Society Summer Research Fellow; Luke

Stegmann.

In 2011 one of my papers was selected for Faculty of 1000- Biology which

recognizes the top 2% of scientific contribution in the field of biological

science. "Rad26p regulates the occupancy of histone H2A-H2B dimer at the

active genes in vivo". Chaurasia P, Malik S, Lahudkar S, Uprety B, Bhaumik

SR. Nucleic Acids Res. 2011 Dec.

Presented work at professional conferences including one at "Mechanism of

Eukaryotic Transcription" Cold Spring Harbor Lab, New York. Additionally, I

was invited as a judge for Science Fairs held at SIUC in 2011 and 2012.

Graduate Research Assistant

Southern Illinois University Carbondale, USA. 2005-2007

I worked on Archaeal gene regulation by studying transcription and post-

transcriptional modification of t-RNAs in halophilic & thermophilic

archaea. The work involved . RNA transcription . lead acetate based Foot

Printing . Northern Blot . Thin Layer Chromatography and more.

Masters Trainee

National Institute of Virology; Pune, India. 2004

Masters Thesis Title: Monoclonal Antibody production of West Nile Virus.

The work involved . basic virological techniques . complementation assays .

mouse cell culture . ELISA . immunoflourescence . virus neutralization

tests . monoclonal antibody production and more.

Volunteer Teacher

Apeejay School; Kolkata, India. 2012-2013

I spent time at my alma mater to assist students in their biology course

work. This helped me with my . interpersonal skills . presentation and .

organizational skills.

ACADEMIC PRESENTATIONS

. Poster presentation at Cold Spring Harbor Lab, New York - Mechanisms

of Eukaryotic Transcription 2011.

. Poster presentation at Research Town meeting, SIUC 2012.

PEER REVIEWED PUBLICATIONS

. Functional Analysis of Rad14p, a DNA Damage Recognition Factor in

Nucleotide Excision Repair, in Regulation of Transcription in vivo.

Chaurasia P, Sen R, Bhaumik SR. J Biol Chem. 2012 Nov.

. Preferential repair of DNA double-strand break at the active gene in

vivo. Chaurasia P, Sen R, Pandita TK, Bhaumik SR. J Biol Chem. 2012

Aug.

. Rad26p regulates the occupancy of histone H2A-H2B dimer at the active

genes in vivo. Chaurasia P, Malik S, Lahudkar S, Uprety B, Bhaumik SR.

Nucleic Acids Res. 2011 Dec.

. Regulation of chromatin assembly/disassembly by Rtt109p, a histone H3

Lys56-specific acetyltransferase, in vivo. Chaurasia P, Durairaj G,

Lahudkar S, Malik S, Shukla A, Bhaumik SR. J Biol Chem. 2010 Oct.

. Rad26p, a transcription-coupled repair factor, is recruited to the

site of DNA lesion in an elongating RNA polymerase II-dependent manner

in vivo. Chaurasia P, Malik S, Lahudkar S, Durairaj G, Shukla A,

Bhaumik SR. Nucleic Acids Res. 2010 Mar.

. Histone methylation and ubiquitination with their cross-talk and roles

in gene expression and stability. Shukla A, Chaurasia P, Bhaumik SR.

Cell Mol Life Sci. 2009. Review.

. Elongating RNA polymerase II is disassembled through specific

degradation of its largest but not other subunits in response to DNA

damage in vivo. Chaurasia P,Malik S, Bagla S, Duan Z, Bhaumik SR. J

Biol Chem. 2008 Mar.

. Differential roles of archaeal box H/ACA proteins in guide RNA-

dependent and independent pseudouridine formation.

Gurha P, Joardar A, Chaurasia P, Gupta R. RNA Biol. 2007 Oct.

. Wnt pathway and breast cancer. Mohinta S, Wu H, Chaurasia P, Watabe K.

Front Biosci. 2007 May. Review.

. DNA damage and repair: A review. Chaurasia P and Bhaumik S.R.

(manuscript under preparation)

EDUCATION

Ph.D. - Biochemistry & Molecular Biology; 2007- 2012

Southern Illinois University Carbondale, USA

Master of Science - Biotechnology; 2002- 2004

Jiwaji University, Gwalior, India - 1st Division

Bachelor of Science - Biotechnology; 1999 - 2002

Nagpur University, Nagpur, India - 1st Division

TRAININGS & CERTIFICATION

. Honors diploma in Web-centric computing; Academic Council of NIIT,

Nagpur. 2002

. Summer Trainee- Bsc. Biotechnology

Central Institute of Cotton Research, Nagpur. 2001

. Summer Trainee- Bsc. Biotechnology

Orange City Hospital Research Institute Dept of Pathology, Nagpur.

2000

A+ grade.

SKILLS

Software:

. MS Office

. EndNote

. Zotero

. Canvas

. Adobe Illustrator

. Internet Research

. Self-taught hardware and software problem solver for Windows

Languages:

. English

. Hindi

. Bengali

. Gujarati

Contact this candidate