Sandeep Putty

**** ***** ******, *** ** Email: acb8pl@r.postjobfree.com

Kansas City, MO 64112 Phone: 816-***-****

Qualification Summary

Development and validation of analytical methods as per FDA and ICH guidelines, for quantifying small

organic molecules and peptides using HPLC, LC-MS/MS and GC-MS

Strong scientific skills in biological sample preparation (protein precipitation, liquid-liquid and solid phase

extraction) of plasma and various tissues, analytical method development, validation, chromatographic

separations, data acquisition, data analysis and data interpretation

Troubleshoot analytical methods and laboratory instruments

Quality control analysis on raw material, intermediate and finished products using various analytical

instruments (HPLC, GC, UV-Vis, FT-IR, and KF) in a cGLP and cGMP environment

Structure elucidation of bio-organic molecules and degradation products using FT-IR, NMR (proton and

carbon) and LC-MS

Purification of bio-organic molecules and peptides using silica column chromatography, preparative HPLC

and distillation (normal and vacuum)

Maintain appropriate documentation (records and lab notebook)

Review of scientific literature, write research articles, grants and standard operating procedures (SOPs)

Creative, confident, positive and can-do attitude with excellent communication and presentation skills

Software: LIMS, SPSS, ChemDraw, Endnote, Microsoft Office Suite, Analyst, Chemstation, MOE

(Molecular Operating Environment), Chimera

Education

Ph.D. Candidate in Pharmaceutical Sciences & Pharmacology GPA: 3.76

University of Missouri-Kansas City Aug 2005 - Present

Dissertation Research: 1. Characterization of D-boroAla as a novel broad spectrum antibacterial agent and

confirmation of D-Ala-D-Ala ligase as the target in Escherichia coli using LC-MS/MS. 2. Modeling

reactivity and linear free energy relationships in the design of solid-phase linkers.

Master of Science in Chemistry GPA: 4.0

Western Illinois University 2003 - 2005

Thesis Title: Synthesis and characterization of zinc (II) iodide- phenothiazine complexes

Bachelor of Pharmacy (Honors) GPA: 7.33/10

1999 – 2003

Birla Institute of Technology & Science (BITS), Pilani, India.

Teaching Experience

Curator for Mass Spectrometer (ABI QTRAP 2000 and 3200) 2006 - Present

Responsible for periodic maintenance, identify any problems with LC-MS, troubleshoot the problems, tune

and calibrate Mass Spec, and train new students on using and handling of LC-MS.

Labs: In charge of setting up the lab, explain theory and principle of the experiment, demonstrate how to

handle instruments, prepare reagents for the experiment, help students in performing the experiment,

evaluate theory based quiz and experimental results.

Courses: Conduct problem solving and doubt clarification sessions, proctor and grade exams.

Industrial Experience

Jan 03 – Jun 03

Granules India Limited (FDA approved 100% export oriented company)

Analyzed raw materials for bulk drugs, quality control samples of in-process and finished goods using

several analytical instruments (HPLC, GC, UV-Vis, FTIR, KF) in accordance with USP/EP and conducted

stability studies on finished products as per ICH guidelines in a cGLP and cGMP environment.

Page 1 of 3

Sandeep Putty

Research Experience

Synthesized D-boroAlanine and its analogues; characterized these molecules using GC-MS and NMR

(proton and carbon); developed a LC-MS/MS (MRM mode) assay and determined the in vivo biochemical

mechanism of D-boroAlanine in E.Coli and S.aereus.

Examined in vivo effects of quercetin on 17β-estradiol-induced breast cancer in rats, following the

ingestion of quercetin enriched diet. A LC-MS/MS method was developed to determine total quercetin

(parent quercetin and the quercetin after enzymatic hydrolysis with β-glucuronidase/sulfatase) in rat serum

samples.

Studied the effect of concentration of vancomycin on the growth of VRE (vancomycin resistant

enterococci) by analyzing the metabolites in vivo. A LC-MS/MS assay for marfey's derivatives of L-Ala,

D-Ala, D-Ala-D-Ala and D-Ala-D-Lac (Ala:Alanine, Lac:Lactate) was developed to quantitate the effect of

vancomycin on these metabolites.

Purified crocetin from saffron by preparative HPLC and characterized by LC-MS and NMR.

Synthesized cyclic GlyGlu analogues; characterized these molecules using LC-MS, GC-MS and NMR; A

LC-MS/MS method was developed to study the degradation kinetics of these analogues in rat serum.

Performed stress degradation (acid hydrolysis, base hydrolysis, various pH, oxidation, temperature and

photolytic) studies on tenofovir drug and identified the degradation products. Stability-indicating LC-MS

method was developed and validated as per ICH guidelines to analyze these stress degradation samples.

Publications

Putty S, Vemula H, Bobba S, Gutheil WG, An LC-MS/MS assay for D-Ala-D-Lac: A key intermediate

for vancomycin resistance in vancomycin resistant enterococci (VRE). Analytical biochemistry. 2013 Nov

15;442(2):166-71.

Putty S, Pagano P, Jamindar D, Quinn DL, Schweizer HP, Gutheil WG, Characterization of D -boroAla as

a novel broad spectrum antibacterial agent targeting D-Ala-D-Ala ligase. Chem Biol Drug Des. 2011 Nov

11;78(5):757-63.

Singh B, Mense SM, Bhat NK, Putty S, Guthiel WG, Remotti F, Bhat HK. Dietary quercetin exacerbates

the development of estrogen-induced breast tumors in female ACI rats. Toxicol Appl Pharmacol. 2010 Sep

1;247(2):83-90.

Ponnaluri VK, Vavilala DT, Putty S, Gutheil WG, Mukherji M, Identification of non-histone substrates

for JMJD2A-C histone demethylases. Biochem Biophys Res Commun. 2009 Dec 11;390(2):280-4.

Vemula H, Bobba S, Putty S, Barbara J, Gutheil WG, LC-MS/MS-Based Quantitation of UDP-Linked

intermediates in the S. aureus cell wall biosynthesis pathway. (Submitted to Anal. Biochem.)

Agrahari V, Putty S, Gutheil WG, Youan BB, Evaluation of degradation pathways of tenofovir using a

stability indicating LC-MS assay. (Manuscript in preparation)

Putty S, Gutheil WG, Development of backbone amide linkers suitable for inverse solid -phase peptide

synthesis(ISPPS). (Manuscript in preparation)

Abstracts

Putty S, Bobba S, and Gutheil WG, A liquid chromatography–tandem mass spectrometry assay for the

Marfey’s derivative of D-Ala-D-Lac: Application to vancomycin resistance in VRE; 60th ASMS annual

meeting, May 2012, Vancouver B.C.

Putty S, and Gutheil WG, Development of Backbone Amide Linker suitable for Inverse Solid-Phase

Peptide Synthesis(ISPPS); 236th American Chemical Society (ACS) National Meeting, Aug., 2008,

Philadelphia, PA.

Page 2 of 3

Sandeep Putty

Putty S, Jamindar D, and Gutheil WG, Development of An LC/MS/MS Assay for D-Ala, L-Ala, and D-

Ala-D-Ala; Early Intermediates in Bacterial Cell Wall Biosynthesis; Midwest Regional ACS Meeting,

Nov., 2007, Kansas City, MO.

Putty S, Alapati VK, Kodimela S, Thimmaiah KN, and Made Gowda NM, Study of Zinc(II)

Complexes of N-alkylphenotiazines; The 16th Annual Illinois Student Research Conference, Apr., 2005,

Chicago, IL.

Alwani V, Putty S, and Made Gowda NM, Synthesis of Mercuric-N-alkylphenothiazine Complexes;

The 36th Annual Great Lakes Regional American Chemical Society Meeting, Oct., 2004, Peoria, IL.

Doddapaneni S, Putty S, Somanagari P, Alwani V, and Made Gowda NM, Synthesis of Palladium(II)-

N-alkylphenothiazine Complexes; The 15th Annual Illinois Student Research Conference, Apr., 2004,

Chicago, IL.

Putty S, Roy A, and Chandran S, Oral controlled delivery of celecoxib- design, evaluation and

optimization; International Symposium on Emerging trends in Genomics and Proteomics, Education

and Research, Jan.,2003, BITS, Pilani, India.

Putty S, Roy A, and Chandran S, Formulation and evaluation of matrix based oral controlled release

tablets of flurbiprofen, nimesulide, diclofenac sodium and celecoxib; International Symposium on

Emerging trends in Genomics and Proteomics, Education and Research, Jan., 2003, BITS, Pilani.

Ravi PR, Putty S, Roy A, and Chandran S, Application of optimization technique in the design of oral

controlled release formulations of celecoxib; 54th Indian Pharmaceutical Congress, Dec., 2002, Pune

India.

Core Competencies

HPLC, Preparative HPLC, Chromatography separation (normal phase, reverse phase, ion pair), enantiomers

separation, LC-MS, LC-MS/MS, GC-MS, UV-Visible spectrophotometer, FT-IR, NMR, KF, Dissolution,

Tablet Hardness & Friability, DSC, TGA, Synthesis, Peptide synthesis, Solid phase peptide synthesis,

Spectrofluorometry, TLC, Wet Chemistry, Separation techniques, Lyophilization, Enzyme based assays,

Data acquisition and analysis, MIC(minimum inhibitory concentration), MBC(minimal bactericidal

concentration) determination, Frequency of resistance, Agarose gel electrophoresis.

Awards and Memberships

Best Outgoing Graduate Student Award 2005

Chancellors Non Resident Student Fellowship Award 2006-2012

Student Graduate Association Travel Award

American Association of Pharmaceutical Scientist (AAPS) & American Chemical Society (ACS)

Leadership and Other Experience

Security department coordinator at BITS pilani 2002-2003.

Secretary, Indian student association, WIU, Macomb, 2004-2005.

Treasurer, pharmaceutical sciences graduate students association, UMKC, 2006-2007.

Student senator in student government association (SGA) representing inter disciplinary student council

(IDSC), UMKC, 2007-2009.

IDSC executive board member, UMKC, 2007-2009.

Completed half marathon in 2011 and full marathon in 2012

Page 3 of 3

Contact this candidate