Plant Engineering
Resume:
Kevin John Jose
*************@*****.***
Education
Master of Biotechnology GPA 3.77
The Pennsylvania State University December 2013
Bachelor of Engineering-Biotechnology GPA 3.70
RV College of Engineering June 2011
Technical Skills
Sterile and aseptic techniques with prior industry experience following GMP, GLP guidelines
Fermentation techniques in seed and pilot fermenters
HPLC, FTIR, Downstream techniques involving microfiltration
Media design, preparation and optimization
DNA/RNA isolation, purification, cloning and expression
Gel electrophoresis, Blotting techniques, PCR, immunohistochemistry
Mammalian, microbial and plant tissue culturing
Animal handling in cancer studies
Experience
Penn State University, Department of Veterinary and Biomedical Sciences May 2013- December 2013
Carcinoma studies conducted on human tumorigenic cell lines like LNCaP, HUH7, A549, PC3
Cell lines were treated with TGF-β, TNF-α to undergo EMT (epithelial to mesenchymal transition) by ligand
activation of PPAR-β (peroxisome proliferator activation receptor)
Cells successfully showed the presence of molecular markers in EMT and with ligand activation showed
attenuated levels of pro-tumorigenic markers
Conducted skin cancer studies in mice through UV exposure and evaluated the role of ligand activation of
PPAR-β in skin cancer
BIOCON in the Process Development Plant Unit September2011- March 2012
Performed off-line analysis on seed and pilot Streptomyces and Pichia fermentation, total nitrogen and
phosphate count, HPLC to determine feed and product concentration
Successfully tested the relevance of near infrared spectroscopy in real time fermentation analysis, compared
the off line data with Fourier Transform Infrared Spectroscopy (FTIR) results to check its relevance
Shake flask trials and media preparation, prepared seed culture to determine optimum pH and temperature for
production strain while following GLP
Downstreaming of Pichia broth after fermentation for the isolation of trypsin, the purification steps involved
microfiltration and diafiltration while following cGMP
Additional Research work
Successfully elicited secondary metabolites via the phosphoenol pyruvate pathway with biotic and abiotic
elicitors by growing rice embryogenic stem cells in controlled tissue culture environment
Leadership Qualities
Founding member and Vice Treasurer of the Penn State Biotech Club, the duties involve planning meets and
raising funds for the club
Organized the Biotech fest ‘Helix 09’ during undergraduate years, a department fest in which I had to
supervise the fund raising and manage the fest with other team members
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