Assistant Engineering

Sanam Dharma

*** ******** *****, *** # ****, South San Francisco 94080, # 404-***-****, acb2pt@r.postjobfree.com

OBJECTIVE: Seeking a full-time opportunity to work in a competitive scientific environment in

a Biotech/Pharma industry with the best utilization of my analytical and technical skills.

ACADEMIC QUALIFICATIONS

Masters in Biology Dec 2013

Georgia State University, Atlanta, GA GPA 3.92/4.00

Bachelor of Engineering, Biotechnology May 2010

University of Mumbai, Thadomal Shahani Engineering College, Mumbai, India GPA 3.90/4.00

TECHNICAL SKILLS

• Laboratory skills: SDS-PAGE, Western Blot, ELISA, Affinity Chromatography, PCR

techniques, Gel Electrophoresis, DNA purification, Restriction enzyme

digestion, Molecular Cloning, Protein Expression and Purification,

Cell Culture Techniques, Immunostaining (Immunofluorescence and

Immunohistochemistry), H&E Staining, Fluorescence Microscopy,

HPLC, small animal handling techniques like Oral Feeding, IP and sub

cutaneous injections, Imaging using IVIS Lumina, tissue

cryopreservation and sectioning.

• Equipments: Centrifuge, Autoclave, UV/VIS spectrophotometer, Microplate Reader,

and Hemocytometer

• Software: MS Office, Adobe photoshop, RasMol, SwissPDB Viewer, HTML, C,

C++

RESEARCH EXPERIENCE

Graduate Research Assistant, Advisor Dr. Ritu Aneja, Department of Biology Jan 2013

– Dec 2013 Georgia State University, Atlanta, GA

• Quantitation of accumulation of tumor specific, cytotoxic NIR dyes

Using cell proliferation assay, estimated the cytotoxicity (IC50) of near infrared

(NIR) dyes.

Treated pancreatic cancer cell lines with NIR dyes at cytotoxic concentrations

of these dyes and quantitated their accumulation using fluorescence microscopy.

• Development of centrosomal amplification profiles for tumors.

Devised a novel formula and technique to account for numerical and structural aberrations in

centrosomes of tumors.

• Examined tumor inhibition efficacy of water soluble noscapine analogs in mice models.

IVIS Lumina imaging was used to assess tumor inhibition activity of analogs.

Graduate Research Assistant, Advisor Dr. Yuan Liu, Department of Biology, Aug 2011 –

Dec 2012

Georgia State University, Atlanta, GA

• Purification of Interleukins from transformed bacteria.

Interleukins were purified on a large scale using affinity chromatography. Further, analysis

was carried out using western blot and SDS-PAGE.

• Cloning of human CD47 in expression vectors in E.coli.

Human CD47 was cloned in expression vector and protein was expressed into and purified

from transformed E.coli.

UNDERGRADUATE RESEARCH EXPERIENCE

Sanam Dharma

100 Mclellan Drive, apt # 3091, South San Francisco 94080, # 404-***-****, acb2pt@r.postjobfree.com

Research Assistant Jan 2010- May 2010 Advisor

Dr. Anita Samagod, Bharat Serum and Vaccine, Ltd.,Maharashtra, India

• Purification of Biomolecules (Antibody and Hormone) from recombinant Chinese Hamster Ovarian

(CHO) Cell Lines

Biomolecules from rCHO cell lines were purified using Gel Extraction chromatography.

Confirmed for purity using SDS-PAGE and Western Blot, quantified using HPLC, Biuret and

Modified Folin Lowry tests.

Bioinformatics project Aug 2008 – Dec 2008

Thadomal Shahani Engineering College, Mumbai, India.

• Analyzed a human protein (hemoglobin) in terms of its sequence, structure and similarities

using Bioinformatics tools like BLAST, RasMol, SwissPDB viewer.

Graduate Teaching Assistant, Principles of Biology I, Department of Biology Aug 2012 –

Dec 2013

Georgia State University, Atlanta, GA

• Instructed 24 students on Scientific method and basic techniques of Biology

including Microscopy, Spectrophotometry, Gel Electrophoresis, and Microbiology for 4

semesters

• Tutored students on lab protocols, instructed and ensured proper use of equipment,

helped them with analyses of data obtained after experiments.

• Prepared lecture materials, quizzes, exams and assignments for students and graded

them.

HONORS

• Awarded scholarship of $7000 p.a. with full tuition waiver, Georgia State University Aug

2012- Dec 2013

• Received scholarship of $6000 p.a. with full tuition waiver, Georgia State University

Aug 2011- Jul 2012

PUBLICATIONS

Zhen Bian, YaLan Guo, Youqun Luo, Alexandra Tremblay, Xiugen Zhang, Sanam Dharma, Aarti

Mishra and Yuan Liu (2012). CD47 Deficiency does not impede polymorphonuclear neutrophil

transmigration but attenuates granulopoiesis at the post-acute stage of colitis. J Immunol.190 (1): 411-

417.

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