IRIS FONTAINE GLOVER

*** ********** **** ***** **********, PA 19014 Phone: 484-***-**** acb1y0@r.postjobfree.com

ASSOCIATE SCIENTIST/RESEARCH TECHNICIAN

Versatile, qualified and highly skilled research professional with an extensive background in a variety of lab

settings, including the application of scientific techniques to a variety of microbiological tests and

examinations in fields such as bacteriology, mycology, virology, serology or parasitology.

Developed, validated, and implemented plans, methods, standard operating procedures, and quality control

and quality assurance procedures.

STANDARD MOLECULAR BIOLOGY TECHNIQUES: purification of DNA, RNA and proteins; gene

targeting and expression; PCR; RT PCR; southern, northern and western blotting; capillary array

sequencing; ELISA screening; use of visible and UV spectrophotometer. Radioactive handling, maintaining

lab safety.

STANDARD MICROBIOLOGY TECHNIQUES: organism isolation and purification, serotyping, culture and

media preparation. Vytek II and Gen probe diagnostic platforms.

Comfortable working on in vivo studies; basic tissue culture techniques; histological techniques including

whole mount in situ hybridization, x gal staining, immounohistochemistry, tissue embedding, and sectioning.

Excellent leadership, communication and interpersonal skills; ability to build rapport with other lab

professionals, and succeed in high pressure, challenging and deadline driven environments .

INSTRUMENTS & PLATFORMS

Applied Bio Systems: PCR Machine; 7900, 7700, 7500 Real Time PCR Machines; ABI PRISM 373 DNA Sequencer

Beckman Coulter: CEQ Sequencer 8000

Bio Rad: Bio Rad My Cycler thermal cycler CFX96

Cepheid: Smart Cycler

Eppendorf: epMotion Nucleic Acid Extraction Station; PCR Machine; Master Cycler Gradient

Qiagen: Qiagen Bio Robot MDx Workstation; Pyro Mark Q24

Tecan: Liquid Handling & Robotics

Roche: Magna Pure Extraction Workstation; Light Cycler 2.0; 480 Real Time PCR System Light Cycler

Xenogen: IVIS 200 Optical In Vivo Imaging System

PROFESSIONAL EXPERIENCE

LZ Therapeutic Group, Malvern, PA 10/2013 Present

Tissue Culture Technician (Contract Position with Aerotek)

Culture, expand and bank primary cells as well as cell lines. Prepare reagents, media and stock solutions .

Ensure the laboratory is well stocked and resourced. Maintain and operate standard laboratory equipment; such

as centrifuges, microscopes, pipetters, and pH meters. Trouble shoot equipment problems and conduct basic

repairs. Maintain refrigerators and freezers; defrosting as needed, keeping shelves organized, tracking

inventory. Carry out routine tasks accurately and following strict methodologies to carry out analyses. On

occasion perform laboratory tests to support scientific investigations. Summarize results into tables and graphs.

Conduct searches on identified topics relevant to the research.

Merck & Co. Inc., West Point, PA 6/2012 – 02/2013

IRIS FONTAINE GLOVER

230 Buffington Road Upper Chichester, PA 19014 Phone: 484-***-**** acb1y0@r.postjobfree.com

Biologist II (Contract Position with Eurofins Lancaster Laboratories)

Providing routine analytical support for in line product improvement studies using q PCR and liquid handling

automation; performing mammalian tissue culture; maintaining records and test results using Electronic

Laboratory Notebook (ELN); following good manufacturing practices.

IRIS FONTAINE GLOVER

230 Buffington Road Upper Chichester, PA 19014 Phone: 484-***-**** acb1y0@r.postjobfree.com

DELAWARE PUBLIC HEALTH LABORATORY, Smyrna, DE 5/2010 5/2011

Microbiologist 2 (Contract Position with Lab Support)

Certified Multiple Loci VNTR Analysis (MLVA) sequencing protocols for two serotypes of Salmonella, E.coli and

Listeria bacteria. Certified to run Pulse Field Gel Electrophoresis (PFGE) for Salmonella, Shigella, E.coli and

Listeria bacteria. Operated, maintained, calibrated and assisted with troubleshooting computerized and non

computerized laboratory equipment such as automated extraction systems, amplification platforms, (2) types

Real Time PCR platforms, centrifuges, incubators, etc. Work in Bio safety Level 2 and Bio safety Level 3.

PENNSYLVANIA DEPARTMENT OF HEALTH, Lionville, PA 10/2009 – 7/2010

Microbiologist 2 (Contract Position with PMHC)

Performed Real Time PCR for Influenza Virus, Norovirus, Mumps, Adenovirus and West Nile Virus. Performed

microscopic examinations of laboratory specimens to determine the presence, identity and quantity of bacteria,

fungi, protozoa or other microorganisms in any phase of their development. Work in Bio safety Level 2 and Bio

safety Level 3. Sole responsibility for conducting research to develop and modify standard operating

procedures, and by performing advanced microbiological analysis through the use of advanced testing

procedures.

DIAGNOCURE ONCOLOGY LABORATORIES, West Chester, PA 2/2008 –

8/2008

Sr. Research Technologist

Used state of the art technologies to analyze patient samples and provided test results that helped physicians

and patients better understand the risk of recurrence of the patient’s colorectal cancer. Set up the molecular

biology laboratory, modified existing Standard Operating Procedures to make them more efficient. Managed

and trained six new and existing laboratory staff.

XENOGEN BIOSCIENCES, Cranbury, NJ 9/2006 –

2/2008

Research Technician III, Metabolic Group Team Leader Phenotype Department, Metabolism Group

Evaluated metabolism in mice pre and post the introduction of a high fat diet. Data from several diagnostic

levels, that affect metabolism, are gathered to compare knockout versus wild type groups of mice.

Responsibilities included: SSOP data analysis, loading data in the client database and making the schedule for

testing of the mouse knockout strains.

XENOGEN BIOSCIENCES, Cranbury, NJ 10/2003 –

8/2006

Research Technician II Molecular Biology Group

Screened genomic DNA of using Polymerase Chain Reaction (PCR), Southern Blot and/or Slot Blot, Taqman

real time PCR, and entered results in the database. Extracted genomic DNA from mouse tail for various

projects. Purified DNA from cell cultures samples. Prepared vectors and DNA to be cloned by digestion with

restriction enzymes to generate complementary ends. Maintained ES Cell lines and prepared cells for injection

into mouse blastocysts.

IRIS FONTAINE GLOVER

230 Buffington Road Upper Chichester, PA 19014 Phone: 484-***-**** acb1y0@r.postjobfree.com

AMERICAN RED CROSS BLOOD SERVICES, Philadelphia, PA 4/2001 –

3/2002

Senior Technologist l Human Leukocyte Antigen/Molecular Genetics Laboratory

Performed PCR testing, Southern slot blot SOP, DNA sequencing and antibody screening to cross match HLA

alleles of donors and patients. Implemented new procedures and became familiar with the various computer

software related to data analysis. Assisted in the training of new lab personnel. Participated in providing HLA

services for various transplant programs.

UNIVERSITY OF PENNSYLVANIA SCHOOL OF MEDICINE, Philadelphia, PA 3/1998 – 4/2001

Research Technician Department of Cell and Developmental Biology

Conducted research to study the role a family of winged helix genes have on development of nervous tissue in

mice. Input and analyzed data, organized and maintained general lab DNA and glycerol stocks; maintained ES

Cell lines and prepared cells for injection into mouse blastocysts; maintained and ordered supplies;

communicated with researchers and other staff regarding ordering and shipment of mice; responsible for

radioactive handling and safety, maintained lab safety; and trained seven new hires and student volunteers.

EDUCATION

B.S. BIOLOGY, University of Pittsburgh, Pittsburgh, PA, 1994

Minor: Chemistry

PUBLICATIONS

Kloetzli, J.M., Fontaine, I.A., Brown, E.R., Kuo, M. and P.A. Labosky. (2000). The winged helix gene, Foxb1, controls

development of both mammary glands and regions of the CNS that regulate the milk ejection reflex.

http://www3.interscience.wiley.com/cgi bin/abstract/76510947/START Genesis 29:60 71.

Hanna, L.A., Foreman, R.K., Fontaine, I.A., Tarasenko, I.A., Kessler, D.S. and P.A. Labosky. (2002). Requirement for Foxd3 in

establishing pluripotent stem cells of the early mouse

embryo. http://www.genesdev.org/cgi/content/full/16/20/2650 Genes and Dev. 16:2650 2661.

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