Resume: Jocelyn Gile

Job Objective: Lab Technician

Education: Bachelor of Science, Idaho State University.

I am an experienced, resourceful, highly adaptive, collaborative scientist with five years of experience in

an environmental lab.

StrengthsFinders Strengths: Positivity, Empathy, Maximizer, Strategic, and Ideation

Current employment:

Since February 2009, I have been employed at the University of Victoria in the Department of Biology in

the Water and Aquatic Sciences Research Program, which is a world-class, internationally collaborative

laboratory. I hold a position as both a research and administrative assistant. As a research assistant, I

receive and prepare environmental samples for analysis, some of which I prepare for others in our lab to

analyze and some that I analyze myself (ammonium, chlorophyll, silica, turbidity, conductivity and pH). I

prepare a wide range of samples for stable isotope analysis or for detection of chemicals (petroleum

hydrocarbons, HAAs, fatty acids, lipids, pigments, caffeine, pharmaceuticals) or bacteria (coliforms). My

achievements in this area include improvements in the preparation of samples for caffeine and ammonium

analysis. As the lab’s administrative assistant, I am responsible for a great deal of the communications

with our clients, as well as the lab’s paperwork, invoicing, accounting and ordering, and resource

management. In addition I ensure that employee contracts and training are documented. My achievements

in this area include improving the traceability of purchases and cheque deposits for audits.

Previous Employment:

From 2003 through 2008, I used my skills as a well-versed native English-speaking scientist and applied

them as a freelance consultant to scientists overseas. Primarily, I edited manuscripts for publication in

journals. As an editor and English teacher, I acted confidentially to advance the careers of my clients in

the general fields of medicine, genetics, biochemistry, biophysics, microbiology, nursing and dentistry to

name a few. As a consultant, I arranged all of the international communications for the highly successful

International Congress on Childhood Cancer in 2006.

I worked at Abbott Diagnostic Division of Abbott Laboratories (Abbott Park, Illinois) as a Senior Product

Specialist from 2000 to 2002 supporting the Hepatitis A diagnostic kit, used in clinical laboratories

worldwide. In the performance of my many duties, I was the GMP (good manufacturing practices)

coordinator for the Hepatitis A Business Team. The improvement in my team members’ morale and

collaboration during FDA audits was not only a great indicator of my success, but also resulted in passing

our audits. In addition to many other duties, I took part in high level investigations of the Hepatitis A

diagnostic kit and its component products for the AxSym platform. I created and amended SOPs and

corrective actions, circulating them for approval and providing explanations as needed.

More Experience

At the Rush Medical Center (Chicago, Illinois; 1997-2000) Dept of Microbiology and Immunology and

the Flow Cytometry Lab, I worked as a laboratory technologist in both research and clinical settings. Our

work on the nationwide WIHS (Women Interagency HIV Study) project was instrumental in discovering

many important findings about HIV. In these settings, I used blood separation, cell culture, beta

scintillation, ELISA, RT-PCR and flow cytometry techniques to provide data to help determine each

participant’s immunological status and prepare and store samples for further research and testing.

In my work at the University of Texas (Houston, TX) Analytical Chemistry Center, a reference lab for

mass spectrometry, GCMS and HPLC, I was privileged to share in the development of a groundbreaking

analytical technique that has since been applied to cancer diagnostics, among other uses. Using my skill

in small animal brain dissection along with information gained in collaboration with others in the ACA

team, I was able to find a means to print the peptides and proteins of the pituitary of a rat onto to a C18

bead membrane and detect and map them using a MALDI mass spectrometry. I co-authored the

manuscript* that reported our work and Dr. Caprioli later patented and opened a new area of application

for mass spectrometry in the field of medicine.

During my studies in graduate school at the University of Texas Medical Branch at the Dept of Pathology,

I tested the Rocky Mountain spotted fever antibodies developed in Dr. David Walker’s lab on known

infected tissue from the John Sealy Hospital Histopathology tissue bank. I sectioned the paraffin fixed

tissue and used an ABC-DAB kit. The work was published, confirming the utility of these antibodies in

the diagnosis of patients.

Working with Dr. Golda Kevetter Leonard at the University of Texas Medical Branch at the Dept. of

Otolaryngology was a real learning opportunity. In order to determine the pathways of the ocular motor

neuron in gerbils, I performed survival stereotaxic brain surgery and injected neural tracers. After the

tracer had traveled through the nerves, I sacrificed the animal, perfused with saline and formalin and

dissected and preserved the brain. I then sectioned the brain, and visualized the tracer and tissue using

avidin/biotin complex (ABC/DAB) and histological stains.

Publication: Richard M. Caprioli, Terry Farmer and Jocelyn Gile, "Molecular Imaging of Biological

Samples: Localization of Peptides and Proteins Using MALDI-TOF MS", Anal. Chem. 69 (1997) 4751-

4760.

References:

1) Golda Leonard, PhD: Associate Dean for Academic Affairs, College of Pharmacy & Health Sciences,

Texas Southern University, Houston, TX. Phone: 713-***-****; e-mail: acb123@r.postjobfree.com

2) Richard Caprioli, PhD: Professor, Director of the Mass Spectrometry Research Center, Vanderbilt

University School of Medicine, Nashville, TN. Phone: 615-***-****; e-mail:

acb123@r.postjobfree.com.

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