Cell Biologist

CURRICULUM VITAE

Priya Choudhury, M.S., Ph.D.

**** ******** *****, #***

Houston, TX 77025

Tel: 713-***-****

E-mail: acap5x@r.postjobfree.com

SUMMARY OF QUALIFICATIONS:

Area of expertise is Cell and Molecular Biology

Good working knowledge of cell and tissue culture

Excellent verbal and written communication skills

Exposure to legal aspects of Intellectual Property

Certified Electronic Health Record Specialist

EDUCATION:

University of South Carolina School of Medicine 1995 PhD in Biomedical Sciences

Dept of Micro & Immunology, Columbia, SC

S.N.D.T University, Bombay, India 1985 MS in Analytical and

Medicinal Chemistry

Bombay University, Bombay, India 1983 BS in Microbiology

TECHNICAL SKILLS:

Molecular Biology Techniques: cDNA and PCR cloning, plasmid isolation, RNA isolation,

Insitu hybridization, in vitro translation, epitope tagging and transfections, sequence analysis

using nucleotide and protein sequences.

Protein & Biochemical Techniques: Gel electrophoresis, western blot analysis, sucrose density

gradient, sub-cellular fractionation, permeabilization with digitonin and in vitro reconstitution,

protease protection assays, gel chromatography and HPLC, recombinant protein purification.

Immunological Techniques: Immunofluorescence, sequential immunoprecipitation, ELISA,

lymphocyte transformation test, in vivo assay of delayed type hypersensitivity reaction by study

of mouse footpad inoculation, Fc receptor assay, in vitro collagen gel contraction, cell adhesion

and neutrophil cytotoxicity assays.

Cell and Tissue Culture: Primary culture of retinal pigment epithelial cells from human eyes,

culture of murine macrophages, perfusion of liver and generation of primary hepatocytes, breast

cancer cell lines

AWARDS AND HONORS:

July 2000 Young Investigator Fellowship Award from the Alpha One

Foundation

July 1998- Postdoctoral Training Fellowship from the American Lung

June 2000 Association

Feb 1995 Retina Research Foundation Travel Fellowship Grant from the

Association for Research In Vision and Ophthalmology

Dec 1993 Predoctoral Student Travel Award from the American Society

Of Cell Biology Education Committee

July 1992 Grant-in-Aid of Research from the National Academy of

Sciences through Sigma Xi, The Scientific Research Society

PROFESSIONAL EXPERIENCE:

2012- present: Research Assistant, Baylor College of Medicine, Houston, TX

PI: Dr Ergun Sahin, Assistant Professor, Huffington Center on Aging

Project: Role of telomeres in aging

2008-2011: Research Associate, Institute of Biosciences and Technology, Texas A & M

Health Science Center, Houston, TX

PI: Dr M. Gabriela Bowden, Assistant Professor, Center for Infectious

and Inflammatory Diseases

Project: Study of the role of the toxin Panton Valentine Leukocidin in the pathogenesis of

Staphylococcus aureus

2007-2008: Adjunct Instructor, Houston Community College, Houston TX

Project: Taught General Biology and Cell Biology

2005-2007: Research Associate, Baylor College of Medicine, Houston, TX

PI: Dr Dean E. Edwards, Professor, Department of Molecular and Cellular Biology

Project: Study of Progesterone receptor and estrogen receptor in breast cancer

2002-2005: Post-Doctoral Fellow (NIH Training Grant) Baylor College of Medicine

Houston, TX

PI: Dr C. Kent Osborne, Director, Breast Cancer Center

Project: Role of AIB1 (Amplified in breast cancer 1), the estrogen receptor co-activator

in tamoxifen resistance and breast cancer, its down regulation and impact on tumor growth

2001-2002: Research Associate, Baylor College of Medicine, Houston, TX

PI: Dr David D. Moore, Professor, Department of Molecular and Cellular Biology

Project: Cellular and molecular mechanism involved in the nuclear translocation of the

Constitutive Androstane Receptor (CAR), a transcription factor and xenobiotic receptor that

contributes to the metabolic response by activation of the cytochrome P450 (CYP) genes.

1995-2000: Post-Doctoral Associate, Baylor College of Medicine, Houston, TX

PI: Dr Richard N. Sifers, Associate Professor, Department of Pathology

Project: Secretion rescue of alpha 1-antitrypsin, Role of endoplasmic reticulum mannosidases in

directing the misfolded glycoproteins to distinct proteolytic pathways.

PUBLICATIONS:

1.Dumitrescu, O., Choudhury, P., Boisset, S., Badiou, C., Bes, M., Benito, C., Wolz, C.,

Vandenesch, F., Etienne, J., Cheung, A.L., Bowden, M.G., and Lina, G., (2011) Beta lactams

interfering with PBP1 induce Panton Valentine Leukocidin expression by triggering SarA and rot

global regulators of Staphylococcus aureus. Antimicrob .Agents. Chemotherap 1128: 01401-10.

2. Brown, E.L., Dumitrescu, O., Thomas, D., Badiou, C., Koers, E.M., Choudhury, P., Vazquez,

V., Etienne, J., Lina, G., Vandenesch, F., and Bowden, M.G. (2009) The Panton-Valentine

leukocidin vaccine protects mice against lung and skin infections caused by Staphylococcus

aureus USA300. Clin Microbiol Infect. 15(2): 156-64. Epub 2008, Dec 22.

3. Cabral, C.M., Choudhury, P., Liu, Y and Sifers, R.N. (2000) Endoplasmic reticulum

mannosidases regulate the partitioning of a secretion-impaired glycoprotein into distinct

proteolytic systems. J.Biol. Chem. 275(32): 250**-*****

4. Liu, Y, Choudhury, P., Cabral, C.M. and Sifers, R.N. (1999) Oligosaccharide modification in

the early secretory pathway directs the selection of a misfolded glycoprotein for degradation by

the proteasome. J. Biol. Chem. 274 (9): 5861-5867

5. Choudhury, P., Liu, Y., and Sifers, R.N. (1997) Quality control of protein folding

participation in human diseases. News Physiol. Sci. 12, 162-166

6. Choudhury, P., Liu, Y., Bick, R.J. and Sifers, R.N. (1997) Intracellular association between

UDP glucose: glycoprotein glucosyltransferase and an incompletely folded variant of alpha 1-

antitrypsin. J. Biol. Chem. 272 (20): 134**-*****.

7. Liu, Y., Choudhury, P., Cabral, C.M. and Sifers, R.N. (1997) Intracellular disposal of

incompletely folded human alpha 1-antitrypsin involves release from calnexin and post-

translational trimming of asparagine-linked oligosaccharides. J. Biol. Chem. 272 (12): 7946-

7951

8. Choudhury, P., Chen, W.H., Hunt, R.C. (1997) Production of platelet derived growth factor

by interleukin-1 beta and transforming growth factor-beta-stimulated retinal pigment epithelial

cells lead to contraction of collagen gels. Invest. Opthalmol. Vis. Sci. 38 (5): 824-833.

9. Hunt R.C., Pakalnis, V.A., Choudhury, P., Black, E.P. (1994) Cytokines and serum cause

α2β 1 integrin mediated contraction of collagen gels by cultured retinal pigment epithelial cells.

Invest Ophthalmol. Vis. Sci. 35: 955-963.

10. Hunt R.C., Fox, A., Pakalnis, V.A., Sigel, M.M., Kosnosky, W., Choudhury, P., Black, E.P.

(1993) Cytokines cause cultured retinal pigment epithelial cells to secrete metalloproteinases and

contract collagen gels. Invest Ophthalmol. Vis. Sci. 34: 3179-3186.

INVITED LECTURE:

Presentation titled ‘Quality Control in the Secretory Pathway of Alpha 1-Antitrypsin’ at the

Texas Thoracic Society Meeting on April 14-15, 2000 in Austin, Texas

REFERENCES:

Available on request

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