Scientist, spectroscopy cytometry chemistry biology
Resume:
TATYANA CHERNENKO, PhD San Jose, CA *****
****.*********@*****.***
http://ebi.cbst.ucdavis.edu
PROFILE
Research experience encompasses fields from physical chemistry to pharmaceutical sciences. Current a t-
tention is focused on Raman Imaging as well as instrumentation development for Flow Cytometry variant
system as well as protein chemistry for synergistic reagent development. Previous work was focused on
implementation of non-invasive spectroscopic imaging modalities on various biological systems in vitro, as
well as synthesizing biocompatible nano-pharmaceuticals to follow their sub-cellular trafficking and load-
release patterns.
EDUCATION
Northeastern University, Boston, MA PhD 2010 in Physical Chemistry and concentration in Nanomedicine
Department of Chemistry and Chemical Biology
Major Research interest was in imaging modalities and micro -spectroscopic methods of analysis
PhD Dissertation Title: ”Non-Invasive Label-Free Imaging of Sub-Cellular Architecture and Intracel-
lular Behavior of Nano-Drug-Delivery Carriers Using Raman Micro-Spectroscopy” in the laboratory
of Prof Max Diem.
Hunter College of CUNY, New York, NY BA 2006 in Biochemistry with minor in Biology
Activities April 2004-May 2006: Main focus of research was on the proliferation cycle of individual
cervical cancer cells.
SKILLS
Worked with the following techniques and laboratory equipment: Cell culture, blood work, tissue handling,
conjugation chemistry, antibody-conjugation chemistry, SDS-PAGE, Western Blot analysis, immunestain-
ing, and performed dose-dependent studies; Flow Cytometry, Sorting, HPLC, Raman and FTIR spectrosco-
py, Fluorescence Microscopy, UV/Vis absorption. Utilized the following programs in r esearch: MATLAB,
Cache.
EXPERIENCE
POSTDOCTORAL RESEARCH SCHOLAR AND ENTREPRENEURIAL FELLOW, UC DAVIS AND BD BIO-
SCIENCES, SACRAMENTO & SAN JOSE, CA, 2012-PRESENT
Worked in collaboration with BD Bioscience chemists and engineers to develop a new Flow C y-
tometry system. In parallel, worked with conventional Flow Cytometry systems to verify and perform
quality control on metal nanoparticle conjugates for future implementation as a method of detect ion.
Functionalized and analyzed metal nanoparticle conjugates with various antibodies as modific a-
tions, for subsequent multiplexing capabilities. Implementing protein conjugation chemistry for n a-
noparticle modification and validating with conventional analytical techniques.
POSTDOCTORAL RESEARCH ASSOCIATE, NORTHEASTERN UNIVERSITY, BOSTON, MA 2010-
2012
Department of Pharmaceutical Sciences and Department of Chemistry and Chemical Biology
Optimized preparation and administration of biodegradable nanopart iculates. Implemented sub-
micron non-invasive spectroscopic methods of analysis to conduct in vitro cellular trafficking stud-
ies, monitor cellular responses to nanoparticle administration, as well as establish intracellular n a-
noparticle penetration and degradation patterns with use of the label -free imaging methods. Per-
formed subsequent data analyses using Matlab.
Synthesized and characterized polymeric and biocompatible nanoparticles for analysis of their i n-
tracellular trafficking and dynamics. Synthesized and functionalized polymer-blend nanoparticles
with hetero-bi-functional poly(ethyleneglycol) spacer and utilized peptide conjugation chemistry to
create targeted polymer blend nanoparticles.
Implemented Raman micro-spectral modality as well as Fluorescence imaging in order to validate
and characterize the tumor micro-environments.
Techniques utilized for nanoparticle formulation: particle sizing, zeta potential measurement, HPLC,
Raman and IR spectroscopic analyses as well as optical imaging.
RESEARCH ASSISTANT, NORTHEASTERN UNIVERSITY, BOSTON, MA 2007-2010
IGERT Nanomedicine Science and Technology
Led projects focusing on pharmaceutical nano -carriers, including liposomes, micelles, and various
polymer-based nanoparticles. Established their drug rele ase kinetics as well as intracellular dynam-
ics and patterns of degradation. Monitored dissociation patterns of the drug -payload using Raman
micro-spectroscopy as a non-invasive and label-free method of detection.
Characterized nanoparticles using SEM, AFM microscopy, fluorescence spectroscopy, particle
size, and zeta-potential measurements.
Familiar with different nanotechnology platforms and applications for targeted detection, drug deli v-
ery, and molecular imaging.
Department of Chemistry and Chemical Biology
Led projects involving establishment of biochemical patterns of the aging mouse oocyte based on
monitoring the mitochondrial intra-cellular distribution patterns.
Performed key analyses for a Mass Eye and Ear Infirmary Angiogenesis Laboratory -based project
involving label-free imaging of mouse corneas during growth -factor-induced angiogenesis. Tech-
niques heavily relied on were Raman Microspectroscopy and fluorescence spectroscopy.
Conducted other projects: analysis of large tissue sections using IR s pectroscopy, Raman micro-
spectral imaging, Fluorescence (2 Photon, Confocal imaging, SHG), also worked with artificial skin
samples and their subsequent imaging with OCT.
RESEARCH ASSISTANT, HUNTER COLLEGE OF CUNY, NEW YORK, NY 2004-2006
Worked with FTIR spectroscopic method of analysis in order to verify and establish biochemical signatures
of the cell cycle as well as characterize cancerous regions from tissue biopsies. Performed immunohist o-
chemical stains and other commonly used fluorescence staining procedures for cross-validation.
NURSE’S ASSISTANT, CONEY ISLAND HOSPITAL, BROOKLYN, NY 2001-2003
Worked alongside nurses and doctors, assisting in words such as the recovery unit, rehabilitation, neonatal
care, OB/GYN, and pediatrics. Position required paper-work filing on patient history and assisting nurses in
patient care.
TEACHING EXPERIENCE/OUTREACH
Volunteer Science Teacher Boston, MA Summer
2007
Participated in outreach platform “Introduction to the NanoWorld” for local K -12 students
Graduate Teaching Assistant, Northeastern University Boston, MA 2006 -
2007
Position required teaching physical chemistry laboratory and checking laboratory reports, working
problems and evaluating students’ performances.
Staff Tutor, Math & Science Partnership New York, N Y 2006-
2006
Tutored high school students individually or in a classroom setting in order to prepare them to su c-
cessfully pass the Regents Examinations. Responsibilities included answering questions, problem
solving, and reviewing study topics. Subjects of focus: physics, chemistry, b iology and environ-
mental science.
Academic Assistant, Hunter College, Office of AccessABILITY New York, NY 2004 -
2005
Page 2
Took notes on behalf of disabled students for a variety of classes such as Mathematics of Matrices,
Economics, and Foundation of US Constitution; also responsible for proctoring exams.
AWARDS AND RECOGNITION
07/2012 Certificate of completion of Biomedical Engineering and Entrepreneurship Academy
at University of California, Davis
07/2012 Certificate of Recognition awarded for Contributions to Center for Biophotonics Sc i-
ence and Technology (CBST)
11/2011 Invited speaker at Martinos center for Biomedical Imaging at Massachusetts General
Hospital, Harvard Medical School, Boston MA
08/2011 Speaker at annual Controlled Release Society (CRS) Conference 2011 in Washin g-
ton DC
06/2011 Speaker at annual NanoTech Conference & Expo in Boston, MA 2011
02/2011 Invited lecturer to University of Texas, Department of Pharmaceutical Sciences.
08/2010 Member of the Editorial Board of Journal of Molecular Imaging & Dynamics.
04/2010 Biography to be published in the 2011 edition of Marquis Who's Who in America.
03/2009 Invited lecturer for Massachusetts Bay Community College.
02/2008 Invited speaker at 3rd Annual GML Intercollegiate Symposium on Interdisciplinary
Graduate Research.
05/2007-2009 NSF Integrative Graduate Education and Research Traineeship (IGERT) fellowship
in Nanomedicine Science and Technology for work in Non -Invasive Imaging of
Nano-Drug Delivery carriers.
10/2004 Received Fannie Margolies Roberts scholar award in Chemistry at Hunter College of
CUNY.
PUBLICATIONS
Articles:
Chernenko, T., Miljkovic, M., Diem, M., Amiji, M. “Non -targeted and EGFR-targeted nanoparticles monitored
by label-free Raman micro-spectral imaging analysis” In Preparation.
Nakao S, Zandi S, Taher M, Schering A, Chernenko T, Matthäus C, Hafezi -Moghadam. “Diet dependent
Mechanism of Selective Lymph angiogenesis” Article in preparation.
Chernenko, T., Sawant, R., Miljkovic, M., Quintero, L., Diem, M., Torchilin, V. “Raman Microscopy for Non -
Invasive Imaging of Pharmaceutical Nanocarriers: Intracellular Distribution of Liposomes of Different
Composition” Molecular Pharmaceutics 9: 930-***-****
Majed, N., Chernenko, T., Diem, M., Gu, A. "Identification of Functionally Relevant Populations in Enhanced
Biological Phosphorus Removal Processes Based On Intracellular Polymers Profiles and Insights into
the Metabolic Diversity and Heterogeneity" Environmental Science & Technology 46: 501*-****-****
Ismail, M., Ibe, U., Chernenko, T., Diem, M., W arzywoda, J., Sacco, A.”Synthesis and characterization of
vanadosilicate AM-6 with transition metal ions isomorphously substituted in the framework” Microporous
and Mesoporous Materials, 135: 118-***-****
Page 3
Cherchi, C., Chernenko, T., Diem, M., Gu, A. “Impact of nTiO2 Exposure on Cellular Structure of Anabaena
variabilis and Evidence of Internalization” Environmental Toxicology and Chemistry, 30: 861-***-****
Miljkovic, M., Chernenko, T., Romeo, M., Bird, B., Matthäus, C., Diem., M. “Label -free imaging of human
cells: algorithms for image reconstruction of Raman hyperspectral datasets” The Analyst, 135: 2002-
2013 2010
Zuser, E., Chernenko, T., Newmark, J., Miljkovic, M., Diem, M. “Confocal Raman microspectral imaging
(CRMI) of murine stem cell colonies” The Analyst, 135: 303*-****-****
Chernenko, T., Sawant, R., Matthäus, C., Quintero, L., Torchilin, V., Diem, M. “A Novel Approach to Follow
the Intracellular Fate of Pharmaceutical Nanocarriers” Controlled Release Society Newsletter, 27: 18-19
2010
Chernenko, T., Matthäus, C., Milane, L., Quintero, L., Amiji, M., Diem, M. “Label-Free Raman Spectral Imag-
ing of Intracellular Delivery and Degradation of Polymeric Nanoparticle Systems ” ACS Nano, 3: 3552-
3559 2009
Matthäus, C., Chernenko, T., Milane, L., Ka le, A., Quintero, L., Amiji, M., Torchilin, V., Diem, M. “Raman
Microscopy Imaging of Nanocarrier Systems for Cellular Uptake and Trafficking” Controlled Release
Society Newsletter, 26: 13-14 2009
Wood B. R., Chernenko T., Matthäus C., Diem M., Chong C., Bernhard U., Jene C., Brandli A. A., McNaugh-
ton D., Tobin M. J., Trounson A., Lacham -Kaplan O. “Shedding New Light on the Molecular Architecture
of Oocytes Using a Combination of Fourier-Transform Infrared and Raman Spectroscopic Mapping”
Analytical Chemistry, 80: 906*-****-****
Matthäus, C., Chernenko, T., Quintero, L., Milane, L., Kale, A., Amiji, M., Torchilin, V., Diem, M. “Raman
Microscopic Imaging of Cells and Applications Monitoring the Uptake of Drug Delivery Systems” Proc.
SPIE, 6991: 699***-****
Matthäus C, Kale A, Chernenko T, Torchilin V, Diem M. “New Ways of Imaging Uptake and Intracellular Fate
of Liposomal Drug Carrier Systems inside Individual Cells, Based on Raman Microscopy” Molecular
Pharmaceutics, 5: 287 2008
Matthäus, C., Chernenko, T., Newmark, J., Warner, C., Diem, M. “Label-free detection of mitochondrial dis-
tribution in cells by nonresonant Raman Micro-Spectroscopy” Biophysical Journal, 93: 668 2007
Boydston-White, S., Romeo, M., Chernenko, T., Regina, A., Miljkovic, M., Diem, M. "C ell-cycle-dependent
variations in FTIR micro-spectra of single proliferating HeLa cells: Principal component and artificial
neural network analysis." Biochimica et Biophysica Acta (BBA) - Biomembranes, 1758: 908-***-****
Boydston-White, S., Chernenko, T., Regina, A., Miljkovic, M., Matthaus, C., Diem, M. “Misrospectroscopy of
Single Proliferating HeLa Cells.” Vibrational Spectroscopy, 38: 169-***-****
Books and Book Chapters:
Chernenko, T., Diem, M., Torchilin, V. “Raman micro-spectroscopy as a non-invasive tool to follow the intra-
cellular fate of nanoparticles”, in “Frontiers of Nanobiomedial Research”, World Scientific Publishing. In
Press.
Chernenko, T., Milane, L., Matthäus, C., Diem, M., Amiji, M. “Raman Microspectral Imaging for Label-Free
Detection of Nanoparticle-Mediated Cellular and Sub-Cellular Drug Delivery”, in “Drug Delivery Applica-
tions of Noninvasive Imaging: Validation from Biodistribution to Sites of Action”, E ditors Edward F.
Jackson, Mei Tian, Chun Li. In press.
Matthäus C., Chernenko T., Quintero L., Miljković M., Milane L., Kale A., Amiji M., Torchilin V., Diem M.:
“Raman Microscopic Imaging of Cells and Applications Monitoring the Uptake of Drug Del ivery Sys-
tems”, in “Confocal Raman Microscopy”, Editors: Dieing T., Toporski J., Hollricher O., Springer Verlag,
Heidelberg, Germany, 2011, pp.137-164.
Chernenko, T. “Biochemical Imaging of Drug-Delivery Systems Using Raman Micro-Spectroscopy to Probe
Cell Architecture and Behaviors of Nanoparticles” LAP LAMBERT Academic Publishing AG & Co.KG,
Saarbrucken. 2010
Diem M., Matthäus C., Chernenko T., Romeo M. J., Miljković M., Bird B., Schubert J., Papamarkakis K.,
Bedrossian K., Laver N.: “Infrared and Raman Spectroscopy and Spectral Imaging of Individual Cells”,
in “Infrared and Raman Spectroscopic Imaging”, Editors: Salzer R., Siesler H. W., Wiley - VCH Publish-
ing, 2009
Diem M., Matthäus C., Miljković M.,, Chernenko T., Romeo M., Bird B.: “Infrared and Raman Microscopy in
Cell Biology”, in “Methods in Cell Biology, Volume 2: Biophysical Techniques”, Editors: Correia J. J.,
Detrich H. W., Elsevier, 2008, pp. 276-308.
Page 4
Romeo M. J., Bird B., Boydston-White S., Matthäus C., Miljković M., Chernenko T., Diem M.: “Infrared and
Raman Micro-spectroscopic Studies of Individual Human Cells”, in “Vibrational Spectroscopy for Med i-
cal Diagnosis”, Editors: Diem M., Griffiths P., Chalmers J., J.Wiley & Sons Ltd., West Sussex, UK, 2008,
pp. 27-70.
Romeo M. J., Boydston-White S., Matthäus C., Miljković M., Bird B., Chernenko T., Diem M.: “Vibrational
Microspectroscopy of Cells and Tissues”, in “Biomedical Vibrational Spectroscopy”, Edit ors: Lasch P.,
Kneipp J., J.Wiley & Sons Inc., Hoboken, NJ, USA, 2008, pp.121-152.
REFERENCES
Available upon request.
Page 5
Contact this candidate