Design Development

Laurent-Michel VUILLARD

French National, DOB : ** 11 1960, Married, 2 children (age 23+25).

Bilingual English + French; basic German.

Contact address: *** *** *****, ***** *******, France

Tel: home +**-**-**-**-**-**. Work + mobile: +**-**-**-**-**-**

***************@*******.***

Management experience:

- Leading a team of 5 to 10 scientists (from technical staff to

experienced PhDs) for delivery of key subcontracting results to

larger pharmas.

- Dealing with customers for project definition and negotiation of

deliverables, timelines and prices.

- Managing reporting and communication to customers and management.

- Safety issues (incl. radiotracers).

- Lab budget supervision and cost cutting.

- EU funding applications and French tax credit.

- Setting up and running collaborations with academic teams.

Field of Expertise: Design, production and assay of proteins in the drug

development process.

. Design of protein constructs for structure determination or HTP assays

:

Transforming the complex drug targets identified by cellular biology

to into constructs suitable for production, drug development and

screening:

. Development of banks of antibody like binder-proteins (Scfv and

original scaffolds).

- Validation of the scaffolds.

- Display and screening methods.

- Binder validation.

. Production :

Design and implementation of dependable protein production processes:

- Expression (E.Coli to mammalian cells).

- Purification : analytical scale to upstream production process

from recombinant or natural proteins.

- Protein refolding.

. Structure determination :

Whole process from construct to 3d structure (x-ray or NMR) applied to

drug discovery with a focus on the identification of the drug binding

sites.

. Protein QC:

Ensuring the suitability of the constructs for DD or structural use:

- Biochemical assays and validation.

- Biophysical methods : Mass spectrometry, Electrophoresis (incl 2d),

Thermofluorimetry, Light scattering DLS MALS, angle x-ray (or

neutron) scattering, NMR.

. Design and validation of activity assays in-vitro :

- Setup of assays using radiotracers or absorbance or fluorescence;

(ex Gef proteins, Kinases). Ubiquitination assays. Fluorescence

polarization.

- Setup and transfer to customer of a luminescence assay to replace

radioactive assays; (e.g. t-RNA synthetase assay for the screening

of natural antibiotic compounds).

Education:

Ma trise de Biochimie-Physiologie. University of Lyons, 1985.

PhD (Biochemistry), University of Edinburgh, 1989.

Summary of work experience:

Since 2006 CSO Bioxtal SA (Research and service biotech, 10 employee

scale),

Design and Supervision of service research programmes in

structure based drug design, Contact with customers for

scientific and commercial issues. Business development.

2004 2006 Self-employed protein technology consultant. Assay

development. Setup of an automated protein refolding

process.

2002 2004 Avidis (UK+ France): Head of protein technology lab

Protein expression production of scaffold proteins in view

of therapeutic development.

2000 2002 Astex therapeutics (Cambridge, UK): Responsible for

implementing the QC for protein production (light

scattering mass spectrometry) ; Setup of medium scale

protein production and refolding for fragment based drug

discovery.

1999 2000 Postdoctoral fellow university of Cambridge.

1997 1999 Postdoctoral fellow: University of St Andrews.

1997 Short term fellow Insitut Pasteur, Paris : Validation of own

designed protein refolding agents.

1991 1996 Scientist, ILL Grenoble, biophysics of proteins; design of

new additives for protein refolding

purification and crystallisation (now commercialised by Merck

and Hampton research).

1989-1991 Postdoctoral fellow, University of Edinburgh.

Major achievements

. Turnaround of Bioxtal operations: Establishing full process from

protein production to QC and structure pipeline.

. Development of an HTP refold process to increase throughput of HTP

structural genomics.

. Setup of the QC process at Astex Therapaeutics at the beginning of the

company.

. Design of additives for protein refolding an crystallisation (now

commercial products sold by several manufacturers).

. 24 publications, Five patents applications.

References:

Upon request .

Personal interests:

Piano 19th century music, mountain hiking, history.

Contact this candidate