Assistant Management

Xin Shi

**** ************* **. *****: 832-***-**** (c)

Houston TX

***** *-****: *******@*****.***

Objectives

Seeking a full-time position as a research assistant.

Laboratory Skills

Mice handling, genotyping, gel Electrophoresis and dissection.

DNA, RNA and protein manipulation.

Light microscope.

Cell and tissue culture.

Lab management.

Work Experience

May 2010 – July 2013. Dept. of Molecular and Cellular Biology, Baylor College of Medicine

Neurosphere cell culture.

Genomic DNA, RNA and Protien isolation.

Western blot.

siRNA transfection.

Lab supply order and maintaining.

Nov. 2007- May. 2010. Dept. of Molecular and Cellular Biology, Baylor College of Medicine

Experience with mice handling.

Southern blot with both radioactive and non-radioactive method.

DNA cloning and purification.

Tissue sectioning and staining.

Lab supply order and maintaining.

Feb. 2005-Nov.2007. Dept. of Pathology, Baylor College of Medicine

Performed Mice handling including colony management and characterization.

Tissue collection, paraffin and frozen.

Dendritic cell isolation from small intestine.

Performed ELISA and data analysis.

Whole-mount preparation of small intestine lectin staining and light microscope examination.

Clonic fragment culture.

Management of the lab database.

Aug. 1984 - Dec. 1996 Dept. of Biology, Binzhou Educational College, Binzhou Shandong, China.

Assistant professor and Research associate

Teaching on general and organic chemistry classroom and laboratory.

Volunteer experience:

June 2004-Nov. 2004: Dept. of Pediatrics Nutrition CNRC, Baylor College of Medicine

Performed Mice handling, genotyping PCR, DNA extraction, tissue collection and frozen.

Preparation of solutions and gel.

Purification of DNA from agarose gel.

Bisulfite treated genomic DNA.

Manual bisulfite DNA sequencing.

Education

Associate, Chemistry. 1982-1984 Binzhou Normal College. Binzhou, Shandong, China

B.A. Chemistry. 1986-1989 Shandong Normal University. Jinan, Shandong, China

Relevant courses:

Intensive organic Chemistry, Biology, Pathology, Histology, Immunology and Statistics

Publication:

Waterland RA, Dolinoy DC, Lin JR, Shi X and Tahiliani KG. Maternal methyl supplements increase offspring DNA methylation at Axin Fused.

Genesis. 2006 Sep; 44 (9):401-6.

References: Available upon request

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