Plant Construction

Yaodong Chen, Ph.D

Email: ******@***.***

Tel: 919-***-****

Home Address:

*** ****** ******* *****, ****, NC, 27519

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• Career work has covered multidisciplinary fields in microbiology, cell biology

and plant biology over 10+ years. Excellent experience in analytical, problem

solving, and organizational skills.

• Expertise in protein biochemistry and biophysical research. Expertise in protein

purification and protein analysis.

• Proficient in recombinant DNA technology, extensive experience in recombinant

mutation construction.

• A productive independent researcher with excellent communication and computer

skills. Published 10+ research papers in Biophysics Journal, Journal of Biological

Chemistry, Biochemistry et al.

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5 TECHNICAL SKILLS:

• Protein Purification: Expertise in different chromatography techniques including Ion exchange

chromatography; Size exclusion chromatography; Reverse phase chromatography et al; Experience

with GE AKTA chromatography systems/Unicorn software. Experienced in protein purification from

bacteria, yeast, plant and mammalian cell systems. Experienced in tagged or non-tagged protein

purification.

• Molecular and Cellular Biological skills: Mutant protein construction; Western Blot, ELISA et al.

Cell culture and cell imaging et al.

• Biophysical techniques: Expertise in protein analysis using different spectra technique including

fluorescence, fluorescence anisotropy, CD, Light-scattering, Mass Spectra et al; Expertise in

protein-protein interaction analysis techniques such as Isothermal Titration Calorimetry (ITC); SEC-

MALS (Size-Exclusion Chromatography coupled with Multi-Angle Static Light Scattering) et al.

Expertise in protein structure analysis techniques such as protein X-ray crystallography. Also

expertise in Fluorescence images technique; Time-resolve spectra techniques and data analysis;

Electron microscopy techniques et al;

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7 RESEARCH EXPERIENCE

2011.10 present. Postdoctoral Research Associate, Department of Biology, University of North Carolina at

Chapel Hill

Studying the dynamic of microtubulin and the effects of its capping protein using single molecular

fluorescence technique. Studying proein-protein interaction between capping proteins and resolve their

structures. Through ITC and SEC-MALS, we found the new interaction sites between EB1 and Clip-170.

And we solved the high-resolution structure of EB1 binding SXIP motif using protein X-ray

crystallography.

2003.3 2011.10: Research Associate and Senior Research Associate in Department of Cell Biology, Duke

University Medical Center, Durham, NC

Create and execute research and development strategy to study bacterial cytoskeleton protein and

cell division. Successful setting up real-time and sensitive assays based on fluorescence technique to

study the dynamic properties of the FtsZ protein assembly and turn-over in vitro. These assays are very

useful not only for the FtsZ studies but also for the screening new anti-FtsZ compounds, which could be

used to develop novel anti-bacterial drug. Published 7 research articles in top-ranked journals.

2000.5 2003.3: Postdoctoral research in Boston Biomedical Research Institute, Watertown, MA

The major contributions include: synthesized a new Tb-chelated compound and used it as the donor

to develop the LRET (Luminescence Resonance Energy Transfer) technique to measure the distance

between tropomyosin across the muscle thin filament.

1998.11 2000.5: Assistant Professor in Photosynthesis Center, Institute of Botany, Chinese Academy of

Sciences, Beijing, China.

Studies on the primary reaction of reaction center and light-harvesting complexes of plants photosystem

II using ultra-fast spectra technique.

8 EDUCATION

1995 1999, Ph.D of Plant Physiology and Biochemistry, Institute of Botany, Chinese Academy of

Sciences, Beijing, China.

1992 1995, M.Sc of Optics and Photobiology, Institute of Optics and precise mechanism in Xi'an,

Chinese Academy of Sciences, Xi'an, China.

1988 1992, B.Sc of Microbiology, Northwest University, Xi'an, China.

PUBLICATIONS:

Yaodong Chen, Sara L. Milam, and Harold P. Erickson. SulA inhibits assembly of FtsZ by a simple

sequestration mechanism. (2012) Biochemistry. 51(14):3100-9.

Yaodong Chen and Harold P. Erickson. Conformational changes of FtsZ reported by tryptophan

mutants. (2011) Biochemistry. 50(21):4675-84.

Yaodong Chen and Harold P. Erickson. FtsZ filament dynamics at steady state subunit exchange with

and without nucleotide hydrolysis. (2009) Biochemistry. 48(28):6664-73.

Yaodong Chen and Harold P. Erickson. In vitro assembly studies of FtsZ/tubulin-like proteins from

Bacillus plasmids: evidence for a capping mechanism. (2008) Journal of Biological Chemistry.

283(13): 8102-9.

Yaodong Chen, David E. Anderson, Malini Rajagopalan and Harold P. Erickson. Assembly dynamics of

Mycobacterium tuberculosis FtsZ. (2007) Journal of Biological Chemistry. 282(38): 27736-43.

Yaodong Chen and Harold P. Erickson. Rapid in vitro assembly dynamics and subunits turnover of FtsZ

demonstrated by fluorescence resonance energy transfer. (2005) Journal of Biological Chemistry.

280(23): 22549-54.

Yaodong Chen, Keith Bjornson, Sambra D. Redick and Harold P. Erickson. A rapid fluorescence assay

for FtsZ assembly indicates cooperative assembly with a dimer nucleus. (2005) Biophysics Journal.

88: 505-514.

Yaodong Chen and Sherwin S. Lehrer. Distances between tropomyosin sites across the muscle thin

filament using luminescence resonance energy transfer: evidence for tropomyosin flexibility. (2004)

Biochemistry, 43, 114**-*****.

Yaodong Chen, JianXin Chen, Hou Jian-Min, Chen Hui, Zhang Xing-Kang, Zhang Qi-Yuan, Li Liang-

Bi and Kuang Ting-Yun. Dynamic studies on primary reactions of the PSII particles and core

complexes by femtosecond absorption spectroscopy. (1999), Acta Botanica Sinica, 41(7): 700~705.

Yaodong Chen, JianMin Hou, JianXin Chen, Qi-Yuan Zhang, Liang-Bi Li and Kuang Ting-Yun. Studies

on the PSII particles and core complexes using femtosecond absorption spectroscopy. (1998) in: (Ed.

G. Garab) Photosynthesis: Mechanism and Effects. Kluwer Acad Pub, Dordrecht, Vol I: 289~292.

Yaodong Chen, Yichun Guan, Hou Jianmin, Tang Chongqin, Wang Shuicai, Hou Xun and Kuang

Tingyun, Study on the energy transfer process between the photosystem II reaction center and light-

harvesting system. (1997), Acta Botanica Sinica, 39(4): 372-374. (in Chinese)

Shu-Qin Li, Yao-Dong Chen, Chong-Qin Tang, Liang-Bi Li, Ting-Yun Kuang. Spectral properties and

characterization of the modified PSII core complexes. (2000), Acta Botanica Sinica, 42(6):

643~645.

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