Research Assistant

VINUTHA REDDY KASIREDDYApt ***, *** Kennedy Dr, Malden, MA- 02148 ~ 612-***-**** ~

****.*********@*****.*************** SUMMARY

An academically competent student with strong command in advanced pharmaceutical sciences, pharmacology

and molecular biology. Exhibited dexterity in molecular biology related laboratory techniques and have

pharmaceutical industry exposure, adhered to GMP, GLP guidelines with proven professionalism.

EDUCATION

MASTER OF SCIENCE: PHARMACEUTICAL SCIENCES May 2013

Bouve College of Pharmacy and Health Sciences, Northeastern University, Boston, MA, United States

BACHELORS OF PHARMACY May 2011

RBVRR College of Pharmacy, Osmania University Andhra Pradesh, India

RELEVANT EXPERIENCE

TUFTS MEDICAL CENTER, Molecular Oncology Research Institute, Boston Jul 2013 – present

Research Intern, under the guidance of Principal Investigator Prof Dr Miaofen Hu, MD, PhD

• Genotyping of wild and knockout mice; Extraction and Purification of DNA and RNA

• Executing FACS analysis by flow cytometry on different cells for cell counting, sorting with biomarkers

• Sub culturing and maintaining mammalian cell lines 3T3-L1, P3-shrna iCDK6 for further analysis and

staining, Isolation of bone marrow stem cells and carrying out cell culture experiments

• Mice dissection and cryopreservation of vital immune organs, study protein expression by Western Blot

• Proficient in Nano Drop 2000C Spectrophotometer, PCR, BCA protein assay, Gel electrophoresis

• Adipose tissue differentiation and investigating the role of CDK6 in adipogenesis

• Strong theoretical and working knowledge in cancer biology and shRNA vector systems

• Data analysing, documenting and presenting the results in weekly lab meetings

BACTOLAC PHARMACEUTICALS INC., Hauppauge, New York Jul - Aug 2012

Product Research and Development Intern, Quality Control Department

• PROJECT: Chemical testing and Heavy metal testing of raw materials according to USP monographs

• Adept in handling Fourier Transform Infrared Spectroscopy, UV-Vis Spectrophotometer, ICP, HPLC for

identification of raw materials used in manufacturing Vitamin and hormonal supplements

• Trained in Polarimetry using Autopol V plus polarimeter to determine optical activity of amino acids

• Monitored qualitative analysis of raw materials, Routine Inprocess & finished product sample analysis

according to SOP’s, disintegration studies using different dissolution media; in vivo simulation fluids for

controlled and time release formulations

• Prepared Tooling Reports, Revision of protocols, updating analysis data, maintaining laboratory

notebooks and documenting raw material data sheets and test reports

SKILL HIGHLIGHTS

• Developing and validating ligand binding assays ELISA, Western blot and qPCR, transfection assays,

Bioassays of drugs on animal tissue by interpolation, three point, and four point techniques.

• Worked on mammalian cell lines of CHO-DP 4, PANC 1 in BL2 environment

• Performed CytoTox Fluor Cytotoxicity assay, Immunofluorescence assay, cDNA synthesis, plasmid

preparations

• Scaling up and scaling down mammalian cell lines

• Isolation and preservation of pure cultures, Carried out Affinity Chromatography, TLC, wet chemistry

techniques; calibrated burette, pipette; prepared buffers and reagents, stock solutions and dilutions

• Proficient in using MS Word, Excel, Power point, C Language, SQL language, HTML, Pymol

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