Quality Assurance Technician

REBECCA J. BOBULA

T: 614-***-**** E: *************@*****.***

EXPERIENCE RESEARCH TECHNICIAN/MOLECULAR ANALYST/BIOLOGIST II

Monsanto; Creve Coeur, MO

Dec ’10 - Jun ’12 (Direct); May ‘09 - Dec ’10 (Contract-Today’s Staffing)

Develop, validate, and transfer detection methods to enable global registration, stewardship, and freedom to operate for

Monsanto plant biotech products. Duties include sample handling, DNA extraction, DNA quantification and dilution,

qualitative and quantitative real-time TaqMan PCR and RPA (Recombinase Polymerase Amplification)/isothermal am-

plification optimization and validation, data analysis/documentation, report writing, SOP writing and QC review. Primary

responsibilities involve serving as resident expert on isothermal RPA technology, including: developing appropriate one

step extraction methods for various starting materials, optimizing various platforms to use the RPA technology, devel-

oping VBA Excel worksheets to analyze raw data values, determining assay cut-offs, acceptance criteria, and overall

normal characterization of reactions. Additionally, responsible for organizing and developing daily experiments to meet

project goals and timelines, and reviewing and delivering results to various teams weekly. Prepared and delivered

presentation at annual scientific conference for 200+ members of scientific community.

SCIENTIST

Pfizer Inc. (Aerotek Scientific); Chesterfield, MO

Mar ‘08 - Oct ‘08

Trained and proficient in binding assays (OX40 indirect binding ELISA), cell-based assays (cAMP Potency assay, Lu-

ciferase cAMP Potency assay, CCR2 cell based assay), cell culture, and some extraction/RT-PCR work. Worked

closely with team in R&D environment to develop robustness of assays for validation. Prepared and gave professional

presentations to colleagues and superiors in order to foster discussion of on-going work and to collaborate relevant

ideas from other scientists. Maintained accurate and traceable records of all work performed under strict GMP guide-

lines, including addressing any aberrant or unexpected results/findings by conducting thorough, regulated investiga-

tions.

SENIOR MICROBIOLOGY TECHNICIAN

Battelle Biomedical Research Center; West Jefferson, OH

Nov ‘04 - Jul ‘07 (FT); Aug ‘07 - Jun ‘08 (PT)

Specialized areas include DNA isolation and Real-Time PCR and immunological assays (ELISA and TNA) to support in

vivo pathogenicity/toxicity studies. Extensive work with highly hazardous biological materials and select agents in BSL-

3 and BSL-2 laboratories. Thorough knowledge and experience carrying out validation and qualification of biological

assays and analytical equipment. Experience with several microbiological activities including viral plaque and plaque

reduction neutralization assays, cell culture, dilutional theory, aseptic technique, and reagent preparation. Worked as

study lead on several projects which require coordination of study-related activities and serving as liaison between

technicians and study directors. Experience running and coordinating meetings with other technicians to divvy work re-

sponsibilities and delegate study activities. Independently carried out role as administrator and trainer of FDA-regulated

and GLP compliant equipment and software system. Maintained accurate and traceable records of all work performed

under strict GLP and ISO 9001 guidelines.

MICROBIOLOGIST

Nestle Quality Assurance Center (Kforce Scientific); Dublin, OH

Aug ‘03 - Oct ‘04 (FT); May ‘05 - Mar ‘07 (PT)

Daily responsibilities: ELISA, Vitek, VIDAS, reading and interpreting plate and tube results, serology of Salmonella spe-

cies, transfer of samples to appropriate media based on organism of interest and food type, strict attention to incubation

times, determining dilutions and weighing samples into media. Weekly: responsible for stocking and ordering all sup-

plies for pathogen lab. Follow SOPs according to all microbiology-related activities, water quality, in

house/environmental swabs, waste disposal, scale calibration/accuracy, temperature monitoring for A2LA certified labo-

ratory.

EDUCATION MIAMI UNIVERSITY

Oxford, Ohio Bachelors in Microbiology, 2003

Minor in Neuroscience

SKILLS Member of local Toastmasters chapter, serving as role of Treasurer from Jul ’08 - Jun ’09. Required to maintain and

uphold Secret Security Clearance from the U.S. Government. Required to pass thorough background check from De-

partment of Justice. Attended various on-site and off-site training sessions/conferences for bioassay validation and

qualification. Received and completed several immunizations against various bioterrorism agents and pathogenic or-

ganisms. Acute attention to detail and organization. Highly motivated learner with sense of urgency. Can easily adjust

to allow flexibility with assay/study time constraints while simultaneously meeting deadlines. Works well in team envi-

ronment or independently with minimal supervision. Good written and verbal communication skills. Proficient with Mi-

crosoft Word, Excel, and Powerpoint. Familiar with Microsoft Excel VBA.

9596 E LAKE RD, NORTH EAST, PA 16428

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