Medical Human
Resume:
CURRICULUM VITAE
September ****
ADELE L. MILLER
ADDRESS: **** ****** **. *** **
New Brunswick, NJ 08901
aca2gy@r.postjobfree.com
EDUCATION:
****- ****** **********, ** *.S. in Biology (Molecular & Cell
Biology Track)
2004- Salisbury University, MD B.S. in Biology- (Microbiology
Concentration)
EMPLOYMENT:
8/11-present Graduate Assistant, Toxicology Program,
Rutgers University
6/04-8/11 ORISE Intern, USAMRICD
2003 Intern, National Cancer Institute
2001-2004 Technician, Classroom Technology Services
Dept. of Information Technology of Salisbury
University
RESEARCH EXPERIENCE:
2011-present: Graduate Assistant, Toxicology Program, Rutgers
University; Piscataway, NJ. Currently conducting exploratory research
using excised rabbit corneas to understand the changes that occur in
the basement membrane of the cornea following nitrogen mustard
exposure. A main goal is to develop therapeutics against ocular
chemical warfare exposure. Techniques include organ culture,
operation of cryostat, H&E staining and western blotting.
2004-2011: Oak Ridge Institute for Science and Education (ORISE)
Intern at United States Army Medical Research Institute of Chemical
Defense (USAMRICD)-Research Division, Cell and Molecular Biology
Branch; Aberdeen Proving Ground, MD. Design, develop and execute
experiments to support research in the areas of DNA damage, DNA
repair, inflammation, apoptosis and genotoxicity. Research focuses
primarily to elucidate the mechanism of toxicity due to chemical
warfare agent exposure. Various in vitro models were utilized along
with a wide range of biomarkers and techniques. These include primary
cell culture, three dimensional tissue constructs of skin, lung and
eye (MatTek, Lonza), Luminex technology (Bio-Rad software),
fluorescence microscopy (Image-Pro software), comet assay (Loats
software), Meso Scale Discovery system, immunohistochemistry, flow
cytometry, ligand interactions and cell signaling. Responsible for
coordinating and mentoring summer student projects as well as training
new employees, creating SOPs, data analysis, grant writing and general
lab management.
2003: Summer internship with the Protein Engineering Section of the
Macromolecular Crystallography Laboratory at the National Cancer
Institute; Frederick, MD. Research incorporated molecular methods to
identify genes from Y pestis. Techniques include: PCR, random
mutagenesis, recombinational cloning, isolation of plasmid DNA from E.
coli, restriction enzyme digests, agarose gel electrophoresis,
transformation of E. coli with plasmid DNA, bacterial cell culture
methods, SDS-polyacrylamide gel electrophoresis, and DNA sequencing.
2001-2002: Undergraduate Research at Salisbury University;
Salisbury, MD. Studied the genetic diversity between two members of
the genus Desmodium. Co-directed this project which involved
collaboration with Dept. of Natural Resources. Methods include: plant
DNA extraction (Qiagen methods), inter-simple sequence repeats (ISSR),
PCR and gel electrophoresis. Other duties include the training of lab
assistants, current literature collection and ordering of supplies.
ACTIVITIES, AWARDS and TRAINING:
2010 Attendee at the Practical Methods for In Vitro Toxicology
Workshop sponsored by Institute for In Vitro Sciences
2010 Attendee at the Planet XMap Multiplexing Symposium w/ Luminex
XMap University Training
2010 Attendee at the 24th Annual Genotoxicity and Carcinogencity
Workshop sponsored by BioReliance
2009 Invited platform speaker for Society of Toxicology Annual
Meeting
2008 Hands on training of the PorCORA cornea model at MB
Research Labs
2007 Attendee at the ISP/PPP Trailblazing the Skin Frontier Workshop
2005 Attendee at the Cell Culture Techniques Course sponsored by the
BioTechnical Institute of Maryland, Inc.
2004 Deans List; Salisbury University
2002 Salisbury University, Henson School of
Science Undergraduate Research Symposium -Oral presentation
2002 Study Abroad-Coral Reef Biology in Roatan,
Honduras
2001 & 2002 Salisbury University, Henson School Undergraduate
Research Grant Recipient
REFERENCES-Upon request
PUBLICATION:
Gross C, Nealley E, Miller A, Nipwoda M, Smith W. Lack of Genotoxicity in
VX and Soman-Treated Cultured Human Cells by Comet Assay Analysis. J Med
Chem Biol Radiol Def. Nov. 22, 2010. Available at:
http://www.jmedcbr.org/issue_0801/Gross/Gross_11_10.html man cells by comet
assay analysis. man cells by comet assay analysis. J Med Chem Biol Radiol
Def. November 22, 2010. Available at:
http://www.jmedcbr.org/issue_0801/Gross/Gross_11_10.html.
PLATFORM PRESENTATIONS:
2010 Society of Toxicology. Salt Lake City, UT. Different methods to study
the genotoxic marker ?-H2AX following sulfur mustard exposure in cultured
human skin cells and a skin tissue construct. Miller AL, CL Gross, EW
Nealley, OE Clark, NK Waraich, KL Rodgers and WJ Smith.
2010 Biennial Medical Chemical Defense BioScience Review. Hunt Valley, MD.
Sulfur mustard-induced DNA damage and genotoxicity: In vitro studies. Smith
WJ, CL Gross, AL Miller, OE Clark and EW Nealley.
2009 Chemical and Biological Defense Science and Technology Conference.
Dallas, TX. Human bronchial epithelial cells as a model for human lung
airway injury and therapeutic intervention following sulfur mustard
exposure in vitro. Smith WJ, OE Clark, EW Nealley, AL Miller and YS Jung.
POSTER PRESENTATIONS:
2005 Society of Toxicology, New Orleans, LA. Putative role of platelet
activating factor (PAF) analogs in cell cycle aberrations in human
endothelial cells in vitro. Clark OE, EW Nealley, KW Leiter, K Finke, AL
Miller and WJ Smith.
2005 Society of Toxicology, New Orleans, LA. Changes in CD95 expression in
endothelial cells 24 hours following exposure to sulfur mustard or platelet
activating factor. Clark OE, EW Nealley, KW Leiter, K Finke, AL Miller, J
Nicholson and WJ Smith.
2006 Society of Toxicology, San Diego, CA.. Endothelial cell alterations
following in vitro
exposure to sulfur mustard or platelet activation factor. Clark OE, EW
Nealley, KW Leiter, AL
Miller, and WJ Smith.
2006 Biennial Medical Chemical Defense BioScience Review. Hunt Valley, MD.
Changes in CD95 expression in endothelial cells 24 hours following exposure
to sulfur mustard or platelet activating factor. Clark OE, EW Nealley, KW
Leiter, K Finke, AL Miller, J Nicholson and WJ Smith.
2006 Biennial Medical Chemical Defense BioScience Review. Hunt Valley, MD.
Endothelial cell
alterations following exposure to sulfur mustard or platelet activating
factor. Clark OE, EW
Nealley, KW Leiter, AL Miller, and WJ Smith.
2007 Society of Toxicology. Charlotte, NC. Inflammatory cytokine release
in human isolated cell culture and tissue constructs following in vitro
exposure to sulfur mustard. Miller AL, OE Clark, EW Nealley, VL Fuller and
WJ Smith.
2007 Society of Toxicology, Charlotte, NC. The protective effect of
capsaicin analogs on normal
human epidermal keratinocytes (NHEK) following in vitro exposure to sulfur
mustard (SM).
Clark OE, EW Nealley, AL Miller, KW Leiter, and WJ Smith.
2008 Society for In Vitro Biology, Tucson, AZ. Evaluation of cytokine
activity of three human
tissue constructs for in vitro sulfur mustard research. Miller AL, OE
Clark, CL Cario, EW
Nealley, RK Kan and WJ Smith.
2008 Society for In Vitro Biology, Tucson, AZ. The protective effect of
capsaicin analog
treatment on human bronchial cells exposed in culture to sulfur mustard
(HD). Clark OE, EW
Nealley, AL Miller, CL Cario, YS Jung and WJ Smith.
2008 Genetic Toxicology Association. Newark, DE. ?-H2AX formation in three-
dimensional
human tissue models following in vitro exposure to sulfur mustard. Miller
AL, OE Clark, EW
Nealley, KW Leiter and WJ Smith.
2008 Biennial Medical Chemical Defense BioScience Review, Hunt Valley, MD.
Effect of VX on
genotoxicity in cultured human epidermal keratinocytes. Gross CL, EW
Nealley, AL Miller, MT
Nipwoda and WJ Smith.
2008. Biennial Medical Chemical Defense BioScience Review, Hunt Valley, MD
Evaluation of
cytokine activity of three human tissue constructs for in vitro sulfur
mustard research. Miller
AL, OE Clark, CL Cario, EW Nealley, RK Kan and WJ Smith.
2008 Biennial Medical Chemical Defense BioScience Review, Hunt Valley, MD
The protective
effect of capsaicin analog treatment on human bronchial cells exposed in
culture to sulfur
mustard (HD). Clark OE, EW Nealley, AL Miller, CL Cario, YS Jung and WJ
Smith.
.
2009 Society of Toxicology, Baltimore, MD. Evaluation of a capsaicin
analog as a modulator of
inflammatory cytokine release in human tissue models. Clark OE, EW Nealley,
AL Miller, KW
Leiter, YS Jung and WJ Smith.
2009 Society of Toxicology, Baltimore, MD. Inflammatory cytokine response
in EpiDerm FT: A
tissue model for cutaneous SM exposure. Clark OE, EW Nealley, AL Miller,
KW Leiter and WJ
Smith.
2009 Society of Toxicology, Baltimore, MD. ?-H2AX formation in three-
dimensional human
skin and cell models following in vitro exposure to sulfur mustard. Miller
AL, OE Clark, EW
Nealley, KW Leiter and WJ Smith.
2009 Chemical and Biological Defense Science and Technology Conference.
Dallas, TX. Lack of
genotoxicity in cultured small airway cells following exposure to the nerve
agent, VX. Gross,
CL, EW Nealley, AL Miller, MT Nipwoda, OE Clark, and WJ Smith.
2009 Chemical and Biological Defense Science and Technology Conference.
Dallas, TX. Use of cultured human skin cells and a skin tissue construct
to study the genotoxic marker ?-H2AX following sulfur mustard exposure.
Miller AL, CL Gross, EW Nealley, OE Clark, NK Waraich, KL Rogers, and WJ
Smith.
2009 Society for In Vitro Biology. Charleston, SC. Lack of genotoxicity in
cultured human small airway and microvascular cells following exposure to
the nerve agent VX. Gross CL, EW Nealley, AL Miller, MT Nipwoda and WJ
Smith.
2009 Society for In Vitro Biology. Charleston, SC. Use of cultured human
skin cells and a skin tissue construct as models to study the genotoxic
marker ?-H2AX following sulfur mustard exposure. Miller AL, CL Gross, EW
Nealley, OE Clark, NK Waraich, KL Rodgers and WJ Smith.
2009 Society for In Vitro Biology. Charleston, SC. Human bronchial
epithelial cells as a model for human lung airway Injury and therapeutic
intervention following sulfur mustard exposure in vitro. Clark OE, EW
Nealley, AL.Miller, YS. Jung, and W. J. Smith.
2009 Genetic Toxicology Association. Newark, DE Use of cultured human skin
cells and a skin tissue construct as models to study the genotoxic marker ?-
H2AX following sulfur mustard exposure. Miller AL, CL Gross, EW Nealley,
OE Clark, NK Waraich, KL Rodgers and WJ Smith.
2010 Biennial Medical Chemical Defense BioScience Review. Hunt Valley, MD.
Comet analysis of genotoxicity in cultured human cells following exposure
to the nerve agents, VX and GD. Gross CL, EW Nealley, AL Miller, MT
Nipwoda and WJ Smith.
2010 Biennial Medical Chemical Defense BioScience Review. Hunt Valley, MD.
Different methods to study the genotoxic marker ?H2AX following sulfur
mustard exposure in cultured human skin cells and a skin tissue construct., Miller AL, CL Gross, EW Nealley, OE Clark, NK Waraich KL Rodgers and WJ
Smith.
2010 Biennial Medical Chemical Defense BioScience Review. Hunt Valley, MD.
Operation of a cell culture core laboratory to provide human cell and
tissue models used in the study of chemical warfare agents. Nealley EW, MT
Nipwoda, CL Gross, OE Clark, AL Miller and WJ Smith.
2010 Biennial Medical Chemical Defense BioScience Review. Hunt Valley, MD.
In vitro studies of the inflammatory response of human bronchial epithelial
cells to sulfur mustard. Clark OE, EW Nealley, AL Miller, S Russell and WJ
Smith.
2010 Society of Toxicology. Salt Lake City, UT. Comet analysis of
genotoxicity in cultured human cells following exposure to the nerve
agents, VX and GD. Gross CL, EW Nealley, AL Miller, MT Nipwoda and WJ
Smith.
2010 Society of Toxicology. Salt Lake City, UT, Different methods to study
the genotoxic marker ?-H2AX following sulfur mustard exposure in cultured
human skin cells and a skin tissue construct. Miller AL, CL Gross, EW
Nealley, MT Nipwoda, and WJ Smith.
2010 Society for In Vitro Biology. St. Louis, MI. Use of H2AX and
micronuclei formation to evaluate genotoxicity in cultured human skin cells
following sulfur mustard exposure. Miller AL, CL Gross, EW Nealley, OE
Clark, NK Waraich, KL Rodgers, and WJ Smith.
2010 Genetic Toxicology Association. Newark, DE. Use of gamma-H2AX and
micronuclei formation to evaluate genotoxicity in cultured human skin cells
following sulfur mustard exposure. Miller AL, CL Gross, EW Nealley, OE
Clark, NK Waraich, KL Rodgers, and WJ Smith.
2011 Society of Toxicology. Washington, DC. Use of ?-H2AX as a biomarker
to evaluate the anti-genotoxic activity of compounds in cultured human skin
cells following CEES exposure. Miller AL, NK Waraich, CL Gross, EW
Nealley, OE Clark KL Rodgers and WJ Smith.
2011 Society for In Vitro Biology, Raleigh, NC. Effect of Ebselen in
modulating genotoxicity in cultured human cells from chloroethyl ethyl
sulfide exposure using comet assay analysis. Gross CL, EW Nealley, AL
Miller, MT Nipwoda, OE Clark and WJ Smith.
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