Rolf K. Swoboda

*** ********* ****

Upper Darby, PA, 19082, USA

aca17q@r.postjobfree.com

+1-484-***-****

PROFESSIONAL EXPERIENCE - Including Major Achievements and Contributions

The Wistar Institute, Philadelphia, PA, Senior Scientist 1999

to 2013

- Developed a new screening method to clone MHC class II restricted

antigens

- Cloned and characterized several tumor antigens

- Measured the immune response of patients using different immunoassays

(e.g. ELISA, T cell assays [proliferation, cytotoxicity], RT-PCR)

- Established directional RNAseq (NGS) of melanoma and melanocytes to

determine the melanoma specific transcriptome

- Managed the vector database of the group

- In vivo studies

- In vitro cell assays testing putative melanoma treatment targets

- Grant applications and administrative duties (e.g. animal studies,

human studies)

- Supervised students

Louisiana State University Medical Center, New Orleans, LA Scientist

1998 to1999

- Responsible for database connecting patients' information and

experimental results

- Determined the immune response of HIV positive patients to C. albicans

- Point person for the communication between clinicians and bench

researchers of the study

Hans-Kn ll-Institute for Natural Products, Jena, Germany

Junior Research Group Leader 1994 to

1997

- Developed a new screening method to determine genes/proteins involved

in the dimorphism of Candida albicans

- Supervision of students and lab assistants

- Successful grant applications

- Radioactivity safety officer

Blood Donor Service of the Bavarian Red Cross, Wiesentheid, Germany

Substitute Head of the Quality Control Laboratory

Worked as acting representative of the head of the quality control

laboratory

at Blood Donor Service of the Bavarian Red Cross during my graduate

studies.

- Certified that all tested blood products were sterile and endogen-free

- Supervised several groups testing the quality of blood products

- Confirmed that all regulatory requirements were fulfilled for the

preparation of human blood products in a GMP/GLP environment.

EDUCATION AND TRAINING

PhD in Immunology and Molecular Biology - University of W rzburg, Germany

Grade: Sehr gut (Magna cum laude)

Topic: Cloning and characterization of murine Il-2

Diploma (MS) in Biology - University of W rzburg, Germany

Grade: 1.3 (A), emphasis on immunology and molecular biology

Topic: Determination of the optimal B cell stage for productive hybridoma

Human Subjects Protections certificate (CITI collaborative institutional

training initiative)

Responsible Conduct of Research certificate (CITI)

Radiation safety officer certificate

Certificate of Completion of the NIH Research Commercialization course

Approved for experiments with animals

AREAS OF EXPERIENCE

Oncology, especially melanoma; RNAseq; iPS cells; immunology; molecular and

cellular biology; microbiology; mycology

MEMBERSHIPS AND AFFILIATIONS

American Society of Microbiology

STRENGTH AND STYLE

Interpersonal

. Independent, highly motivated performer

. Strong leader, team player, and group facilitator

. Committed, loyal, and hardworking

. Ability to work with all levels and types of people

Professional

. Positive, assertive, and entrepreneurial

. Flexible, adjust quickly to new challenges

. Outstanding decision-making abilities

. Ability to multi-task, respond quickly to new opportunities

. Strong problem solver

. Diplomatic and results oriented

. Excellent written and verbal presentation abilities

. Solid project management, negotiation, and organization skills

. Experienced supervisor for different levels (From high school student

to technician and graduate student)

LANGUAGE PROFICIENCY

. English, German

CITIZENSHIP

. USA

AWARDS AND RECOGNITION

1998 Research grant of the Deutsche Forschungsgemeinschaft (DFG)

1995 Research grant of the state Thuringia

1992 Royal Society research grant

1991 Oral and Dental Research Trust Award 1991/1992, London

1/88 - 12/89 Postdoctoral fellowship of the DAAD (German academic

exchange service).

1985 EMBO travel award

PATENTS

Patent application: US20100303833, "Method for identifying a MHC class

II-dependent tumor associated T helper cell antigen",

Inventors: Herlyn, Somasundaram, Swoboda

TECHNICAL EXPERTISE

. Antibody and hybridoma

. Bead sorting

. Bioinformatics analysis

. Biomarker screening

. Blood typing

. C. albicans manipulations

. Cell transformations and transductions

. Database management

. Dosing (S.C., IV, dermatological, esophageal)

. Electrophoresis

. ELISA

. Flow cytometry (FACS)

. Gene over-expression

. In vivo models (mice, rats)

. In vitro compound testing in cell assays

. iPSC production

. Lentiviral & retroviral vector construction and production

. Mammalian cell and tissue culture techniques

. Maxi, Midi, Mini prep

. Microscopy

. Microsoft Office suite

. PCR/RT-PCR

. Protein isolation and purification

. Experiments with radioactivity

. RNA/DNA isolation

. RNAseq (NGS)

. siRNA and shRNA

. Statistical analysis (using R)

. T cell assays

. Western blotting

PUBLICATIONS

More than 40 publications in peer reviewed journals.

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