Quality Analyst, Research associate
Resume:
Shruthi Nune
**** ******* **, ***** ****, CA ****7
*******.*****@*****.***
Phone: 408-***-****
Career Focus
Diligent biochemist with hands on experience in nucleic acid isolation, purification and sequencing, protein purification techniques. Excellent knowledge of laboratory protocols, quality procedures and analytical instrumentation. Excellent experience in organizing, maintaining and managing laboratory date. Looking for an opportunity to render quality service to the institution. Expertise
Processes & techniques: DNA/RNA isolation, PCR, RT-PCR, Gel electrophoresis, Protein purification, SDS-PAGE gels, Western blotting, Column chromatography, Affinity chromatography and 2D gel electrophoresis. Basic understanding of cell culture techniques. Instrumentation & technologies: Familiar with a range of lab equipment HPLC, GC/MS, UV-Vis, FI-IR spectrometers.
Experience
GRADUATE RESEARCHER
Nov 2015- March 2018
Master’s Thesis: Investigating if division inhibition by the yqgE25::IS1 mutation work through sulA?
• Work and studies centered on E. coli, sulA gene (SOS response gene) and Gene sequencing, amplification using RT-PCR technique.
• Inoculating and growing both wild type (AM620 strain) and mutant cells (AM621) in sterile environment.
• Inducing SOS response in cells by Mitomycin C.
• Extraction, isolation and purification of RNA from the cells.
• Plating the cells on L- Agar plates and checking for phenotypes for any revertants.
• Sequencing using RT-PCR technique and agarose gel electrophoresis. GRADUATE STUDENT ASSISTANT- CSUEB HAYWARD
Apr 2016 – Mar 2017
• Isolation of plasmid pBK-CMV::1.6DNA and electrophoretic analysis of purified DNA.
• Amplification of the SfiD promoter region using Polymerase chain reaction (PCR) and analysis by agarose gel electrophoresis.
• Infrared Analysis of polymer films & liquid mixture by using the Perkin-Elmer FT-IR Spectrometer.
• Ultraviolet/Visible Spectroscopy; Visible Spectra of Permanganate Solutions & UV spectra of RNA molecules using Genesys2 PC UV/Visible spectrophotometer.
• HPLC Analysis for a Mixture of Organic Compounds and RNA-peptide complexes.
• Using Oxford Lab-X3000 X-ray fluorescence (XRF) analyzer to explore the variable parameters in XRF.
• Sample Analysis using various methods offered by GC-MS using an Agilent 7820A GC instrument.
GRADUATE TEACHING ASSOCIATE- CSUEB HAYWARD
Protein Chemistry Techniques.
Jan 2016 - Mar 2016
Monitored students performing experiments such as:
• Partial purification of Lactate dehydrogenase enzyme from Chicken breast by Cell Lysis, High-Speed Centrifugation, Ammonium Sulfate Fractionation, and Dialysis.
• Enzyme assay determination using Nanodrop Spectrophotometer and Bradford Protein assay (binding of Coomassie Brilliant Blue G-250 to purified protein).
• Cibacron Blue-Agarose chromatography- column chromatography to purify dialyzed Lactate Dehydrogenase enzyme using a stationary phase specific for 'Rossmann fold' proteins.
• High-performance Liquid chromatography (HPLC) to separate steroid hormones and discover the optimal solvent mixture.
• Purification of a Maltose Binding protein (MBP)-Paramyosin Fusion protein by Affinity chromatography. SDS-PAGE and Western Blot on purified fusion protein.
• Two-dimensional Gel electrophoresis of protein mixtures. LECTURER IN ENGINEERING, Chemistry
Aug 2009- Feb 2011
SJM Institute of Technology, Karnataka, India.
Taught theoretical as well as laboratory courses of Engineering chemistry. Education
Master of Science, Biochemistry
California State University, East Bay
May 2019
Master of Science, Chemistry
Kuvempu University, Karnataka, India.
June 2009
Bachelor of Science (Physics, Chemistry, Mathematics) Kuvempu University, Karnataka, India
June 2007
Skills
• Experienced in working with nucleic acids and proteins
• DNA/RNA isolation, PCR, End point RT-PCR techniques
• Protein expression and purification techniques
• SDS-PAGE
• 2D Gel electrophoresis
• Proficient in using instruments such as AA, FTIR, HPLC, GC and UV-Vis
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