Medical Animal
Resume:
PETER H. LAPCHAK, Ph.D.
Weymouth, MA 02191
*************@*******.***
www.linkedin.com/in/peterhlapchaklifescientist
ACCOMPLISHMENTS
developed a mouse model to study the immune role of platelets in lupus development.
developed mouse models of platelet depletion/transfusion to study immune responses after injury
in vivo loading of a drug into platelets prevents remote lung injury after mesenteric (intestinal) ischemia/ reperfusion injury
identified that platelets are critical immune modulators that prevent mortality after burn injury in a dose-dependent manner
identified and characterized immune dysfunction in diabetes-prone BB/O rats
EMPLOYMENT/RESEARCH EXPERIENCE & SKILLS
2015-19 Lecturer in Medicine, Harvard Medical School and Director of Animal Research, Rheumatology and Clinical Immunology, Medicine, Beth Israel Deaconess Medical Center. Boston, MA
Studying the immune/inflammatory role of platelets in systemic lupus erythematosus (SLE) in the MRL.lpr and B6.lpr mice using platelet depletion.
- proficient in developing in vivo animal models (autoimmune diabetes, trauma, and SLE) and mouse models of platelet depletion/platelet transfusion.
- used histology, immunocytochemistry, immunofluorescence and confocal microscopy to identify platelets and platelet:leukocyte aggregates in kidneys of mice with disease.
- used multi-parameter flow cytometry to phenotype platelet:leukocyte aggregates, leukocyte subsets, and platelet activation in lupus mice.
- developed of a mouse tumor model to study Ewing sarcoma metastasis.
2007-11 Instructor in Medicine, Rheumatology and Clinical Immunology, Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA.
Developed a research program to study the role platelets in local and remote tissue damage using mouse models of platelet depletion/transfusion. Also trained and directed visiting scientists, research fellows and summer students. I wrote and was the principal investigator on the IACUC animal protocol.
- experience in platelet characterizing immune responses/inflammation in mouse models ischemia/reperfusion injury (I/R I).
- in vivo antibody titering and platelet depletion/platelet transfusion and lymphoid cell passive transfer, mouse surgery.
- experience in drug evaluation in pre-clinical animal models.
- identified differences in platelet responses after local trauma and the importance of signaling pathways in platelet activation in response local trauma; identified their role in remote lung damage after mesenteric I/R injury using histology and immunocytochemistry.
2003-07 Instructor in Surgery, Department of Surgery (Immunology), Brigham and Women’s Hospital and Harvard Medical School, Boston, MA.
Developed a research program to study the role of platelets in the immune response to inflammation. Trained, directed and assisted research fellows.
- experienced in platelet characterization in immune responses in animal models of burn injury.
- in vivo antibody titering for platelet depletion/transfusion and lymphoid cell passive transfer.
- identified differences in platelet responses after burn injury and the role of platelet CD40 and CD154 in platelet activation.
- identified a central role for platelets modulating the pro-inflammatory immune response in preventing mortality after burn injury.
- multi-parameter flow cytometry to phenotype leukocyte/lymphocyte subsets and cytokine production.
1998-99 Research Scientist, Biochemistry/Immunology [New Product Development], Research and Development, Coulter Cellular Therapies, Inc., Medford, MA.
Designed and developed a novel human T lymphocyte expansion platform using proprietary materials for personalized cancer treatment.
1994-98 Post-Doctoral Fellow, Division of Surgical Research and Division of Renal Medicine, University of Massachusetts Medical Center, Worcester, MA.
Studied apoptosis in trauma patient human lymphoid cells and identified potential immunotherapies to prevent diabetes in NOD mice.
EDUCATION
1989-94 Ph.D., Department of Medical Sciences, Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada. THESIS: Dysregulation of Tumor Necrosis Factor in the BB Rat Model of Human Insulin Dependent Diabetes Mellitus.
- characterized the immune system in the BB rat model of diabetes and identified a central role for macrophage dysfunction and tumor necrosis factor-α deficiency in diabetes development
1985-88 M.Sc., Department of Pathology, Faculty of Medicine, McGill University, Montreal, Quebec, Canada. THESIS: Cellular Immune Function in the Diabetes-Prone BB Rat: Effector Mechanisms in Islet Cell Cytotoxicity.
- characterized the immune system in BB rat diabetes; identified reduced helper T cell proliferation and deficient cytotoxic T cell numbers and responses.
1981-83 B.Sc., [Specialization: Cellular and Molecular Biology], Department of Biological Sciences, Faculty of Science, Concordia University, Montreal, Quebec, Canada
SELECTED PUBLICATIONS (https://www.ncbi.nlm.nih.gov/pubmed?term=Lapchak+PH&cmd=DetailsSearch&log$=activity)
Autoimmunity
1. Bialas, AR, J. Presumey, A. Das, C. van der Poel, PH. Lapchak, L. Mesin, G. Victora, GC. Tsokos, C. Mawrin, R. Herbst and MC. Carroll. (2017) Microglia-induced Synapse loss in Type I IFN-mediated Lupus. Nature. Jun 22; 546(7659): 539-543.
2. Prud’homme, GJ., PH. Lapchak, NA. Parfrey, E. Colle, and RD. Guttmann. (1988) Autoimmunity Prone BB Rats Lack Functional Cytotoxic T Lymphocytes. Cell. Immunol. 114(1): 198-208.
3. Lapchak, PH., LJ. Guilbert, and A. Rabinovitch. (1992) Tumour Necrosis Factor Production is Deficient in Diabetes-Prone BB/Wor Rats and can be Corrected by Complete Freund’s Adjuvant: A Possible Immunoregulatory Role of Tumour Necrosis Factor in Prevention of Diabetes. Clin. Immunol. Immunopathol. 65(2): 129-134.
Trauma/Tissue Injury/Inflammation
4. Fujimi, S., MP. MacConmara, AA. Maung, Y. Zang, JA. Mannick, JA. Lederer, and PH. Lapchak. (2006) Platelet depletion in mice increases mortality after thermal injury. Blood 107(11): 4399-4406.
5. Lapchak, PH. L. Kannan, A. Ioannou, P. Rani, P. Karian, JJ. Dalle Lucca, and GC. Tsokos. (2012) Platelets Orchestrate Remote Lung Tissue Damage After Mesenteric Ischemia/ Reperfusion. Am J Physiol Gastrointest Liver Physiol. 302(8): G888-97.
6. Lapchak, PH. A. Ioannou, L. Kannan, P. Rani, J. Dalle Lucca, and G Tsokos. (2012) Platelet CD40 and CD154 Expression is a Requisite for Remote Tissue Damage in C57BL/6J Mice Following Mesenteric Ischemia/Reperfusion. PLoS ONE. 7(2): e32260.
7. Lapchak, PH., L. Kannan, P. Rani, ON. Pumak, A. Ioannou, J. Dalle Lucca, P. Pine, and G Tsokos. (2012) Inhibition of Syk Activity by R788 in Platelets Prevents Remote Lung Tissue Damage after Mesenteric Ischemia/Reperfusion Injury. Am J Physiol Gastrointest Liver Physiol. 302 (12):G1416-G1422.
LABORATORY TECHNIQUES
Animal Breeding/ Handling
Standard breeding for colony expansion; breeding and maintenance of immune compromised mice; backcrossing to introduce a gene on the background of autoimmune mice (MRL.lpr and B6.lpr); passive transfer of cells (T lymphocytes, platelets). SC, IP, ID, IV injections; tumor cell introduction; blood and urine sampling. Induction of specific immune responses with antigen + CFA (Complete Freund’s Adjuvant)
Animal Surgery
Mesenteric ischemia Reperfusion (superior mesenteric artery clamping); suturing
Animal Necropsy
Blood/organ removal; tumor removal; animal perfusion
Biochemical Assays
ELISA; Bradford Protein Assay; Lowry Protein Assay; polyacrylamide gel electrophoresis; Western Blot; 32P-molecular probe labeling and isolation; EMSA (electrophoretic mobility shift assays) and supershift assays
Cell Assays
T lymphocyte proliferation (H3-Thymidine incorporation); MLR (mixed lymphocyte reaction; CMC (51Cr-release cell-mediated cytotoxicity)
Cell Culture
Cell culture of primary endothelial cells, lymphocytes, macrophages; tumor cell lines
Cell Isolation
Nylon wool purification of T lymphocytes; MACS (magnetic-activated cell sorting): positive and negative selection of specific immune cell populations; centrifugal purification of mouse platelets from whole blood.
Flow Cytometry
Single cell preparation; multi-parameter immune cell phenotyping; quantification of intracellular cytokines; platelet:leukocyte aggregate determination; apoptosis
Histology/Tissue
Organ/tissue removal, processing, embedding; cutting; mounting and staining (H&E); immunochistochemistry; Frozen tissue processing, OCT embedding, cutting, mounting, staining, immunohistochemistry.
Microbiology
Bacterial cultures (plate and liquid); colony isolation and purification; plaque assays
Microscopy
Light microscopy, fluorescence microscopy
Molecular Biology
RNA isolation/purification, gel shift supershift assays, cytoplasmic/nuclear lysate preparation, western blotting ELISA, PCR/RT-PCR; genotyping
Other Animal Work
Immune studies (T cells, macrophages, cytokines) with diabetes-prone rats
Other Lab Skills
Confocal microscopy
Other Skills
Software
Microsoft Office; Graphpad Prism; Kaluza Analysis; FlowJo; EndNote; Adobe Photoshop
Management and Mentoring
Managed research project; directed and guided research fellows, visiting scientists and technicians; collaborated with other scientists on research projects and as an expert research consultant; trained new personnel for animal work; guided and assisted junior lab members with animal work
Writing
Wrote scientific abstracts, manuscripts, grants, annual and technical reports, review and edit abstract, manuscript and grant applications, animal protocols/annual renewals/amendments/new personnel submissions, and Committee on Microbiological Safety (COMS) submissions/annual and three year renewals
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