Manager Technical

Lualhati Eroles-Harkins, M.S. QIHC

EDUCATION

Masters of Science, Mississippi State University, Mississippi State, MS. 39762. (1983) Applied

Biology (thesis research in Entomology.).

Thesis: Biology and Life History of Clearwing Borer, Paranther dollii in Nurseries in North

Mississippi

Bachelor of Science, University of the Philippines at Los Banos, Laguna, Philippines. (1977)

Agriculture. Major: Entomology.

Thesis: Insecticidal Activity of Marigold, Tagetes spp.

CAREER RELATED WORK EXPERIENCE

11/88 to August 30, 2018: Department of Veterans,Affairs, Pathology and Laboratory Medicine, 700 south, 19th street, Birmingham, Ala. 35233.

SUMMARY OF DUTIES : Histotechnologist / Immunohistochemistry

Specialist

Evaluates the validity of results obtained in relation to the test system and collection

procedures. Interprets and correlates staining results with established staining criteria.

Creates uses or modifies logic to determine corrective action for procedural/staining

problems.

Documents all discrepancies, modifications, and corrective actions in appropriate reports.

Accurately prepares stores, maintains, validate reactivities and orders inventory of

antibodies, reagents and supplies for all Immunohistochemical (IHC), staining procedures.

Collects harvests and processes all control material used in immunohistochemistry

Creates, maintains and revises Standard Operating Procedures in the IHC Procedure Manual

annually as directed.

Performs technical aspects to prepare tissues and body fluids for diagnostic evaluation. This

may include routine embedding, microtomy, staining, routine laboratory equipment

maintenance, special staining techniques, immunohistochemistry, and enzyme histochemistry.

Must ensure all regulations are met and all productivity and quality standards are met or

surpassed.

Assists the Pathologist in performing gross analysis of tissue prior to slide preparation.Uses

microtomes, histological processors, slide staining equipment, and automated immunostainers

equipment. Computer skills necessary entry of data entry and patient information access.

Train on regular basis on biohazard, fire and other safety procedures, and other laboratory

required .

01/2019 to present : Senior Bioengineer ( Consultant ) INCYSUS Therapeutics, Inc, 79

Madison Avenue,New York, New York 1006. Local address: : Innovation Depot Inc.,1500 1 st

Avenue North, 35203. Duties Include : Histology, Immunohistochemistry, Microscooy and Imaging and other molecular techniques and scientific support for Gamma delta cell therapy studies .

• Provide technical expertise in immunohistochemistry, microscopy and other molecular tissue techniques to support gamma delta T Cell Therapy studies. • Develop and optimize all methodologies to allow procurement of high-quality data for projects involving gamma delta T cells. • Develop protocols to allow very sensitive detection relevant to proteomics and genomics. • Provide highest quality images for publication, clinical trials as well as project proposals and renewals. • Evaluate the validity of results obtained in relation to the test system and collection procedures. • Interpret and correlate staining results with established staining criteria. • Create, use and/or modify logic to determine corrective action for procedural/staining problems. • Document all discrepancies, modifications, and corrective actions in appropriate reports. • Accurately prepare, store, maintain, validate reactivities and order inventory of antibodies, reagents and supplies for all Immunohistochemical (IHC) staining procedures. • Collect, harvest and process all control material used in immunohistochemistry

11/2005 – 4/2006: Consultant Biologist, Department of Transplantation Immunology,

University of Alabama in Birmingham, Birmingham, Alabama

Technical Duties

1) Develop, improve and design technical protocols for all methodologies necessary for all

projects involved.

2) Supervise student assistants, post doctorate and technologists seeking access to

technologies from the center of immunohistochemistry and related technologies.

3) Contact collaborators and set up experiment models and provide technical expertise

andsuggestions.

4) Prepare budget for supplies and personnel and issue invoices on regular basis for users

of facility.

5) Initiate, maintain contact with diagnostic and reagent companies for collaborations

And demonstration of new and recent reagents, equipment as well as beta and site testing. 6)

Maintain and update requirements for certification and permit for laboratory.

1/2002-08/30/2018 Technical Manager and Owner, Immuno Consultants,

Birmingham, Alabama

Primary duties: Provide technical expertise to researchers identified with needs for highly

specialized methodologies in immunohistochemistry and other molecular technologies. Develop

and optimize all methodologies to allow procurement of high quality data for projects involving

tumor biology, transplantion biology and dynamics of virology.

Develop protocols to allow very sensitive detection relevant to proteonomics and genomics.

Provide highest quality images for publication, clinical trials as well as project proposals and

renewals.

3/2005-11/2006: Research Consultant/Collaborator- Department of Cellular and Molecular

Immunology, Karolinska Institute University Hospital, Stockholm, Sweden

10/2004 to 11/ 2005: Research Consultant Collaborator- Department of Molecular

Epidemiology, MD Anderson Cancer Center, Houston, Texas

11/99-4/2004: Research Consultant/Biologist- Department of Surgery/Neurosurgery Division,

University of Alabama in Birmingham

1) Procure and identify frozen and paraffin sections of human tumors for studies on

Localization of virus protein and genes in human brain tumor.

2) Conduct imaging methods, such as routine light microscopy, Immuno-Electron

Microscopy, Multiple label Flourescence microscopy and confocal microscopy.

3) Isolate, extract, purify rna, dna from tissue culture, frozen and paraffin sections, and

archived tissues from human and animal tissues (normal and tumor).

4) Coordinate, and perform Polymerase Chain reactions, Laser Capture microdissection,

prepare samples for gene sequencing, and other invitro assays in relation to project involving

Localization and identification of viruses and its association to human tumor biology.

5) Maintain all equipments, prepare orders for supplies, reagents and equipments related to

all the experiments.

6) Organize all stained slides for confirmation of interpretation by pathologists. Organize

andcorrelate all the data obtained from invitro studies and imaging results for publication.

11/87-11/88: Associate Scientist (Biochemistry-Cell Biology)-Southern Research Institute.

Birmingham, Alabama

Duties include: Supervise, Coordinate and provide technical expertise electron microscopy for

the entire Molecular Biology Department

8/87 -12/87: Research Assistant III - Department of Oral Biology and Physiology, Medical

College of Georgia.

Duties include: Coordinate analysis and related technologies for fluoride levels and toxicity

both on humans and animals.

4/85 - 8/87: Biological Laboratory Technician (Electron Microscopy and Histology) - Veterans

Administration Medical Center. Medical Research Division Augusta, GA.

Duties include: Supervise, Coordinate and provide technical expertise electron microscopy for

the entire Research department

11/84-3/87: Electron Microscopy Technician III - Department of Ophthalmology, Medical

College of Georgia, Augusta, Georgia

Duties include: Supervise, Coordinate and provide technical expertise electron microscopy for

the entire Opthalmology Department

TEACHING EXPERIENCE

9/82 -12/82, 7/78 - 11/78 Laboratory Instructor - Department of Biology, University of Alabama

in Birmingham, Birmingham, Alabama

Immuno Histochemistry and Molecular Biology Technologies Instructor and Trainer –

1991 to present (Please see details on Courses and Workshops conducted)

HONORS AND AWARDS

Excellence in DNA In Situ Hybridization, 2005, National Society of Histotechnology

Excellence in DNA In Situ Hybridization, 2002, National Society of Histotechnology

President’s Award for Service Excellence, 2003, Alabama Society of Histotechnology

Governor’s Award, State of Alabama for Outstanding Contribution to Health Sciences, 2003

International Education Award, 1979. Rotary International Educational Award National

Science Development Board Graduate Fellowship, 1978.

Best Research Paper Award, National Science Development Board, Philippines, 1979.

PATENTS

Detection Format for Low Copies of Human Cytomegalovirus on Major Human Tumors:

CoInventor.2004

Stabilizing Reagent for Frozen Sections FrozFix@: Primary Inventor, 2006

PROFESSIONAL LICENSE

Immunohistochemistry Qualification, ASCP, 1996 to date

Professional Entomologist. 1983-1987

CONSULTANT DUTIES:

August 3, 2010 to August 10, 2010 – Consultant to Dept of Vaccine Development, Royal

Brisbane Hospital, Brisbane, Australia

November 2006 to March 2007-Consultant to Juvenile Diabetes and Transplantation Proj.

Department of Surgery, Transplantation Immunology Section, University of Alabama in

Birmingham, Birmingham, Alabama

March 2006 to April 2007: Consultant to University of Cincinnati, Pediatric Center, Cincinnati,

Ohio. Provide very specialized expertise on detection and expression of Stem Cells on bone

marrow derived studies.

March2006 to November 2007: Consultant to Karolinska Institute, Molecular Immunology

Department, Stockholm, Sweden

Provide technical support and expertise on ultrasensitive detection of human viruses on human

tumors. Provide active support in development of methodologies involved in proteonomic and

genomic studies.

November 2005 to November 2007: Consultant to MD Anderson Cancer Center, Department

of Immunology, Houston, Texas

Provide technical support and expertise on ultrasensitive detection of human viruses on human

tumors. Provide active support in development of methodologies involve in proteonomic and

genomic studies.

April 2004 to date: Consultant to Department of Neurosurgery. Neurosurgery Research.

California Pacific HealthCare, San Francisco California.

Provide high level expertise on methodologies for ultrasensitive detection of human viruses in

human brain tumors.

Jan 1999- April 2004. Consultant to Brain Spore Program. Department of Surgery,

Neurosurgery Division, University of Alabama in Birmingham, Birmingham, Alabama

Provide high level expertise on methodologies for ultrasensitive detection of human

viruses in human brain tumors.

February 2009- present. Research consultant. Cancer Center, University Alabama in

Birmingham. Provide technical expertise in molecular methodologies for researches in

pediatric hematology. Develop novel methologies for Immunohistochemistry and DNA In situ

hybridization

CUSTOMER SERVICE EXPERIENCE

09/ 2007 TO 02/2015 : Work Include custom support specialist, store associate, Merchandise

specialist to retail companies such as Chicos Fashion, Ann Taylor stores, Hancock Fabrics and

Sears Holdings Inc.

REFERENCES:

Available upon request

VOLUNTEER WORK

Technical Resource: Provide objective and unbiased technical hints on immunohistochemistry

methods and troubleshooting techniques to colleagues needing help through

Immunohistochemistry Resource Group. (2002 to present)

Science Fair Coach: Provide hands on Technical training on Science and Technology for 6 th -8 th

Grade Science Olympiad Team, Our Lady of Valley School

Birmingham, Alabama (2001-2003)

Technique Advisor: Provide hands on training for investigators needing expertise on custom

protocols for Immunohistochemsitry and DNA In situ Hybridization and other Techniques.

Department of Nutrition, Pediatric Infectious Diseases, Department of Surgery, Department of

Hematology and Immunology, University of Alabama in Birmingham. (1999-2004)

Career Day Speaker- Public and Private Schools Elementary and Highs School), Birmingham,

Alabama (1995-2002)

Polynesian Dances and Multicultural Activities Ambassador: Coordinate and actively perform to

provide entertainment to Nursing Home Facilities In Birmingham Alabama., Active member of

Mississippi State University Annual International Fiesta and Performer for Starkville, Mississippi

Annual Cotton District Arts Festival (1999- present)

Polynesian Dances Workshop. Discover The world of Polynesia Starkville, Mississippi Boy

Scout Association, Blue and gold Awards Banquet. February 5, 2007

Diversity Manager, Asian Pacific Group. Birmingham Veterans Hospital. 2003-2011 coordinate

group activities for annual performance in celebration of Asian- Pacific week

Scientific Peer reviewed publications: Available upon request.

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