Tzuu-Wang Chang (Alex Chang), PhD

Randolph, MA **368

Tel: 508-***-**** (cell)

Email: ac80ki@r.postjobfree.com

Current Visa Status: USA Citizen

My Personal GitHub: https://github.com/alextwc/

O BJECTIVE

Seeking for the following related positions:

Bioinformatician, Biostatistician, Software Engineer, Data Analyst, Research Scientist, Post-Doctoral Fellow, Information System Engineer/Manager/Administrator, Network Administrator, Database Administrator EDUCATION

Highest Degree: Ph.D (2006~2011)

Post-Doctoral training at MGH, UMASS/GeneTherapyCenter and BWH/ChanningLab, MA. USA.

Ph.D in Biomedical Engineering & Biotechnology, University of Massachusetts, MA. USA.

MS in Biomedical Engineering & Biotechnology, University of Massachusetts, MA. USA.

MS in Computer & Information Science, University of Massachusetts, MA. USA.

MS in Molecular Genetics, National Yang-Ming Medical University, Taiwan.

BS in Life Science, Fu-Jen Catholic University, Taiwan. PROFESSIONAL EXPERIENCE

(Selected contracts & employments have included)

Freelancer and manuscript/paper reviewer, Taiwan Mar 2017 – Now I traveled back to Taiwan since March of 2017 for an emergent family issue and stayed at Taiwan to look after my father who was in end-stage cancer status and escorted him to do kidney dialysis every two days till he passed away. My father died peacefully at last August and I came back the USA around late November after I completed three months of period of mourning according to the traditional culture. During the interval in Taiwan I spent most of my time in caring my father therefore I can only work as a part time manuscript and paper reviewer to keep my studies in Public Health, Translational Medicine, Genomic Science and Bioinformatics and other related fields. My employers are my past colleagues from Taipei Veterans General Hospital, National Yang-Ming University and National Health Research Institute of Taiwan Government. Channing Division Network Medicine & Department of Cardiac Anesthesiology of BWH, Boston, MA Dec 2013 – Dec 2016

Position Title: Research Fellow

Job property: Post-Doctoral training

Job contents: Dry lab analytic work only (for team work & independent projects without subordinates)

Finished “Transcribe_CABG” project which is a cardiac genomics project for comparing the association study outcomes of patients before and after coronary artery bypass graft surgery. The goal is hopefully to identify the functional cis- and trans- elements triggered by ischemia or other surgical conditions. I developed the RNA-Seq QC & Mapping pipline to get the normalized expression dataset to be used to combine our imputed genotyping dataset to finally perform the eQTL and GWAS Catalog co-localization studies to obtain the functional SNP candidates and eQTL gene candidates and compared them with GTEx cardiovascular data. I also anlyzed the differential gene expression between baseline and ischemia states using DESeq2 and HTSeq packages. I did the PEER factor analysis (Probabilistic Estimation of Expression Residuals) to regress out both known and hidden covariates to increase the statistical power and the eGene quantity of our states-specific and response-specific cis-eQTL analyses.

The coding skills and techniques I have developed and learned from this project including the following tools and software packages: RStudio shiny web, PLINK, Impute2, PEER, GSEA, GATK, IGViewer and SNPsnap of Broad Institute, CytoScape, MatrixEQTL, gQTLstats and gQTLBase from Bioconductor etc.

Published papers related to the above mentioned skills and accumulated techniques:

(01) “The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle CLINICAL PERSPECTIVE” Louis A Saddic, Martin I Sigurdsson, Tzuu-Wang Chang, Erica Mazaika, Mahyar Heydarpour, Stanton K Shernan, Christine E Seidman, Jon G Seidman, Sary F Aranki, Simon C Body, Jochen D Muehlschlegel (2017). “Circulation: Genomic and Precision Medicine” 10(1),e001534. PMID: 28115490

(02) “Post-operative atrial fibrillation examined using whole-genome RNA sequencing in human left atrial tissue.” Martin I Sigurdsson, Louis Saddic, Mahyar Heydarpour, Tzuu-Wang Chang, Prem Shekar, Sary Aranki, Gregory S Couper, Stanton K Shernan, Jochen D Muehlschlegel, Simon C Body (2017). “BMC Medical Genomics” 10(1):25. PMID: 28464817

(03) "Duration of postoperative atrial fibrillation after cardiac surgery is associated with worsened long-term survival." Sigurdsson, Martin I, Nicholas T Longford, Mahyar Heydarpour, Louis Saddic, Tzuu-Wang Chang, Amanda A Fox, Charles D Collard, Sary Aranki, Prem Shekar and Stanton K Shernan (2016). "The Annals of Thoracic Surgery" 102(6):2018-2026. PMID: 27424470

(04) “Allele-specific expression in the human heart and its application to postoperative atrial fibrillation and myocardial ischemia.” Martin I Sigurdsson, Louis Saddic, Mahyar Heydarpour, Tzuu-Wang Chang, Prem Shekar, Sary Aranki, Gregory S Couper, Stanton K Shernan, Jon G Seidman, Simon C Body, Jochen D Muehlschlegel (2016). “Genome Medicine” 8(1),127. PMID: 27923400

(05) "Integrated microRNA and mRNA responses to acute human left ventricular ischemia." Saddic LA, Chang TW, Sigurdsson MI, Heydarpour M, Raby BA, Shernan SK, Aranki SF, Body SC, Muehlschlegel JD.

(2015). "Physiol Genomics." 47(10): 455-462. PMID: 26175501

(06) "Gene Expression and Expression Quantifying Trait Loci Analysis of Left Atrium of Patients With Post- operative Atrial Fibrillation." Sigurdsson, Martin I, Mahyar Heydarpour, Louis Saddic, Tzuu-Wang Chang, Stanton K Shernan, Prem Shekar, Sary Aranki, Gregory Couper, Jochen D Muehlschlegel and Simon C Body (2015). "Circulation" 132(Suppl 3): A12905.

(07) "Identification of novel candidate risk genes for atrial fibrillation after cardiac surgery using high- throughput RNA sequencing of human left atrium tissue:o15–07." Sigurdsson, Martin Ingi, Mahyar Heydarpour, Louis Saddic, Tzuu-Wang Chang, Stanton K Shernan, Jochen Danny Muehlschlegel and Simon C Body (2015). “ACTA ANAESTHESIOLOGICA SCANDINAVICA 59,43” WILEY-BLACKWELL 111 RIVER ST, HOBOKEN 07030-5774, NJ USA.

Gene Therapy Center under RNA Therapeutics Institute, UMASS Medical School, Worcester, MA Feb 2013 – Dec 2013

Position Title: Post-Doctoral Research Associate

Job property: Post-Doctoral training

Job contents: Including both wet lab bench work and dry lab analytic/computing/coding work

(for team work & independent projects with subordinates from temporal summer students)

Finished the Deep Sequencing Data Analyses and Differential Gene Expressions (Cuffdiff & cummeRbund) among several miRNAs knockdown mouse-groups to study the “functional genomics of miRNAs”, “Long-term Oncogenic effects of gene therapies mediated by recombinant adeno-associated virus (rAAV)” and the “Therapeutic Efficacy of gene therapies through the combinations of AAV+miRNAs”. Independently finished the construction and installation for both hardware and software platforms. (A dedicated and customized Linux Server has been built/assembled to compare the computation efficiency with the High Performance Computing Cluster.)

Completed the site-directed mutagenesis (for AAV2 & AAV5) on a special phenotype-specific (Blood Brain Barrier transportable) sequence variation site which was discovered by my structural alignment analyses.

Finished designing the “Quantizing Biological Tissue Tropism of recombinant Adeno-Associated Viruses using ITC measured Thermodynamic Dataset”.

The bench work skills and techniques I have learned and accumulated from this stage: AAV vector sequences analyses and designing, computer 3D alignment of AAV vector protein structures, AAV vector site-directed mutagenesis, qPCR, flow cytometry, eukaryotic and prokaryotic cell cultures, isothermal titration calorimetry, NTI-Invitrogen-Vector, molecular cloning. Cardiovascular Research Center of Mass. General Hospital, Boston, MA Jan 2012 – Dec 2012

Position Title: Research Fellow

Job property: Post-Doctoral training

Job contents: Dry lab analytic work only (for independent project without subordinates)

Finished the Data & Statistics Analyses of Clinical project: Cardiovascular & Metabolic Patient Cohort Project (funded by Pfizer). It was a 5 years Prospective Cohort Study and I was recruited to join the team at its 4th year (2012). Pfizer closed the project when the conttract was finished at the end of 2012 therefore I can only focus on analyzing their baseline clinical data (most completed) to turn out to be a

“Cross-Sectional Study”.

Completed many statistical tests/analyses by using ANCOVA, Cluster Analyses, Linear Regression, Multivariate Analyses, Factorial Variance Analyses.

The analytical work skills and techniques I have learned and accumulated from this stage: Clinical cohort study designs and analyses, SAS 9.0/SPSS 20, Minitab, GraphPad Prism, StudyTrax. Botulinum Research Center at University of Massachusetts, N. Dartmouth, MA Aug 2006 – Sep 2011

Position Title: Research & Teaching Assistant

Job property: PhD student training

Job contents: 3 4 wet lab bench work and 1 4 dry lab data analyses

(for both team work and independent projects with subordinates from junior graduate students and undergraduate students)

Finished the following projects: Aptamer Project (developed 7 inhibitory RNA aptamers against botulinum neurotoxin A), SNAG & SNAGER Project (developed full-length FRET substrates, red-green couple), BoNT/F-LC cloning Projects (Cloned & Expressed recombinant botulinum neurotoxin type F light chain), High-Band identification and Sequence Analyses of Botulinum Neurotoxin Complex

(Isolation & Purification of Botulinum Neurotoxin A complex and N-terminal amino acid sequencing for unknown protein band then confirmed its identity from protein sequence database), Inhibitors kinetics (to identify the inhibition kinetics of botulinum neurotoxin inhibitors), Testing of Bliss Independence (for drug synergy).

Completed many important data analyses by using inferential statistics and comptuer software packages.

( Please click my PhD dissertation link to understand more details on my skills: https://drive.google.com/open?id=1x2Lkni3IWKUq4TBjvMX49P63P_O-8aZ4 )

Most of my bench work skills and wet lab experiences were established during this stage including the fields of Protein Chemistry (Isolation and purification of Recombinant Proteins, structures and functional relatioship analyses, fluorescence labeling and in vivo ex vivo assay, enzyme kinetics etc), Nucleic Acid Chemistry (DNA & RNA preparation, isolation, purification and sequencing, PCR amplification and in vitro transcription of RNA aptamer etc), Analytical Chemistry (Spectroscopy, Electrophoresis, Chromatography, Biacore Surface Plasmon Resonance, Mass Spectrum etc), Molecular Biology

(Molecular Cloning strategies and primer sequences designing, site-directed mutagenesis, kimeric and recombinant protein cloning and designing etc) and prokarytotic cell cultures (competent cell preparation propagation and plasmid transformation, aseptic operation technique etc).

The above mentioned skills and accumulated lab techniques were proved by these published papers:

(01) "Selection of RNA Aptamers Against Botulinum Neurotoxin Type A Light Chain Through a Non- Radioactive Approach." Chang Tzuu-Wang, Pavithra Janardhanan, Charlene M Mello, Bal Ram Singh and Shuowei Cai (2016). "Applied biochemistry and biotechnology" 180(1):10-25. PMID: 27085355

(02) “In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxin”. Chang TW, Blank M, Janardhanan P, Singh BR, Mello C, Blind M, Cai S. “Biochem Biophys Res Commun”. 2010 Jun 11;396(4):854-860. Epub 2010 May 7. PMID: 20452328

(03) "Evolutionary Traits of Toxins." Kumar, Raj, Tzuu-Wang Chang and Bal Ram Singh (2015). "Biological Toxins and Bioterrorism": 527-557.

(04) "The botulinum neurotoxin complex and the role of ancillary proteins."Singh, Bal Ram, Tzuu-Wang Chang, Roshan Kukreja and Shuowei Cai (2014). Molecular Aspects of Botulinum Neurotoxin, Springer New York: 69-101.

(05) Immunological characterization of the subunits of type A botulinum neurotoxin and different components of its associated proteins. Kukreja R, Chang TW, Cai S, Lindo P, Riding S, Zhou Y, Ravichandran E, Singh BR. “Toxicon”. 2009 May;53(6):616-624. PMID: 19673075

(06) Expression, purification and comparative characterisation of enzymatically deactivated recombinant botulinum neurotoxin type A. Weiping Yang, Paul Lindo, Stephen Riding, Tzuu-Wang Chang, Shuowei Cai, Thuan Van, Roshan Kukreja, Yu Zhou, Kruti Vasa, Bal Ram Singh. “The Botulinum Journal (TBJ)”. 2008 Volume 1 - Issue #2:219-241. DOI: 10.1504/TBJ.2008.026477

(07) Horizontal gene transfer may involve Clostridium botulinum neurotoxin evolution. TW Chang, BR Singh. FASEB Journal 2007 Apr;21(5), A622-A622

(08) Botulinum neurotoxin light chain refolds at endosomal pH for its translocation. Cai S, Kukreja R, Shoesmith S, Chang TW, Singh BR. “Protein J.”. 2006 Dec;25(7-8):455-462. PMID: 17115255

(09) "Nucleotide and protein sequence analyses reveal role of neurotoxin associated proteins in food poisoning potential of Clostridium botulinum." Chang TW and BR Singh (2004) May:18(8), C244-C244. FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL 9650 ROCKVILLE PIKE, BETHESDA, MD 20814- 3998 USA.

(10) Analysis of the Drosophila Gene for the Laminin B1 Chain. Ch Gow, Huiyun Chang, Cj Lih, TW Chang, Chofat Hui. “DNA and Cell Biology”. 1993 Sep;12(7), 573-587. PMID: 8397815 F UNCTIONAL S UMMARY

(Problem solving & important credits are listed as follows)

Developing RNA-Seq, ChIP-Seq, DESeq2, HTSeq and other sequence mapping, assembling and quantitating pipelines, performing genotyping imputation, GWAS, eQTL, GSEA, ORA, WGCNA and other bioinformatics data analyses with UNIX shell scripting and python + R programming (Bioconductor packages, iPython, Jupyter Notebook) in customized LINUX clusters or “Cumulus Clouding” environments at Gene Therapy Center of UMASSMED, Channing Cluster of BWH and Orchestra Cluster of HMS as a bioinformatician and a biostatistician during 2013~2016.

Working as a biostatistician for Cardiovascular & Metabolic Patient (CAMP) Cohort Study at MGH

(Massachusetts General Hospital Cardiology Division / Harvard Medical School) during 2012.

Designed the customized and modified SELEX protocol to harvest 7 RNA aptamers under low radioactive risk, low cost, high yield, better purity and shorter cycle timing for botulinum neurotoxin antidote project during 2007~2011.

Completed the FRET (Fluorescence Resonance Energy Transfer) substrate designing and construction by conjugating a couple of red fluorescent protein and green fluorescent protein to the flanking sites of a full length of SNAP-25 recombinant protein to develop a high sensitive biosensor system for detecting botulinum neurotoxin activity potentially existed in contaminated food specimens during 2006~2008.

Solved the tough cloning issues and problems of translocation domain existed on Botulinum Neurotoxin type F Heavy Chain and successfully obtained the individual clones of BoNT/F leight chain and heavy chain during 2003~2007.

Solved the cloning problems and proposed the decisive cloning strategy to successfully create the double-mutant E224A/E262A full-length recombinant botulinum neurotoxin Type A to be used as a neurotoxin surrogate for neurotoxin kinetics research, vaccine preparation and USA patent application of UMASS during 2003~2007.

Independently completed the Web/Database Project, “Chinese Gene Variation Database (CGVD)” for National Health Research Institute (NHRI) of Taiwan Government. (Funded by National Science Foundation of Taiwan). http://www.cgvdb.tw/ (PHP+MySQL coding project) during 1998~2000.

Well-trained Mathematics (linear algebra, discrete math, probability theorem, inferential statistics), Physics (Classic Mechanics, Physical Chemistry, Thermodynamics, Fluid Dynamics, Electromagnetism and Quantum Mechanics) and Information Science (AI and machine learning) foundations.

1 year experience in recombinant Adeno-associated Virus mediated Gene Therapy (Episomal Gene Therapy) with analyzing the deep sequencing data (RNA-seq) and vector designs.

3 years working experiences in Computational Genomics and Statistical Genetics with patients NGS datasets, SNP imputation dataset and patient phenotype datasets.

3 years working experiences in Bioinformatics (Nucleic Acid and Protein sequence analyses) and cheminformatics (structure folding, protein docking and computer simulations).

7+ years working experiences in Biochemistry & BioSafetyLevel-3 Lab for Protein Chemistry, Molecular Cloning, Toxicology, bacteria & mouse cell cultures, and other related projects.

Personal Expertise span across four professional domains: (1) “Mol. Biology & Cloning” domain (2)

“Protein Chemistry & Biophysics” domain (3) “Information Technology” related domain (4) “Bio-statistics”.

2 years of administrator and manager experience in hospital management and logistical supplies while working as a medical officer (lieutenant) during serving a Field Hospital of Taiwan's Army. Also have one semester of Teaching Assistant experience in Freshman Chemistry lab. PERSONAL FEATURES

Interdisciplinary Background:

A good mediator between dry lab and wet lab and a good jargon translator between biological sciences and stochastic sciences

Multiple lingual background (Chinese-Mandarin, Chinese-Taiwanese, Japanese and English): A good translator on those science journals/articles published by Chinese or other eastern Asian languages

International background (dual citizenship owner of USA and Taiwan): It will be highly useful for those companies or institutions that have important business/research activities/collaborations in China, Hong-Kong, Singapore or Taiwan. TECHNICAL SKILLS

M OL. B IOLOGY & B IOCHEMISTRY FIELD:

Lab Techniques: Spectroscopy (UV/VIS, CD, IR), Electrophoresis (PAGE, Agarose), Chromatography (Gel Filtration, Affinity, Ion Exchange, HPLC), PCR (qPCR, RT-PCR, Two-Staged PCR), ImmunoBlot Assay (Western Blot, Dot Blot, sandwich ELISA), Culture (Bacteria, Eukaryotic Cell), Recombinant Protein Preparation & Purifications, Molecular (TOPO) Cloning & Vectors Constructions, Combinatorial Chemistry (SELEX), Enzyme Inhibition & Kinetics, FRET

(Fluorescence Resonance Energy Transfer) applications (substrate construction), Isothermal Titration Calorimetry (ITC).

Software: MS Office, Origin Pro, MatLab, Mathematica 7, SAS University Edition/SAS studio, SPSS 20, Minitab, GraphPad Prism, R-Studio, VMD, Autodock, MeDock, RasMol, PyMol, Chimera, DeepView, EndNote, Bowtie, Tophat, Crossbow, Myrna, Cufflink, STAR (Spliced Transcripts Alignments Reconstruction), FastQC, GCG (Genetics Computer Group) Packages, Vector NTI, CummeRbund, iFoldRNA, Bioconductor, UCSC Genome Browser, Animated GIF.

C OMPUTER & P ROGRAMMING FIELD:

Operating Systems: Unix System V, Linux/Ubuntu, FreeBSD, RedHat/CentOS, Mac, Windows Coding Languages: C, JAVA, Python, Tcl/Tk, Perl, PHP, HTML/XML, SQL, BASH Shell Scripting Networking: TCP/IP, FTP, DNS, Apache, SSH/Telnet

Relational DBMS: DBmaker, MySQL, Oracle, MS SQL server Unix Tools: GCC, Vim, Xming, WinSCP, Putty, Bourne Again Shell (Bash) Scripting etc. Others: CGI scripting, DreamWeaver, Flash, VMware, UltraEdit, Eclipse-SDK.4.2.2. P UBLICATIONS

(01) “The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle CLINICAL PERSPECTIVE” Louis A Saddic, Martin I Sigurdsson, Tzuu-Wang Chang, Erica Mazaika, Mahyar Heydarpour, Stanton K Shernan, Christine E Seidman, Jon G Seidman, Sary F Aranki, Simon C Body, Jochen D Muehlschlegel (2017). “Circulation: Genomic and Precision Medicine” 10(1),e001534. PMID: 28115490

(02) “Post-operative atrial fibrillation examined using whole-genome RNA sequencing in human left atrial tissue.” Martin I Sigurdsson, Louis Saddic, Mahyar Heydarpour, Tzuu-Wang Chang, Prem Shekar, Sary Aranki, Gregory S Couper, Stanton K Shernan, Jochen D Muehlschlegel, Simon C Body (2017). “BMC Medical Genomics” 10(1):25. PMID: 28464817

(03) "Duration of postoperative atrial fibrillation after cardiac surgery is associated with worsened long-term survival." Sigurdsson, Martin I, Nicholas T Longford, Mahyar Heydarpour, Louis Saddic, Tzuu-Wang Chang, Amanda A Fox, Charles D Collard, Sary Aranki, Prem Shekar and Stanton K Shernan (2016). "The Annals of Thoracic Surgery" 102(6):2018-2026. PMID: 27424470

(04) “Allele-specific expression in the human heart and its application to postoperative atrial fibrillation and myocardial ischemia.” Martin I Sigurdsson, Louis Saddic, Mahyar Heydarpour, Tzuu-Wang Chang, Prem Shekar, Sary Aranki, Gregory S Couper, Stanton K Shernan, Jon G Seidman, Simon C Body, Jochen D Muehlschlegel (2016). “Genome Medicine” 8(1),127. PMID: 27923400

(05) "Integrated microRNA and mRNA responses to acute human left ventricular ischemia." Saddic LA, Chang TW, Sigurdsson MI, Heydarpour M, Raby BA, Shernan SK, Aranki SF, Body SC, Muehlschlegel JD.

(2015). "Physiol Genomics." 47(10): 455-462. PMID: 26175501

(06) "Gene Expression and Expression Quantifying Trait Loci Analysis of Left Atrium of Patients With Post- operative Atrial Fibrillation." Sigurdsson, Martin I, Mahyar Heydarpour, Louis Saddic, Tzuu-Wang Chang, Stanton K Shernan, Prem Shekar, Sary Aranki, Gregory Couper, Jochen D Muehlschlegel and Simon C Body (2015). "Circulation" 132(Suppl 3): A12905.

(07) "Identification of novel candidate risk genes for atrial fibrillation after cardiac surgery using high- throughput RNA sequencing of human left atrium tissue:o15–07." Sigurdsson, Martin Ingi, Mahyar Heydarpour, Louis Saddic, Tzuu-Wang Chang, Stanton K Shernan, Jochen Danny Muehlschlegel and Simon C Body (2015). “ACTA ANAESTHESIOLOGICA SCANDINAVICA 59,43” WILEY-BLACKWELL 111 RIVER ST, HOBOKEN 07030-5774, NJ USA.

(08) "Selection of RNA Aptamers Against Botulinum Neurotoxin Type A Light Chain Through a Non- Radioactive Approach." Chang Tzuu-Wang, Pavithra Janardhanan, Charlene M Mello, Bal Ram Singh and Shuowei Cai (2016). "Applied biochemistry and biotechnology" 180(1):10-25. PMID: 27085355

(09) "Evolutionary Traits of Toxins." Kumar, Raj, Tzuu-Wang Chang and Bal Ram Singh (2015). "Biological Toxins and Bioterrorism": 527-557.

(10) "The botulinum neurotoxin complex and the role of ancillary proteins."Singh, Bal Ram, Tzuu-Wang Chang, Roshan Kukreja and Shuowei Cai (2014). Molecular Aspects of Botulinum Neurotoxin, Springer New York: 69-101.

(11) In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxin. Chang TW, Blank M, Janardhanan P, Singh BR, Mello C, Blind M, Cai S. “Biochem Biophys Res Commun”. 2010 Jun 11;396(4):854-860. Epub 2010 May 7. PMID: 20452328

(12) Immunological characterization of the subunits of type A botulinum neurotoxin and different components of its associated proteins. Kukreja R, Chang TW, Cai S, Lindo P, Riding S, Zhou Y, Ravichandran E, Singh BR. “Toxicon”. 2009 May;53(6):616-624. PMID: 19673075

(13) Expression, purification and comparative characterisation of enzymatically deactivated recombinant botulinum neurotoxin type A. Weiping Yang, Paul Lindo, Stephen Riding, Tzuu-Wang Chang, Shuowei Cai, Thuan Van, Roshan Kukreja, Yu Zhou, Kruti Vasa, Bal Ram Singh. “The Botulinum Journal (TBJ)”. 2008 Volume 1 - Issue #2:219-241. DOI: 10.1504/TBJ.2008.026477

(14) Horizontal gene transfer may involve Clostridium botulinum neurotoxin evolution. TW Chang, BR Singh. FASEB Journal 2007 Apr;21(5), A622-A622

(15) Botulinum neurotoxin light chain refolds at endosomal pH for its translocation. Cai S, Kukreja R, Shoesmith S, Chang TW, Singh BR. “Protein J.”. 2006 Dec;25(7-8):455-462. PMID: 17115255

(16) "Nucleotide and protein sequence analyses reveal role of neurotoxin associated proteins in food poisoning potential of Clostridium botulinum." Chang TW and BR Singh (2004) May:18(8), C244-C244. FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL 9650 ROCKVILLE PIKE, BETHESDA, MD 20814- 3998 USA.

(17) Analysis of the Drosophila Gene for the Laminin B1 Chain. Ch Gow, Huiyun Chang, Cj Lih, TW Chang, Chofat Hui. “DNA and Cell Biology”. 1993 Sep;12(7), 573-587. PMID: 8397815

(18) “PEER-residuals based cis-eQTL & GSEA Analyses of Human Left Ventricle.” Chang TW, Saddic L, Sigurdsson MI, Muehlschlege JD. (2018)

(19) "Detection of eQTLs in Human Left Atrium and Their Association with Atrial Fibrillation Using Hidden Expression Factor Analysis (HEFT)." Sigurdsson, Martin I, MD Louis Saddic, Tzuu-Wang Chang, Stanton K Shernan and Jochen D Muehlschlegel. (2017)

(20) “The Long Noncoding RNA Landscape of Ischemia in the Human Left Ventricle is Coordinated With Coding Gene Expression.” Louis Saddic, Tzuu-Wang Chang, MI Sigurdsson, M Heydarpour, Benjamin Raby, Stanton K Shernan, Jon Seidman, Sary Aranki, Simon Body, Jochen D Muehlschlegel. (2017)

(21) “Allele-specific Expression in the Human Heart and its Application to Study Disease States.” MI Sigurdsson, LA Saddic, M Heydarpour, TW Chang, SK Shernan, Seidman G Jon, Simon Body, Jochen D Muehlschlegel. (2017)

Citation Records: http://scholar.google.com/citations?user=ji_6gvUAAAAJ&hl=en P H .D D ISSERTATION TITLE

SEQUENCE ANALYSES & NOVEL ANTIDOTES DEVELOPMENT OF BOTULINUM NEUROTOXIN (2011)

( https://drive.google.com/open?id=1x2Lkni3IWKUq4TBjvMX49P63P_O-8aZ4 ) C ODING E XAMPLES

Transcribe_CABG: Please visit my personal GitHub ( https://github.com/alextwc/Transcribe_CABG ) to view the codes that I composed for cis-eQTL, GWAS and other Bioinformatics analyses (using R, Python, UNIX Shell scripts including BASH, AWK+SED, and Java Script) C & Mathematica Coding: These two PDF files ( https://github.com/alextwc/Homework_Assignments ) contain the heuristic algorithms that I developed to solve the analytical issues of our bench work data during my PhD student stage.

P ERSONAL L INKS

My Linkedin: https://www.linkedin.com/in/tzuuwangchang/ My Google Scholar: https://scholar.google.com/citations?user=ji_6gvUAAAAJ&hl=en https://books.google.com/books/about/Sequence_Analyses_and_Novel_Antidotes_De.html?id=vRXvZwEACAAJ&hl=en R EFERENCE C ONTACTS

(1). Weiliang Qiu (PhD) (My co-advisor at CDNM/BWH/HMS) [ ac80ki@r.postjobfree.com ] [ TEL:508-***-**** ]

* Assistant Professor in Statistics, Public Health School of Harvard Medical School

* Associate Director in Biostatistics of Sanofi Pharmaceuticals

(2). Paul L. Huang (MD,PhD) (My first Post-Doc advisor) [ ac80ki@r.postjobfree.com ] [ TEL:617-***-**** ]

* Director, Cardiac Metabolic Syndrome Program [ ac80ki@r.postjobfree.com ]

* Professor of Medicine, Harvard Medical School (HMS)

* Physician in Cardiology, Massachusetts General Hospital (MGH)

* Associate Director, Cardiovascular Research Center, MGH

* Fellow of American College of Cardiology

* Advisory Committee Member of "Johnson Space Center, NASA"

* More than 200 publications: Nature(4), Science(2), Cell(5), PNAS(14), NEJM(1), JAMA(1), Stroke(9), Neuroscience(8), Gene(3), Circulation(10)

(3). Ken Levasseur (PhD) (My mathematics professor) [ ac80ki@r.postjobfree.com ] [ TEL:978-***-**** ]

* Professor of Mathematical Science, UMASS Lowell

* More than 90 important publications in outstanding research journals

* http://www.uml.edu/Sciences/mathematics/faculty/levasseur-kenneth.aspx

(4). Alex Fowler (PhD) (The Director of of my PhD Program) [ ac80ki@r.postjobfree.com ] [ TEL:508-***-**** ]

* Associate Provost for Graduate Studies, UMASS Dartmouth

* He was the teacher who taught me two courses and examined my PhD Qualify Exam "Thermodynamics"

* He was the Director of Biomedical Engineering Graduate Program

* More than 80 important publications in outstanding research journals

(5). Chi-Hau Chen (PhD) (My PhD program committee member) [ ac80ki@r.postjobfree.com ] [ TEL:508-***-**** ]

* IEEE fellow

* He is the Chancellor Professor of Electrical & Computer Engineering department, UMASS Dartmouth. M Y S IGNATURE

Alex Tzuu-Wang Chang, Ph.D.

ac80ki@r.postjobfree.com

My USA cellphone: 508-***-****

PhD in Biomedical Engineering

M.S. in Computer & Information Science

M.S. in Molecular Genetics

M.S. in Biotechnology

Microsoft Certified System Engineer

Now we realize that the phenomena of chemical interaction and, ultimately, of life itself are to be understood in terms of electromagnetism.

[Feynman Lectures on Physics Volume II, Section 1-6, Page 1-10]

The link of my PhD dissertation:

https://drive.google.com/open?id=1x2Lkni3IWKUq4TBjvMX49P63P_O-8aZ4

Contact this candidate