Plant Project

Umashankar Upadhyay

[Sec. ** Padam Nagar, Greater Faridabad, Haryana]

[+91-988*******] • [*************@*****.***]

Overview

Looking forward the challenges in microbiology and molecular biology that require application of my functional experience and skill to working environment, to actively participate to solve inherently related issues in modern organizations and prove myself in the field of Microbiology. Interested in working with Plant-Microbes Molecular interactions, Infectious Disease, Instrumentation and molecular biology techniques in order to widen my area of knowledge.

Experience

2. [Sept. 2017- June 208] [Paid Volunteer]

Shoolini University, Solan

Building Relations and projects from concept of compeilation and designing meeting strategies

Manage all international students in the organization

Organized Various International Relationship Events Education Qualification

2016- 2018 Master of Science (Biotechnology)

Shoolini University, Himachal Pradesh (India)

Relevant subjects: Immunology, Biochemistry, Cell biology, Animal biotechnology, Plant biotechnology, Environmental biology, Enzymology, Developmental biology, Bioprocess technology, Food Biotechnology, Rcombinant DNA Technology, Molecular Biology, Endochrinolgy,Biostatistics etc.

Score: 76.4%

2013- 2016 Bachelor of Science (Biotechnology)

GLA University, Mathura (UP)

Relevant subjects: Immunology, Biochemistry, Cell biology, Animal biotechnology, Plant biotechnology, Environmental biotechnology, Enzymology, Rcombinant DNA Technology, Molecular Biology, Developmental biology, Bioprocess technology, Food Technology Biostatistics, Plant Science, Human Physiology etc. Score: 77.5%

2011 -2013 Higher Secondary (11th

-12th

)

Central Board of Secondary Education

Relevant subjects: Biology, Physics, Chemistry, Physical Education, English. Score: 56%

1. [10 Sept. 2018- Present] [Asst. professor]

Greater Noida Group of Institutions, Greater Noida

Taking Lectures of Cell Biology and Genetics

Taking Motivational and Personality Development Sessions Skills:-

Wet Lab Skills:-

1. Exposure of working in BSL3 lab

2. General laboratory: Sterilization, Media preparation, Titration, Filtration, Sonication. 3. Tools: Microscope (simple, compound, inverted), PCR, Real Time PCR, LAF, Autoclave, Micropipettes, Centrifuge, Spectrophotometer, Incubators (CO2), Haemocytometer, ovens. 4. Techniques: Molecular diagnostic technique, Chromatography, Enzymatic assay, DNA and RNA extraction, Cell Viability Test, Gel Electrophoresis, Immuno-diffusion, Distillation, Aniti-Microbial assay, Immunoassays, Haemocytometer, and ELISA, Blood grouping, Widal, Quantification and Qualification assay.

Dry Lab Skills:-

1. SPSS, Operating systems (windows, Ubuntu,)

2. Bioinformatics tools: BLAST, FASTA, MEGA 7, Chromas, Clustal Omega etc. Soft Skills:-

Good Listener, Team Management, Leadership, Motivational Speaker etc. Project Highlights:-

Project 1: -

Complete a project on clinical utility of TaqMan probe in quantification of hepatitis B virus by Real Time PCR.

DESCRIPTION

Hepatitis B is an infectious disease caused by the hepatitis B virus (HBV) which affects the liver. It can cause both acute and chronic infections. Many people have no symptoms during the initial infection. Some develop a rapid onset of sickness with vomiting, yellowish skin, feeling tired, dark urine and abdominal pain. HBV has eight genotypes (A-H) due to variation in nucleotide sequences greater than 8%. Various publications are found that the clinical course and outcome of antiviral therapy dependent on the genotype of the infecting HBV strains. After that real time come in field of molecular diagnostic of epidemic diseases. The Roche Real Time PCR accurately identifies the genotype in one step reaction by means of primer specificity. It provides a useful tool for rapid detection and would make large scale longitudinal HBV related studies simple & feasible. TaqMan hepatitis B virus (HBV) analyse specific reagent is designed for the quantification of HBV DNA in serum or plasma. Analytical sensitivity and precision were assessed with commercially available HBV standards, while clinical serum specimens from HBsAg seropositive patient and healthy blood donor were used to determine clinical sensitivity, specificity and correlation with other commercially available assays

Project 2: -

Characterization of Gut Microflora of Humans (Homo sapiens) Living in the Tribal Areas of Himachal Pradesh.

DISCRIPTION

The relevance of the gut microbiota to human health is increasingly appreciated. The objective of this study was characterization of the gut microbiota of a group of teenage and children living in tribal areas of Kinnaur district of Himachal pradesh, India. Faeces were collected from 10 healthy tribal teenagers and child of 1- 25 years from the Karchham village of Kinnaur district, Himachal pradesh. DNA was extracted, and amplified by using 16s rRNA universal bacterial primers and they showed approx. 1500bp segments comprising region of 16S rRNA gene were amplified, barcoded and sequenced. Totally 600 and 750 base pairs good-quality reads were analyzed. Tribal peoples consumed a millets-based and rice-based diet, ate cereals and traditional Himachali fermented food every day, and consume milk or milk products. Meat once a week. In both groups, Escherichia was the most abundant phylum, followed by various uncultured bacterial clones. Phylogenetic analysis of major microbiota indicated clustering of microbial groups at different major branch points for tribes. Genus Escherichia and various uncultured bacterium clones constituted the bulk of the faecal microbiota, while significant differences in composition between the groups were probably due to differences in diet and lifestyle and age factor. Publications: -

1) IS6110 Gene in Mycobacterium Tuberculosis Complex in Tuberculosis Meningitis- Clinical Application for Disease Monitoring (Scholars Academic Journal of Biosciences) Tuberculosis meningitis (TBM) is the most common form of chronic infection of central nervous system. Regardless of the extent of the problem, the general diagnostic outlook is discouraging specifically; there is no generally accepted early confirmative diagnostic protocol available for TBM. In the current work, 16 Cerebrospinal Fluid (CSF) cases were considered for the molecular characterization.02 CSF came positive for the Nested PCR targeting IS6110 gene, considered to be positive for the presence of mycobacterium tuberculosis DNA. It was also seen that the positive cases were with increased ADA titer. The clinical manifestations of the positive cases were matching with those for the tuberculosis meningitis. 2) High-Risk Human Papilloma Virus (HR-HPV) Genotyping in Female with Abnormal Cervix

(Scholars Academic Journal of Biosciences)

Human Papilloma Virus (HPV) infection is transmitted primarily via sexual and affects mostly basal epithelial cells of the skin or inner lining of tissues and are categorized as cutaneous types or mucosal types. It is known that HPV infection is strongly related to the development of cervical cancer, as viral DNA is detected in 99% of cervical cancer cases worldwide. Proposed study includes 14 cervical brushing, collected from females with cytologically abnormal cervix. The target genes E6 and E7 were amplified by Conventional PCR. Three cases with High grade squamous intraepithelial lesion (HSIL) came positive. Further the positive cases were digested by restriction endonucleases enzymes for RFLP and the amplicon size for high risk HPV type 16 and type 18 were obtained at 452bp and 455bp respectively. Three cases came positive, out of which HPV type 16 came in two cases with one HPV type 18. High risk HPV characterization at an early stage will prevent further progression of the disease. Technical Trainings: -

1. Training on advanced industrial techniques (Helix Biogenesis Ltd., Noida) 2. Two months training on advanced molecular techniques (SGRRI, Dehradoon) Conferences, Seminar and Workshop: -

1. Attend symposium on “Accelerating Biology, 2015” 2. Attend national seminar on “Detection and Treatment of Cancer using Nano and Conventional Technologies, 2015”

3. Attend National Conference on “Environment and Natural Resource Management, 2017” 4. Attend a Workshop on “Patent Filing and Geographical Indications” 5. Attend “National Seminar on Innovation and Challenges in Basic And Applied Sciences, 2017”

6. Attend a National Seminar on “Health and Nutrition” 2017 7. Organize a workshop on Biotechnology Techniques 8. Organize a workshop on intellectual property rights and geographical indications Achievements: -

1. In Top 20 ranks (all India) of BEST ABLE 9

th

Edition 2017

2. Merit in Research

3. 1st

Prize for the Power point presentation in college

4. 3

rd

Prize in Quiz Competition

Extra-Curricular Activities: -

1. Organizer college fest (SPANDAN-2015&2016)

2. Marketing coordinator for college fest

3. Team Head in College Fest (Moksh 2K17 and 2K18) 4. Stem Cell Donor in DATRI

5. Voluteering in Various Events

6. Certificate of Bhartiya Sanskriti Gyan Pariksha from 2006-2008 7. Certificate of Akhil Bhartiya Sanskriti Gyan Pariksha Interests: -

1. Travel: backpacked across different cities of India 2. Keen to Research

3. To know about new Techniques

4. To make new friends

5. To Help others

Career Objective

To Build a long term career in Research and academics with the opportunities for career growth and enhance my education and professional skills in a stable and dynamic workplace and be able to solve problems in such creative and effective manner in challenging position. REFRENCES

Prof. Saurabh Kulshrestha

Designation: Professor, Department of Biotechnology, Shoolini University E-mail ID: *******************@******************.*** Cell No. : +91-962*******

Research area: Molecular Plant Microbe Interactions, Mycovirology Academic Degrees: Ph.D., CSIR IHBT, Palampur

Postdoc, University of Kentucky USA (2005-2007)

Postdoc, Max Planck Institute for Plant Breeding Research Koln, Germany (2008-2009) Mr. Shubham Upadhyay

Designation: Patent and design Examiner, Ministry of Commerce and Industry, Govt. of India, New Delhi

Cell No. : +91-880*******

Email ID: ************@*****.***

Prof. Anuradha Sourirajan

Designation: Dean, Department of Biotechnology, Shoolini University E.Mail- ********@******************.***

Cell No. - +91-980*******

Research Area: Protein and Gene Expression and Characterization Academic Degrees: Ph.D., IISc, Bangalore

Postdoc. NIH USA

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