Nate Dudley, Ph.D.

INDEPENDENCE, MO ac7xd7@r.postjobfree.com

805-***-****

Education

University of North Carolina, Chapel Hill NC 2000 - 2006

Ph.D. in Biology

Princeton University, Princeton NJ 1995 - 1996

Molecular Biology

Hampshire College, Amherst MA 1989 - 1994

Bachelor of Arts in Molecular Biology

Relevant Skills

Molecular / Microbiology/ Biochemical Techniques: PCR, qPCR, RT-PCR, SDS-PAGE, Western/Northern Blotting, HPLC, molecular cloning, microbiology, immunohistochemistry/precipitation, ECL-based assays, recombinant DNA/Protein design/isolation, antibody design/purification, HeLa Cell line maintenance and recombinant protein purification, aseptic culture techniques, a solid understanding of gene expression microarrays, vaccination, challenge and necropsy/tissue isolation,

Cell Biology / Microscopy: DIC/Epi-fluorescence, live-imaging analysis, spinning disc/LSM Confocal microscopy, image processing/deconvolution, DNA/protein sequence database mining/analysis and animal dissections

Reverse / Forward Genetics: Chemical/UV-Mutagenesis, DNA Transfection/Transformation, RNA isolation, siRNA-design, and in vitro/ in vivo RNAi

Supervising / Managerial / Mentoring: Directly supervised undergraduate, graduate students, and post-docs. Human Resources: PeopleSoft/Admin, Assist employees with Visas (H1-B, J-1, and Permanent Residency), Designed and modified experimental protocols, managed budgets, wrote grants and attained funding, prepared manuscripts for publication, and organized group meetings/journal clubs

Research / Work Experience

Research Scientist/Project Manager, University of Kansas Medical Center 1/2017 - 8/2018

Manage the day to day research operations of the basic sciences unit at the Cardiovascular Research Institute

-Oversee/Manage, interpret, and troubleshoot the basic research efforts of all staff

-Develop study protocols, optimize, and resolve methodological problems with research projects

-Review technical documentation and research data; write and prepare data for publication

Technician II - Research and Development, Ceva Biomune Lenexa,KS 10/2016 – 1/2017

Animal trials/studies, necropsy, Salmonella challenge, general microbiology/molecular biology

-R&D for the submission of vaccine trial/data to the FDA

-Perform toxicology studies of virulent strains, including ELISAS and LD-50 assays

-Maintain and ensure all documentation is in compliance with Federal Regulations

Executive Director - TrySci Community Biolabs, Kansas City, MO 04/2014 – Present

A community biolab open to the public and tasked to educate the citizen through hands on scientific research

-Visit us at www.trysci.org

Post-Doctoral Fellow - University of California, Santa Barbara CA 05/2006 - 08/2012

President’s Postdoctoral Fellow: Department of Molecular, Cellular, and Developmental Biology

As a U.C. President’s Postdoctoral Fellow, I developed an independent research program using molecular, genetic, and biochemical approaches to study the regulatory networks at play in balancing pluripotency and differentiation. My research focused on understanding the mechanisms that maintain the developmental potential of the germline. Using C. elegans, I identified a number of genes that disrupt germline potential, resulting in germ cells inappropriately initiating a complex program of somatic differentiation.

PhD Candidate - University of North Carolina, Chapel Hill NC 06/2000 - 05/2006

Lineberger Cancer Center Graduate Fellow: Department of Biology.

As a graduate student I was trained as a cell and molecular biologist where I studied the molecular mechanisms of RNAi. Early in the RNAi field, I successfully developed a unique and efficient RNAi-based screening strategy to identify many novel RNAi components.

University of Pennsylvania, Philadelphia PA 03/1998 - 06/2000

Research Specialist III: UPENN School of Medicine, Department of Genetics

At UPENN, I investigated Ras signal transduction using molecular, biochemical, and genetic techniques

Princeton University, Princeton NJ 06/1997 - 3/1998

Research Specialist I: Department of Molecular Biology, Lewis Thomas Laboratory

At Princeton University I performed site-directed mutagenesis on adenovirus IVa2 promoter and performed biochemical purification of mutant p53 from HeLa cells

University of California, San Francisco, CA 09/1994 - 07/1995

Research Associate: UCSF School of Medicine, Department of Biochemistry and Biophysics

At UCSF, I performed enzyme kinetic assays on candidate inhibitors of thymidylate synthase. I designed mutant forms of the protein, purified the protein from bacteria over columns, used HPLC and ran kinetic assays

Institute of Toxicology, Würzburg, Germany 07/1993 - 01/1994

Drohan Visiting Research Associate: Institute of Toxicology, University of Würzburg

As a Drohan Scholar, I studied the loss of heterozygosity in malignant melanomas of tissue samples from cancer patients using RT-PCR and standard molecular techniques

Marine Biological Laboratory, Woods Hole, MA 05/1992 - 09/1992

W.T. Porter Research Fellow

As a Porter Fellow, I performed tissue dissections of the giant axon of the squid and used advanced live-video microscopy to analyze motor proteins associated with fast axonal transport along microtubules

McKesson Foundation Fellow, Sri Lanka 01/1992 - 02/1992

Field Assistant - Funded by the McKesson Foundation and the NIH

As a field assistant I traveled to the island nation of Sri Lanka and collected data on high-level radon emissions found throughout the island

Howard Hughes Fellow, Hampshire College, Amherst, MA 09/1989 - 05/1994

HHMI Undergraduate Fellow

As a Howard Hughes Undergraduate Fellow, I worked in Molecular and Neurobiology labs studying gene expression using genetic and biochemical techniques

Teaching Experience

Undergraduate Research Advisor: MCDB 099, U.C. Santa Barbara 2006 - 2012

Directed and mentored 5 undergraduate researchers for the last six years

Research Mentorship Program, Summer Sessions, U.C. Santa Barbara 2008 - 2011

Mentored and directed 8 local minority high school students in Summer research over 3years

Course Instructor MCDB:112L (Winter Quarter), U.C. Santa Barbara 2009

Instructor for Developmental Biology lab for Senior Bio majors

Undergraduate Research Advisor, UNC, Chapel Hill 2002 - 2005

Oversaw and trained three bright undergrads in MCDB research

Teaching Assistant UNC, Chapel Hill 2001

Biology 50 (Genetics and Molecular Biology)

Instructor Microbiology laboratory, Hampshire College, Amherst, MA 1992 - 1993

Program aim: promote women and minority interest in science among 6th and 7th graders

Instructor / Resident Assistant – Summer of Science 1991

Hampshire College, Amherst, MA - program aim: promote women and minority

interest in science among 6th and 7th graders

Course Instructor - Gene Cloning 1991

Hampshire College, Amherst, MA

Winter term Laboratory Course

Honors and Awards

2008-11 National Institutes of Health, NICHD Postdoctoral Diversity Fellow

2006-08 UCOP Presidential Postdoctoral Fellowship – University of California Office of the President

2005 Lineberger Cancer Center Graduate Fellow Award – UNC Chapel Hill, NC

2004 BGSA Outstanding Graduate Student Lecturer Award

2003 Awarded best talk, AGEP EMERGE Conference, Atlanta, GA

2000-02 NSF Graduate Fellowship, University of North Carolina

(Administered by Dept. of Ed., U. North Carolina, Chapel Hill)

1989-94 Howard Hughes Medical Institute Fellow, Hampshire College

1993 Drohan Scholarship for Senior Thesis work in Germany

1992 W.T. Porter Fellowship, Woods Hole Marine Biological Laboratory

1991 Recipient of NSF Award for travel to Sri Lanka (Administered by Hampshire College)

Published coverage of PhD work:

Matzke M (2002) RNA Interference. The Scientist 16:41.

(A New & Notable abstract about Dudley et al. 2002). A “Must Read” paper at Faculty of 1000 (Dudley et al. 2002) (http://www.f1000biology.com/article/id/1005173/evaluation)

Weitzman JB (2002) RNAi to RNAi. Genome Biology, March 2002, and reprinted in The Scientist,

March 26, 2002. (A Research News article about Dudley et al. 2002).

“UNC’s Bob Goldstein Talks About Getting into RNAi and Hunting for Genes”

(An interview published in RNAi News on Dudley et al, Feb 13, 2004).

Press release about Dudley et al 2002: ScienceDaily April 5, 2002

(http://www.sciencedaily.com/releases/2002/04/020*********.htm)

Publications

Adamiak M, Cheng G, Bobis-Wozowicz S, Zhao L, Kedracka-Krok S, Samanta A,Karnas E, Xuan YT, Skupien-Rabian B, Chen X, Jankowska U, Girgis M, Sekula M,Davani A, Lasota S, Vincent RJ, Sarna M, Newell KL, Wang OL, Dudley N, Madeja Z,

Dawn B, Zuba-Surma EK. (2018) Induced Pluripotent Stem Cell (iPSC)-Derived ExtracellularVesicles Are Safer and More Effective for Cardiac Repair Than iPSCs. Circ Res. 122(2):296-309.

Dudley, N.R. and Rothman, J.H. (in prep 2018). Calcium signaling regulates the totipotent state of the C. elegans germline.

Djabrayan, JVN, Dudley, N.R., et al. (2012) Essential role for Notch signaling in restricting developmental plasticity.

Genes and Development 2012 Nov 1;26(21):2386-91

Dudley, N.R., A.Z. Amin and B. Goldstein (2005). Genes Required for RNA Interference, chapter in "RNA Interference: From Basic Science to Drug Development", Cambridge University Press, Cambridge, UK, edited by K. Appasani.

Dudley, N.R. and B. Goldstein (2005). RNA Interference in Caenorhabditis elegans, chapter in "RNA Silencing: Methods and Protocols", Methods in Molecular Biology, vol 309, Humana Press, edited by G. Carmichael. (An invited review).

Dudley, N.R. and B. Goldstein (2003). RNA interference: Silencing in the cytoplasm and nucleus. Curr Opin Mol Ther 5:113-117.

Dudley N.R., Labbe J-C, and Goldstein B. (2002). Using RNA interference to identify genes required for RNA interference. Proc. Natl. Acad. Sci. USA 99: 4191-4196

Sundaram MV and Dudley NR (2000)

Possible perils pertaining to the lin-31 promoter and Pn.p cells. Worm Breeder's Gazette 16 3:21

Papp T, Jafari M, Diener U, Dudley N.R., Schiffmann D. (1993). Modified ASRA facilitates the characterization of activating point mutations in tumors, in which cancer cells constitute only a minor part of the investigated tissue.

PCR Methods Appl. Oct;3(2):125-6

Langford, G.M., Dudley N.R. et al.. (1992). “Axoplasmic organelles move on exogenous actin filaments”, Biological Bulletin.

Talks at International/National Meetings and Invited Lectures

Dudley, N.R. and Rothman J.H. (2009)

Platform Talk, Postembryonic Cell fate: RNAi Screen for genes required to maintain totipotency in germline

2009 International C. elegans meeting, Los Angeles, CA

Dudley, N.R. and Rothman J.H. (2008)

Platform Talk, Understanding the molecular mechanisms of totipotency in the C. elegans germline

Santa Cruz Developmental Biology Meeting (SCDB)

Dudley, N.R. and Rothman J.H. (2008)

Departmental Seminar, MCDB

Investigating the molecular mechanisms of totipotency in the C. elegans germline

UC Santa Cruz, April 2008

Dudley, N.R. and Rothman J.H. (2007)

Platform Talk, Postembryonic Cell fate: RNAi Screen for genes required to maintain totipotency in germline

2007 International C. elegans meeting, Los Angeles, CA

Dudley, N.R. and Goldstein B (2005)

Platform Talk, RNAi and Gene Silencing: The Identification of six new genes required for RNAi

2005 International C. elegans meeting, Los Angeles, CA,

Talks at International/National Meetings and Invited Lectures Cont’d

Dudley, N.R. and Goldstein B (2004)

Invited Speaker, A role for nuclear proteins in RNAi in animal cells

2004 American Association for Cancer Research Annual Meeting, Orlando, FL

Dudley, N.R. and Goldstein B (2003)

Plenary Talk, Unwinding the mysteries of RNA interference: A conserved RNA helicase with

double-stranded RNA binding domains is required for RNAi in C. elegans

2003 International C. elegans Meeting, Los Angeles, CA

Dudley, N.R., Labbe, J-C., and Goldstein B (2001)

Platform Talk, RNAi and Gene Silencing: Using RNAi to identify new components of the RNAi machinery

2001 International C. elegans Meeting, Los Angeles, CA

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