Mian Cai, Ph.D.

**** ********* **, ********, ** *0777

Phone: 410-***-**** Email: ac7jos@r.postjobfree.com

Citizenship : United States of America

KEY COMPETENCIES

R&D Senior Scientist with experience and expertise in molecular biology and microbiology.

Extensive hands-on experience working in a BSL-2 laboratory with responsibilities designing, performing PCR, qPCR, ELISA, high-resolution melt analysis (HRM), DNA/RNA/protein extraction, western blot.

Gene cloning, expression, knock-out, and complementation analysis.

Protein expression, purification, and characterization.

Experience working in a matrixed, cross-functional team environment developing innovative products under design control process.

Experience in clinical microbiology lab.

Fundamental techniques for bacterial detection, isolation, purification, identification, and characterization.

Well-versed in Microsoft Office/Excel/power point presentation; Statistical analysis software using GraphPad Prism; Primer and probe design software using Alamut Visual, Visual OMP, ThermoBLAST, uMelt, and PrimerQuest Tool).

Strong critical thinking and problem-solving skills.

Fluent in both English and Chinese.

EDUCATION

Washington State University

Pullman, WA

Doctorate’s Degree, microbiology

Shandong University

Jinan, Shandong, China

Master's Degree in microbiology

Shandong University

Jinan, Shandong, China

Bachelor’s Degree in microbiology

EMPLOYMENT HISTORY

Senior Scientist, Microbiology and Assay Development

Canon US Life Sciences, Inc. 2007 –Present

Rockville, MD

Development of quantitative PCR assays for infectious diseases.

Development of PCR-based assays with high-resolution melting (HRM) analysis for human genotyping on commercially available and novel in-house instruments.

Manage and mentor junior scientists and interns.

Research Associate

Johns Hopkins University School of Medicine 2003 – 2006

Department of Pathology, Division of Medical Microbiology

Baltimore, MD

Development and validation of molecular diagnostic tests for detection/identification of bacterial pathogens and their toxins, writing standard protocols, and training medical technicians.

Molecular epidemiological studies of methicillin resistant Staphylococcus aureus (MRSA) and Clostridium difficile.

Clinical trials with pharmaceutical companies.

Postdoctoral Fellow

Johns Hopkins University School of Medicine 2002 – 2003

Department of Pediatrics, Division of Infectious Diseases

Baltimore, MD

Characterization of molecular pathogenesis of E. coli K1 strain that causes neonatal meningitis.

Characterization of the functions of ibeB, fimH, ompA genes of strain E. coli E44 in its penetration of blood brain barrier of humans.

Research Assistant

Washington State University, School of Molecular Biosciences 1997 – 2001

Pullman, WA

Genetic and Biochemical Studies of Pentachlorophenol Degradation by Sphingobium chlorophenolicum ATCC 39723.

Assistant Professor and Director of the Microbiology Lab

Shandong University 1986 –1993

Jinan, Shandong, China

Teaching classes:

General microbiology and microbiology laboratory.

Principles of gene manipulation.

PUBLICATIONS

1.J.E. Farley, P.D. Stamper, M. Cai, T. Howard, S. Speser, and K.C. Carroll, “Comparison of the BD GeneOhm™ MRSA PCR Assay to culture using BBLTM CHROMagar™ MRSA for detection of MRSA from nasal surveillance cultures in an at-risk community population,” J Clin Microbiol. 2008 Feb; 46(2):743-6.

2.C.P. Johnston, A.B. Stokes, T. Ross, M. Cai, K.C. Carroll, S.E. Cosgrove, and T.M. Perl, “Staphylococcus aureus colonization in healthcare workers exposed to a patient population with a high prevalence of methicillin-resistant staphylococcus aureus (MRSA),” Infect Control Hosp Epidemiol. 2007 Dec; 28(12):1404-7.

3.M.E. Reller, C.A. Lema, T.M. Perl, M. Cai, T.L. Ross, K.A. Speck, and K.C. Carroll, “Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for the detection of toxigenic clostridium difficile,” J Clin Microbiol. 2007 Nov; 45(11):3601-5.

4.P.D. Stamper, M. Cai, C. Lema, K. Eskey, and K.C. Carroll, “Comparison of the GeneOhm VanR Assay to culture for the identification of vancomycin-resistant enterococci in rectal and stool specimens,” J Clin Microbiol. 2007 Oct; 45(10):3360-5.

5.P.D. Stamper, M. Cai, T. Howard, S. Speser, and K.C. Carroll, “Clinical validation of the molecular-based BD GeneOhmTM StaphSR for the direct detection of staphylococcus aureus and methicillin-resistant staphylococcus aureus in positive blood cultures,” J Clin Microbiol. 2007 Jul; 45(7):2191-6.

6.C.H. Teng, Y. Xie, S. Shin, F. Di Cello, M. Paul-Satyaseela, M. Cai, and K.S. Kim, “Effects of ompA deletion on expression of type 1 fimbriae in Escherichia coli K1 strain RS218 and on the association of E. coli with human brain microvascular endothelial cells,” Infect Immun. 2006 Oct; 74(10):5609-5616.

7.C.H. Teng, M. Cai, S. Shin, Y. Xie, K.J. Kim, N.A. Khan, F. Di Cello, and K.S. Kim, “Escherichia coli K1 RS218 interacts with human brain microvascular endothelial cells via type 1 fimbria bacteria in the fimbriated state,” Infect Immun. 2005 May; 73(5):2923-31.

8.S. Shin, G. Lu, M. Cai, and K.S. Kim, “Escherichia coli outer membrane protein A adheres to human brain microvascular endothelial cells,” Biochem Biophys Res Commun. 2005 May 20; 330(4):1199-204.

9.J.S. Francis, M.C. Doherty, U. Lopatin, C. P. Johnston, G. Sinha, T. Ross, M. Cai, N.N. Hansel, T.M. Perl T, J.R. Ticehurst, K.C. Carroll, D.L. Thomas, E. Nuermberger, and J.G. Bartlett, “Severe community-onset pneumonia in healthy adults caused by methicillin-resistant staphylococcus aureus carrying the panton-valentine leukocidin genes,” Clin Infect Dis. 2005 Jan 1; 40(1):100-7.

10.T.L. Ross, E.P. Fuss, S.M. Harrington, M. Cai, T.M. Perl, and W.G. Merz, “Methicillin-resistant staphylococcus caprae in a neonatal intensive care unit,” J Clin Microbiol. 2005 Jan; 43(1):363-7.

11.M. Cai, and L. Xun, “Organization and regulation of pentachlorophenol-degrading genes in sphingobium chlorophenolicum ATCC 39723,” J Bacteriol. 2002; 184(17):4672-4680.

12.L. Xun, J. Bohuslavek, M. Cai, “Characterization of 2,6-dichloro-p-hydroquinone 1,2-dioxygnease (PcpA) of sphingomonas chlorophenolica ATCC 39723,” Biochem Biophys Res Commun. 1999 Dec 20; 266(2):322-5.

ABSTRACTS FOR POSTER OR ORAL SESSIONS

1.M. Cai, Z. Kabir, Y. Machida, J. Huuskonen, L. Jiang, and I. Knight, “A robust, PCR-based assay for detecting SMN1 and SMN2 copy number variation using high-resolution melting analysis,” 2017 ACMG Annual Clinical Genetics Meeting, March 21-25, 2017, Phoenix, AZ.

2.M. Cai, L. Jiang, Z. Kabir, S. Paquerault, J. Huuskonen, and I. Knight, “A robust, PCR based assay for detection SMN1 copy number variation and performance using three commercial instruments,” ASHG 2016, October 18-22, 2016, Vancouver, Canada.

3.L. Jiang, L. Papazisi, R. Ndono, F. Mokeyo, M. Cai, J. Huuskonen, and I. Knight. “Novallele HRM-based genotyping assasy for detection of mitochondrial DNA mutations,” ASHG 2016, October 18-22, 2016, Vancouver, Canada.

4.M. Cai, A. Phillippy, E. Taviani, K. Mclver, A. Huq, S. Salzberg, R. Cowwell, and I. Knight. “Detection of Burkholderia pseudomallei and Burkkhollderia mallei using a large-scale, comparative genomics approach,” the 109th ASM General Meeting, May 17 - 21, 2009, Philadelphia, PA.

5.L. Jiang, A. Phillippy, K. McIver, M. Cai, E. Taviani, S. Salzberg, R. Colwell, and I. Knight. “Detection and screening of Francisella tularensis and its subspecies using a large-scale, comparative genomics approach,” the 108th ASM General Meeting, June 1 - 5, 2008, Boston, MA.

6.P. D. Stamper, M. Cai, C. Lema, L. Bryan, and K.C. Carroll, “Clinical evaluation of BBL bile esculin azide agar with 6μg vancomycin and hardy bile esculin azide broth with 8μg of vancomycin compared to BHI agars containing 10μg vancomycin for the detection of Vancomycin-Resistant Enterococci (VRE) from rectal and stool specimen,” the 107th ASM General Meeting May 21 - 26, 2007, Toronto, Canada.

7.P.D. Stamper, K. Eskey, M. Cai, L. Bryan, K. C. Carroll. “Clinical evaluation of the BD GeneOhm™ VanR assay molecular-based assay compared to standard culture methods for the detection of Vancomycin-Resistant Enterococci (VRE) from rectal and stool specimens,” the 107th ASM General Meeting, May 21 - 26, 2007, Toronto, Canada.

8.M. Cai, Bhally, H., and Carroll, K., “mecA gene, SCCmec and PVL gene detection in staphylococcus aureus in a tertiary care hospital in Baltimore,” the 42nd Annual Meeting of the Infectious Diseases Society of America (IDSA), September 30 - October 3, 2004, Boston, MA.

9.M. Cai, Bhally, H., Tekle, T., Dick, J., Flayhart, D., and Carroll, K, “Comparison of a real time PCR method to conventional PCR and agar dilution for detection of methicillin resistant staphylococcus aureus,” American Society for Microbiology 2004 General Meeting, New Orleans, LA.

10.Bhally, H., M. Cai, Tekle, T., Dick, J., Flayhart, D., and Carroll, K, “A Comparison of various phenotypic tests for detection of Methicillin Resistance in Staphylococcus aureus,” American Society for Microbiology 2004 General Meeting, New Orleans, LA.

11.M. Cai, and Xun, L., “Elucidation of the rule of pcpD in pentachlorophenol degradation from sphingomonas chlorophenolica ATCC 39723,” the American Society for Microbiology 1999 General Meeting, Chicago, IL.

12.Contribution to GenBank database of National Center for Biotechnology information (NCBI). M. Cai, Xun, L. The nucleotide sequences of pcpEMAC and pcpBDR fragments were deposited in the GenBank database of National Center for Biotechnology Information (NCBI) under accession no. AF512952 and U12290, respectively in 2002.

REFERENCES

C. William Hooker, Ph.D., Research and Development manager

Canon US Life Sciences, Inc.

Rockville, MD

Phone: 202-***-**** (mobile)

E-mail: ac7jos@r.postjobfree.com

Arvind K. Virmani, Ph.D., Principal Scientist

Canon US Life Sciences, Inc.

Rockville, MD

Phone: 301-***-**** (mobile), 301-***-**** (landline)

Email: ac7jos@r.postjobfree.com

Karen C. Carroll, MD, Professor of Pathology

Director, Division Medical Microbiology

The Johns Hopkins University School of Medicine, Department of Pathology

Baltimore, MD

Phone: 410-***-****

E-mail: ac7jos@r.postjobfree.com

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