Gaithersburg, MD 20879
I have worked at various places during my career including military installations, government and state run facilities, and academic institutions. I have worked on research and development portfolio administration for medical program areas. I have worked in government acquisition for vaccine and therapeutic medical countermeasures and assisted in the creation and submission of study protocols, regulatory and clinical documentation, and study results to the FDA on the path to licensure. I have worked in various laboratories on infectious disease, traumatic brain injury, hemorrhagic shock, dietary supplements, and standard reference materials. I have also worked with various software products including Word, Power Point, Excel, Endnote, Adobe Photoshop, Adobe Acrobat, and Adobe Illustrator. I have frequently used Sigma Stat, SPSS, Mathcad, and Graph pad statistical and graphing software to analyze data sets as a routine job function. I have knowledge of Fortran and C++ computer programming languages, SharePoint website administration, and web page design experience.
SUMMARY OF PROFESSIONAL HISTORY
Program Analyst May 2017-Present
DHA, Falls Church, VA
Bioengineer III May 2016-May 2017
MCS, Frederick, MD
Chemist April 2014-April 2016
WRNMMC, Bethesda, MD
Biologist October 2010-March 2014
USUHS, Bethesda, MD
Biochemist August 2006-September 2010
NIST, Gaithersburg, MD
Laboratory Technician November 2005-August 2006
USUHS, Bethesda, MD
Research Assistant/Laboratory Technician September 2002-August 2005
George Washington University, Washington, DC
Junior Systems Analyst June 2001-August 2002
US Army Evaluation Center, APG, MD
DETAILS OF PROFESSIONAL HISTORY
May 2017 – May 2017
Falls Church, VA
I developed the Integrated Project Plans for our medical Research and Development program areas of interest that were then executed by the Component Science and Technology Managers and Component Development Project Managers to address capability gaps and meet service, Joint Staff, and Combatant Commands informal and formal Joint Capabilities Integration and Development System requirements. I also developed a management plan to ensured alignment of the portfolio and programs with goals and priorities for each assigned research program area.
I monitored and assessed the financial performance of all programs and associated projects including obligation and expenditure rates for compliance with resource utilization goals. I attended all DHA pre-milestone and milestone reviews including preparing background and summary reports along with assessment criteria as subject matter input and preparation. I managed the timely and accurate completion of reports to congress, briefs, general reports and other tasks assigned to the division
May 2016 – May 2017
I served as the technical representative on project teams, supporting risk planning efforts and providing advice and assistance through the use of interactive communication with various personnel in the government hierarchy in implementing process improvements for advanced development efforts. I served as the technical lead on internal and external Integrated Program Teams, responsible for organizing meetings, preparing agendas, documenting meeting minutes and managing action items prioritizing program requirements and risks, assisting in determining program budgets and contracts to meet schedule timelines and deliverables. I managed all regulatory and clinical documentation on SharePoint. I participated in the Electronic Document Management System working group to transition my functional area from SharePoint to EDMS. I provided scientific/technology advice, technical oversight, and project management support in the areas of science and technology availability, technology maturity, and applicability of proposed candidate Chemical, Biological, Radiological, Nuclear medical countermeasures through advanced development, production, and fielding to the Program Manager and Deputy Program Manager. I collaborated with both internal and external partners, contributing to data analyses in order to create and submit study results to publications or the FDA. I developed drafts and assist the project team to coordinate responsibilities, oversee quality management, and encourage timeliness of completion of protocols, Investigational New Drug applications, other regulatory submissions, and technical reports.
April 2014 – April 2016
Walter Reed National Military Medical Center
I worked on research protocols that studied the stability of anesthetics, the unique structural peaks for different strains of Leishmaniasis from insect cell culture media, and biomarker discovery for ovarian cancer cells from mammalian cell culture media. I processed protein samples with solid phase extraction and SDS-PAGE gels. I performed various methods on the MALDI TOF/TOF, UPLC/MS, HPLC/MS, HPLC/IT TOF, and PDA detectors to determine concentration, composition and purity. I used various platforms including Waters, Shimadzu, AbSciex, Agilent, and Perkin Elmer and their accompanying software Empower 2, LabSolutions 5, Explorer 3.4, Protein Pilot 5, ChemStation and Syngistix. Data processing was done with the Excel software. The work was conducted in a BSL-2 laboratory. I worked with the IRB to develop protocols and clinical trials in agreement with good clinical practice and good laboratory practice. I worked with the clinics in the “Human Use” and Clinical Trials studies to adhere to the approved protocol, document adverse events, and make any necessary amendments. I managed all regulatory and clinical documentation on SharePoint.
October 2010 – March 2014
Uniformed Services University of the Health Sciences
I managed and maintained the laboratory, managed the property inventory, ordered the supplies for the research projects I designed, and assigned all of the experiments for the laboratory staff to minimize cost and maximize productivity in order to meet the various milestones set up by the grant funding agency. I initiated, designed, and executed research protocols involving traumatic brain injury, post-traumatic stress and hemorrhagic shock. I performed surgeries on anesthetized rats that included cannulation to induce a controlled shock by the Labview hemorrhage software and fluid percussion injury. I performed PDH, Complex IV, and Complex I dipstick as well as BCA assays on the blood and tissue samples that were collected after the experiment. I also completed Corticosterone and Serotonin ELISA assays on the serum from these experiments. I processed the results of the various assays on micro-plate readers. I then analyzed and graphed all of the data from the various assays on the SPSS, Graph pad, and Sigma Stat statistical software. The work was conducted in a BSL-2 laboratory.
August 2006 – September 2010
National Institute of Standards and Technology
I worked on the Proteomics Team of the Mass Spectrometry Group. I performed purity determinations on an amino acid mixture containing 13 different amino acids. I used various methods including HPLC/MS, MALDI, and Differential Scanning Calorimeter to determine purity and composition in a GLP environment. I also used CRP, PSA, and IGF-I ELISA assays to determine the concentration and stability of the sample mixture stored at room temperature over time. I determined the purity and composition of dietary supplements making sure to follow all FDA regulations and guidelines on documentation and study parameters. Data processing was done with the Excel software. I wrote the Report of Analysis on the total protein and density measurements of the amino acids in plasma and serum samples from the National Cancer Institute.
November 2005 – August 2006
Uniformed Services University of the Health Sciences
I worked on a research protocol studying the effects of traumatic brain injuries. I performed surgeries on an anesthetized rat that involved inducing injury with a fluid percussion apparatus. The blood and tissue samples collected post injury were processed using various assays including protein, pyruvate, lactate, cytochrome oxidase and glutathione determinations. I processed the results of the various assays on the UV/VIS Spectrophotometer, Blood Gas Analyzer, Microdialysis Analyser, and microplate reader. I performed the processing of these results of various assays with the Sigma Stat statistical software. The work was conducted in a BSL-2 laboratory.
Research Assistant/Laboratory Technician
September 2002 – August 2005
George Washington University
While attending graduate school I worked in the Physical Chemistry Laboratory as a research assistant. I also worked as the laboratory technician for the general chemistry laboratory. As a research assistant I helped to construct a vacuum apparatus and chemical deposition system. I used time of flight mass spectrometry to determine the structure of pure and doped ice films and their vaporization mechanism in cryogenic conditions. As a laboratory technician I prepared all of the solutions for all the undergraduate students in general chemistry.
Junior Systems Analyst
June 2001 – August 2002
US Army Evaluation Center
Aberdeen Proving Ground, MD
I worked on the materials acquisition process of the chemical/biological protection equipment for the U.S. soldiers in the field. I wrote evaluations of the supportability testing, attainment of key milestones, and logical issues during the acquisition process. I also created summary charts for debriefing to the Army Test and Evaluation Command and the Deputy Chief of Staff for Logistics.
Bachelor of Science in Chemistry- 2001
Hampton University, Hampton, VA
Master of Chemistry- 2005,
George Washington University, Washington, DC
Short Course on Interpretation of Mass Spectra
Developing Robust LC Methods
Chromatographic Selectivity, Resolution, and Sensitivity: The Fundamentals of Method Development
Fundamentals of Solid Phase Extraction
QMicro: Rapid, Simple and Accurate Sizing and Counting of Cells
Fundamentals of Capillary Gel Electrophoresis
To Nanodrop or Not to Nanodrop
DAU Science and Technology Manager Certification Level 1
DAU Program Management Certification Level 1
DAU Business Financial Management Certification Level 1
DAU Information Technology Certification Level 1
GROUPS/ACTIVITIES, HONORS, OFFICIES HELD
American Chemical Society
Certificate of Recognition for Outstanding Performance in Chemistry
Beta Kappa Chi National Scientific Honor Society
V. Sadtchenko, M. Brindza, M. Chonde, B. Palmore, and R. Eom, Journal of Chemical Physics, "The vaporization rate of ice at temperatures near its melting point," 121, 23, 119**-***** (2004).
Pushpa Sharma, Brandi Benford, Zhao Z Li, Geoffrey SF Ling, Journal of Emergencies, Trauma, and Shock, "Role of pyruvate dehydrogenase complex in traumatic brain injury and Measurement of pyruvate dehydrogenase enzyme by dipstick test," 2, 2, 67-72 (2009).
Sharma P, Benford B, Karaian JE, Keneally R, Journal of Emergencies, Trauma, and Shock, “Effects of volume and composition of the resuscitative fluids in the treatment of hemorrhagic shock,” 5, 4, 309-15 (2012).
Xing G, Barry ES, Benford B, Grunberg NE, Li H, Watson WD, Sharma P, Frontiers in Neurology, “Impact of repeated stress on traumatic brain injury-induced mitochondrial electron transport chain expression and behavior responses in rats,” 4, 196 (2013).
Phinney Karen, Guillaume Ballihaut, Mary Bedner, Brandi S. Benford, Johanna E. Camara, Steven J. Christopher, Analytical Chemistry, “Development of a Standard Reference Material for Metabolomics Research,” 85, 24, 11732–11738 (2013).