SOUMINI SENGUPTA

** ***** **** ******* ** *****

C: 412-***-**** Email: ac7hm6@r.postjobfree.com

PROFESSIONAL OBJECTIVE

Biologist seeking to improve the scientific community by performing accurate research and complying with all laboratory safety protocols and standards.

WORK HISTORY

KELLY SERVICES (CLIENT: DOW CHEMICAL)

Research and Developmental Microbiologist Collegeville, PA June 2017 – Till Date

Involved in challenge test for customer samples following GLP standards of BSL-2 labs

Define microbiological risk assessments for laboratory protocols

Responsible for innovative experiments

Works in anaerobic chamber to provide answers to customer needs

Maintain bacterial cultures for challenge tests

Work with biocides to run quick kill and long-term preservative tests

Use of programs like JMP for data analysis and report writing

VILLANOVA UNIVERSITY (BIOLOGY DEPARTMENT)

Teaching Assistant Villanova, PA May 2015 - June 2016

Served as a Teaching Assistant for different courses such as: How Microbes Rule the World, Biology & Politics, Cell Biology, Molecular Biology, Caution! How Drugs Work & their Therapeutic Effect

Responsible for pre-lab lecture by engaging in effective coordination and orchestration of a lab of 15-20 students, grading students’ techniques & lab notebooks

Responsible for maintaining HeLa cells for teaching labs

Experienced in handling mice cells post dissection

Acted as the mediator between students and the Biology department

Proctored exams

VILLANOVA UNIVERSITY (OFFICE OF RESEARCH)

Computer Aid Villanova, PA March 2015 - May 2015

Data processing and analysis

Engaged in cooperative management strategies

BIHTS INSTITUTE OF MANAGEMENT

Lecturer of Microbiology Kolkata, INDIA April 2010 - October 2012

Taught undergraduates’ entry level Biology: Virology, Microbiology, Food Science and how it relates to food industry

Member of school administration involved in looking after welfare of students

Programmed computers to store, process and analyze student data

INSTITUTE OF HOTEL AND RESTAURANT MANAGEMENT

Faculty of Microbiology Kolkata, INDIA July 2008 - March 2010

Responsible for teaching Microbiology highlighting food industry

Conducted laboratory experiments engaging students

LABORATORY SKILLS

Use of Flow Cytometer for cell detection

Anaerobic Chamber

Biocide Handling

Cell-based Immunology Assay

Cell Culture

Media Preparation

ELISA assay

Microbiology Techniques

Autoclave Usage

Serial Diluter Usage

Biosafety Hood

Gel Electrophoresis

Light Microscopy

Animal Handling (IACUC certified)

Proficiency in Microsoft Office & Windows, JMP Pro

INTERPERSONAL SKILLS

Fast learner

Excellent attention to detail

Collaborative team member

Leadership ability

Strong written & verbal communication skills

Capable of multitasking and handling prioritization

EDUCATION

MASTER OF SCIENCE Biology

Villanova University

Villanova, PA 2018

Coursework in different Cell and Molecular Biology topics with emphasis on Immunology

Recipient of Research Fellowship in summer 2016

Masters Thesis titled “Investigate the role of Lipid raft-based Membrane Order in CD4+ T cell responses”

CD4+ T lymphocytes are critical for orchestrating adaptive immune response against pathogens. Each CD4+ T cell through its antigen receptor (also known as “T cell receptor αβ”), recognize a protein component of the pathogen as foreign, and responds by a process dubbed as “T cell activation”. The early events of “T cell activation” are initiated within the microenvironment of dynamic and co-existing liquid ordered (Io) and disordered (Id) phases of the plasma membrane. Lipid rafts, which are the compositionally heterogeneous, nanometer size membrane domains enriched in saturated lipids and cholesterol, contribute to the ordered phase (Io) of the membrane. More so, phase-conversion from Io to Id results in loss of antigen-specific CD4+ T cell activation and cell division. I hypothesized that membrane order and proliferative capacity in CD4+ T cells will be restored by inserting cholesterol into the membrane. To address my hypothesis, I developed an

experimental system for controlled reconstitution of membrane order by inserting cholesterol into the plasma membranes after disrupting their Io phase. Membrane disorder (disruption of Io) was generated by graded incorporation of 7-ketocholesterol into the plasma membrane of lymph node cells of DO11 T cell receptor transgenic mice and membrane anisotropy was assessed after incorporation of 4-Di-ANEPPDHQ fluorescent dye followed by flow cytometry. DO11 TCRαβ transgene is specific to chicken-Ovalbumin 323-339 peptide when presented by a syngeneic (same genetic background) antigen presenting cells expressing I-Ad (an MHC class II protein) to assess T cell proliferation. Reconstituting lipid raft-based membrane order with cholesterol significantly (p < 0.05) reconstituted antigen-specific CD4+ T cell proliferation.

MASTER OF SCIENCE Microbiology

Peoples' Education Society of Applied Science

Bangalore, INDIA 2005

Among top three students in the University

Recipient of Scholarship on merit basis for continuing coursework

Participate in poster presentation viz. 'Hypersensitivity and Allergic Reactions' at IISC, India

Successfully completed six months’ internship on Medical Microbiology, KIMS Hospital, India

BACHELOR OF SCIENCE Microbiology

Peoples' Education Society of Applied Science

Bangalore, INDIA 2003

Among top three students in Bangalore University

Graduated with Honors

ACCOMPLISHMENTS

Winner of “Dow Everyday Award” in December 2017

Presented thesis-level research at annual Sigma XI poster session at Villanova University

Judge in Carver Science Fair, hosted by University of Pennsylvania

Member of Graduate Ambassador Program helping in open house, new student orientation

Member of International Student Association

Selected as an Examiner for the Microbiology in April 2009 by West Bengal State Council of Technical Education (WBSCTE) Best Lecturer award, 2011-12

WORK AUTHORIZATION

Authorized to work in USA

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