Da Shi, Ph.D

***** **** ****** **.

Germantown, MD 20874

Email: ac7fj7@r.postjobfree.com

Phone: 301-***-****

Work History

August 2018-current: Post-doctoral fellow with Dr. Marina Drobrovolskaia at National Cancer Institute, Immunology section of Nanotechnology Characterization Laboratory (Frederick, MD). Sterile preparation of human whole blood and isolation of peripheral blood mononuclear cell culture to induce inflammatory cytokines, chemokines, and interferons in vitro in accordance with approved methods and procedures. Performed Enzyme-linked Immunosorbent Assay (ELISA) to measure IL-8 as well as multi-array cytokine studies measuring interferons and from culture supernatants. Performed plasma coagulation tests (PT, APTT, and Thrombin) and analysis of complement activation from human whole blood in accordance with approved methods and procedures. Designed and performed experiments for flow cytometry to differentiate immune cell types from human whole blood in accordance with approved methods and procedures. The data from these experiments were collected, organized and recorded in a form suitable for publication. The experimental results were also reviewed for reliability and compared with data from similar experiments. Discussed scope and objectives of the research project with the research scientist regarding immune response from nanoparticles. Performed statistical analysis of data using Prism graphing software. Maintained awareness of current research in field of immunological response to lipid-based nanoparticles. Gained and kept Public Trust Clearance. Adhered to patient privacy policy to protect patient identifying information from blood donors. Adhered to blood-borne pathogen safety protocols when working with human blood.

July 2017- August 2018: Oak Ridge Institute for Science Education Post-doctoral Fellow with Dr. William Vogt. Food & Drug Administration in the Division of Biomedical Physics of the Center for Radiological Devices and Health (Silver Spring, MD).

Detailed characterization of gold nanoparticles in tissue relevant phantoms and following biodistribution in a mouse tumor model using inductively coupled plasma mass spectroscopy (ICPMS), photoacoustic imaging

(PAI) and fluorescent In Vivo Imaging System (IVIS) in accordance with approved methods and procedures. Performed routine equipment maintenance for ICPMS and PAI. Sterile technique used in culturing of breast cancer cell line and during inoculation of tumor in nude mice. The data from these experiments were collected, organized and recorded in a form suitable for publication. The experimental results were critically analyzed to determine whether data generated were accurate and valid in formal presentation at weekly progress meetings. The experimental results were also reviewed for reliability and compared with data from similar experiments. Discussed scope and objectives of the research project with the research scientist regarding biodistribution of gold nanoparticles and imaging with PAI. Performed statistical analysis of data using Microsoft Excel and MATLAB software. Maintained awareness of current research in gold nanoparticle biodistribution and xenograft tumor animal models. Advised scientist of the methods to be used and estimated time and resources required for animal studies related to xenograft tumor generation, ICPMS, PAI and IVIS. Collaborated with investigators and fellows from engineering, chemical, biological, and veterinarian backgrounds as required to progress with research project. Amended animal safety protocol and gave lab tours for Institutional Animal Care and Use Committee (IACUC) inspections. Coordinated activities with the veterinary and animal care staff in BSL2 environment. Gained and kept Public Trust Clearance. 2011-2017 Graduate Research Assistant for Dr. Rao Gullapalli, University of Maryland, Baltimore Department of Biochemistry and Molecular Biology (Baltimore, MD). Applied scientific principles, theories, practices and methodology to my research on brain development in the mouse model of Fragile X syndrome (FXS), a genetic mutation of FMR1 gene, using advanced imaging techniques with magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) to determine brain metabolite levels. Performed experimental drug treatment, western blot and PCR for genotyping on mice in accordance with approved methods and procedures. The data from these experiments were collected, organized and recorded in a form suitable for publication. The experimental results were critically analyzed to determine whether data generated were accurate and valid with scientists and in formal presentation at internal and external meetings. The experimental results were also reviewed for reliability and compared with data from similar experiments. Discussed scope and objectives of the research project with the research scientist regarding brain developmental trends and differences following treatment, if any. Performed statistical analysis of data using SPSS and Microsoft Excel software. Maintained awareness of current research in FXS with respect to pathology, MRI studies and interpretations, and potential preclinical treatments. Advised scientist of the methods to be used and estimated time and resources required for animal studies related to generation of animals and performing MRI at different time points. Wrote and amended animal safety protocol and gave lab tours for Institutional Animal Care and Use Committee (IACUC) inspections. Coordinated activities with the veterinary and animal care staff in BSL1 environment. As part of my graduate support work, I assisted my lab in operation of 3T Clinical MRI to image research patients for scientific studies. Patient work involved explaining MRI operations, preparation for MRI, answering questions and providing guidance to keep patient comfortable while in the MRI. Maintained strong communications between my lab and that of collaborating investigators regarding patient progress and data. Kept patient data confidential.

2009-2011 Research Assistant for Dr. Rao Gullapalli, University of Maryland, Baltimore Department of Diagnostic Radiology and Nuclear Medicine (Baltimore, MD). Operated a small animal 7 Tesla MRI as part of a core university service, studying brain and muscle injury and pathology in rodent models, in accordance with approved methods and procedures. Duties included image quality assurance measured by signal to noise ratio in experimental subjects and in phantoms, and performing maintenance of MRI hardware in accordance with approved methods and procedures. The data from these experiments were collected, organized and recorded in a form suitable for publication. The experimental results were critically analyzed to determine whether data generated were accurate and valid with scientists and in formal presentation at internal and external meetings. The experimental results were also reviewed for reliability and compared with data from similar experiments. Discussed scope and objectives of the research project with the research scientist regarding traumatic brain injury animal models. Maintained awareness of research in field of traumatic brain injury, MRI studies and interpretations. Advised scientist of the methods to be used and estimated time and resources required for animal studies in performing MRI. 2007-2009 Research Assistant for Dr. Mary McKenna, University of Maryland, Baltimore Department of Pediatrics (Baltimore, MD).

Performed animal surgery to produce rodent model of hypoxia-ischemia injury and extraction of brain metabolites in accordance with approved methods and procedures. The data from these experiments were collected, organized and recorded in a form suitable for publication. The experimental results were critically analyzed to determine whether data generated were accurate and valid with scientists and in formal presentation at internal and external meetings. The experimental results were also reviewed for reliability and compared with data from similar experiments. Advised scientist of the methods to be used and estimated time and resources required for generating hypoxic-ischemic animals. Gave lab tours for Institutional Animal Care and Use Committee (IACUC) inspections. Coordinated activities with the veterinary and animal care staff in BSL1 and BSL2 environments.

Education

• Doctor of Philosophy (2011-2017): Biochemistry and Molecular Biology at University of Maryland, Baltimore

• Bachelor of Science (2003-2007): Biological Sciences with Biopsychology minor at University of Maryland, Baltimore County

Relevant Graduate Coursework

Mechanisms in Biomedical Sciences

Advanced Molecular Biology

Advanced Biochemistry

Introduction to Biomedical Chemistry

Molecular Mechanisms of Signal Transduction

Biostatistical Methods

Introduction to Clinical and Translational Research Techniques and Experiences

Magnetic resonance imaging (2011-2017):

o Operating magnetic resonance imaging scanners, Bruker 7 Tesla small animal magnet (mice, rats and guinea pigs) and Siemens 3T clinical magnet

o Understanding of specialized MR protocols, including diffusion tensor imaging, magnetization transfer imaging, functional MRI, and 1H spectroscopy

o MRI Software: FSL software for diffusion and fMRI analysis, Trackvis for diffusion tractography, o Medical Imaging Processing, Analysis, and Visualization, and ImageJ for image processing Inductively coupled plasma mass spectrometry in mouse organs to measure gold concentration (2017-2018) o Operation of Thermo Series II ICPMS

o Digestion of organ samples following National Institute of Standards and Technology protocol In Vivo Imaging System (IVIS) 200 & Spectrum operation to measure fluorescent intensity in mice

(2017-2018)

Photoacoustic imaging of in vivo and ex vivo animal models (2017-2018) Ultrasound imaging of in vivo animal models (2017-2018) Gold nanoparticle evaluation using spectrophotometer (2017-2018) Fluorophore evaluation using fluorimeter (2017-2018) Animal surgeries/techniques (2007-2018):

o Right carotid artery ligation in neonatal rat pups for hypoxic/ischemic encephalopathy model o Cortical concussion model of traumatic brain injury in young and adult rats o Tumor xenograft in nude mice from cultured MDA-MB-231 breast cancer cells o Animal handing experience (11 years)

o Injections: Subcutaneous, intra-peritoneal (IP), & intravenous through the tail vein o Mouse organ harvesting

o Induction of anesthesia through injections (IP) or inhalant (isoflurane vaporizer) o Comfortable working in animal biosafety level (ABSL) 1 (2007-2017) & 2 (2008-2009 & 2017-2018) Cellular (2011-2018):

o Aseptic technique for cell culturing

o Mechanical stretch injury of cultured neurons

o Culture of passaged and primary neurons, MDA-MB-231 breast cancer line o Isolation of myelin and synaptosomes through differential centrifugation o Preparation of human blood and isolation of peripheral blood mononuclear cell cultures o PCR for genotyping and collecting tail samples

o Western Blot

o Enzyme-linked immunosorbent Assay (ELISA)

o Flow cytometry using BD FACSCaliber and NovoCyte NovoSampler Pro Pathology (2007-2017):

o Rodent fixation left ventricle perfusion

o Operating a cryostat

o Histological processing

o Bioquant Image Analysis software

SPSS statistics software (2011-2017)

Reading and limited writing with MATLAB code (2011-2017) Animal Behavior (2009-2017):

o Open field maze and Morris water maze

o Social interaction

o Novel object recognition

o Beam walking

o Attentional set shifting task

Other skills (2007-2018)

o Writing and amending Animal Study Protocols

o Laser safety training

o Magnetic Resonance Imaging safety training

Peer-reviewed Publications in chronological order: 1. Xu S, Shi D, Pratt SJ, Zhu W, Marshall A, Lovering RM. Abnormalities in brain structure and biochemistry associated with mdx mice measured by in vivo MRI and high resolution localized (1)H MRS. Neuromuscul Disord. 2015;25(10):764-72.

2. Xu S, Waddell J, Zhu W, Shi D, Marshall AD, McKenna MC, Gullapalli RP. In vivo longitudinal proton magnetic resonance spectroscopy on neonatal hypoxic-ischemic rat brain injury: Neuroprotective effects of acetyl-L-carnitine. Magn Reson Med. 2015;74(6):1530-42. 3. Raufman JP, Xu S, Cheng K, Khurana S, Vivian D, Shi D, Gullapalli R, Polli J. Phrygian cap appearance of a mouse gallbladder on magnetic resonance imaging. J Vet Sci Med Diagn. 2013; 28;2(1). 4. Shi D, Xu S, Waddell J, Scafidi S, Roys S, Gullapalli R, McKenna MC. Longitudinal in vivo developmental changes of metabolites in the hippocampus of Fmr1 knockout mice. J Neurochem. 2012; 123(6):971-81.

5. McMillan AB, Shi D, Pratt SJ, Lovering RM. Diffusion tensor MRI to assess damage in healthy and dystrophic skeletal muscle after lengthening contractions. J Biomed Biotechnol. 2011;2011:970726. 6. Raufman JP, Xu S, Cheng K, Khurana S, Johnson D, Shao C, Kane MA, Shi D, Gullapalli R, Polli J. In vivo magnetic resonance imaging to detect biliary excretion of 19F-labeled drug in mice. Drug Metab Dispos. 2011; 39(5):736-9.

7. Xu S, Zhuo J, Racz J, Shi D, Roys S, Fiskum G, Gullapalli R. Early microstructural and metabolic changes following controlled cortical impact injury in rat: magnetic resonance imaging and spectroscopy study. J Neurotrauma. 2011;(10):2091-102.

8. Scafidi S, Fiskum G, Lindauer SL, Bamford P, Shi D, Hopkins I, McKenna MC. Metabolism of acetyl- Lcarnitine for energy and neurotransmitter synthesis in the immature rat brain. J Neurochem. 2010; 114(3):820-31.

Memberships in Professional Societies

International Society of Magnetic Resonance in Medicine (ISMRM) American Society for Neurochemistry (ASN)

Professional Honors

President's Student Leadership Institute in Career Development 2016 Graduate Student Association Research Award. Spring 2014 ISMRM Magna Cum Laude Merit Award. May 2013

Trainee stipend award from ISMRM. May 2013, 2014, 2015 Travel award for International Society for Neurochemistry Advanced School on New Approaches in Glial Cell Research in Barcelona, Spain. August 2012 Invited speaker 43rd meeting of ASN Baltimore, MD. March 2012 Volunteer work

Mentoring high school student at Paul's Place, Baltimore, MD 2013-2016 o Assisting with homework

o Help preparation for college

Providing food to at-risk population with Project Jump Start, Baltimore, MD 2009-2016 o Leadership role to guide and recruit volunteers

o Student government representative

o Organized fundraisers and petitioned organizations for support References

Dr. Marina Drobrovolskaia, Senior Principal Scientist Phone: 301-***-**** Email: ac7fj7@r.postjobfree.com

Dr. William Vogt, Staff Fellow

Phone: 240-***-**** Email:ac7fj7@r.postjobfree.com Dr. Rao Gullapalli, Professor and mentor

Phone: 410-***-**** Email: ac7fj7@r.postjobfree.com

Dr. Mary McKenna, Professor and co-mentor

Phone: 410-***-**** Email: ac7fj7@r.postjobfree.com Dr. Su Xu, Associate Professor

Phone: 410-***-**** Email: ac7fj7@r.postjobfree.com

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