Anantha Marisetty, PhD

713-***-**** ac7d5t@r.postjobfree.com

SUMMARY

Expertise in understanding key signaling pathways and how alteration in gene and microRNA expression contribute to cancer

Extensive experience working in vivo with Orthotopic, GEM and xenograft tumor models of brain and blood cancers

10 years’ working experience in vitro and in vivo biological assays for validation of small molecule compounds and therapeutic drug targets in Cancer Stem cells resulting in 5 high impact publications

Strong experience in multiple biological disciplines including Cancer Biology, Molecular Biology, Microbiology, Biochemistry, Immunology, Genetics and Mouse models

EDUCATION

Doctor of Philosophy in Cancer Biology, University of Texas MD Anderson Cancer Center, Houston 2015

Thesis-REST-mediated regulatory circuit controls oncogenic properties of Glioblastoma stem cells

Master of Science in Biotechnology, University of Massachusetts, Lowell 2009

Thesis –Novel Roles for Ca2+/Calmodulin-dependent protein kinases in regulating blood myeloid cell growth & development

Bachelors in Biotechnology, Bapatla Engineering College (BEC), India 2006

Thesis- Identification of pure commercial in MECH162 and RCH2 BT cotton hybrids by qualitative and quantitative analysis of cry1Ac gene

PUBLICATIONS

Konrad Gabrusiewicz, Xu Li, Jun Wei, Yuuri Hashimoto, Anantha Marisetty, Martina Ott, et al. Glioblastoma stem cell-derived exosomes induce M2 macrophages and PD-L1 expression on human monocytes. (Oncoimmunology, December 2017)

Anantha Marisetty, Sanjay Singh, Tran Nguyen, et al. REST represses miR-124 and miR-203 to regulate distinct oncogenic properties of glioblastoma stem cells. (Neurooncology, August 2016)

Anantha Marisetty, Pratheesh Sathyan, Pascal O. Zinn, et al. Mir-21–Sox2 Axis Delineates Glioblastoma Subtypes with Prognostic Impact (Journal of Neuroscience, Co-first author, November 2015)

Singh SK, Anantha Marisetty, Sathyan P, et al. REST-miR-21- SOX2 axis maintains pluripotency in E14Tg2a.4 embryonic stem cells. (Stem Cell Research, September 2015)

Mohamed M. Kamal, Pratheesh Sathyan, Sanjay K. Singh, Pascal O. Zinn, Anantha Marisetty et al. REST Regulates Oncogenic Properties of Glioblastoma Stem Cells. (Stem cells, December 2011)

Peter Gaines, James Lamoureux, Anantha Marisetty et al. A cascade of Ca2+/calmodulin-dependent protein kinases regulates the differentiation and functional activation of murine neutrophils. (Experimental Hematology, April 2008)

WORK EXPERIENCE

Post-doctoral fellow in Dr. Amy Heimberger’s Lab 2015-Present

MD Anderson Cancer Center

Investigating the genes and microRNAs regulating immune suppression and activation in Glioblastoma Multiforme

Graduate Research Assistant in Dr. Sadhan Majumder’s Lab 2009-2015

The Graduate School of Biomedical Sciences (MD Anderson Cancer Center)

Successfully studied the role of repressor transcriptional factor RE1 silencing factor (REST) in glioblastoma stem cells and the mechanism by which it regulates oncogenic properties

Identified a new mechanism in GBM (miR-21-Sox2 axis) that can classify 50% of patients into two subtypes with distinct molecular, radiological, and pathological characteristics

Studied the role of REST-miR-21-Sox2 axis in maintaining the pluripotency in E14Tg2a.4 embryonic stem cells (Stem Cell Research)

Established a MRI-T2/FLAIR imaging model to study GBM

Graduate Research Assistant in Dr. Peter Gaines’s Lab 2007-2009

The University of Massachusetts, Lowell, MA

Validated several Calcium kinase inhibitors and studied their role in neutrophil proliferation and functional activation (Experimental Hematology)

Extensively studied the gene expression profile of primary bone marrow cells

Research Intern with Dr. Christina Ferrick 2009-2009

Acambis Inc, a subsidiary of Sanofi Pasteur, Cambridge, MA

Involved in a study of humoral response to a lead vaccine candidate

Teaching Assistant- Cell culture Laboratory 2009-2009

The University of Massachusetts, Lowell, MA

Aiding recitation for Cell culture laboratory

Supervise graduate and undergraduate laboratory sessions

Grade the laboratory sessions and exams for both graduate and undergraduate students

Teaching Assistant-Brain Tumors Meeting, The cold spring laboratories, NY 2014 -2014

Design the curriculum, invite speakers and lead discussions in the class room

AWARDS AND ACHIEVEMENTS

AACR travel award by Proteintech, Washington DC April 2017

First Author paper is featured in Neurooncology as highlights from literature March-2016

Oral presentation award at the Cancer Biology Program Retreat – April 2014

Oral Finalist at the Genes & Development program Retreat - March 2014

GSBS travel award and Cancer Biology Travel Award – March 2014

Poster Finalist for Trainee Research Day 2013 &2012, MD Anderson Cancer Center- June 2013 & June 2012

Awarded the Graduate Research Fellowship by UT MD Anderson Cancer Center 2009-2011

Finalist for the Steven R. Williams Award for Excellence in Graduate Research, UML - April 2008

Received T.Venugopal Rao Memorial Award for the best outgoing student for the academic years 2002-2006

Secured an overall second rank during Under graduation at Nagarjuna University -2006

INVITED TALKS & PRESENTATIONS

Elucidation Of MicroRNA-osteopontin Circuit In Glioblastoma Associated Infiltrating Macrophages Society for NeuroOncology-2017 (Poster Presentation)

MicroRNA-Osteopontin circuit as a therapeutic target for Glioblastoma Multiforme Annual Brain Tumor Center Retreat -2017 (Poster Presentation)

REST-mediated regulatory circuit controls oncogenic properties of Glioblastoma stem cells Cancer Biology Retreat and Genes and Development Retreat - 2014 (Oral Presentation)

REST-mediated regulatory circuit controls oncogenic properties of Glioblastoma stem cells, Cold Spring Harbor Brain tumors meeting, NY. Jul 2012 and Cancer Biology Program Retreat 2012. (Oral presentation)

REST-mediated regulatory circuit controls oncogenic properties of Glioblastoma stem cells, Cancer Biology Program Retreat 2012 and Genes and Development Program Retreat 2012 (Poster presentation)

Regulation of c-Myc protein stability by Rak tyrosine kinase, Cancer Biology Program Retreat 2011 (Poster presentation)

Presented a poster at the Eastern New England Biological conference and UML student research symposium held at University of Massachusetts, Lowell-April 2009

Presented a poster at the 11th Annual UML Student Research Symposium at University of Massachusetts Lowell - April 2008.

Presented a poster at The 66th Eastern New England Biological conference held at Colby-Sawyer College, New London, New Hampshire- April 2008

Presented a paper titled “Identification of pure commercial BT cotton hybrids” in BIOGEN, a technical symposium held at Bapatla Engineering College in Jan 2006

LABORATORY SKILLS AND TECHNICAL EXPERTISE

Cell Culture: Routine passage of adherent & suspension cell lines, culturing stem cells (from patient tumors), Cryogenics, Transient & Stable transfection & viral transduction of mammalian cell lines, Cell based assays, Mamosphere assays, Flow Cytometry, Clonogenic assays, migration and invasion assays, Neutrophil functional assays like respiratory burst, Chemotaxis, Under Agarose assays and growth responses. Self-Renewal assays, Brdu Incorporation/Proliferation Assays, Luciferase Assays.

Molecular Biology: Nucleic acid isolation from bacterial, plant & mammalian cells, Bacterial transformation, Gene cloning, Sequence analysis, Restriction digestion, Agarose gel Electrophoresis, site directed mutagenesis, EMSA, Viral production & Titer Estimation, CRISPR/CAs9 Technology, Chromatin Immunoprecipitation, Northern hybridization with radioactively labeled P32, Southern Blotting, microRNA microarray and mRNA microarray analysis.

Genetic purity analysis of hybrid crop plants using SSR & ISSR markers.

Gene Expression Analysis: Polymerase chain reaction, Reverse transcriptase PCR, Quantitative PCR (q-PCR), DNA sequencing, protein expression profiles by western blot analysis, Immunoprecipitation, Microarray data analysis using R.

Immunology: Blood grouping, Immunoelectrophoresis, ELISA, ELISPOT, Widal test, Immunofluorescence, Immunocytochemistry, Live mice handling, Hybridoma cell production, Extraction of Blood, Spleen, Bone marrow & thymus cells & culturing, Isolation of CD11b+ cells and skewing monocytes.

Mouse models:

Genotyping, mouse colony maintenance, Murine tissue Processing, Isolation and culture of neural stem cells and cerebellar progenitor cells from the mouse brain, orthotropic brain tumor model (injecting patient derived tumor cells into nude mice and studying the gene signatures), intraperitoneal injections for treatment, Live animal imaging to monitor tumor volume

Programming Languages& Application Packages: C, C++, Basic Bioinformatics Tools, data Analysis using Prism software, MS Office, Graph Pad prism

MANAGEMENT & COMMUNICATION

Excellent oral presentation and communication skills

9+ years’ experience in designing, executing, troubleshooting and managing research projects

Lead and collaborated with team of scientists in the field of oncology resulting in 2 first author and 3 high impact publications

Experienced in training junior scientist’s (2 undergraduate researchers, 3 graduate students and a postdoctoral fellow)

JUDGING & ORGANIZATIONS

Judge for the GSBS Research day presentation June 2016/2017

Member of National Post-doctoral association

Active member at the Texas Life science Forum and Rice Business plan competition 2016

Actively volunteered in the annual convention of the Andhra Pradesh Academy of Sciences (APAS) conducted in Bapatla Engineering College India 2005

Organized various national level symposiums like BECTAGON & BIOGEN 2004-2006

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