Assistant Research

RESUME

Kevin M. Patrie

***** ******* **** **.

Macomb, MI 48042

Phone: 248-***-****

E-mail: **********@*****.***

Education:

**** **.*., *********, ********, and Developmental Biology Program, The Ohio State University, Columbus, OH

Major interests: Signal Transduction, Growth Factors/Growth Factor Receptors

GPA - 3.6/4.0

1988 B.S., General Biology, Central Michigan University, Mt. Pleasant, MI

Major - Biology

Minor - Chemistry

GPA - 3.4/4.0

Professional Experience:

07/18 present Logistics Material Handler, WRAL, Warren, MI

Secondary supervisor; label, band and wrap pallets of automotive parts prior to shipping to assembly plants; verify outgoing and incoming shipments; inspect and possibly rework parts when required.

06/17 11/17 Patent Analyst, CPA Global, Southfield, MI

Researched prior art documents to reject claims of patents under review by the United States Patent and Trademark Office (USPTO).

Provided written opinions based on prior art documentation to reject or allow claims on patents being reviewed by the USPTO.

2010 2016 Director of Research, Oxford Biomedical Research, Rochester Hills, MI

In addition to the responsibilities and duties as a Senior Scientist at Oxford Biomedical Research (see below):

1.Manage the day-to-day operations associated with Research and Development (R & D) including prioritizing projects and directing technicians performing experiments associated with R & D efforts.

2.Coordinate marketing strategies with research and development for new and existing products and assays.

3.Coordinate the transition of products and assays from R & D to Production.

4.Develop Standard Operating Procedures (SOPS) for product development and help establish Product Manufacturing Procedures (PMPs).

5.Assist in managing the day-to-day operations of all other aspects of the company.

2009 2010 Senior Scientist, Oxford Biomedical Research, Inc., Rochester Hills, MI

Perform molecular biology techniques for the expression of proteins as part of company’s product portfolio and as components in current and future company immunoassays. This includes, but is not limited to, PCR amplification and cloning of target cDNAs into expression vectors, expressing proteins from the cDNAs in either prokaryotic or eukaryotic expression hosts and purification of expressed proteins, Western analysis, SDS-PAGE analysis (silver and conventional Coomassie Blue staining of gels). When required, perform biochemical activity assays on expressed protein emzymes to attain specific activities.

Expert in mammalian cell culture techniques (aseptic) for both adherent and suspension cells. Adept in insect cell and bacterial expression systems for heterologous expression of proteins.

Develop immunoassays that will become part of the company’s product line. Procedures utilized include: purification of monoclonal and polyclonal antibodies (ammonium sulfate fractionation, Protein A or G and antigen or peptide affinity chromatography); evaluating and optimizing conditions for antigen-down EIAs, conventional sandwich ELISAs; competitive ELISAs.

Conceptualize and write SBIR grant applications and execute/supervise the experiments associated with funded grant awards. Prepare written updates and final reports associated with funded grant proposals.

2008 09 Scientist, Proteos, Inc., Kalamazoo, MI

Expression of proteins from a number of systems including mammalian (CHO and HEK293 cells), insect (High5 and Sf9), yeast (Pichia pastoris) and E. coli.

Involved in various stages of the expression of proteins for customers including, but not limited to, cloning of cDNAs into expression vectors, transfection of mammalian and insect cells, transformation of E. coli and yeast.

Small scale pilot expression studies of target proteins (50-100 milliliters) to scale up expression (10-50 liters).

Isolation and purification of expressed proteins (both secreted and cellular) using IMAC technology.

2006 08 Research Lab Specialist Senior, Human Genetics Department, University of Michigan, Ann Arbor, MI

Involved in research on a small number of projects focused on the identification of the genomic locus responsible for a spontaneous mouse mutation. Conventional positional cloning was being utilized for this purpose.

The mouse mutant provided an outstanding tool to determine early genetic controls over body pattern and limb field specification involved in mammalian limb development.

Duties include maintaining the mutant line on an inbred mouse strain and testing mice that are recombinant at markers near the genetic locus in question by PCR-based genotyping methods (e.g. SNP analysis). Other methods used included Southern blotting, mouse husbandry and maintenance and non-recoverable mice surgeries for the analysis of embryonic development.

In addition, other standard molecular biology and biochemical techniques were employed as well as bioinformatics.

2002 06 Research Assistant Professor, Internal Medicine Department, University of Michigan, Ann Arbor, MI

Independent research focused on identifying functions of the molecular scaffolding protein MAGI-1 and its protein family member MAGI-2 within the context of the epithelial cells that express them.

Analysis of epithelial cell biology was emphasized with particular attention given to the visceral epithelial cells, or podocytes, of the kidney glomerulus, where personal published data suggested that MAGI-2 might associate with the actin cytoskeleton in these cells thereby implicating this protein in regulating actin dynamics.

Identified proteins that interacted with MAGI-1 and -2 and assessed the biological significance of those interactions.

Performed in vitro binding assays using fusion proteins, immunoprecipitations, and immunofluorescence to verify interactions as well as establish the proper localization and function of these MAGI-1 and-2 interacting proteins.

Utilized small interfering RNA (siRNA) technology to “knockdown” the expression of MAGI-1 in MDCK cells with the aim of elucidating its function in the context of this epithelial cell; applied the same technology to “knockdown” MAGI-2 expression specifically in the podocytes of mouse kidneys.

Routinely employed enzyme immunoassays (EIAs) assays to semi-quantitatively measure protein interactions as well as to test potentially useful monoclonal antibodies used in these studies.

1998 2002 Research Fellow, Internal Medicine Department, University of Michigan, Ann Arbor, MI

Initial characterization of proteins that interact with the membrane-associated protein MAGI-1 and training in the molecular structure/function of visceral epithelial cells (podocytes) of the renal glomerulus.

Personal research project involved the assessment of MAGI-1 localization and function in a cell culture model (MDCK cells) and mouse kidneys.

Employed GST fusion proteins to investigate the protein-protein interactions of potential binding partners of MAGI-1 as well as to determine the regions of MAGI-1 required for its proper localization and function.

To help elucidate the function of MAGI-1, a high throughput screen for epithelial- and glomerular-specific binding proteins was conducted using an expression cloning technique on cDNA libraries.

Characterization of the MAGI-1 interacting proteins was performed using Western blotting, ligand blot overlay (FarWestern) analyses and immunofluorescence on stable MDCK cell lines and kidney tissue sections.

1997 98 Postdoctoral Fellow, Cell Biology, Neurobiology and Anatomy Department, University of Cincinnati, Cincinnati, OH

During my first year as a Postdoctoral Fellow I was involved in a project addressing the involvement of particular cells within a peripheral nerve in benign lesions of neurofibromatosis type I. For this our lab was using a mouse knockout model for the neurofibromatosis type I gene (NF1).

Conducted reciprocal sciatic nerve graft transplants between animals that are homozygous and heterozygous for the NF1 gene.

I have therefore become quite proficient at performing surgeries on the animals. I was also involved in the maintenance of our transgenic mouse colony (this requires setting up matings, ear punching, keeping records of the animals, and genotyping the animals using PCR technology). In addition, I have obtained experience in tissue fixation methods, tissue sectioning (both frozen sections and paraffin embedded tissue), and immunohistochemistry techniques.

Professional Affiliations:

2002 2015 American Association for the Advancement of Science

2002 2007 American Society for Cell Biology

Authored Publications/Technical Writing (Grants, journal articles, poster abstracts, etc.)

Available upon request

References:

Available upon request

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