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strong Molecular Technologist candidate

Location:
Bloomington, IN
Salary:
34000
Posted:
December 28, 2018

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Resume:

Lu Ruan

Email: ac72jv@r.postjobfree.com Phone: +1-812-***-****

LinkedIn: www.linkedin.com/in/lu-ruan-96026536

Education

M.S. in Molecular and Cellular Biochemistry, GPA 3.5, Indiana University, Bloomington, 2018

B.S. in Chemical Biology, GPA 3.6, Xiamen University, Xiamen, China, 2012

Skills and Research Techniques

Software: Origin, ChemOffice, Pymol, Chimera, Graphpad Prism, Lab Archive and other lab notebook software, MS Office.

Techniques: Molecular Cloning, Protein Purification, Regular PCR and qPCR, Cell Culture, ELISA Assays, Cell-based Assays, Tissue DNA/RNA extraction, Western Blot, Southern Blot, Infection Assay, Light Microscopy, Transmission Electron Microscopy, Chromatography (FPLC, HPLC), Light Scattering, Protein-Ligand Binding Assays (MST), Bio-crosslinking Assay.

Research and Professional Experience

2015.08 – 2018.03 Graduate Student Researcher

Dr. Adam Zlotnick Lab, Indiana University Bloomington, Indiana

Independently designed, purified and characterized a series of Hepatitis B Virus core protein mutants to study antiviral resistance, confirmed a protein mutant has stronger resistance to a core protein directed antivirals.

Applied in vitro biophysical assays (fluorescence, UV/vis, light scattering, size exclusion) to study assembly kinetics and thermodynamics, confirmed hydrophobicity of HBV core protein residue T109 plays an important role in capsid assembly.

Used cell-based transfection system (Hep AD38 cell line) to study core directed antiviral effect and cyto-toxicity with ELISA Assays.

Monitored capsid assembly dynamics in support of nano fluidic experiment with collaborator Lab.

2014.01 – 2015.07 Research Assistant

Dr. Lingling Chen Lab, Indiana University Bloomington, Bloomington, Indiana

Independently designed and purified Chlamydial GroEL1 and GroES chaperonines complex, confirmed chlamydial GroEL1 served as the main chaperonine in chlamydia.

Gained expertise on chaperonine activity of Chlamydial GroEL1/GroES complex via protein refolding assay and ATP binding assay, identified Proline 35 of Chlamydial GroES will affect ATP binding activity and ATPase activity.

2010.07 – 2012.05 Undergraduate Student Researcher

Dr. Donghai Lin Lab, Xiamen University, Xiamen, China

Expressed and purified Raf1 Kinase and Raf1 kinase inhibitor protein (protein).

Tested protein interactions between Raf1 and RKIP via pull-down experiment and other biochemical assays, confirmed weak to medium strength interaction between Raf1 1-147 segment and RKIP by titration assay.

Awarded by Yumiao Undergraduate Scientific Research Fund of the college (4 of 60)

Internships and Working Experience

2012.9 – 2013.5 Research Assistant, RiboBio Co., Guangzhou, China

Assisted senior scientists to develop a miRNA silencing assay on multiple lung cancer cell lines.

Maintained and kept record of multiple lung cancer cell lines (h1299, A549, nci-h460) for cell viability assay.

Extracted RNA from clinical tissue samples.

2011.7 - 2011.8 Intern, Xiamen Food and Drug Administration, Xiamen, Fujian, China

Assisted setting up the examination standards for Goupi Gao (a traditional Chinese medicine) according to GLP and Pharmacopoeia of the People's Republic of China,

Characterized Borneol in Goupi Gao with thin layer chromatography.

Confirmed and reviewed experimental results of sample data sheets and filled in QC reports.

2009.7 – 2009.8 Intern, Guangdong Institute of Microbiology, Chinese Academy of Sciences

Participated in a project on antibacterial effect of nano-silver particles,

Measured bacterial growth curve under different treatment conditions,

Analyzed size distribution and morphology of nano-silver particles with softwares.

Publications

1)L. Ruan and A. Zlotnick (2018) Resistance of the HBV Capsid to Assembly-directed Antivirals Correlates with Capsid Stability. (J Virol. 2018 Sep 26;92(20))

2)P. Kondulis, L. Ruan, A.Zlotnick and S. Jacobson (2018) High Dynamic Range Resistive-

Pulse Detection on Nanopore Sensing Regions Connected in Parallel.(In preparation)

3)Illingworth, M., A. J. Hooppaw, L. Ruan, D. J. Fisher and L. Chen (2017). "Biochemical and Genetic Analysis of the Chlamydia GroEL Chaperonins." Journal of Bacteriology 199(12).

4)Wu, Z., C. Fu, L. Shi, L. Ruan, D. Lin and C. Guo (2014). "Structural basis for RKIP binding with its substrate Raf1 kinase." Biotechnology Letter 36(9): 1869-1874.

5)Zheng, F, F.; Ruan, L.; Liu, Y.; Zhao, Y, F.; Wu, X, J. (2011)“Recombinant Expression and Weak Interaction with GDP/ADP of hARF1” Journal of Xiamen University (Natural Science) Vol.50 No.6

Science Conferences and Presentations

1)XXV Biennial Conference on Phage/Virus Assembly, 2017

Resistance of the HBV Capsid to Assembly-directed Antivirals Correlates with Capsid Stability (Poster Presentation)

2)Indiana University Graduate Recruitment Weekend, 2014

Why Do Chlamyidae Have So Many Chaperonins? (Poster Presentation)

Teaching Experience

Associate Instructor, Department of Chemistry, Department of Biology, Indiana University

General Chemistry Lab (C127) Spring 2014

General Biology Lab (L113), Fall 2014

Microbiology Lab (M315) Summer 2014, Summer 2015

Led lab session and office hour independently, assisted students with experiments

Taught students to follow good working habits in lab

Graded assignments and quizzes and proctored Exam

Biotechnology Lab (T315), Fall 2015

Molecular Biology Lab (L323). Spring 2016

Prepared experimental materials for classes and assisted students with experiments

Gave lectures on Chembiodraw and PyMOL softwares

Guided students on academic paper reading and writing



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