Assistant Medical
Resume:
Srisha Sridharan Biotechnologist
******.****@*****.*** 732-***-**** Dallas, TX 75254
Detail-oriented researcher with eight years of experience in laboratory bench work and administration of biotechnology initiatives. Expertise conducting experiments in microbial genetics and genomics, analyzing data, conducting literature searches, and processing and publishing results. Proven track record of consistently delivering research on time and within budget and established protocols in collaboration with multidisciplinary teams.
Areas of Expertise include:
Molecular Lab Techniques
Health & Safety Regulatory Compliance
Embryo Harvesting, Microinjection
GCP Certified and GLP Trained
Mouse Establishment & Maintenance
Tissue Culture Analysis
Laboratory Inventory Management
Literature Review
Education
Master of Science in Biotechnology, May 2011
University of Texas at Dallas • Richardson, TX
Overall GPA:3.5/4.0
Bachelor of Technology in Biotechnology, June 2008
St. Joseph's College of Engineering (Anna University) • Chennai, India
Overall GPA: 3.5/4.0
Professional Experience
Biochemistry, UT Southwestern Medical Center • Dallas, TX • July 2014 to Present
Research Associate
Conduct research using genetic manipulation to create and provide transgenic (genetically altered) mice to investigators based on research needs and requests.
Experienced in embryo harvesting, embryo transfer and hormone injection for super-ovulation and Caesarean section / fostering of transgenic mice.
Key Accomplishments:
Effectively design research studies, generating transgenic mice by pronuclear and cytoplasmic cell injection using CRISPR-Cas9 system.
Conduct experiments with tissue culture for cell injection, growing cells in feeder-based media, performing electroporation and selection of cells, and expanding positive cells.
Manage mouse inventory, controlling and shipping mice following established protocols, preparing shipping containers and labels, and completing paperwork.
Perform lab maintenance as needed, managing inventory and safety records and maintaining lab stock.
Pediatrics, UT Southwestern Medical Center • Dallas, TX • August 2013 to July 2014
Research Associate
Conducted research into kidney disease by analyzing the combined role of uromodulin and mucin in urine samples. Extensive experience in setting up mouse lines (breeding, genotyping, dissection, PCR, Quantitative PCR, care and handling).
Key Accomplishments:
Evaluated effects of TRPM6 (magnesium channel) with respect to lower intestinal magnesium levels causing hypomagnesaemia with secondary hypocalcaemia (HSH) by site-directed mutagenesis.
Coordinated clinical human urine samples as a control group to test the presence of uromodulin, following ICH and GCP guidelines
Developed proficiency in multiple techniques for mice analysis, including co-immunoprecipitation, Western Blotting, ELISA,and other complex techniques.
Conducted literature searches, reviewed scientific literature and interpreted data.
Dept. of Molecular Genetics, UT Southwestern Medical Center • Dallas, TX• July 2011 to August 2013
Research Assistant
Studied action of Ghrelin (hunger stimulating hormone produced in stomach) in relation to obesity using mouse models, utilizing ELISA, PCR, genotyping, and other biochemistry techniques on manipulated mice. Work published in Journal of Biological Chemistry, 2012 (Profound hypoglycemia in starved, ghrelin-deficient mice is caused by decreased gluconeogenesis and reversed by lactate or fatty acids.)
Key Accomplishments:
Performed body weight and body fat analysis in mice fed with high fat diet.
Managed lab, ordering supplies, interacting with collaborating labs, and updating protocols and inventories.
Set up instruments and laboratory equipment used to conduct and monitor experiments.
Physiology, UT Southwestern Medical Center • Dallas, TX• May 2010 to May 2011
Research Technician
Played key role as assistant to post-doctoral researcher preparing genetic experiments using Drosophila Melanogaster (fruit fly) to study synaptic function and vesicular trafficking. Work published in Journal of Biological Chemistry, 2012 (The synaptic vesicle SNARE neuronal synaptobrevin promotes endolysosomal degradation and prevents neurodegeneration).
Key Accomplishments:
Carried out required experimental procedures, including Western Blotting, SDS-PAGE and immunohistochemistry.
Prepared materials needed for experiments, including tissues, antibodies, and reagents.
University of Texas at Dallas • Dallas, TX • August 2009 to May 2010
Teaching Assistant
Facilitated instruction in basic concepts and procedures for lab experiments.
Key Accomplishments:
Prepared materials and equipment needed for experiments, including reagents, solutions, bacterial plates, cell cultures, PCR and blots; sterilized equipment.
Designed assessment tools, evaluating students based on performance and examinations and providing effective feedback aligned with course objectives and intended outcomes.
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