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State University Engineering

Location:
Phoenix, Arizona, United States
Salary:
40/hr
Posted:
August 12, 2018

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Resume:

RESUME

THOMAS SEBASTIAN MS, ASCP (MB)

Mayo Clinic

**** * **** **** ***** ac6m0o@r.postjobfree.com

Phoenix, AZ-85054 Cell Phone –601-***-****

EDUCATION

MSc (Biology, specialization in Biotechnology), 1995

Bachelor of Science, 1993

CERTIFICATION

Certified Molecular Biologist ( MB) –Certified on 10-30-2015

PROFESSIONAL EXPERIENCE

1. Clinical Lab Scientist, Mayo Clinic, Phoenix, AZ.

2. MLS Instructor (Molecular Diagnosis/Techniques) Brookline College Since Jan 2016 to present

3. Molecular Biologist in Department of Neuroscience/Biodesign Institute, Department of Infectious disease and vaccinology, Arizona State University Mar 2015-Aug 2015

Role of miRNA in drug seeking behavior in animal models.

4. Molecular Biologist (Scientist, Faculty), University of Mississippi Medical

Center, Jackson, MS from Sep 2009 to Feb 2012

Experience in gene expression by microarray and its validation through

RTPCR in human microvascular endothelial cells.

Experience with techniques such as genotyping, cloning, microarray, miRNA, RTPCR etc.

Has successfully initiated and executed multiple research projects in genetics and molecular biology/bioinformatics (system biology).Successfully coordinated projects, supervised and trained scientific personnel and research students. Excellent managerial, supervisory, communication and interactive skills.

Gained experienced in PCR based molecular marker technologies and molecular

biology techniques / protocols such as - genomic DNA extraction, DNA quantification, DNA cloning, Plasmid DNA Isolation, RAPD, RT PCR, miRNA, microarray, blotting (Western), Isozyme analysis DNA genotyping,agarose / polyacrylamide gel electrophoresis, tissue culture.

Experience in genetic Engineering techniques such as plasmid construction, recombinant DNA techniques.

5. Research Scientist. Mississippi State University, Mississippi State from Mar 2007 to Sep 2009

Experience in microarray and biological pathway analysis of the gene expression profile of immune genes that regulate innate immune responses modulated by ethanol.

Gained experience in identifying the effect of immuno-glucocorticcoid hormone in regulation of the immune response by computational methods and molecular biology.

Developed skills in genetic engineering and gene manipulation of ChannelnCatfish Viruse ( CCV) to develop vaccine against CCV viruses, a common fish pathogen in USA and Israel.

Trained in microarray using GeneTraffic and signaling pathway analysis tools through Ingenuity pathway analysis and pathway studio at Life Science and Biotechnology Institute (LSBI) Mississippi State University

6. Scientist-Indiana University Purdue University, Indianapolis from

Oct 2005 to Mar 2007

POSTER PRESENTATION

Molecular Mechanism of the inhibition of the innate immune response to LPS by sodium methyldithiocarbonate. X.Deng; T.Sebastian;W.Tan;S.B.Pruett. 49th Annual Meeting Society of Toxicology, Salt Lake City, Utah. March 7 – 11, (2010): Immunotoxicology session

Xiaomin Deng, Sebastian T, Wei Tan and Stephen B. Pruett (2010).Molecular mechanism of the inhibition of the innate immune response to LPS by sodium methyldithiocarbamate. Abstract accepted for presentation for 49th SOT annual meeting 2009, Salt Lake City, Utah.

Sebastian T, Glover M, Pruett SB, (2009). A genomic approach to an innate immuno-regulatory mechanism modulated by ethanol : Microarray and Pathway analysis. Abstract accepted for presentation for 48th SOT annual meeting 2009, Baltimore, Maryland.

Tan,W, Crittenden, P;Liu, Don, Sebastian, T,Pruett, SB (2009). Sodium Methyldithiocarbamate as a probe for mechanism of lethality in sepsis. Abstract accepted for presentation for 48th SOT annual meeting 2009, Baltimore, Maryland.

Pruett, SB; Tan, W; Liu, Don; Sebastian, T (2008). Immunotoxicity and the innate Immune System Presented at Japan (2008).

M.Palakal, Sebastian,T and Shielly Hartanto (2006). Biomedical data mining and integration: A comparative analysis of cells in Inflammation, EAE and MS at Third Annual Indiana Bioinformatics Conference-IUPUI Campus, May-2006.

MEMBERSHIPS IN PROFESSIONAL ORGANISATIONS

Genetic Society of America

American Heart Association

ACTIVITIES

Volunteered in cancer research in VA hospital, Indianapolis, IN (2005). hNMNAT, IMPDH activity of in colon cancer cells and its metabolism to Tiazofurin drug.

REFERENCE

Reference can be provided upon request.



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