Curriculum Vitae

Dr. Yeshwant Vijay Kurhe

Postdoctoral Research Fellow

Microbial Pathogens & Immunity

Rush University Medical Center

Chicago, IL-60612, United States

Email: ac6jw2@r.postjobfree.com

ac6jw2@r.postjobfree.com

Mobile: +1-312-***-****

RESEARCH AREA

Neuroscience; Alzheimer’s Disease, Down Syndrome, Depression, Anxiety, Parkinson’s Disease

Neuropsychiatric Disorders Associated with Metabolic Disorders

Drug Addiction, NeuroAIDS

PROFESSIONAL EXPERIENCE

May 2018 – till date

Postdoctoral Research Fellow at Rush University Medical Center, Chicago, IL, US

Project: Role of calcium firing in the compromised neurocognitive behavior and functions in HIV infected cocaine abusers. I am mainly studying the excitability of medial prefrontal cortex pyramidal neurons in HIV transgenic rat model exposed to cocaine self administration.

May 2017- May 2018

Postdoctoral Research Associate at University of Illinois at Chicago, IL, US

Project: The signaling pathways involved in cognitive failure in Alzheimer’s disease

(AD). The project was focused to study the compromised neurogenesis in AD. It involved standardization and of development of MEK5; Nestin Cre transgenic animal model of neurogenesis.

Feb 2012 – May 2017

Ph.D at BITS Pilani, Pilani Campus, Rajasthan, India (Thesis defended: 5th Jan 2018).

Studied the biological mechanisms linking depression and obesity, and 5-HT3 receptor antagonists as a novel therapeutic approach to control the raising global burden of depression co-morbid with obesity.

As a part of teaching practice, courses handled for first degree (B. Pharm) and higher degree (M. Pharm) are Advanced Pharmacology, Pharmacological Screening Methods, Anatomy Physiology and Hygiene.

Aug 2010 - Feb 2012 (Post- M. Pharmacy)

Research Executive at Orchid Research Laboratories Limited, Chennai, India

Work areas: dermatitis, psoriasis, IBD, asthma and COPD

Responsibilities: planning and performing various in vitro and in vivo studies in rodents in these disease areas, standardization of animal models and screening NCEs in various animal models.

June 2009 to Aug 2010 (Post- M. Pharmacy)

Lecturer at RMP’s Bhalchandra College of Pharmacy, Pune, India

Advanced Pharmacology: Theory and laboratory experiments

Anatomy Physiology and Hygiene: Theory and laboratory experiments ACADEMIC CREDENTIALS

Ph.D in Pharmacology from Birla Institute of Technology & Science (BITS) Pilani, Pilani Campus, Rajasthan, India (2012-2017, Thesis defended on 5th Jan 2018).

M. Pharmacy in Pharmacology from KLE’S College of Pharmacy, Hubli, India (2007- 2009) (76.50 %).

B. Pharmacy from SVPM’S College of Pharmacy, Malegaon (BK), Baramati, Pune, India (2002-2006) (61.42%).

Ph. D SUMMARY (Feb 2012 to May 2017)

Birla Institute of Technology &Science (BITS) Pilani, Pilani Campus Ph.D research pursued at BITS Pilani, Pilani campus, Rajasthan under the guidance of Prof. R. Mahesh, Professor of Pharmacy, Dean Faculty Affairs at BITS Pilani, India. Title: Behavioral and Neuro-pharmacological Screening of Potential Serotonergic Modulators for Depression Co-morbid with Obesity

Summary: The antidepressant-like effect of 5-HT3 receptor antagonists through allosteric modulation of serotonergic system is well evident from several research investigations. 5- HT3 receptor antagonists, ondansetron, QCM-4 and 4a modulated serotonergic transmission and hence, are involved in regulation of HPA-axis hyperactivity by increasing the cAMP and BDNF concentrations in the hippocampus, improved leptin and insulin sensitivity, regulate blood glucose, and reversed oxidative stress associated with depressive behavior in HFD fed animals. Improved cAMP and BDNF levels in the hippocampus by serotonin leads to possibility of neuronal survival that may be crucial in the treatment of depression associated with obesity.

M. PHARM DISSERTATION SUMMARY

M. Pharm (Pharmacology) dissertation work carried out at KLE’S College of Pharmacy, Hubli, India Under the guidance of Prof. B.C. Koti, Head, Department of Pharmacology.

Title: Evaluation of Neuro-pharmacological Effects of Some Novel Benzopyran- 2-one Derivatives in Swiss albino Mice

TECHNICAL PROFICIENCY

In-vivo (Behavioral assays and Techniques)

Expertise in handling and maintaining colonies of various mouse models of Alzheimer’s disease; APPswe, MEK5, 5XFAD, and others Balbc, BL6/C57 and Fischer 344 transgenic rat model.

Small animal surgery; craniotomy (stereotaxic injection).

Models for learning and memory such as Morris water maze (MWM), fear conditioning, novel object reorganization (NOR) and pattern separation.

Chronic models of depression such as; Chronic unpredictable mild stress (CUMS), exogenous corticosterone (CORT), olfactory balbectomy (OBX) induced depression.

Screening of HFD fed obese animals for behavioral changes using models of depression and anxiety.

Hands-on experience in dosing rodents (mice and rats) by various routes viz., Oral, i.m., i.p., i.v., s.c. and collection of aqueous humor and blood.

Animal models of inflammatory bowel disease (TNBS/DSS induced colitis), uveitis

(LPS induced uveitis) and dermatitis (oxazolone/TPA/DNFB/TDI induced dermatitis).

Electrophysiology: Whole cell patch clamp

Molecular biology assays

Immunohistochemistry (IHC) Genotyping

Immunocytochemistry (ICC) DNA isolation

Western Blotting RNA isolation,

RT-PCR Gel electrophoresis

In-vitro-(Biochemical assays)

Biochemical estimation including; glucose, triglycerides, cholesterol, malonaldehyde (MDA) and glutathione (GSH)

ELISA; Insulin, leptin, IL-6 and TNF-α, corticosteroids, BDNF, cAMP, serotonin

Histological studies of mouse brain hippocampus

Stereology & Confocal imaging.

Cell Culture Techniques (Basic training)

Neurosphere culture

Induced pluripotent stem cell (iPSC)

RESEARCH PUBLICATIONS FROM THESIS WORK

1. Kurhe Y, Mahesh R, Devadoss T (2017). Novel 5-HT3 receptor antagonist QCM-4 attenuates depressive-like phenotype associated with obesity in high fat diet fed mice. Psychopharmacology 234:1165-1179.

2. Kurhe Y, Mahesh R (2015). Ondansetron attenuates co-morbid depression and anxiety associated with obesity by inhibiting the biochemical alterations and improving serotonergic neurotransmission. Pharmacol Biochem Behav 136:107- 116.

3. Kurhe Y, Mahesh R, Thangaraj D (2015). QCM-4, a 5-HT3 receptor antagonist ameliorates plasma HPA axis hyperactivity, leptin resistance and brain oxidative stress in depression and anxiety-like behavior in obese mice. Biochem Biophys Res Commun 456: 74-79.

4. Kurhe Y, Mahesh R, Gupta D, Devadoss T (2014). QCM-4, a serotonergic type 3 receptor modulator attenuates depression co-morbid with obesity in mice: An approach based on behavioral and biochemical investigations. Eur J Pharmacol 740: 611-618.

5. Kurhe Y, Mahesh R, Gupta D (2014). Ondansetron attenuates depression co-morbid with obesity in obese mice subjected to chronic unpredictable mild stress; an approach using behavioral battery tests. Metab Brain Dis 29: 701-710. 6. Kurhe Y, Radhakrishnan M, Gupta D, Thangaraj D (2014). QCM-4 a novel 5-HT3 antagonist attenuates the behavioral and biochemical alterations on chronic unpredictable mild stress model of depression in Swiss albino mice. J Pharm Pharmacol 66: 122-132.

7. Kurhe Y, Mahesh R (2017). Ondansetron ameliorates depression associated with obesity in high fat diet fed experimental mice: an investigation based on the behavioral, biochemical and molecular approach. Indian J Pharmacol 49: 290-296. 8. Kurhe Y, Mahesh R (2015). Mechanisms linking depression co-morbid with obesity: An approach for serotonergic type 3 receptor antagonist as novel therapeutic intervention. Asian J Psychiatr 17: 3-9.

RESEARCH PUBLICATIONS OTHER THAN THESIS WORK

1. Kurhe Y, Mahesh R (2016). Pioglitazone, a PPAR-Υ agonist rescues depression associated with obesity using chronic unpredictable mild stress model in experimental mice. Neurobiology of Stress 3: 114-121. 2. Kurhe Y, Mahesh R, Gupta D (2014). Effect of a Selective Cyclooxygenase Type 2 Inhibitor Celecoxib on Depression Associated with Obesity in Mice: An Approach Using Behavioral Tests. Neurochem Res 39: 1395-1402. 3. Gupta D, Kurhe Y, Radhakrishnan M (2014). Antidepressant effects of insulin in streptozotocin induced diabetic mice: Modulation of brain serotonin system. Physiol Behav 129C:73-78.

4. Kulkarni NM, Jaji MS, Shetty P, Kurhe YV, et al (2014). A novel animal model of metabolic syndrome with non-alcoholic fatty liver disease and skin inflammation. Pharm Biol 53, 1110-1117.

RESEARCH SYMPOSIUMS & AWARDS

1. Kurhe Y, Mahesh R, Thangaraj D. Mechanisms linking depression associated with obesity through serotonergic modulation in experimental mice. SfN 46th Annual Meeting, Neuroscience 2016, San Diego, CA, United States, Nov 12-16, 2016.

(Received grants from SfN-IBRO).

2. Kurhe Y, Mahesh R, Gupta D, Devadoss T. Antidepressive-like effect of a novel 5- HT3 receptor antagonist (4a) against depression co-morbid with obesity in mice subjected to chronic stress. 9th FENS Forum of Neuroscience, Milan, Italy, July 5- 9, 2014 (Received grants from ICMR).

3. Kurhe Y, Mahesh R, Devadoss T. Serotonin type 3 receptor antagonist (4- phenylpiperazin-1-yl) (quinoxalin-2-yl) methanone (4a) attenuates depression co- morbid with obesity by promoting serotonergic neuromodulation and reversing insulin resistance in experimental mice. IBRO/APRC Varanasi School (Advances in Neurobiology), Varanasi, India, Sept-5-20, 2015 (Received grants from IBRO). COMPUTER PROFICIENCY

Ability to use a variety of work related software such as MS office, statistical software, Graph Pad Prism, Efficient in scientific information retrieval using search engines in internet (PubMed, Science direct, Highwire press, etc.). PERSONAL PROFILE

Date of Birth: 24th Jan 1984

Sex: Male

Marital Status: Married

Languages known: English, Hindi, Marathi

Permanent Address: At/Post: Kharwandi, Tal: Newasa, Dist: Ahmednagar, Pin: 414602,

Maharashtra, India.

DECLARATION

I hereby declare that the above information is correct known to the best of my knowledge. Thanking You,

Dr. Yeshwant Vijay Kurhe Place: Chicago

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