Maria De Los Angeles Mercado

**** * ******* ***. ***. 410

Little Rock, AR 72205.

Phone: 501-***-****

Email: ac6j97@r.postjobfree.com

Professional Experience

Sr. Scientist

SCA Pharmaceuticals February 2014 to Present

Little Rock, AR 72205

• Developed the Environmental Monitoring Program for a new pharmaceutical startup.

• Executed PQ for Biomerieux Chemunex Scan RDI sterility method.

• Performed IQ/OQ for Sampl’Air AES and Endotoxin equipment.

• Performs Sterility testing on finished products using Scan RDI and Steritest membrane filtration.

• Performs Endotoxin testing, gram staining and environmental monitoring.

• Act as a mentor and trainer to all microbiology personnel.

• Initiate Non-Conformance reports, Out of Limits (OOL) and Out of Specifications (OOS) investigation.

• Represent the manager in meetings when is necessary.

• Member of the Health and Safety Committee Team.

Research Assistant

University of Arkansas for Medical Sciences October 2005 - January 2014

Little Rock, AR 72205

• Performed Phase I Clinical Trial testing for Human Papillomavirus Virus (HPV) vaccine.

• Worked with human subject for regulatory T Cells staining, Human leukocyte antigen (HLA) typing on DNA, Peripheral blood mononuclear cell (PBMC) isolation, CD14 selection, HPV genotyping.

• Received training in T-cell (Thymocytes cells) cloning, CD3 T-cell line and Enzyme-Linked ImmunoSpot (Elispot).

• Prepared Standard Operation Procedures (SOP) for HLA typing.

• Lysed cells for protein assays and western blot techniques.

• Prepared DNA and DNA plasmid for Polymerase Chain Reaction (PCR) and Real Time PCR.

• Participated and contributed to Scholarly Journal meetings.

• Managed the organization of the laboratory and ordered supplies.

• Participated in meetings with principal investigators for the development of new projects at Veteran's Hospital and University of Arkansas for Medical Sciences.

Laboratory Assistant

Saline Memorial Hospital August 2011 - January 2014

Benton, AR 72015 United States

• Processed the specimens ordered by medical professionals.

• Evaluated blood, stool and urine samples using different kits.

• Received supplies orders and prepared specimens to send out for testing.

• Served as Interpreter for non-English speaking Hispanic patients.

Research Technician III

University of Lincoln Nebraska, November 2002 - July 2004

Lincoln, Nebraska

• Identified bacterial and fungal Pathogens in the Bacteriology laboratory.

• Performed serological test by ELISA, AGID test, Latex Agglutination, Serum Neutralization.

• Prepared tissues for Virus Isolation.

• Performed validation and certification of manufactured test kit for Chronic Wasting Disease.

• Served as Spanish-English interpreter to international visitors and researchers.

Laboratory Technician III

Abbott Pharmaceutical Inc. July 1981 - May 1992 Chemical Division Barceloneta, P.R., US

• Supervised and directed the daily work in the laboratory, included conducting chemical analyses of Raw Materials to produce finishing products and final evaluation of finished products.

• Attended to meetings with the Planning department team, to designate the priorities for Raw Materials and Finished product release.

• Cross trained in Finishing products, Raw materials and Waste Water laboratories.

Provided guidelines and directions to the laboratory technicians. Operated and maintained laboratory equipment.

• Provided seminars on Safety training to the facility personnel.

• Assisted on internal audits in preparation for FDA, OSHA, and EPA inspections.

Education

• Bachelor’s Degree in Biology, Minor in Chemistry

Inter-American University of Puerto Rico

Arecibo, P. R. US

Membership

• ASQ.Org

Laboratory Instrumentation and Operation

• Automated High-Performance Liquid Chromatography (HPLC).

• Automated Gas Chromatography (GC).

• Atomic Absorption Flame Photometer.

• Dissolution Apparatus (Method I, II, Dissolution Rollers).

• Karl Fisher.

• UV-Visible Spectrophotometer.

• Fluorescent, Compound, Inverted and Dark Field Microscope.

• Bio-tek Ceres 900 Plate Reader.

• Electrophoresis Apparatus.

• Bio-Rad Quantity One 4.6 (Chemi Doc XRS).

• Polymerase Chain Reaction Work Station.

• Gene Amp Polymerase Chain Reaction System 2400 and 9600.

• Gene Quant RNA/DNA Calculator.

• Applied Biosystem Instrument Fast 7500, 7900HT.

• Beckman Coulter, Vi-Cell.

• Endosafe Portable and Multi Test System Kinetic Reader.

• AES Sampl’Air Lite.

• Steritest Equinox Pump for Laminar Flow Hood.

• Biomerieux ChemScan RDI.

• Nikon Eclipse 50i Chemunex.

• Air Borne Particle Counter MET One HHPC 3+ for Non-Viable Air test.

• Nikon Digital Sight DS-F12 Eclipse Ci.

Professional References

• Christ Knecht 281-***-****.

• Jacob Wansley 919-***-****.

• Mayumi Nakagawa, MD, PhD 501-***-****.

• Jeremy Rotton 501-***-****.

Personal Reference

• Father Mark Wood 501-***-****.

• Hector Torres 787-***-****.

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