Medical Assistant
Resume:
Maria De Los Angeles Mercado
Little Rock, AR 72205.
Phone: 501-***-****
Email: *******@*********.***
Professional Experience
Sr. Scientist
SCA Pharmaceuticals February 2014 to Present
Little Rock, AR 72205
• Developed the Environmental Monitoring Program for a new pharmaceutical startup.
• Executed PQ for Biomerieux Chemunex Scan RDI sterility method.
• Performed IQ/OQ for Sampl’Air AES and Endotoxin equipment.
• Performs Sterility testing on finished products using Scan RDI and Steritest membrane filtration.
• Performs Endotoxin testing, gram staining and environmental monitoring.
• Act as a mentor and trainer to all microbiology personnel.
• Initiate Non-Conformance reports, Out of Limits (OOL) and Out of Specifications (OOS) investigation.
• Represent the manager in meetings when is necessary.
• Member of the Health and Safety Committee Team.
Research Assistant
University of Arkansas for Medical Sciences October 2005 - January 2014
Little Rock, AR 72205
• Performed Phase I Clinical Trial testing for Human Papillomavirus Virus (HPV) vaccine.
• Worked with human subject for regulatory T Cells staining, Human leukocyte antigen (HLA) typing on DNA, Peripheral blood mononuclear cell (PBMC) isolation, CD14 selection, HPV genotyping.
• Received training in T-cell (Thymocytes cells) cloning, CD3 T-cell line and Enzyme-Linked ImmunoSpot (Elispot).
• Prepared Standard Operation Procedures (SOP) for HLA typing.
• Lysed cells for protein assays and western blot techniques.
• Prepared DNA and DNA plasmid for Polymerase Chain Reaction (PCR) and Real Time PCR.
• Participated and contributed to Scholarly Journal meetings.
• Managed the organization of the laboratory and ordered supplies.
• Participated in meetings with principal investigators for the development of new projects at Veteran's Hospital and University of Arkansas for Medical Sciences.
Laboratory Assistant
Saline Memorial Hospital August 2011 - January 2014
Benton, AR 72015 United States
• Processed the specimens ordered by medical professionals.
• Evaluated blood, stool and urine samples using different kits.
• Received supplies orders and prepared specimens to send out for testing.
• Served as Interpreter for non-English speaking Hispanic patients.
Research Technician III
University of Lincoln Nebraska, November 2002 - July 2004
Lincoln, Nebraska
• Identified bacterial and fungal Pathogens in the Bacteriology laboratory.
• Performed serological test by ELISA, AGID test, Latex Agglutination, Serum Neutralization.
• Prepared tissues for Virus Isolation.
• Performed validation and certification of manufactured test kit for Chronic Wasting Disease.
• Served as Spanish-English interpreter to international visitors and researchers.
Laboratory Technician III
Abbott Pharmaceutical Inc. July 1981 - May 1992 Chemical Division Barceloneta, P.R., US
• Supervised and directed the daily work in the laboratory, included conducting chemical analyses of Raw Materials to produce finishing products and final evaluation of finished products.
• Attended to meetings with the Planning department team, to designate the priorities for Raw Materials and Finished product release.
• Cross trained in Finishing products, Raw materials and Waste Water laboratories.
Provided guidelines and directions to the laboratory technicians. Operated and maintained laboratory equipment.
• Provided seminars on Safety training to the facility personnel.
• Assisted on internal audits in preparation for FDA, OSHA, and EPA inspections.
Education
• Bachelor’s Degree in Biology, Minor in Chemistry
Inter-American University of Puerto Rico
Arecibo, P. R. US
Membership
• ASQ.Org
Laboratory Instrumentation and Operation
• Automated High-Performance Liquid Chromatography (HPLC).
• Automated Gas Chromatography (GC).
• Atomic Absorption Flame Photometer.
• Dissolution Apparatus (Method I, II, Dissolution Rollers).
• Karl Fisher.
• UV-Visible Spectrophotometer.
• Fluorescent, Compound, Inverted and Dark Field Microscope.
• Bio-tek Ceres 900 Plate Reader.
• Electrophoresis Apparatus.
• Bio-Rad Quantity One 4.6 (Chemi Doc XRS).
• Polymerase Chain Reaction Work Station.
• Gene Amp Polymerase Chain Reaction System 2400 and 9600.
• Gene Quant RNA/DNA Calculator.
• Applied Biosystem Instrument Fast 7500, 7900HT.
• Beckman Coulter, Vi-Cell.
• Endosafe Portable and Multi Test System Kinetic Reader.
• AES Sampl’Air Lite.
• Steritest Equinox Pump for Laminar Flow Hood.
• Biomerieux ChemScan RDI.
• Nikon Eclipse 50i Chemunex.
• Air Borne Particle Counter MET One HHPC 3+ for Non-Viable Air test.
• Nikon Digital Sight DS-F12 Eclipse Ci.
Professional References
• Christ Knecht 281-***-****.
• Jacob Wansley 919-***-****.
• Mayumi Nakagawa, MD, PhD 501-***-****.
• Jeremy Rotton 501-***-****.
Personal Reference
• Father Mark Wood 501-***-****.
• Hector Torres 787-***-****.
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