Medical Human

Nishant Nandanwar, Ph.D

***** **** ********* ****,

Dolphin Square LLC,

Unit: 314, Wauwatosa,

Milwaukee, Wisconsin, 53226

*********.*******@*****.***

Mobile No: +1-414-***-**** / +1-414-***-****

Research Experience

Jan 2014 – July 2017 Post Doctoral Fellow, Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad,

(A.P.), India

Projects: A) Characterization of inhibitory potential of selective probiotic bacteria against human gastric pathogen, Helicobacter pylori

B) Population genetic structure and molecular epidemiology of clinical multi-drug resistant (MDR) Escherichia coli strains from geographically distinct human subjects in India Nov 2010 - Oct 2013 Doctorate Student, Institute of Microbiology and Epizootics, Freie University, Berlin, Germany

Ph.D. Thesis Title “Functional genotyping of extraintestinal pathogenic E.coli (ExPEC) belonging to the highly pathogenic sequence type 95 reveals the zoonotic nature of human and avian strains”.

Supervisor: Prof. Dr. Lothar H. Wieler, President, Robert Koch Institute, Berlin, Germany

Jan 2009 - Nov 2010 Project Fellow, Department of Biotechnology, School of Life Sciences, University of Hyderabad, (A.P.), India

Spacer oligotyping (Spoligotyping) in epidemiological studies of M. tuberculosis using clinical isolates of M. tuberculosis

Cloning, expression and purification of hypothetical ORF, HP0431 from 26695 strain of H. pylori identified as serine threonine phosphatase

Sep 2008 - Dec 2008 Project Assistant, Centre for DNA Fingerprinting and Diagnostics

(CDFD), Hyderabad, A.P. India

Molecular epidemiological studies of M. tuberculosis to assess immune escape by novel strains emerging in the catchments of international vaccine trial in Chittor District of Andhra Pradesh, India.

June 2006 - Aug 2006 Project Trainee, (M. Sc Dissertation), National Environmental Engineering Research Institute (NEERI), Nagpur, Maharashtra, India

Biodegradation of Aromatic Compounds- Pyrimidine, Benzene, Toluene and Xylene (PBTX)

Publications (Citations – 249, h-index – 7, i10-index – 7) 1. Hussain A, Shaik S, Ranjan A, Nandanwar N, Tiwari SK, Mohammad M, Baddam R, Qureshi IA, Semmler T, Wieler LH, Islam AM, Chakravortty D, Ahmed N. Risk of Zoonotic Transmission of Antimicrobial Resistant Escherichia coli from Commercial Broiler and Free- Range Retail Chicken in India. 18 Oct 2017. Frontiers in Microbiology. 8:2120. https://www.ncbi.nlm.nih.gov/pubmed/29180984

2. Shaik S, Ranjan A, Tiwari SK, Hussain A, Nandanwar N, Kumar N, Jadhav S, Semmler T, Baddam R, Islam MA, Alam M, Wieler LH, Watanabe H, Ahmed N. Comparative Genomic Analysis of Globally Dominant ST131 Clone with Other Epidemiologically Successful Extraintestinal Pathogenic Escherichia coli (ExPEC) Lineages. 24 Oct 2017. mBio, (American Society for Microbiology Publication) 8(5), pp. e01596-17. https://www.ncbi.nlm.nih.gov/pubmed/29066550

3. Ranjan A, Shaik S, Nandanwar N, Hussain A, Tiwari SK, Semmler T, Jadhav S, Wieler LH, Alam M, Colwell RR, Ahmed N. Comparative Genomics of Escherichia coli Isolated from Skin and Soft Tissue and Other Extraintestinal Infections. 15 Aug 2017. mBio, (American Society for Microbiology Publication) 8(4), pp.e 01070-17. https://www.ncbi.nlm.nih.gov/pubmed/28811343

4. Ranjan A, Shaik S, Mondal A, Nandanwar N, Hussain A, Semmler T, Kumar N, Tiwari SK, Jadhav S, Wieler LH, Ahmed N. Molecular Epidemiology and Genome Dynamics of New Delhi Metallo-beta-Lactamase-Producing Extraintestinal Pathogenic Escherichia coli Strains from India. 21 Oct 2016. Antimicrobial agents and chemotherapy, 60(11), pp. 6795-6805. https://www.ncbi.nlm.nih.gov/pubmed/27600040

5. Ranjan A, Shaik S, Hussain A, Nandanwar N, Semmler T, Jadhav S, Wieler LH, Ahmed N. Genomic and Functional Portrait of a Highly Virulent, CTX-M-15-Producing H30-Rx Subclone of Escherichia coli Sequence Type 131. 18 Sep 2015. Antimicrobial agents and chemotherapy, 59(10), pp. 6087-6095.

https://www.ncbi.nlm.nih.gov/pubmed/26195517

6. Hussain A, Ranjan A, Nandanwar N, Babbar A, Jadhav S, Ahmed N. Genotypic and phenotypic profiles of Escherichia coli isolates belonging to clinical sequence type 131

(ST131), clinical non-ST131, and fecal non-ST131 lineages from India. 22 Sep 2014. Antimicrobial agents and chemotherapy, 58 (12), pp. 7240-7249. https://www.ncbi.nlm.nih.gov/pubmed/25246402

7. Nandanwar N, Janssen T, Kuhl M, Ahmed N, Ewers C, Wieler LH. Extraintestinal pathogenic Escherichia coli (ExPEC) of human and avian origin belonging to sequence type complex 95 (STC95) portray indistinguishable virulence features. 27 Jun 2014. International Journal of Medical Microbiology, 304(7), pp. 835-842. https://www.ncbi.nlm.nih.gov/pubmed/25037925

8. Ewers C, Dematheis F, Singamaneni HD, Nandanwar N, Fruth A, Diehl I, Semmler T, Wieler LH. Correlation between the genomic o454-nlpD region polymorphisms, virulence gene equipment and phylogenetic group of extraintestinal Escherichia coli (ExPEC) enables pathotyping irrespective of host, disease and source of isolation. 16 Sep 2014. Gut pathogens, 6(1), pp. 37.

https://www.ncbi.nlm.nih.gov/pubmed/25349632

9. Hussain A, Ewers C, Nandanwar N, Guenther S, Jadhav S, Wieler LH, Ahmed N. Multiresistant uropathogenic Escherichia coli from a region in India where urinary tract infections are endemic: genotypic and phenotypic characteristics of sequence type 131 isolates of the CTX-M-15 extended-spectrum-beta-lactamase-producing lineage. 08 Oct 2012. Antimicrobial agents and chemotherapy, 56(12), pp. 6358-6365. https://www.ncbi.nlm.nih.gov/pubmed/23045357

10. Avasthi TS, Kumar N, Baddam R, Hussain A, Nandanwar N, Jadhav S, Ahmed N. Genome of multidrug-resistant uropathogenic Escherichia coli strain NA114 from India. 17 Jun 2011. Journal of bacteriology, 193(16), pp. 4272-4273. https://www.ncbi.nlm.nih.gov/pubmed/21685291

11. Ahmed N, Tenguria S, Nandanwar N. Helicobacter pylori--a seasoned pathogen by any other name. 23 Dec 2009. Gut pathogens, 1(1), pp. 24. https://www.ncbi.nlm.nih.gov/pubmed/20030808

12. Nandanwar N, Hussain A, Ranjan A, Jadhav S, Ahmed N. Population structure and molecular epidemiology of human clinical multi-drug resistant (MDR) Escherichia coli strains from Pune, India (Manuscript in preparation). Conference Abstract Published:

1. Nishant Nandanwar, Arif Hussain, Amit Ranjan, Savita Jadhav, Niyaz Ahmed. Population structure and molecular epidemiology of human clinical multi-drug resistant (MDR) Escherichia coli strains from Pune, India. April 2016. International Journal of Infectious Diseases http://www.ijidonline.com/article/S1201-9712(16)30720-2/fulltext Education

2010-2014 Ph. D, (magna cum laude)

Thesis Title “Functional genotyping of extraintestinal pathogenic E. coli

(ExPEC) belonging to the highly pathogenic sequence type 95 reveals the zoonotic nature of human and avian strains”.

Supervisor: Prof. Dr. Lothar H. Wieler, President, Robert Koch Institute, Berlin, Germany

Former Director, Institute of Microbiology and Epizootics, Berlin, Germany 2005-2007 M. Sc (Biotechnology), Saifia College of Science & Education, Bhopal, M.P., India (Degree aggregate – 71%)

2003-2005 B. Sc (Biotechnology), Extol Institute of Management & Life Science, Bhopal, M.P., India (Degree aggregate – 72%)

Technical skills

Received Training in Medical Biotechnology at Department of Research, Bhopal Memorial Hospital & Research Centre, Bhopal, (M.P). India

Laboratory Skills Related to Microbiology

Expertise and knowledge in microbiological procedures including aseptically maintenance of microbial stock culture, sub-culturing of microbes

Isolation of microorganisms, mainly bacteria, from different clinical and environmental samples

Determined antibiotic resistance profiles of multi drug resistant organisms

Performed co-culture experiments with probiotic strains and pathogenic strains collected from different clinical settings

Determined growth of organisms under different stress conditions

Determined biofilm forming capabilities and serum resistance of E. coli isolated from different clinical samples

Laboratory Skills Related to Molecular Biology

Isolation of DNA, RNA and plasmid from different sources

PCR, Real time PCR, Cloning, Spoligotyping, Agarose gel Electrophoresis

Protein expression, purification and SDS PAGE, Western Blotting, ELISA

Pulse field gel electrophoresis, multilocus sequence typing Laboratory Skills Related to Cell Culture

Working experience with Human embryonic kidney cells (HEK293-T), ACHN, T24, MDCK1, AGS, CaCo-2 cell line and human macrophages

Bacterial infection experiments such as adhesion and invasion assays in cell lines and in Zebrafish and intracellular survival of pathogenic organisms. Skills Related to Bioinformatics

Basic Bioinformatics, phylogenetic analysis using MEGA and SPSS software, identification of SNPs

Teaching experience

Practical classes for M. Sc. Biotechnology students

Lectures (3 and 2 credits courses for 2 semesters) for Integrated B. Tech M. Tech, Integrated M. Sc. Ph. D and M. Sc students

Guided three M. Sc students for Master thesis, and guided Ph. D students to design infection experiments

Memberships

Life time Member of International Society of Infectious Diseases

Life time Member of the National Research Platform for Zoonoses, Berlin, Germany Language Proficiency

B2 Level of German language

Proficient in MS Office

Writing and reviewing of manuscripts

Awards and Fellowships

01/01/2016 National Post-Doctoral Fellowship Grant funded by Science and Engineering Research Board (SERB), Dept. of Science and Technology, Government of India 05/03/2016 Best poster award in the category of Medical Microbiology at the 17th International Conference on Infectious Disease (ICID-2016) organized by International society on Infectious Disease (ISID) 03/03/2016 Travel Grant award for Young Investigator from India at the 17th International Conference on Infectious Disease (ICID-2016) organized by International society on Infectious Disease (ISID)

01/01/2014 Dr. D. S. Kothari Post-Doctoral Fellowship Grant funded by University Grants Commission, India

15/07/2013 Won third prize for poster presentation at the 8th “DRS Symposium and presentation seminar – Biomedical Sciences” Department of Veterinary Medicine, Freie University, Berlin, 15th July 2013.

Conferences and Posters

Poster presentation at the 17th International Conference on Infectious Disease (ICID-2016) organized by International society on Infectious Disease (ISID)

Presented poster at the 56th Annual conference of Association of Microbiologist and International Symposium on Emerging Discoveries in Microbiology organized by School of life sciences, Jawaharlal Nehru University, New Delhi, India from 7-10 December 2015.

Attended International Conference on Host-Pathogen Interactions organized by National Institute of Animal Biotechnology, Hyderabad from July 12 – 15, 2014.

Presented poster at the National Symposium on Zoonoses Research, held in Berlin, September 19th-20th 2013.

Presented poster at the 8th DRS Symposium and presentation seminar – Biomedical Sciences Department of Veterinary Medicine, Freie University, Berlin, 15th July 2013.

Presented poster at 7th DRS Symposium and presentation seminar – Biomedical Sciences Department of Veterinary Medicine, Freie University, Berlin, 13th July 2012.

Participated and presented poster in ZIBI Summer Symposium 2012, Berlin Germany on

Global Challenges of Chronic Tropical Infections held during 18-19thJune 2012.

Presentation at the Mini Symposium conducted by DRS – Biomedical Sciences Department of Veterinary Medicine, Freie University, Berlin, 27th February 2012.

Attended XXXIII All India Cell Biology Conference and International Workshop on Cell Cycle Regulation held during December 10-13, 2009. University of Hyderabad, Hyderabad.

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