Ali Zendedel Haghighi
Home Address: **** ********* *****
San Jose, CA 95120
Phone: 510-***-**** (cell)
Cell Culture Lab Manager
Harker High School
San Jose, Ca
Instructor for Chemistry and Biology
National Hispanic University
San Jose, Ca
Instructor for Chemistry and Biology
Consulting Scientist on the conductive polymers’ projects
Consulting Scientist on the Wound healing products
American Century University
San Jose, CA
Setting up the Biotechnology program, as well as the instructor for the program
Scientist for Biofuel projects
Menlo Park, CA
Scientist for Bioassay development
Mountain View, CA
San Francisco, CA
Editor for Biotech courses
San Francisco State University
San Francisco, CA
Instructor for the Clinical Trial program
March 2006- May 2006
Los Angles, CA
Consultant for skin rejuvenation projects
NovaCal Pharmaceuticals, Inc.
Science and Clinical Instructor
San Jose, CA
Consultant for Bioinformatic Projects
San Jose, CA
Consultant for the projects on the skin rejuvenation
Sunrise Technologies, Inc.
Senior Scientist and Clinical Coordinator
MAST Immunosystems, Inc.
Mountain View, CA
Centaur Pharmaceuticals, Inc.
The Cleveland Clinic Foundation
Ph.D. in Biochemistry
Cleveland State University, 1991
M.S. in Biochemistry
Kent State University, 1986
B.A. in Chemistry
Kent State University, 1982
Certification: American Heart Association, BLS Instructor
Teaching Certification for the Surgical Technology
Teaching Certification for the Medical Assistant
Manager of the cell culture lab, testing the effects of anti-cancer drugs on various cancer cell line (Translational Bioscience)
Instructor for the High school Chemistry classes (Harker High School)
Instructor for the first year college chemistry and biology courses (National Hispanic University)
Initiating and supervising the projects on the wound healing products, based on the silver-nano particles’ anti-septic properties (Chitotech Corporation)
Setting up the Biotechnology program and functioning as the main instructor for the program (American Century University)
Working on the projects related to Biomass to biofuel ( Applied Chemicals)
Projects related to the use of nanoparticles as catalyst (LamdaGen Corporation)
Assay development on biosensors, for the detection of biomolecules as well as drugs (LamdaGen Corporation)
Development of an ELISA-based diagnostic assay system, using CavilinkTM polymers, as well as their application in drug delivery systems (Polygenetics, Inc.).
Development of in vitro models and studies to test the effect of the Novacal’s products on wound healing, enhancement of collagen synthesis, as well as their ability to effectively destroy biofilm, generated by infecting bacteria. Also, studies on the selection of possible drug vehicle for the Novacal’s products. (Novacal Pharmaceuticals)
Instructor for the basic sciences (i.e., Anatomy, Physiology, Pharmacology and Microbiology) in a Surgical Technology training program. Also, clinical site instructor for the students receiving training at the area hospitals. (Bryman College).
Study on the effect of Proteases and Reactive Oxygen Species (ROS) on the lens proteins and subsequent Cataract formation. (UCSF)
Study on the role of oxidative damage and UV light in the development of Cataract and Presbyopia and, development of possible methods, to inhibit as well as reverse Cataract and Presbyopia. (Sunrise Technologies)
Study on the mechanism of heat transfer in the cornea after laser treatment and development of a method to treat Myopia, using laser thermal keratoplasty (LTK). Development of biological models to study the long-term effect of laser treatment on the cornea and, development of a biochemical procedure to reduce or inhibit the regression associated with the laser refractive surgery. Designing, implementing and coordinating clinical studies on the use of laser for the treatment of Myopia. Also, designing clinical studies on the biochemical treatment of the laser-treated hyperopia
individuals. (Sunrise Technologies)
Development of a novel and simple procedure for the detection of immune complexes in serum, formed as the result of excessive food allergens. (MAST Immunosystems)
Purification and characterization of a new allergenic protein in Mite
farinae, using different protein purification methods such as: gel filtration chromatography, SDS-PAGE (both analytical as well as preparative), electroelution of protein molecules, Western blotting, ELISA and different protein quantitation methods. (MAST Immunosystems)
Purification and characterization of proteins causing allergic reactions and coupling these proteins to a solid matrix to be used in an allergy-testing device. Also, development of assays (chemical, biochemical and ELISA.) and writing protocols, SOPs, MBRs, QAP and QTS documents for these assays and protein purification procedures to be used by the QA and manufacturing departments. (MAST Immunosystems)
Study on the inhibitory effect of beta-amyloid peptide and methionine sulfoxide on glutamine synthetase and creatine phosphokinase, by the use of enzyme assays and free radical scavengers. (Centaur Pharmaceuticals)
Setting up the Biochemistry laboratory in a startup company. Evaluating and developing biochemical assays, and procedures such as: assays for glutamine synthetase, creatine phosphokinase and lipoxygenase, protein and lipid oxidation assays and brain tissue preparation protocols and writing SOPs to screen compounds as therapeutic agents for neurodegenerative diseases. (Centaur Pharmaceuticals)
Purification and characterization of a human immunosuppressive lymphokine produced and released by a human thymus hybridoma, utilizing the techniques of cell culturing, antibody isolation and preparation, immunoaffinity column preparation, enzyme immunoassay (EIA), antigen purification and preparation, SDS-PAGE (both analytical and preparative), western blotting, bioassays for lymphokine activity measurements, protein
purification and PCR. ( The Cleveland Clinic Foundation)
Study of the mechanism of the low density lipoprotein oxidation by the activated monocytes as well as the oxidation of pure lipids mediated by the free radicals, utilizing the techniques of cell culturing, TLC, lipid isolation and purification, lipid assays, assays for the detection and measurements of oxidized lipids and assays for the detection and measurements of different free radicals. (The Cleveland Clinic Foundation)
Study of enzymes released from PMNs due to the external stimulation, using the enzyme activity assays for various enzymes, protein assay,
SDS-PAGE, the Superoxide Dismutase (SOD) enzyme kinetic studies, and development of a methodology for SOD activity measurements.
Study of the effect of Phenobarbital treatment on the serum lipoprotein, Thromboxane A2 and Prostacyclin, using radioisotope labeling, PAGE and SDS-PAGE, protein purification as well as protein and cholesterol assays.
Lipoprotein classification and their molecular weight distribution, by utilizing the gradient PAGE and analytical ultracentrifugation.
Development of methodology for purification of Herpes Simplex virus by use of skin Fibroblast cell culture, density gradient ultracentrifugation, protein assay and SDS-PAGE.
Study of the changes in liver plasma membrane composition in the cholesterol-fed rats, using the preparative density gradient ultracentrifugation and phosphate and protein assays.
Study of a drug and oligonucleotide interaction, using virus infected skin fibroblast cells and interferons.
Study of the methyl mercury toxicity in Bluegills using the protein and cholesterol assays and SDS-PAGE methods.
1) Ali Zendedel Haghighi, LamDagen’s meta-catalytic surfaces, first case study: Role of the surfaces in the catalytic decomposition of hydrogen peroxide, (2009), White paper, written as a part of the work in LamdaGen coprporation.
2) Ali Zendedel Haghighi, James R. Benson and Nai-Hong Li, Use of highly porous polymer in a diagnostic assay, (2007), American Laboratory, 39 (14), 35-37.
3) A. Z. Haghighi and Robert Wei, Measurement of superoxide dismutase in erythrocytes and whole blood using iodonitrotetrazolium violet, (1998), Anal. Lett., 31 (6), 45.
4) Ali Zendedel Haghighi and Kirk R. Maples, On the Mechanism of the Inhibition of Glutamine Synthetase and Creatine Phosphokinase by Methionine Sulfoxide,(1996), Journal of Neuroscience Research, 43, 107-111.
5) Ali Zendedel Haghighi and Martha K. Cathcart, Subtractive antibody to a human immunosuppressive lymphokine affinity isolates a suppressive factor and blocks its function, (1992), Immunopharmacology, 24, 65-76.
6) Ali Zendedel Haghighi, Study on the release of enzymes from the human Polymorphonuclear Leukocytes and development of Superoxide Dismutase assay by p-Iodonitrotetrazolium Violet, (1991), Ph.D. dissertation, Cleveland State University.
7) Thomas I. Pynadath and Ali Zendedel Haghighi, Inhibition of Thromboxane A2 synthesis in rats treated with Phenobarbital, (1987), Prostaglandins Leukotrienes and Medicine, 28, 61-71.
8) Ali Zendedel Haghighi and Thomas I. Pynadath, Stimulation of
Prostacyclin synthesis in rats treated with Phenobarbital, (1987), Prostaglandins Leukotrienes and Medicine, 28, 267-275.
9) Ali Zendedel Haghighi, Effect of Phenobarbital on low and high density lipoproteins, Thromboxane A2 and Prostacyclin, (1986), M.S. thesis, Kent State University.
10) Hiran M. Data and Ali Zendedel Haghighi, Methylmercuric chloride and serum cholesterol level in Bluegill (Lepomus Macrochirus), (1986), Bull. Environ. Contam. Toxicol., 36, 181-185.
11) Hiran M. Data, S.B. Lall and Ali Zendedel Haghighi, Methyl mercury induced changes in the serum proteins of Bluegills-Lepomus Macrochirus (Teleostel), (1983), Ohio J. Sci., 83, 119-122.
1) Effect of Neutrophil immune response products, proteases and reactive oxygen species, on lens proteins. B.C. Bruno, A. Zendedel Haghighi, T.P. Margolis, S.D. McLeod (2002). The Association for Research in Vision and Opthalmology, 2002 Annual Meeting.
2) Effect of UV light and oxidative damage on the formation of Cataract. A. Zendedel Haghighi, B.W. Woodward (2001), The Association for Research in Vision and Opthalmology, 2001 Annual Meeting, p. S454.
3)On the mechanism of redox reactions in cornea, in the presence or absence of laser treatment. A. Zendedel Haghighi, B.W. Woodward, (2000), The Association for Research in Vision and Ophthalmology, 2000 Annual Meeting, p.S466.
4)A novel and simple procedure for the detection of circulating immune
complexes, formed due to excess food allergens in serum. N.T. Eldredge, V. Marinkovich, A. Zendedel Haghighi, (1999), The XVIIIth Conggress of the European Academy of Allergology and Clinical Immunology, P411.
5)Presence of a 97 kDa allergen in Mite, Dermatophagoides farinae. E.
Fashandi, N. Eldredge, C. Brown and A. Zendedel Haghighi, (1997), International Association of Allergology and Clinical Immunology, ICACA XVI, Supplement No. 4, p. 140.
6) The use of Capillary Electrophoresis for Allergen Extract Standardization, Zychlinsky E., Innerst D., Millward V., Zendedel Haghighi A., Eldredge N., Brown C., (1995), The Second Asian Pacific Congress of Allergology and Clinical Immunology, p. 3.
7) Preparation of a subtractive antibody to hybridoma suppressor factor. A. Zendedel-Haghighi and M. K. Cathcart, (1991), The society for Leukocyte Biology/ Leukocyte Culture Conference joint meeting, supplement 2, p. 103.
8) On the determination of Superoxide Dismutase by Iodonitrotetrazolium Violet. A. Z. Haghighi and R. Wei, (1990), American Society for Biochemistry and Molecular Biology/ The American Association of Immunologists joint meeting, Vol. 4, p. A2304.
9) Release of proteins from the human PMNs treated with PMA or
cyclochalasin B/ FMLP. A. Z. Haghighi and R. Wei, (1989), Federation of American Societies for Experimental Biology, Vol. 3, p. A322.
10) Characteristics of intermediate density lipoproteins. Jan J. Opplt and Ali Z. Haghighi, (1987), American Association for Clinical Chemists, Vol. 33, p. 896.
11)Phenobarbital decreases Thromboxane synthesis and increases
Prostacyclin synthesis in rats. Thomas I. Pynadath and Ali Z. Haghighi, (1985), Federation of American Societies for Experimental Biology, Vol. 44, p. 489.
12) Effect of Phenobarbital treatment on serum lipoproteins in rats. Thomas I. Pynadath and Ali Z. Haghighi, (1984), Federation of American Societies for Experimental Biology, Vol. 43, p. 1530.
13) Changes in liver plasma membrane composition of cholesterol-fed rats. Thomas I. Pynadath, Ali H. Zendedel and Brian P. Maloney, (1982), Federation of American Societies for Experimental Biology, Vol. 41, p. 1613.
1) Compositions and methods for stabilizing modified tissue (# 09559933)
2) Use of dry chemistry to reduce the cost of reagents in a chemical or biochemical assay (# SV01268)
3) Apparatus and method for shrinking collagen (#200********)
4) Apparatus and method for modifying dermal collagen (#60271093)
5) Compositions and methods for the treatment of intrinsic and extrinsic skin aging changes (#60715716)
6) Methods and compositions utilizing metal cations for treatment of intrinsic & extrinsic skin aging changes (#60718534)
7) Methods and composition for generation of reactive oxygen species (#60921490)
8) Degradation of biomass by metal-catalyzed peroxide oxidation (# 61355392)
References will be provided upon request.