AARON M. L A M B E R T, P h . D.

PROFESSIONAL SUMMARY :

Versatile & innovative neurobiologist with 14+ years conducting and collaborating on integrative research across many topics, scales (molecular, cellular, circuit, behavioral), and animal models (cell culture, zebrafish, mouse, rat, monkey, human).

• Envisioned and cultivated novel doctoral dissertation distinct from – but expanding upon – lab’s central mission

• Expanded doctoral lab’s platform by developing its 1st behavioral assays and integrated them with experimental designs employing electrophysiology, transgenics, pharmacology, cellular imaging, and molecular biology

• Helped doctoral PI realize $1,500,000 in new grant funding based on novel topic and findings of doctoral dissertation

• Awarded multiple competitive fellowships from writing successful grant proposals

• Presented many invited talks, including at international conferences in front of 500+ scientists

• Collaborated and published with 10+ different labs, each from different institutions

• Co-authored 5 peer-reviewed publications (with more in-preparation), including a 1st-authorship which made the front cover of the Journal of Neuroscience and is the most cited article from doctoral lab RE S E ARCH E XP E RI E NCE (14 + YE ARS ) :

2015-2018: Harvard University, Postdoctoral Associate (Advisor: Florian Engert, Ph.D )

• Discovered that dopaminergic diencephalospinal neurons (DDNs) and endogenous dopamine receptor 4 (D4R) signaling contribute to state-dependent, goal-directed fine motor control in zebrafish, in terms of: 1) Blocking endogenous D4R signaling induces aberrantly fast and erratic spontaneous locomotion, which is suppressed in a state-dependent manner during visual detection and pursuit of prey 2) Blocking endogenous D4R signaling impairs prey capture performance specifically by inducing ineffective bradykinesia-like movements during failed attempts to advance forward toward prey, leaving visual detection and oculomotor/visuomotor prey pursuit intact in terms of effective orienting turns to reduce angle to prey 3) Optogenetic stimulation of DDNs elicits goal-directed hunting maneuvers decoupled from presence of prey

• Unveiled that zebrafish larvae are capable of visuomotor learning and long-term memory of prey capture

• Developed unbiased, automated detection and analyses of hunting sequences in free-swimming zebrafish larvae to assess goal-directed visual, oculomotor, visuomotor, motor, and locomotor strategies in the presence of prey 2009-2015: University of Minnesota- Twin Cities, Neuroscience Ph.D. Candidate (Advisor: Mark A. Masino, Ph.D )

• Elucidated that the conserved dopaminergic diencephalospinal tract (DDT) mediates vertebrate locomotor development in zebrafish by acting on D4Rs in the spinal cord, based on: 1) Correspondence in neural and behavioral developmental switch from immature to mature locomotor pattern 2) Blocking endogenous D4R signaling in vivo either before or after the developmental switch prevents or reverses the neural/behavioral switch, respectively 3) Chemogenetic ablation of orthopedia-specified DDNs perpetuates the immature locomotor pattern in vivo 4) Spinal transections of post-switch larvae reestablishes the immature neural locomotor pattern, which is rescued to a mature-like pattern via spinal D4R agonism

• Established novel adaptation of a high-throughput assay to individually track multiple zebrafish in a group arena 2008-2009: University of Minnesota- Twin Cities, Neuroscience Ph.D. Student (Advisors: Lab Rotations R1 – R4)

• R1: Seybold Lab, R2: Nick Lab, R3: Masino Lab, R4: Chafee Lab 2006-2008: Northwestern University, Research Technologist II (Advisor: Margarita L. Dubocovich, Ph.D.)

• Determined that Rozerem, a clinical melatonergic agonist, differentially regulates MT1 and MT2 melatonergic receptors in cell culture and phase-advances circadian rhythms in primates

• Maintained cell culture lines and ran radioligand receptor and G-protein binding assays, as well as GFP transfections and confocal microscopy, to elucidate mechanisms of action of melatonergic agonists 1 1 B R I S T O L S T # 2, CAMBRIDGE, MA 02141

• ( 2 1 7 ) - 649 – 4703 • ac695o@r.postjobfree.com

• Designed and ran behavioral pharmacological experiments on rhesus monkeys to investigate the potential for melatonin receptors as therapeutic targets in primates

• Genotyped mouse colonies via tail DNA extraction, PCR & gel electrophoresis

• Performed immunohistochemistry, in-situ hybridization, receptor autoradiography & western blotting 2005-2006: University of Chicago, Research Technologist I (Advisor: Peggy Mason, Ph.D.)

• Elucidated raphe magnus as a site of heterotopic antinociception; ran physiological experiments on rats’ muscle responses to both cutaneous pain (i.e. noxious heat) alone and coupled with visceral pain (i.e. colorectal distension) before and after pharmacological manipulation (i.e. muscimol or saline into raphe magnus)

• Performed surgeries, histology & data analysis pertaining to project 2004-2005: University of Illinois at Urbana-Champaign, Research Assistant (Advisor: Robert E. Wickesberg, Ph.D.)

• Demonstrated preserved temporal dynamics of auditory nerve ensemble responses to spectrally-reduced speech tokens, compared to natural speech tokens, in anaesthetized chinchillas

• Gave formal presentation of project to faculty, following semester-long course on how to give scientific talks 2004: University of Illinois at Urbana-Champaign, Research Assistant (Advisor: Gabriele Gratton, Ph.D.)

• Determined spatial resolution of an Event Related Optical Signaling (EROS) study measuring changes in optical properties of active tissue in primary visual cortex (V1) during a visual task in human subjects

• Acquired, pre-processed, and analyzed behavioral and optical data EX P ER I M EN TA L TEC H N I Q UE S E M P L O YE D:

• Behavioral Tracking: Automated Detection & Analyses of Spontaneous & Goal-Directed Behaviors During Sensorimotor & Optogenetic Manipulations (Larval Zebrafish), Locomotor & Circadian Activity Via IR sensor

(Mouse) & Actiwatch/IR Sensor/DSI Telemetry (Monkey)

• Surgeries: Stereotaxic Brain Surgery (Cannula Implantation in Rat & Zebra Finch), Dissections for Brain, Spinal Cord, & Peripheral Nerve Access (Larval Zebrafish)

• Electrophysiology: Whole-Cell & Cell-Attached Patch-Clamp (Larval Zebrafish In Vivo), Extracellular Multi-Unit (Frog Tectum in Vivo, Rat Hippocampus In Vitro, Zebrafish Spinal Nerve In Vivo & In Vitro), EMG & EKG (Rat)

• Functional & Structural Imaging: Confocal Microscopy, Genetically-Encoded Calcium Imaging (Larval Zebrafish), Event-Related Optical Signaling (Humans), Histological Sectioning (Cryostat & Microtome), Nissl Staining, Immunohistochemistry (Whole-Mount Larval Zebrafish, Mouse & Rat Coronal Sections), In-Situ Hybridization (Whole- Mount Larval Zebrafish, Mouse Coronal Sections), Receptor Autoradiography (Mouse Coronal Sections)

• Molecular Biology: DNA Extraction (Mice & Zebrafish), RNA purification (zebrafish), PCR, Quantitative Real- Time PCR, Gel Electrophoresis, Western Blotting, Antisense RNA Technology, Plasmid Microinjections (Zebrafish Single-Cell Embryos)

• Cell Culture: Multiple Lines (CHO, HEK, SCN 2.2, Primary Mouse DRGs), Transient Transfections, Radioligand Receptor Binding, GTP-γ-S Binding, Radioimmunoassays ED U C A TI O N :

2015: Ph.D. Neuroscience, University of Minnesota- Twin Cities. 104 Semester Hours earned; 3.96 Cumulative GPA

2005: BS Biological Psychology, University of Illinois- Urbana-Champaign. 152 Semester Hours earned; 3.80 Cumulative GPA

GRANTS, FELLOWSHIPS, A W A R DS, & HONORS :

2016-2021: Helped Doctoral PI realize $1,500,000+ RO1 NIH Grant Based On Novel Topic & Findings of Doctoral Work 2013-2015: Competitive Predoctoral Fellowship On MnDRIVE Neuromodulation Grant 2012: Stark Award for Advanced Scholarship- For Travel To Give Invited Talk At Specialty Conference 2010-2011: Competitive Predoctoral Fellowship On Translational Research In Neurobiology Of Disease NIH Grant Psi Chi National Honors Society Date inducted: 05/2003 Phi Theta Kappa International Honors Society Date inducted: 05/2002 ADVANCED & FUNDAMENTAL C O U R S EW O R K FOR NEUROSCIENCE : Advanced: Neurobiology of Vision, Psychophysiology of Hearing, Modeling Neural Systems, Neurobiology of Aging, Neurochemical Mechanisms of Memory, Biological Psychology, Techniques in Biological Psychology, Bio-Psych Lab, Cellular & Molecular Neuroscience, Systems Neuroscience, Developmental Neurobiology, Behavioral Neurobiology, Neural Networks, Computational Neuroscience, Neurostatistics, Fish Physiology & Behavior, Advanced Composition in Conceptual Physics, Itasca Cell & Molecular Neurobiology Lab, Career Skills & Understanding Responsibilities as a Neuroscientist Fundamental: Mathematics through Multivariable Calculus; Statistics; Full Sequences in Physics, Chemistry & Biology PU B L I C A T I ONS:

Brink TS, Hellman KM, Lambert AM, Mason P (2006). Raphe magnus neurons help protect reactions to visceral pain from interruption by cutaneous pain. J Neurophysiol 96(6):3423-32. Friedrich T, Lambert AM, Masino MA, Downes GB (2012). Mutation of zebrafish dihydrolipoamide branched chain transacylase E2 results in motor dysfunction and models maple syrup urine disease. Dis Model Mech 5(2):248-58. Lambert AM, Bonkowsky JL, Masino MA (2012). The conserved dopaminergic diencephalospinal tract mediates vertebrate locomotor development in zebrafish larvae. J Neurosci 32(39):13488-500. Cover article. 70 citations. Decker AR*, McNeill MS*, Lambert AM, Overton JD, Chen YC, Lorca RA, Johnson NA, Brockerhoff S, Mohapatra DP, MacArthur H, Panula P, Masino MA, Runnels LW, Cornell RA (2014). Abnormal differentiation of dopaminergic neurons in zebrafish trpm7 mutant larvae impairs development of the motor pattern. Dev Biol 386(2):428-39. Lambert AM (2016). Dopaminergic control of locomotor patterning during development: a tail for the ages. Front Cell Neurosci 10:95.

Lambert AM, Herrera KJ, Wee CL, Nikitchenko M, Jordi J, Bolton AB, Bahl A, Wiggin TD, Masino MA, Engert F

(In Preparation). Optogenetic and receptor-specific dissection of diencephalic dopaminergic influences to spontaneous and goal-directed behaviors in a conserved vertebrate network. Lambert AM, Bolton AB, Jordi J, Masino MA, Engert F (In Preparation). Hunting experience unveils goal-directed visuomotor learning and long-term memory in larval zebrafish. AB S T RACT S /P O S T E R S :

Tiano J, Markowska M, Lambert AM, Dubocovich M (2007). Ramelteon differentially regulates the sensitivity of hMT1 and hMT2 melatonin receptors expressed in mammalian cells. Vol 30, Abstract #1032. SLEEP; 21st Annual Meeting of the APSS. Minneapolis, MN. June, 2007.

Dubocovich ML, Lambert AM (2008). Ramelteon phase shifts circadian rhythms of activity and promotes sleep in the rhesus monkey. Experimental Biology; 100th Annual Meeting of the ASPET. San Diego, CA. April, 2008. Lambert AM, Anderson TM, Masino MA (2009). Modulation & perturbation of NMDA-induced spinal nerve activity in larval zebrafish. Graduate Program In Neuroscience Retreat. Minneapolis, MN. February, 2009. Lambert AM, Anderson TM, Masino MA (2010). Dopaminergic modulation of spinal locomotor circuits in larval zebrafish. Society for Neuroscience. San Diego, CA. November, 2010. Lambert AM, Bonkowsky JL, Masino MA (2011). The dopaminergic diencephalospinal tract mediates the developmental switch in the locomotor pattern of larval zebrafish. Society for Neuroscience. Washington, DC. November, 2011.

Lambert AM, Masino MA (2012). The dopaminergic diencephalospinal tract contributes to development of the locomotor repertoire for foraging in zebrafish larvae. Society for Neuroscience. New Orleans, LA. October, 2012. Lambert AM, Masino MA (2013). The integrities of spontaneous and hunting locomotor repertoires of zebrafish larvae are dissociably dependent on the dopaminergic diencephalospinal tract. Society for Neuroscience. San Diego, CA. November 2013.

Lambert AM, Masino MA (2014). High-throughput automated detection and analyses of hunting sequences in zebrafish larvae. Graduate Program In Neuroscience Retreat. Minneapolis, MN. February, 2014. Lambert AM, Masino MA (2015). Goal-directed visuomotor learning and long-term memory of free-swimming zebrafish larvae is unveiled via high-throughput automated detection and analyses of hunting sequences. Society for Neuroscience. Chicago, IL. October, 2015.

Lambert AM, Engert F (2016). State-dependent receptor-specific dopaminergic contributions to spontaneous and goal- directed repertoires in zebrafish. NeuroTuscany Conference on Neural Circuits & Behavior. Montecastelli, Italy. June, 2016. Schimmenti LA, Koleilat A, Lambert AM, Wiggin TD, Masino MA, Ekker SC (2017). Toward a precision medicine treatment for Usher Syndrome Type 1. 7th Strategic Conference of Zebrafish Investigators. Pacific Grove, CA. January, 2017. Koleilat A, Christenson T, Lambert AM, Bellah, JL, Masino MA, Ekker SC, Schimmenti LA (2018). Development of the first pharmacotherapy for the treatment of Usher Syndrome type 1 due to variants in MYO7a. 10th Annual Usher Syndrome Connections Conference. Mainz, Germany. July, 2018. SC I E N T I F I C T A L K S:

• The University of Minnesota; Graduate Program In Neuroscience Colloquium. Minneapolis, MN. March, 2011.

• The University of Minnesota; Graduate Program In Neuroscience Colloquium. Minneapolis, MN. February, 2012.

• The University of St. Thomas; Neuroscience Club Seminar. St. Paul, MN. April, 2012. Invited Talk.

• Carleton College; Neuroscience Capstone Seminar. Northfield, MN. April, 2012. Invited Talk.

• 11th International Conference on Zebrafish Development and Genetics. Madison, WI. June, 2012. Invited Talk.

• College of St. Benedict/St. John’s University. St. Joseph, MN. October, 2012. Invited Talk.

• The University of Minnesota; Graduate Program In Neuroscience Colloquium. Minneapolis, MN. February, 2013.

• The University of Minnesota; Graduate Program In Neuroscience Colloquium. Minneapolis, MN. April, 2014.

• The University of Minnesota; Graduate Program In Neuroscience. Minneapolis, MN. February, 2015. Thesis Defense.

• NeuroTuscany Conference on Neural Circuits and Behavior. Montecastelli, Italy. June, 2016. Invited Talk.

• Harvard University; Biological Laboratories. Cambridge, MA. March, 2017.

• Harvard Medical School; Center for Life Sciences. Boston, MA. August, 2018. REFERENCES:

• Dr. Florian Engert, Harvard University, Professor of Molecular and Cellular Biology, ac695o@r.postjobfree.com

• Dr. James Ashe, University of Minnesota- Twin Cities, Professor of Neuroscience, ac695o@r.postjobfree.com

• Dr. Teresa Nick, McKinsey & Company, Digital Expert, ac695o@r.postjobfree.com ac695o@r.postjobfree.com

Contact this candidate