Microsoft Office Manager

Profile

Earned a Ph. D in cell biology and has post-doctoral experience at USC Keck School of Medicine in the area of signal transduction of receptors, protein kinases, and cAMP in neuroprotection and cancer chemoprevention. Obtained advanced experience in the areas of cell biology, molecular biology, cancer biology, biochemical, and imaging techniques to screen new molecules and drugs for potential therapeutic applications to cancer and neurological diseases. Received first and co-authorship of several publications. Has experience working independently and collaboratively across various disciplines. Gained over eight years of research experience while training students.

Key Words: Cell Culture, Immunofluorescence, Confocal imaging, Cell Biology, Cryopreservation, q-PCR, ELISA, Western blots, Native PAGE gels, small animal dissections, Microsoft office, Analysis of data and Scientific publications.

Areas of Expertise

Culturing several cell lines normal, established cancer and neuronal cells, and three -dimensional explants.

Isolating primary hippocampal stem cells and dissociated cortical neurons.

Cell imaging by using confocal, bright field and fluorescence microscopy and time lapse video compilations.

Double and triple immunofluorescence Intensity and colocalization analysis.

Immuno- histochemical staining of tissue sections and cells.

DNA and RNA isolation from cells and tissues. Genomic PCR, Conventional PCR, Quantitative PCR and ISH.

DNA, RNA transfection, RNAi (with siRNA and shRNA), flow cytometry

Protein extractions, western immune analysis, Dot blots, native PAGE gel electrophoresis of media and tissues, in vitro and in vivo radiolabeling of proteins & efflux experiments.

ELISA, high throughput screening assays, fluorescence and colorimetric luminometers and microplate reader by using soft-max software.

Mouse and rat dissections, genotyping and mice breeding.

Taken lab safety, blood-borne pathogen, radioactive and animal trainings.

Writing animal protocols for IACUC.

Research and data analysis

Policy Implementation

Time Management

Effective Multitasking

Microsoft Word, Excel, PowerPoint, Pubmed, google scholar for literature search, Endnote reference manager.

Adobe Photoshop, Adobe Illustrator.

Image J, Leica confocal image software, metamorph image software for neurite measurement, softmax and Graph Pad Prism 5.

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Professional Experience

Research Associate

November 2017- Present

P.I: Hussein Yassine, M.D

ApoE4 Aggregation and hypo lipidation is implicated in Alzheimer’s Disease Pathology in Cellular, Animal and Human Studies

February 2004 to October 2017

Keck School of Medicine, USC Los Angeles, CA

P.I: Thomas H. Mc Neill, Ph.D.

Isolating synaptoneurosomes for quantitation of NMDA receptor subunits and its phosphorylation

Receptor-mediated signal transduction in neuroprotection and neurodegeneration.

P.I: Rick A. Friedman, M.D., Ph.D

Role of Neuropilin-1/Semaphorin-3A signaling in the functional and morphological integrity of the cochlea.

Post –doctoral Fellow

Sept 2003 – 2004 November

P.I: Rajesh Gaur, Ph.D.

Beckman Research Institute, City of Hope, Duarte, CA

Investigated role of CEA in breast cancer using RNAi method

Trained student researchers in cell and molecular biology techniques

Assist PI with grant proposals to secure funding.

Education

Ph.D. - Osmania University Hyderabad

IICT India, Graduated

NIH- Visiting Fellow:

NIEHS Research Triangle Park, NC, USA

Awards and Honors

Best Poster of the Session, XVI International Cancer Congress, (1994) Tamoxifen and Oxidative Stress

Brigid Leventhal Trainee Award, American Institute of Cancer Research, (1996) Received for JBC abstract (1996) 271, 135**-*****.

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=Gundimeda+U

Citizenship

U.S. Citizen

Memberships

1.Society for Neuroscience - Member

2.American Association of Cancer Research – Member

3.Society of Toxicology – Member

References:

Will be provided upon request.

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