DAS, BISWAJIT

***** ****** **** *****, ***. ***, Willoughby Hills, OH, 44092.

Phone: +1-216-***-**** e.mail: ac603s@r.postjobfree.com Accomplishments

Developed and performed cellular gene target identification through shRNA library screening (both global and focused on epigenetic modifiers), validation and per-clinical studies in human cell based assay, re-purposing of FDA approved drugs towards newly identified drug targets in Human cell lines, in primary cell model and in patient samples.

Developed three HIV-1 latently infected human monocyte cell lines and validated through re-activation of provirus with known stimulus.

Experienced in human cell based assay to determine toxicity and LD50 values of newly identified small molecules as a key component of pharmaceutical drugs (collaborative work with MERCK)

Published original research work at various internationally reputed Scientific Journals like Proceedings of National Academic of Science, USA., Science Report., Virology, Molecular Biology, Retro Virology, Journal of Biological Chemist etc.

Patent applied (awaiting release): US patent on "Induction and Suppression of Latent HIV Proviruses by Estrogen Receptor-Alpha (ESR-1) Antagonists And Agonists" by Case No. 2013-2380.

Testing: Coordinated data analysis and provided recommendations based on test results.

Experimental design: Strategy development for shRNA library screening, potential target identification and validation.

Product Development: Re-purposing of FDA approved breast cancer drugs in latency reversal of HIV-1 infection. Professional Summary

Cellular and Molecular Biologist with more than 15 years of research experience in the field of Signaling biology, Molecular Immunology and virology, HIV research, microbiology, microRNA biology, stress and cardiovascular biology interested to pursue research expertise to explore the scientific thoughts towards drug developmental and/or per-clinical studies for establishing acceptable strategy to cure life threatening diseases. Skills

Molecular Biology:

DNA and RNA isolation, Primer designing, PCR

amplification of gene, RT-PCR, quantitative Real Time PCR, Electrophoretic Mobility Shift Assay (EMSA),

DNA mutagenesis, Northern blot analysis.

Construction of shRNA-mir library.

Protein Biology:

Expression of recombinant gene in prokaryotic

system, SDS-PAGE, Western Blot analysis, Immuno-

precipitation, GST pull-down assay.

Isolation, purification and characterization of protein, Primary cell based bio-assay of functionally active

Screening of shRNA library and data analysis of High Throughput-sequencing.

ChIP analysis. EDITs assay (newly developed at

Laboratory of Jonathan Karn)

Fluorescence based multi-plex Transcription Factor array.

Designing / construction of vectors, Bacterial cloning and Transformation, Restriction Analysis, Generation of mutants, transcript profiling and it's analysis, isolation of genes/proteins of interest by expression cloning, construction of plant expression vectors, microRNA profiling, designing of microRNA-mimic and AntagomiR.

Stable knock-down of target genes by introducing shRNA and CRISPR technology.

Cell Bilogy:

Flow cytrometry and Flow based cell sorting

Image stream flow analysis

Nested PCR analysis for identification of integrated shRNA in host genome.

Isolation of Monocyte and T-cell from human

peripheral blood, polarization of T-cells in different sub-sets.

Preparation of High titer Lenti virus and AAV particle with shRNA or CRISPR or mammalian over

expression cassette.

protein.

Preparation of functionally active fluorophore

conjugated protein.

Generation mutant protein, In vivo interaction assay.

Histone purification and analysis of Mass

spectrometry data.

Immuno-Histology / histology:

Confocal microscopy and image analysis, Cell based trafficking of protein molecules, Scanning and

Transmission Electron Microscopy, immuno-

histochemistry of tissue sections.

Tissue / cell culture:

Experienced in primary cell culture (cardiac myocytes, Human T-cell) and other cell lines like HEK-293, HL-1, Jurkat, THP1 monocyte, U-937, SC and MD

monocyte-macrophage cell line.

Skilled in in vitro culture of plant tissues and Agrobacterium mediated plant transformation.

Bioinformatics:

Familiar with all commonly used software to analyze gene, protein and microRNA like all kind of BLAST

analysis, Genomatix, Ingenuity Pathway AnalysisTM, String analysis, GeneMania on Cytoscape, ExPASy,

Multalin, CLASTAL W, PyMol, TargetScan, PicTar,

RNA22, DIANA microT, STarMir, RNAHybrid,

miRanda, Image J analysis etc.

Computer knowledge: Microsoft office (MS word, Excel, Power point etc), Adode photoshop, Adobe Illustrator & imdesign, Origin, Endnote.

Other:

Gas Liquid Chromatography, Experienced in mice

handling and mice based research work.

Work History

Senior Research Associate 2011 - 2018

Case Western Reserve University School of Medicine Cleveland, USA, Ohio

Recipient of Best Research Work Poster Award at the Center for AIDS Research (CFAR) Internal Scientific Retreat – 2014 held on February 18, 2014 at Case Western Reserve University, Cleveland, Ohio, USA CWRU, Cleveland, Ohio, USA.

Recipient of Best Research Work Poster Award at the Center for AIDS Research (CFAR) Internal Scientific Retreat – 2013 held on January 31, 2013 at Case Western Reserve University, Cleveland, Ohio, USA CWRU, Cleveland, Ohio, USA.

Recipient of Travel Award from National Institute of Health, U.S. Department of Health and Human Services at the

“Chromatin Control of Viral Infection Workshop” held on September 17-18, 2012 At the Natcher Conference Center, NIH, Bethesda, Maryland, USA.

Recipient of Best Research Work Poster Award at the 5th Biennial Research Retreat of Department of Molecular Biology & Microbiology, Case Western Reserve University< Cleveland, Ohio, USA held on September 6-7, 2012 at CWRU, Cleveland, Ohio, USA.

Adjunct Staff Jan 2011 - Dec 2011

Lerner Research Institute, Cleveland Clinic Cleveland, Ohio Post Doctoral Research Fellow Jun 2005 - Dec 2010

Lerner Research Institute, Cleveland Clinic Ohio

Recipient of New Investigator Travel Award from American Heart Association Council on Basic Cardiovascular Sciences at the 3rd Annual Symposium on “Translation of Basic Insights into Clinical Practice”, held on July 31 – August 3, 2006, at the Keystone Conference Center, in Keystone, Colorado, USA. Research Associate Jun 2000 - May 2005

Plant Molecular and Cellular Genetics, Bose Institute Calcutta, India Contribution as Senior Research Associate and Post Doctoral Fellow Jun 2005 - Current Case Western Reserve University and LRI, Cleveland Clonic Cleveland, Ohio

Designed and performed laboratory based biological experiments, analyzed acquired data, and resolve technical difficulties, if any.

On completion of project targets the outcome was compiled in the form of manuscript to publish in scientific journals and also to submit as a new project proposal to various funding agencies.

Worked in collaboration with MERCK infectious disease section, Pennsylvania, USA for testing various potent small molecules in cell based assay to validate the efficacy of those molecules against a specific target and determined the best possible doses to obtain optimum effect.

Education

Ph.D.: Plant Physiology 2000

University of Calcutta, Inidia

Master of Science: Botany (Specialization: Environmental Biology) 1992 School of Science, Visva-Bharati University, India Ranked obtained: 1st

Scored at Graduate Aptitude Test in Engineering (Life Sciences) in the year 1993 for Research Fellowship awarded by Human Resource Development Ministry, Govt. of India.

Awarded National Scholarship for the year 1990-1991 by the Ministry of Human Resource Development

(Department of Education), Government of India for outstanding performance.

Awarded Visva Bharati ‘University Merit Scholarship' consecutively from 1990 to 1992. Bachelor of Science: Life Science (Minor subject: Physics and Chemistry) 1990 School of Science, Visva-BHarati University, India Rank obtained : 1st

Awarded National Scholarship for the year 1987-1990 by the Ministry of Human Resource Development

(Department of Education), Government of India for outstanding performance.

Awarded Visva Bharati ‘University Merit Scholarship' consecutively from 1987 to 1992. Personal Information

Date of Birth: 27th June, 1969

Citizenship: Indian

Current Immigration Status at USA: USA permanent Resident SOFT SKILL PROFILE

Hard working and motivated research scientist.

Highly adept in working in large or small groups.

Guided junior researchers and rotation students a number of times throughout my PhD and post-doctoral tenure.

Capable of multitasking, prioritizing and analyzing a wide variety of data.

Always open to ideas, eager to learn new techniques.

Amicable person, glad to help and troubleshoot for colleagues.

Excellent communication skills.

References

Jonathan Karn, Ph.D.

Reinberger Professor of Molecular Biology

Chairman: Department of Molecular Biology and

Microbiology,

Director: CWRU Center for AIDS Research.

Case Western Reserve University SChool of Medicine 10900 Euclid Avenue

Cleveland, OH, 44106

Phone: +1-216-***-****

Fax: +1-216-***-****

e.mail: ac603s@r.postjobfree.com

Richard J.O. Barnard Ph.D

Director, Global Regulatory Affairs and Clinical Safety

(Infectious Disease)

Mail Stop UG2D-68

351 N. Sumneytown Pike

North Wales, PA, 19454

Phone: +1-267-***-****

Fax: +1-267-***-****

e.mail: ac603s@r.postjobfree.com

Kaushik Ghosal, Ph.D

Director, Business Development

BioMotiv, 20600 Chagrin Blvd.,

Cleveland, OH, 44122

Phone:+1-216-***-****

e.mail: ac603s@r.postjobfree.com

Affiliations

American Heart Association, USA. From January 2006 to December 2010 American Association for the Advancement of Science, USA. From January 2012 to January 2014 Publications

1. Das B, Dobrowolski C, Luttge B, Valadkhan S, Chomont N, Johnston R, Bacchetti P, Hoh R, Gandhi M, Deeks SG, Scully E, Karn J. (2018). Estrogen receptor-1 is a key regulator of HIV-1 latency that imparts gender-specific restrictions on the latent reservoir. Proc Natl Acad Sci U S A. (doi:10.1073/pnas.180*******.) 2. Nguyen K, Das B, Dobrowolski C, Karn J. (2017). Multiple Histone Lysine Methyltransferases Are Required for the Establishment and Maintenance of HIV-1 Latency. M. Bio., Feb. 28,8(1). pii: e00133-17. doi: 10.1128/mBio.00133-17

3. Alvarez-Carbonell D, Garcia-Mesa Y, Milne S, Das B, Dobrowolski C, Rojas R, Karn J. (2017). Toll-like receptor 3 activation selectively reverses HIV latency in microglial cells. Retrovirology. 2017 Feb 6;14(1):9. doi: 10.1186/s12977-017-0335-8.

4. Li Q, Karim AF, Ding X, Das B, Dobrowolski C, Gibson RM, Quiñones-Mateu ME, Karn J, Rojas RE. (2016) Novel high throughput pooled shRNA screening identifies NQO1 as a potential drug target for host directed therapy for tuberculosis. Science Rep. Jun 14;6:27566. doi: 10.1038/srep27566 5. Yu X, Shahir AM, Sha J, Feng Z, Eapen B, Nithianantham S, Das B, Karn J, Weinberg A, Bissada NF, Ye F.

(2014) Short Chain Fatty Acids From Periodontal Pathogens Suppress HDACs, EZH2, and SUV39H1 to Promote Kaposi's Sarcoma-Associated Herpesvirus Replication. Virol., 88:4466-4479. 6. Das B, Young D, Vasanji A, Gupta S, Sarkar S, Sen S. (2010) Influence of p53 in the transition of myotrophin- induced cardiac hypertrophy to heart failure. Cardiovascular Research., 87(3):524-534. 7. Gupta A, Gupta S, Young D, Das B, McMahon J and Sen S. (2010) Impairment of ultrastructure and cytoskeleton during progression of cardiac hypertrophy to heart failure. Laboratory Investigation, 90(4):520-530. 8. Das B, Gupta S, Vasanji A, Xu Z, Misra S and Sen S. (2008). Nuclear co-translocation of myotrophin and p65 stimulates myocyte growth: regulation by myotrophin hairpin loops. J. Biol. Chem., 283(41): 27947-56. 9. Reddy KB, Fox JEB, Price MG, Kulkarni S, Gupta S, Das B and Smith DM. (2008). Nuclear Localization of Myomesin-1: possible functions. J. Muscle. Res. Cell. Motil., 29: 1-8. 10. Gupta S, Das B and Sen S. (2007). Cardiac hypertrophy: Mechanisms and Therapeutic opportunities. Antioxidant and Redox Signaling, 9(6): 623-652.

11. Das B, Roy S, Gangopadhyay G, Poddar R and Saha PK. (2006) Testa ultrastructures, water uptake pattern and seed germination of some medicinally important plants of Fabaceae. J. Bot. Soc. Bengal., 60(1): 50-64. 12. Das B, Goswami L, Ray S, Ghosh S, Bhattacharyya S, Das S, Majumder AL. (2006) Agrobacterium-mediated transformation of Brassica juncea with a cyanobacterial (Synechocystis PCC6803) delta-6-desaturase gene leads to production of gamma-linolenic acid. Plant Cell Tissue & Organ Culture, 86: 219-231. 13. Das B and Saha, PK. (2006) Ultrastuctural dimorphism of micropyle determines differential germinability of Sesbania cannabina seeds. Seed Science & Technology, 34:363-372. 14. Dutta S, Das B, Bhattacharya S. (2002) Selection of a chemical treatment effecting enhancement of germination of Abroma augusra Linn. seeds. Seed Science & Technolog, 30:659–662. 15. Bhattacharyya S, Das B, Ghose TK, Bhattacharya S. (1999) Investigation on germination in relation to the total phenol content in the seeds of Nyctanthes arbor-tristis (Oleceae). Seed Science & Technology, 27:321–327. 16. Bhattacharya A, Das B, Saha PK. (1999) Qualitative and quantitative estimation of indole-3-acetic acid (IAA) in the seeds of Shorea robusta Gaertn. F. J of Tropical Forest Science, 11:528–536. 17. Das B, Saha PK. (1999) Effect of dormany breaking treatments on testa ultrastructures and water uptake patterns of Albizia procera seed. Seed Science & Technology, 27:615–625. 18. Biswas S, Das B, Saha PK. (1997) Dormancy status as influenced by hilum in phenotypically characterized seeds of Pachyrhizus angulatus Rich. Ex. Dc. and breaking of dormancy. J Natl Botanical Society, 51:53–60. 19. Saha PK, Biswas S, Das B, Ganguly SN. (1995) Studies on seed viability of Shorea robusta Gaertn. f. J Non- Tomber Forest Products, 2:61–62.

Books chapter:

Das B, Bhattacharya A, Saha PK, Chetti MB, Hiremath SM. (1997) Water uptake patterns in Cassia tora L. as influenced by different seed types. In: First International Conference on Parthenium, Vol II (eds. M. Mahdevappa and V.C.Patil), University of Agricultural Science, Darwar, Karnataka. Viswakala Printers,, pp. 70–73.

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