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Medical Data

Livermore, California, United States
June 21, 2018

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Gian P. Ralph-Nakoinz

**** ********* *** *********: 530-***-****

Livermore, CA 94551 e-Mail:


Seeking to leverage and expand my capabilities of assay development in GxP environment at a high impact biotech pharma enterprise.


Senior Research Associate III: POC Medical Systems Mar 2017-present

• Working with human plasma in BSL II environment to both run ELISA and develop proprietary assays on biomarkers

• Analyzing reagents using SEC-HPLC, NanoDrop, BioAnalyzer, IEX chromatography.

• Chemically conjugating antibodies and proteins on to silica beads and R-PE reporter protein.

• Purifying small scale preparations of antibodies, proteins and other molecules.

• Characterizing antibody affinity using BLiTZ instrument.

• Epitope binning experiments; Protein unfolding experiments

• Formulating lyophilization buffers and troubleshooting lyophilization protocols for manufacture

• Troubleshooting microfluidic devices

• Maintaining and upkeep of instruments used in Research Group. Ordering/shipping/receiving products used in Research.

Senior Research Associate: Marin Biologic Laboratories 2010 - 2012, 2016 - 2017

Running ELISAs, bioassays, enzymatic assays, as well as spectrometry, SDS-PAGE, western blots, and protein quantitation

Freezing, thawing, and maintaining various mammalian cell lines

Isolating leukocytes and plasma from human whole blood and subsequent assays including ADCC

Writing reports, troubleshooting experiments, and designing protocols from internet searches and literature

Training new employees. Collaborating projects with multiple supervisors, contemporaries and clients

Surgically isolating hepatocyte and other cells from rat and hamster, as well as husbandry of those animals.

Ensuring GMP/GLP compliance, Performing Quality Control/Quality Assurance duties, SOP authoring and revision. Checking paperwork, and initiating CAPA, DCR, investigations, and general compliance

Analyzing data and some programming: Excel, Prism, SoftMax, and others

GxP ordering/receiving/shipping; maintaining lab FDA compliance; calibrating, maintaining, and troubleshooting equipment

ELISA Consultant: Exoxemis Spring - Fall 2014

Running ELISA's on clinical samples

Assistant Level I: Gubler Lab, Department of Plant Pathology, UC Davis Spring 2008

Preparing PCR samples and assisting with PCR analysis

Collecting spore trap data

Intern: Krubitzer Lab, Department of Psychology, UC Davis Winter 2007

Preparing and digitalization of slides of auditory neurotracer studies in small rodents

Observing with slight assistance the neuroanatomical surgeries for the slides

Intern: Medical Psychiatry Department of Psychology, Center for Neuroscience, UC Davis Spring 2006

Immunohistochemical staining of neocortex tissues to isolate inhibitory pyramidal cells for PCR analysis


* HPLC * Gel Filtration * Tissue Culture * ELISA * GxP/FDA Compliance * Data Analysis * Leadership *

* QA/QC * Computer Programming * Protein Chemistry * Radiation Safety * Laboratory Management *

* Spectrometry * Report Writing * Troubleshooting * Purifications * Enzymatic Assays * Binding and Binning Assays *


University of California, Davis, B.S. Neuroscience, Physiology, and Biology June 2009

Minor in Psychology

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