Assistant Research

Sujata Parajuli

**** ****** ***. *** **** East Norriton, PA 19401

*** -*** – 8914

***************@*******.***

OBJECTIVE; To obtain a position in a research laboratory using critical thinking and teamwork skills plus the ability to work independently.

EDUCATION

B.S in Biology, GPA 3.2

Rosemont College, Rosemont PA 19010

RESEARCH EXPERIENCE Nov 2017-present

Merck &Co Inc.

Associate Scientist

-Supported varicella bulk vaccine production using flow cytometry antigen expression assay.

-Troubleshoot and worked independently to support the assay.

-Prepared laboratory reagents, media and solutions.

-Performed lab –scale upstream processing of vaccine products and process intermediates.

-Analyzed, tracked and maintained records tests results following good laboratory practices.

-Performed routine calculations and maintained appropriate log books and note books.

- Used and maintained laboratory equipment’s such as vi-cell to perform the cell count.

Fujirebio Diagnostics Inc.

Laboratory technician. May 2017- Nov 2017

-Prepared solutions, buffers and measured the Ph of solutions.

-Maintained cGMP and GLP compliance, revised SOPS and batch records.

-Used equipment such as mixers, ph meters, filtration apparatus, laminar flow hood, centrifuges and vacuum pumps.

-Used immunoassays to detect a specific substance in a blood or human serum plasma.

Rosemont College Department of Biology, Rosemont, PA Aug.2015-Jan.2017

Undergraduate Research Assistant for Professor Aikaterini. Skokotas

-Conducted research in DNA fingerprinting analysis by using restriction enzymes.

-Extracted DNA from human cell using Gel Electrophoresis

-Conducted research in DNA isolation, western blot, plasmid synthesis, primer design and sub cloning.

-Prepared Southern blot to transfer the electrophoresis- separated DNA fragments and hybridized the immobilized DNA.

-Prepared genomic DNA from bacteria and amplified using Polymerase Chain Reaction.

-Used pure culture techniques such as streak plate method, spread plate method, and pour plate method to determine the characteristics of a microorganism.

SKILLS

Technology: Track wise software, Quality Data Interchange, Word, Excel, PowerPoint.

Language: Fluent in Nepali (Native language) and English, Proficient in Hindi, Basic knowledge of Spanish.

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