Technical Assistant
Resume:
Pannaga Parthasarathy Tel: 510-***-****
**** ********* ***** *****: **********@*****.***
Fremont CA 94555
Summary:
Accomplished and well organized biopharma professional with a background in Molecular Biology and Immunology. Skilled in most major techniques used in the biopharma industry with excellent interpersonal and communication skills. A quick learner who consistently exceeds expectations in demonstrating technical skills.
Career History:
2014- present : Caring for my son who got diagnosed with Crohn's.
Abbott/Abbvie, Redwood City, CA 2010 – 2013
Development Associate Scientist
Key Accomplishments:
Developed methods and Assayed samples using ELISAs to determine potency of various bioprocess samples. Assayed samples using ADCC method to determine its cytotoxicity.
Assayed samples using Proliferation Inhibition method to determine the relative potency.
Qualified ELISA methods for transfer to national and international sites.
Took initiatives to optimize analytical techniques with the scientists involved.
Demonstrated a high degree of productivity under a stringent time frame to meet DS/DP release timelines with reduced staff.
Technical skills Acquired: ELISA, Radio isotope labeling cells with Cr51, Cell culture, separation of PBMC from whole blood.
PDL Biopharma, Redwood City, CA 2006 – 2008
Development Associate
Key Accomplishments:
Assayed samples using ELISAs to measure their potency.
Assayed samples using ADCC method to determine their cytotoxicity.
Assayed samples using Proliferation Inhibition method to determine their relative potency. Participated in method qualification for determination of the Bio identity of a monoclonal antibody by its Anti Id bead assay.
Took initiatives to optimize analytical techniques with the scientists involved.
Provided editorial assistance with development test method SOPs.
Demonstrated a high degree of productivity under a stringent time frame to meet DS/DP release timelines with reduced staff.
Training:
GLP and GMP
Conflict management
Technical Skills Acquired: ELISA, Radio isotope labeling cells with Cr51, Cell culture.
Aushad LLC, 2005 – 2005
Consultant
Key Accomplishments:
Reported adverse events from drug trial reports.
Reviewed documents to categorize the severity of adverse events
Ensured that the events were reported in accordance with FDA regulations
Learnt about the documentation procedures regarding adverse and serious adverse reactions during clinical trial of an investigative drug.
Abgenix, Fremont, CA 2002 – 2004
Research Associate
Key Accomplishments:
Assayed cell supes as well as purified samples for protein concentration.
Assayed protein samples for their potency by ELISA
Screened the cell clones and sub clones to determine the protein expression levels
Assayed the integrity of the protein samples by Gel Electrophoresis.
Developed ELISAs based on requirement to determine the potency of monoclonal
antibodies.
Developed and screened formulations for fully human therapeutic recombinant antibodies.
Assayed proteins for the presence of endotoxin by LAL
Assayed the purified protein samples for DNA contamination
Analyzed monoclonal antibody samples for aggregates and fragments using HPLC and SDS PAGE methods
Evaluated a suitable filter for the filtration of IgM, such that the integrity and the
pentameric structure was maintained. The evaluated filter was employed in the viral
validation studies.
Contributed towards a research paper on the filtration studies of IgM antibody.
Prepared SOPs, technical summaries and reports.
Technical Skills Acquired: ELISA, SDS PAGE, HPLC.
St. John’s Medical College, Bangalore, INDIA 1994 – 1996
Research Assistant
Key Accomplishments:
Subjected Albino mice to hypoxic conditions. After a preset period of time, they were sacrificed, and the organs were harvested for study.
The tissues obtained were homogenized and the sub cellular particles were separated.
The isolated proteins of interest were studied by Electrophoresis, ELISA, Chromatography, and Western Blot techniques.
Topic of Research: Effect of Hypoxia on Cytosolic and Mitochondrial Enzymes.
Result of Study:
Was able to show an increased activity of the Cytosolic enzyme, Lactate Dehydrogenase.
Mentored and supervised undergraduate students in their laboratory and project work as part of Teaching Assistants job responsibility
Technical Skills Acquired: ELISA, Electrophoresis, Chromatography, Western Blot.
Education:
BS (Biology) - Gulbarga University, India
MS (Biochemistry, Molecular biology and Immunology) - Gulbarga University, India
Certificate course (Bioinformatics) - UCSC, California
Course study (Stem Cell Biology) - UCSC, California
Awards:
Awarded the University Grants Commission Junior Fellowship to pursue Doctoral studies in India.
As a member of The Association of Clinical Biochemists of India, presented a poster on the topic of research – Effect of Hypoxia on Cytosolic enzymes– to show an increased activity of the enzyme Lactate Dehydrogenase (LDH).
Contributed towards a paper establishing the “Influence of Histidine on the stability and physical properties of a fully human antibody in aqueous and solid forms”.
Bei Chen, Raquel Bautista, Kwok Yu, Gerarado A. Zapata, Michael G. Mulkerrin, Steven M. Chamow.
REFERENCES: (Available upon request)
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