Biotechnology, Drug Manufacturing, Clinical Research

Skills:

Word, Excel, Power Point, hands-on SAP (Astrazeneca), SoftMax Pro, Unicorn, Delta V, QC: MALDI-TOF MS, CE, Spectrophotometer, Plate-reader; Bioprocess Equipment: AKTA, GE/Pall Skids, Column-packing pump; TFF/Cross-flow, Virus inactivation/Depth Filtration

Education:

Johns Hopkins University, Baltimore, Maryland

M.S., Biotechnology;

University of Liverpool, UK

Post-Graduate Diploma Clinical Research Administration

Howard University, Washington, D.C

B.S., Chemistry

Employment History:

Paragon Bioservices Baltimore, Maryland June 2016 - April 2018

Drug Manufacturing Associate II

Client-Specific Drug Manufacturing Processes involving:

Downstream protein purification operations- AKTA process equipment

Concentration/diafiltration - TFF/Cross-Flow

Clean/Store TFF/Cross-Flow

Central Services - Buffer Prep for all departments

Assemble/Autoclave materials as desired

Manual Cleaning – small parts

Saudi Academy Alexandria, VA Sept 2015 – June 2016

Stem Teacher

Teach Grades 7-12 - Chemistry, Physical Science, Biology; facilitate classroom engagement activities and laboratories with science students.

Washington Christian Academy Olney MD Sept 2014 - June 2015

Stem Teacher

Teach Grades 9-12 - General Biology, Human Anatomy & Physiology, and Psychology; Support AP Biology classes/ labs

Astra-Zeneca/Medimmune Frederick, MD Dec 2011 - Sept 2014

Drug Manufacturing Production Technician III

Large scale protein purification (monoclonal antibodies)

Operate Bioprocess Equipment: Pall/GE Chromatography Skids, Virus Filtration Skids

Participate in large scale column-packing processes using column-packing equipment

Execute CIP/SIP of process vessels such as stainless-steel tanks, transfer panels/lines

Real-time process monitoring: UV, pH/Conductivity, pressure indicators

Execute Large Scale Buffer preparations per SPRs

Operate Labware Washers and Autoclaves

Human Genome Sciences Rockville, MD Feb 2007 – Dec 2011

Drug Manufacturing Associate III

Execute large scale protein purification (monoclonal antibodies)

Operate Bioprocess Equipment: GE Chromatography Skids, Virus Filtration Skids, UF-15500 Skids (TFF)

Real-time process monitoring: UV, pH/Conductivity, pressure indicators

Virus Inactivation/Depth Filtration

Execute CIP/SIP of process vessels such as stainless-steel tanks, transfer panels/lines

Assist with Bulk Drug filling operations as required

Audit and order new logbooks

Write and Edit Batch Records/SOPs

Train new employees as required

Montgomery College Gaithersburg, MD Aug 2005- May 2008

Adjunct Professor of Biology

General Biology and Principles of Biology I (Lecture and Lab)

Baxter Healthcare Beltsville, MD Mar 2006 - Feb 2007

Vaccine Manufacturing Associate II

Protein purification for vaccine manufacturing (Acellular pertussis, Mening C);

Process Equipment used: chromatography affinity columns (BS, FS) and G-25 size-exclusion column

Operon Biotechnologies Germantown, MD Nov 2004 - Mar 2006

Team Lead

Coordinate high throughput oligonucleotide production, purification, cleavage and

deprotection

Prepare reagents and add base modification to synthesizers as required during processing.

Identify oligos from database and prepare for synthesis, processing and shipping

Desalt genome oligos and special orders

Tech transfer high throughput oligonucleotide production technology from the Alameda, California facility to Germantown, Maryland.

Train new employees as required

Qiagen Sciences Germantown, MD Nov 2002 – Jul 2004

Senior Drug Manufacturing Associate

Participate in oligonucleotide productions start up.

Oligonucleotide Synthesis - DNA

Provide support to Oligonucleotide production team.

DNA synthesis platforms, performed validation, edited and wrote SOPs

Other assigned duties

Invitrogen-Life Technologies Frederick, MD Jul 1999 – Oct 2001

Associate Scientist II

Team-lead (1999-2000): Supervised oligonucleotide production team

Synthesize oligonucleotides using high-throughput systems platforms

Plate-reader, SoftMaxPro

Quality Control: MALDI-TOF MS, capillary electrophoresis (CE)

Very pass/fail oligonucleotide from CE data

New employees training, SOP editing and other lab support functions

DNA Technologies, Inc. Gaithersburg, MD Mar 1998 - Jul 1999

Research Associate

Perform Western Blots (Single and Multiple tissues, Cell Lines)

Large Scale Plasmid Isolation (100-500mg)

Protein expression utilizing restriction enzymes and E. coli (Pvu II, Hind III, EcoRI),

Recombinant DNA techniques involving the use of competent cells and protein expression.

Reagent, Buffer and Media preparations

American Type Culture Collection (ATCC) Manassas, VA Feb 1995 - Mar 1998

Biologist

Perform Organic and Inorganic DNA extraction and purification

Isolate lymphocytes from whole blood for EBV transformation (ficoll application)

RNA-free DNA agarose Gel Electrophoresis

Cryopreservation of whole blood using 10% DMSO and Liquid Nitrogen

Feeder Layer preparation

Manual Cell Counts - Hemocytometer and cell viability determination (methylene blue)

Reagent and buffer preparations (TAE, TE, SDS, Triton X-100)

Record keeping of incoming laboratory samples

Prepare hazardous materials for shipment

Beaumont Comprehensive High School Manchester, Jamaica 1981 – 1984

High School Teacher

Chemistry, Physics and General Math: (Algebra, Arithmetic and Geometry).

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