Senior Research Associate
Resume:
Nichole T Franz, MSc.
**** ** ********* **, ***’s Summit, MO 64082
816-***-**** *************@*****.***
OBJECTIVE
Scientist position that may lead to future growth within a company. QUALIFICATIONS
Highly skilled Research Scientist with nearly 18 years of experience in the development and execution of complex experimental protocols for use in scientific journal publications and validations for clinical laboratory testing. Skilled in effectively developing experimental plans in order to conduct research assays which meet developmental requirements fulfilling client specified timelines and corporate derived cost goals, data analysis, data interpretation and trouble shooting. Successful at producing accurate and reproducible results of both scientific journal and medical diagnostic quality. PROFESSIONAL BACKGROUND
Senior Research Associate – Proteomics and Mass Spectrometry Facility 2017 Present University of Missouri – Kansas City
In gel trypsin digestion and FASP (filter aided sample preparation)
Solid phase extraction of digests
Sample immunodepletion using Multiple Affinity Removal System LC column
Whole peptidome analysis using multidimensional liquid chromatography (mudpit) using Eksigent nanoLC and CTC PAL robot for fraction collection
Database searches using Proteome Discoverer and Mascot
Nano LC 2D using the Eksigent nano LC pump system and Mass Spectrometry using the Thermo Q Exactive Plus (with Thermo Finnigan LTQ linear ion trap) mass spectrometer (LC MS/MS) Senior Associate Scientist – Large Molecule
2015 2017 Catalent Pharma Solutions
Experience with cGMP and an understanding of biopharmaceutical regulatory environment
Familiar with US FDA guidelines 21.CFR, and ICH guidelines for method validation
Good Documentation Practices
Data review/peer review
Technical writing of SOPs, client notifications, scientific documentations, and project reports
Experience in mammalian cell culture and sterile technique
Microplate assays (96 well) using microplate readers (some combination of visible, fluorescent and/or luminescent readers)
Proficient analyst with experience in all of the following: o In vitro cell based bioassays
o Execution and analysis
o Troubleshooting
o Development and/or optimization
o ELISA assays/immunochemistry for potency, binding affinity, enzyme activity and enzyme kinetics o Execution and analysis
o Troubleshooting
o Development and/or optimization
o Molecular biology/PCR/qPCR/RT qPCR
Ability to multitask
Ability to be flexible
Team player
Executes projects on timely basis
Mentor/train peers
Regular Communication with:
o Members of staff within department
o Members of staff within other operational Business Units o Members of staff within Support Functions
Occasional Communication with:
o Members of staff and peers within Other Catalent Business Units o Staff within other parts of Catalent
o Clients and potential clients of Catalent
o Equipment vendors
Research Scientist II
2012 2015 Viracor IBT Laboratories
CLIA/CAP certified clinical laboratory
Plan, organize, coordinate and conduct simultaneously multiple projects
Assists on the technical writing and review process of validation protocols and reports
Trains and instructs other scientists
Proficient at protein purification and analysis
o SDS PAGE gel electrophoresis (1 dimensional)
o Western Blotting used in clinical trials to analyze the presence of shed proteins in cancer vaccinated patient samples
o Quantification using spectrophotometry
o ELISA
o ELISPOT analysis used to monitor cellular immune responses in vaccine treated patients
Proficient in cell culture technique
o Maintaining sterility of cells lines
o Optimization and validation of neutralizing antibody (Nab) assay o Ficoll isolations of PMBCs from whole blood
o Cell counting by hemocytometer, Guava PCA and Cellometer Research Associate
2012 2012 University of Kansas Medical Center
Proficient at protein purification and analysis
o Gel electrophoresis
o Western Blotting
Proficient in cell culture technique
o Maintaining sterility of cells lines
o Ficoll isolations of cancer cell lines and whole blood o Cell counting by hemocytometer
Animal Studies
o Mouse dissections
o Tail vein and intraperitoneal injections
o Oral gavage
o Ear tagging
o Mouse breeding and colony maintenance
Laboratory experience
o Equipment maintenance and calibrations
o Maintains laboratory notebook
o Writes and follows scientific protocols (SOP)
Research Associate – Laboratory Manager
2002 2015 (part time 2012 2015) University of Missouri – Kansas City
Skilled at microscopy techniques
o Laser capture microscopy
o Bright field microscopy
o Fluorescence microscopy
Proficient at protein purification and analysis
o SDS PAGE gel electrophoresis – 1 and 2 dimensional o Protein separation using isoelectric focusing
o Western Blotting
o Quantification using spectrophotometry
o ELISA
o Use of the BioCAD system for perfusion chromatography from Applied Biosystems for protein purification o Column (gravity) chromatography – ion exchange liquid chromatography and affinity liquid chromatography; Qiagen Spin columns
Experienced at DNA and RNA purification and analysis o Gel electrophoresis
o Southern and Northern blotting
o Polymerase chain reaction (PCR) used for genotyping mice o Reverse transcriptase polymerase chain reaction (RT PCR) o Quantification using spectrophotometry
o Rapid amplification of cDNA ends (RACE)
o RNA interference in cell culture used for protease identification required for primary bone mineralization o Genotyping by mouse tail DNA extraction for gene knock out identification o qPCR UMR cell extracts and extracted mouse tissues
Proficient in cell culture technique
o Maintaining sterility of cells lines
o Radiolabeling proteins in cell culture
o Cell counting by hemocytometer
Animal Studies
o Mouse dissections
o Ear tagging
o Mouse breeding and colony maintenance
Laboratory Management
o Train and supervise students in Summer Scholar program o Train and supervise new laboratory assistants, post docs and visiting scholars o Maintain and order inventory of reagents and consumables o Assist in writing publications and production of figures and posters for meetings o Authorship on 8 scientific journal articles and 8 abstracts for poster presentations Research Technician
2000 2002 Clinical Reference Laboratory
Experienced at heavy metal analysis on blood and urine samples using atomic adsorption spectrophotometry
Proficient at protein analysis
o ELISA
Laboratory experience
o Equipment maintenance and calibrations
o Maintains laboratory notebook
o Writes and follows scientific protocols (SOP)
COMPUTER SKILLS
Microsoft Word
Excel
Outlook
PowerPoint
Adobe Photoshop
SigmaStat and SigmaPlot
GraphPad
AccentLab and LIMS
Molecular Devices SoftMax Pro
EDUCATION
UNIVERSITY OF MISSOURI – KANSAS CITY – Kansas City, MO Master of Science in Molecular and Cellular Biology, 2006 (GPA 3.697) Distinguished Master’s Thesis award, Graduate Research Assistantship recipient, Chi Omega Award of Excellence, Martha E. Mitchell Award and Elizabeth Lillard Award
KANSAS STATE UNIVERSITY – Manhattan, KS
Bachelor of Science in Biology, 1999 (GPA 3.438)
KANSAS CITY KANSAS COMMUNITY COLLEGE – Kansas City, KS Associate of Science, 1997 (GPA 3.96)
Presidential Honor’s Scholarship recipient, Dean’s Honor Roll, Mu Delta Chapter of Phi Theta Kappa Honor Society PUBLICATIONS & PRESENTATIONS
Huffman NT, Lovitch D, Artiques A, Gorski JP. Proteomic analysis of bone biomineralization foci isolated by laser capture microscopy. The FASEB Journal. 2004 May;18(8):C29. (Abstract) Huffman NT, Lovitch D, Midura RJ, Gorski JP. Mineral nucleation within biomineralization foci requires serine protease activation of bone acidic glycoprotein 75. J Bone Miner Res. 2005 Sept;20(Suppl.1):S60. (Abstract) Gorski JP, Huffman NT, Wright H. Proteomic analysis of bone biomineralization foci isolated by laser capture microscopy from osteoblast cultures. J Bone Miner Res. 2005 Sept;20(Suppl.1):S109. (Abstract) Huffman NT, Keightley JA, Chaoying C, Midura RJ, Lovitch D, Veno PA, Dallas SL,Gorski JP. Association of specific proteolytic processing of bone sialoprotein and bone acidic glycoprotein 75 with mineralization within biomineralization foci. J Biol Chem. 2007 Sep 7;282(36):26002 13.
Gorski JP, Huffman NT, Henderson E, Cui S, Midura RJ. Nucleation of mineral crystals within biomineralization foci requires serine protease SKI 1 (site 1). 9th ICCMBT. 2007. Nov; p3. (Abstract) Wang C, Wang Y, Huffman NT, Gorski JP. Confocal laser Raman microscopic analysis of individual biomineralization foci reveals changes in protein signals preceding mineral nucleation 9th ICCMBT. 2007. Nov; p62. (Abstract) Gorski JP, Huffman NT, Chittur SV, Liu S, Quarles LD, Midura RJ, Seidah NG. Serine protease inhibitor AEBSF blocks transcription of phex by osteoblasts. J Bone Miner Res. 2008 Sept;23(Suppl.1):S386. (Abstract) Gorski JP, Huffman NT, Xiao Z, Liu S, Studer D, Rowe PSN, Quarles LD. Phex binds to SNARE associated protein snapin and co localizes in vesicle enriched, extracellular matrix micro environment mediating mineral crystal nucleation. J Bone Miner Res. 2008 Sept;23(Suppl.1):S91. (Abstract)
Huffman NT, Chaoying C, Chittur SV, Oxford JT, Keightley JA, Midura RJ, Gorski JP. Enrichment of type XI collagen and 6b N terminal domain at sites of mineral nucleation within osteoblastic cultures. J Bone Miner Res. 2008 Sept;23(Suppl.1):S148.
(Abstract)
Gorski JP, Huffman NT, Cui C, Henderson EP, Midura RJ, Seidah NG. Potential role of proprotein convertase SKI 1 in the mineralization of primary bone. Cells Tissues Organs. 2009;189(1 4):25 32. Wang C, Wang Y, Huffman NT, Cui C, Yao X, Midura S, Midura RJ, Gorski JP. Confocal laser Raman microspectroscopy of biomineralization foci in UMR 106 osteoblastic cultures reveals temporally synchronized protein changes preceding and accompanying mineral crystal deposition. J Biol Chem. 2009 Mar 13;284(11):7100 13. Gorski JP, Huffman NT, Chittur S, Midura RJ, Black C, Oxford J, Seidah NG. Inhibition of proprotein convertase SKI 1 blocks transcription of key extracellular matrix genes regulating osteoblastic mineralization. J Biol Chem. 2011 Jan 21;286(3):1836 49. Studer D, Hillmann Marti T, Huffman NT, Gorski JP. Eliminating exposure to aqueous solvents is necessary for the early detection and ultrastructural elemental analysis of sites of calcium and phosphorus enrichment in mineralizing UMR106 01 osteoblastic cultures. Cells Tissues Organs. 2011;194(2 4):138 45. McGuire JD, Mousa AA, Zhang BJ, Todoki LS, Huffman NT, Chandrababu KB, Moradian Oldak J, Keightley A, Wang Y, Walker MP, Gorski JP. Extracts of irradiated mature human tooth crowns contain MMP 20 protein and activity. J Dent. 2014 May;42(5):626 35.
Achilleos A, Huffman NT, Marcinkiewicyz E, Seidah NG, Chen Q, Dallas SL, Trainor PA, Gorski JP. MBTPS1/SKI 1/S1P proprotein convertase is required for ECM signaling and axial elongation during somitogenesis and vertebral development. Hum Mol Genet. 2015 May 15;24(10):2884 98.
Gorski JP, Huffman NT, Vallejo J, Brotto L, Chittur SV, Breggia A, Stern A, Huang J, Mo C, Seidah NG, Bonewald L, Brotto M. Deletion of Mbtps1 (Pcsk8, S1p, Ski 1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age. J Biol Chem. 2016 Feb 26;291(9):4308 22.
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