Chemist & Scientist

Katarzyna Munson

Upland, CA ***** • 909-***-****

***************@*****.*** • w ww.linkedin.com/in/katarzynamunson Scientist & Chemist

Accomplished Scientist and Chemist with over 20 years experience and acute knowledge of principle chemical/pharmaceutical technology and analytical processes and concepts. Proved instrumental in designing, developing, conducting and troubleshooting challenging research problems. Possesses over 10 years experience in analytical chemistry and organic synthesis (HPLC, Spectroscopy, Chromatography), over 5 years experience in molecular biology (PCR, qPCR, cloning, RNA and DNA isolations, Mini-Preps) as well as in cell culture (Cell Line Passage, Trypan Blue counts). Equipped with the ability to handle HPLC (Waters and Shimadzu) with Millennium and LabSolution software. Skilled in collecting samples for tests, checking if substances meet standards, preparing reports, operating specialized equipment, and ensuring safety at the workplace. Areas of Expertise:

● Multi-Step Organic Synthesis

● Health & Safety Compliance

● Separation & Purification

● Trained in Lab Safety

● Qualitative & Quantitative Analysis

● Technical Monitoring & Reporting

● Tuning & Calibration

● Lab Equipment & Instruments

● Stability Samples Analysis

● Sampling & Analysis Planning

● Staff Training & Development

● Cross-Functional Leadership

Professional Experience

AIE PHARMACEUTICALS, Ontario, CA

Quality Assurance Chemist, ( 2018)

Performed all quality management activities, worked on Shimadzu LC with Lab-Solution software, organized daily workflow, and maintained excellent record of batch files. Prepared experimental formulas for testing and evaluation and released final product and created Certificates of Analysis.

● Improved analytical methods and procedures to streamline efforts and reduce preparation time.

● Developed and revised SOPs and standards used for conducting analysis on Perkin Elmer Spectrum Two FT-IR Spectrometer.

● Created method and performed testing on Perkin Elmer Spectrum Two FT-IR Spectrometer. BECKMAN COULTER INC., Brea, CA

Development Scientist, ( 2016 – 2017)

Delivered highly analytical support in the development of a molecular diagnostics assay for detection of Trichomonas vaginalis on the DxN Veris System. Performed calibration, maintenance and routine sample analysis and maintained all instruments and equipment.

● Commended by senior scientist and achieved Outstanding General Laboratory Practices Award- March 2017.

● Designed, executed, analyzed and documented pre-verification studies, devised verification protocols and generated reports.

● Calibrated lab equipment and prepared samples in a BSL2 environment and handled clinical samples to achieve results. CITY OF HOPE, UROLOGY SURGERY, Duarte, CA

Staff Scientist, ( 2011 – 2016)

Executed qPCR to d etect specific DNA sequences in clinical samples for biopsy and expressed Prostatic Secretion (EPS) specimens for five cDNA cancer biomarkers. Maintained PC3, HK293 cells in culture.

● Cloned Fragile Histidine Triad (FHIT) gene for DNA methylation studies; reviewed results for accuracy and resolved issues. Katarzyna Munson ***************@*****.*** • 909-***-****

● Extracted and purified M.EcoRII and Human DNMT1 for DNA methylation studies and documented all testing reports. CITY OF HOPE, UROLOGY SURGERY, Duarte, CA

Postdoctoral Fellow (2004 – 2011)

Synthesized and purified GuaUre-dR nucleoside and its oligomers (using HPLC, PAGE) for inhibition of DNA methylation and DNA structural studies.

● Rendered key scientific knowledge and lab experience to synthesize and purify (using HPLC, PAGE) Taqman probes

(FAM-TAMRA, Hex-BHQ-1 Cy5-BHQ-3) for DNA methylation studies. BIOFARM INC., Poznan, Poland

Staff Scientist, ( 2004)

Conducted stability and quantification studies of Zolpidem and Clopidogrel using HPLC and UV-Vis. Analyzed data, identified problems and performed root cause analysis by different suitable methods.

● Participated in method validation, instrument qualification and stability study accomplished in 2-day course. Scientific Research & Technical Skills

Nucleic Acids Chemistry: Phosphoramidates synthesis and purification, DNA synthesis, purification of oligomers: HPLC-Waters System (PRP-1, Nova Pak C18, XTerra RP18), PAGE

Molecular Biology: qPCR, Primer/Probe designing (Taqman), cloning, plasmid extraction, RNA isolation, cDNA Synthesis, DNA/RNA extraction, agarose gel, Agilent microfluidics electrophoresis, DNA methylation assay

(GF/C-filter 3H-SAM Activity Assay), PAGE, tissue culture Protein chemistry: Extraction, Purification: ÄKTA protein purifier system (Hi-Trap DEAE-FF, Hi-Trap SP FF), P-Cellulose, DEAE Sepharose, Hydroxyapatite (HAP) column, SDS-PAGE HPLC: Waters (Millenium Software) and Shimadzu (LabSolution Software) Spectroscopy: UV-Vis Spectrophotometer, FT-IR Spectrometer Chromatography: TLC, Silica gel column, HPLC

DNA Synthesizers: Cyclone Plus DNA Synthesizers, Expedite DNA Synthesizers Publications

● K. Lamparska and S.S. Smith, (2015), The Genetic and Epigenetic Effects of 5-Azacytidine and its Major Breakdown Product Guanylurea. Epigenetic Diagnosis & Therapy, (1) 2 8 - 36.

● K. Lamparska, J. Clark, G. Babilonia, V. Bedell, W. Yip and S.S. Smith, (2012), 2'-Deoxyriboguanylurea, the primary breakdown product of 5-aza-2'-deoxyribocytidine, is a mutagen, epimutagen, inhibitor of DNA methyltransferases and inducer of 5-azacytidine-type fragile sites. Nucleic Acids Research, 40 ( 19) 9788 - 801.

● J.P. Clark, K.W. Munson, J.W. Gu, K . Lamparska-Kupsik, K .G. Chan, J.S. Yoshida, M.H. Kawachi, L.E. Crocitto, T.G. Wilson, Z. Feng, and S .S. Smith, (2008), Performance of a Single Assay for both Type III-Type VI TMPRSS2:ERG fusions in Non-Invasive Prediction of Prostate Biopsy Outcome. Clinical Chemistry. (54) 2007-2017.

● K. Munson, J. Clark, K. L amparska-Kupsik, S .S. Smith, (2007), Recovery of bisulfite-converted genomic sequences in the methylation-sensitive QPCR. Nucleic Acids Res., (35) 2893 – 2903.

● E. Singer, K . Lamparska-Kupsik, J. Clark, K.Munson, L. Kretzner, and S.S. Smith, (2006), Nucleoprotein-Based Nanodevices in Drug Design and Delivery. Nanotechnology in Biology a nd Medicine Methods, Devices and Applications Ed. by T. Vo-Dinh, CRC Press, LLC 73 - 87.

● G. Wenska, K. Taras-Goslinska, K. Lamparska-Kupsik, B. Skalski, M. Gdaniec and Z. Gdaniec, (2002), Photochemical transformations of 5-halogeno-4-thiouridines. J.Chem.Soc.Perkin.Trans. (1) 53 - 57. Education & Credentials

Ph.D. /M.S. in Chemistry Adam Mickiewicz University, Poznan, Poland Merit scholarship of the Polish Ministry of Education Academic merit scholarships of the Adam Mickiewicz University

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