Research Safety Officer

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SHERWIN A. JACK

***** ** *** ** *** ***, Pembroke Pines, FL 33025

334-***-**** (Mobile) **************@*****.*** CANCER BIOLOGIST

MICROBIOLOGY ANIMAL TESTING CANCER BIOLOGY & RESEARCH VIROLOGY TRANSLATIONAL RESEARCH BIOCHEMISTRY DRUG TESTING MOLECULAR & CELL BIOLOGY GENETICS MULTIDISCIPLINARY RESEARCH BIOTECHNOLOGY CHEMISTRY TOXICOLOGY PHARMACOLOGY INTERDISCIPLINARY RESEARCH IMMUNOLOGY Dedicated, and versatile Research Scientist with a Ph.D. in Integrative Biosciences. Has 8+ years of demonstrated scientific expertise in oncology, mastering an array of molecular and cell biological techniques. Possess in-depth knowledge of cancer therapies having performed several in vitro, ex vivo and in vivo assays towards the testing of cancer therapeutics /drugs. Worked closely with clinical oncology teams to successfully complete research projects. Equipped with outstanding communication and interpersonal skills

LABORATORY SKILLS & TECHNIQUES

Toxicity Assays Expression Cloning Animal Models Macromolecule Blotting Viability Assays HPLC Quantitative RT-PCR Cell Culture Fluorescence Microscopy DNA & RNA Isolation Protein Purification Immunohistochemistry Flow Cytometry Dose -Response Curves Gel Electrophoresis Transfections AREAS OF EXCELLENCE

Microbiology Biological Agents Oncology Therapeutics Molecular Biology Drug Testing Toxicology modelling Exposure & Risk Assessments Immunotherapies Genetic Engineering Immunology Transgenic Plants Pathology Seminar & Conference Presentations Biological Agents EDUCATION

Ph.D. in Integrative Biosciences Aug 2010 - May 2015 Tuskegee University Tuskegee, AL Department of Biology Dissertation: The Development of a Novel Therapy against Bladder Cancer GPA: 4.0 out of a possible 4.0

Master of Science degree in Biology Aug 2008 – May 2011 Tuskegee University Tuskegee, AL Department of Biology Dissertation: The Role of miR-26a during Prostate Cancer Progression GPA: 4.0 out of a possible 4.0

Bachelor of Science degree in Biology Aug 2003 – May 2007 Tuskegee University Tuskegee, AL Department of Biology GPA: 3.9 out of a possible 4.0

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EMPLOYMENT

ADJUNCT BIOLOGY PROFESSOR May 2017 – Current

Broward College Pembroke Pines, FL

Science and Wellness Department

Courses Taught:

BSC2010, Introduction to Biology BSC2010L; Introduction to Biology BSC2011, Introduction to Biology II BSC2011L; Introduction to Biology II

BIOLOGY LABORATORY COORDINATOR Aug 2007 - Aug 2008 Tuskegee University

Department of Biology Tuskegee, AL

Managed the re-vamping of two critical laboratories: (1) Genetics and (2) Molecular Cell Biology

Provided technical support regarding the selection and use of genetically engineered microorganisms, biological agents, industrial chemical reagents, and laboratory equipment

Maintained microbiology organism cultures, cell cultures, algae, fruit flies, mice

Coordinated with the College's Safety Officer regarding storage and disposal of industrial chemicals, specimens, genetically engineered microorganisms, and other biological agents

Ordered, stored, inventoried, and distributed samples, industrial chemicals, biological agents, and other materials for the Biology Department (microbiology, immunology and genetics laboratories) RESEARCH EXPERIENCE

VISITING GRADUATE SCHOLAR (Ph.D.) Dec 2012 - May 2015 Purdue University Department of Biological Sciences West Lafayette, IN With the support of the Graduate Program and my mentors I conducted the dissertation research component of my PhD degree off-site at Purdue University, while the degree itself was conferred by Tuskegee University. My thesis committee comprised of faculty from both institutions

Developed a cancer therapeutic focused on the exploitation of a modified chimeric bacterial toxin with a redirected specificity towards the Epidermal Growth Factor Receptor (EGFR) as a therapy against bladder cancer

Evaluated the efficiency of this biological toxin to eliminate bladder cancer cells in vivo and in vitro

Worked with a multidisciplinary team of veterinarian clinical oncologists in administering, optimizing, and evaluating the toxicity risk of the said therapy on potential canine patients

Developed, designed and implemented experiments and protocols to assess post-exposure toxicity of biological agents in canine and murine (mice) animal models

Extensively analyzed and interpreted toxicity data for humans, canine, and murine (mice) animal models. Presented findings and made recommendations where needed

Exhaustively reviewed and evaluated risk assessment data related to carcinogens such as industrial chemicals and biological agents (toxins) in humans, mice and canines

Attended several lectures & presentations on the cutting-edge cancer therapies, nanoparticle - based therapies and technologies hosted by Purdue Institute for Drug Discovery

Trained in the revision and evaluation of: Investigational New Drugs Applications (INDs), Biologics License Applications (BLAs); as well as Premarket Applications (PMAs) and Investigational Device Exemption (IDE) for oncology medical devices

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GRADUATE STUDENT (M.S.) Aug 2008 - May 2011

Tuskegee University Department of Biological Science Tuskegee, AL

Investigated the role of micro-RNA-26a in metastatic prostate cancer progression

Explored the expression of a nanosized biological agent (micro-RNA-26a) in immortalized and primary prostate cancer cell lines

Developed and executed experiments with malignancies of the breast and pancreas to identify and functionally characterize other micro-RNAs, proteins, and other biological agents implicated in prostate cancer progression

Constructed and utilized genetically engineered microorganisms in expression cloning and reporter assays

Evaluated, analyzed and interpreted related data. Made relevant recommendations and presented findings at professional conferences

PATHOLOGY FELLOWSHIP May - Aug 2012

University of Alabama School of Medicine

Department of Pathology (Anatomical) Birmingham, AL

Performed tissue fixation and processing, immunohistochemistry, tissue analysis and microscopy of human clinical tissue samples from patients with prostate, breast and pancreatic cancer

Performed histopathologic scoring of processed human tissue after exposure to toxins and other biological agents

Trained in, as well as, shadowed medical doctors during informed consent procedures in appropriating patient samples, and patient recruitment for clinical studies PROSTATE CANCER SCHOLAR Jun - Aug 2006

University of North Texas Health Science Center UNTHSC-HBCU Tuskegee University Tuskegee, AL

Collaborative Summer Training Program in Prostate Cancer

Conducted and presented research on the synergistic effect of Ibuprofen and Epigallocatechin Gallate (EGCG) on prostate cancer progression at professional scientific conference

Attended seminars and conferences on advances in prostate cancer treatment and therapeutics RESEARCH ASSISTANT Aug 2005 - Aug 2007

Plant Biotechnology and Genomic Laboratory

Tuskegee University Tuskegee, AL

Supported gene expression profiling to determine the physiological role of t-Zeatin riboside (ZR) in sweet potato storage root initiation and enlargement

Provided technical support in comparative gene expression profiling of the physiological role of t- Zeatin riboside (ZR) between in vitro and hydroponic-grown sweet potato during storage root initiation and enlargement

Supported development of protein-enriched genetically engineered (transgenic) sweet potato plants

Conducted hormonal (e.g. t-Zeatin) experiments, bacterial and plant DNA extraction, tissue and organ culture of genetically engineered (transgenic) plants

Constructed and utilized genetically engineered microorganisms in expression cloning and reporter assays

Acknowledged author on published abstracts, several posters presented at scientific conferences 4

PROGRAM COORDINATION

PROGRAM COORDINATOR & FACILITATOR May – Jun 2011

Pittsburgh-Tuskegee Prostate Pre-Training Program

Tuskegee University Tuskegee, AL

Organized and conducted a training workshop geared towards preparing Tuskegee University students for an upcoming summer research internship at the University of Pittsburgh

Lectured and trained students in various microbiology and molecular biology techniques TEACHING & MENTORSHIP EXPERIENCE

BIOLOGY INSTRUCTOR Aug 2007 - May 2011

Tuskegee University Tuskegee, Department of Biological Sciences Courses Taught

Biology 250/251, Molecular Cell & Genetics Biology 2008 – 2010 Biology 231, Cell & Genetics Biology 2008 – 2010

Biology 309, Genetics 2007 – 2011

Created lectures, exams, projects, and homework assignments, graded exams and quizzes

Tutored students in Genetics and established the ‘Genetics extra hours study group initiative’

Instructed students on, and constructed genetically engineered microorganisms by conferring antibiotic resistance to otherwise susceptible native bacterial strains

Amended the Genetics Laboratory Syllabus, specifically introducing modern bacterial transformation and DNA transfection techniques and technology MENTORSHIP

Purdue University Summer Research Opportunities Program (SROP) May - Jul (2013, 2014) West Lafayette, IN

Trained SROP undergraduate students in their laboratory research projects which focused on

‘nano-scale’ cancer therapeutic project

Under my mentorship, a 2013 mentee won the “most outstanding research project”

Trained mentees to analyze, interpret, and present the data obtained in their research PEER-REVIEWED PUBLICATIONS

Sherwin Jack, John Collier, Deborah Knapp, Claudio Aguilar. A Novel EGFR-targeted Therapy against Bladder Cancer. (manuscript in preparation)

The standard primary therapy against non-muscle invasive bladder cancer (NMIBC) is the transurethral resection of the bladder tumor (TURBT). Even after TURBT, NMIBC is characterized by high rates of recurrence and progression with two-thirds of patients re-visited with the disease within 5 years of the procedure. Accordingly, there is a tremendous need for the development of a more effective primary therapy against this malignancy. Here we examined the ability of the EGF-toxin to eradicate bladder cancer cells in vitro and in vivo. The toxin comprises of (1) mPAEGF- a modified protective antigen with a redirected specificity towards EGFR, and (2) LFN-DTA - chimeric protein comprising of the N-terminal of the lethal factor fused to the catalytic domain of diphtheria toxin (DTA). The EGF-toxin was both very specific and potent in eliminating 98% of T24 human bladder cancer cells in vitro after 3 min. MB49WT murine bladder cancer cells were equally sensitive to the toxin with a LC100 of 2nM; however, the low 5

EGFR-expressing MB49 variant, MB49LE bladder cancer cells, was unaffected at this concentration in vitro. Canine patients that spontaneously presented with bladder cancer were intravesically treated with the EGF- toxin and experienced a significant overall tumor volume reduction of 40% after a single round (once per day for 5 consecutive days) of treatment as measured with ultrasound. Further, no side effects or signs of discomfort were observed in any of the canine patients. As such, this novel strategy represents a promising primary therapeutic approach for bladder cancer treatment OTHER PROCEEDINGS AND PUBLISHED ABSTRACTS

Sherwin Jack, Shaniece Theodore, Timothy Turner, and Clayton Yates. The Role of miR-26a during Prostate Cancer Progression. Department of Biology and Center for Cancer Research, Tuskegee University, Tuskegee, AL. First Bioethics Conference on Cancer Health Disparities Research, January 2012, Tuskegee, AL (Abstract – Poster)

Sherwin Jack, Shaniece Theodore, Timothy Turner, and Clayton Yates. The Role of miR-26a during Prostate Cancer Progression Department of Biology and Center for Cancer Research, Tuskegee University, Tuskegee, AL. 11th Annual Biomedical Research Symposium, September 2010, Tuskegee, AL (Abstract – Poster)

Sherwin Jack, Shaniece Theodore, Timothy Turner, and Clayton Yates. The Role of miR-26a during Prostate Cancer Progression. Department of Biology and Center for Cancer Research, Tuskegee University, Tuskegee, AL. TU/MSM/UAB Summer Internship Program, August 2009, Birmingham, AL (Abstract – Poster)

Egnin, M., D. Mortley, E. F. Sanders, S. Traore, G. Gao, S. Jack, and T. Radwan. 2008. Comparative Gene Expression Profiling and the Physiological Role of t-Zeatin Riboside (ZR) Between In Vitro and Hydroponic-grown Sweet potato During Storage Root Initiation and Enlargement. In Vitro Cell and Dev. Journal 44 (4): 355-356

Egnin, M., G Gao, D. Mortley, J. Scoffield, S. Jack, G. He and B, Bey. 2007. Gene Expression Profiling and the Physiological Role of t-Zeatin Riboside (ZR) in sweet potato Storage Root Initiation and Enlargement. HortScience, 42 (4): 976

AWARDS & HONORS

Tuskegee University Tuskegee, AL

Tuskegee University Cancer Fellow (Outstanding Graduate Student) (2009 – 2010), (2010 – 2011) The Howard P. Carter, Sr. Memorial Outstanding Undergraduate Academic Achievement Award (2007) The Beatrice E. Dopwell Memorial Academic Award (2005) Distinguished Presidential Scholarship (2003 – 2007) Tuskegee University Dean’s List (2003 – 2007)

COMMUNITY VOLUNTEER INVOLVEMENT

Tuskegee Nursing Homes Student Volunteer Tuskegee, AL (2009 – 2012) Booker T. Washington High School Math & Science Tutor Tuskegee, AL (2009 – 2012)

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