James Bufan Qiao
**** ****** **, *********, ** **517
******@*****.*** / 630-***-****
Summary
Self-motivated, disciplined engineering professional with a B.S. in Chemical Engineering and significant research experience. A self-directed candidate with a strong desire to contribute in technical, R&D, and process improvement functions. Ready and willing to take up leadership/management positions. Willing to travel. Available July 2018.
Education
Washington University in St. Louis St. Louis, MO
Bachelor of Science in Chemical Engineering GPA 3.93/4.0
Summa Cum Laude
Dean’s list (All Semesters)
Technical Skills
Software: MATLAB, JAVA, Aspen HYSYS, Microsoft Office Suite
Equipment: Scanning Electron Microscopy, Gas Chromatography Mass Spectroscopy, Optical Microscopy, Fourier Transform Infrared Spectroscopy, Tensometer Testing, Large Scale Fermenter
Methods: Large Scale Cell Culturing, Genome Editing Techniques (Ligation/Transformation), PCR design, TUNEL assay, Frozen Section Processing and Analysis, Fatty Acid Extraction and Purification, Coomassie Stain Analysis
Engineering and Research Experience
Washington University in St. Louis, Department of Chemical Engineering St. Louis, MO
Chemical Engineering Capstone Spring 2017
Lead a team to design the most efficient, financially viable theoretical butanol biofuel plant out of the class.
Wrote and evaluated process flow diagrams, material and energy balances, and equipment specification sheets for the whole plant
Redesigned a traditionally batch fermentation process into an efficient continuous-batch process to maximize each fermenter’s effective usage and to ensure the flawless integration of fermentation with downstream processes.
Selected to present the project at a yearly department of chemical engineering faculty meeting.
Research Technician August 2017-May 2018
Engineered mussel foot protein genes into the E. coli genome to synthesize heterogeneous mussel foot proteins.
Enhanced material properties of graphene oxide sheets by two to three fold higher than the highest reported values through stabilizing graphene oxide sheet interactions with heterogeneous mussel foot proteins.
Undergraduate Researcher June 2014-May 2017
Introduced foreign genes, such as BKD, into E. coli to alter the native fatty acid synthesis pathway to selectively produce medium-length, branched chain fatty acids.
Designed and iterated through several upstream and downstream pathway schemes to systematically identify advantages and weaknesses.
Loyola University, Department of Ophthalmology Chicago, IL
Research Volunteer June 2013
Determined the roles of CCL2 and DAF1 in retinal health and diseases.
Performed situ apoptosis (TUNEL) assay and histology experiments in CCL2 and DAF1 single or double knockout mice.
Publications and Presentations
Yu MZ, Kang K, Bu P, Bell BA, Kaul C, Qiao JB, Sturgill-Short G, Yu XS, Tarchick M, Beight C, Zhang SX, Peachey NS. Deficiency of CC chemokine ligand 2 and decay-accelerating factor causes retinal degeneration in mice. Exp Eye Res, 2015; 138:126-133
Jiang W, Qiao JB, Bentley G, Liu D, Zhang F. Modular pathway engineering for the microbial production of branched-chain fatty alcohols. Biotechnology for Biofuels, 2017; 10:244
Kim E, Qiao JB, Zhang F. Enhanced material properties of graphene oxide films using heterogenous mussel foot proteins crosslinkers. In preparation.
Jiang W, Qiao JB, Zhang F. Genetic Engineering of Escherichia coli to produce bi-functional molecules. Poster session at the Undergraduate Research Symposium hosted by Washington University Office of Undergraduate Research, 2016.
Extracurricular Experience
Naperville North High School, Summer Cross Country Camp Naperville, IL
Volunteer Assistant Coach June 2017- August 2017, June 2018 – Present
Taught the high school students best practices learned from collegiate running to not only improve in running, but also in general health and lifestyle.
Initiated dialogue on maintaining proper hydration through hot summer months, prompting the campers to develop preventative hydration habits.
Washington University in St. Louis St. Louis, MO
Engineering Student Services Tutor August 2015-May 2017
Tutored in General Chemistry, Differential Equations, Organic Chemistry, Thermodynamics, Analysis of Chemical Systems, Engineering Mathematics, and General Physics.
Boosted exam and class grades by at least a whole letter grade by teaching students more efficient methods of studying and test preparation.
St. Peter and Paul, Biddle House St. Louis, MO
Volunteer December 2017-May 2018
Formed meaningful bonds with the shelter residents and motivated them to transition back into a working society.
Barnes Jewish Hospital, Post Pulmonary Care Unit St. Louis, MO
Volunteer June 2016-May 2018
Held impromptu ukulele concerts for the patient under extremely restrictive and isolating conditions.
Washington University in St. Louis St. Louis, MO
Cross Country, Track and Field member August 2013-May 2017
Organized mandatory morning summer runs for teammates in the St. Louis area to motivate consistent summer training and to promote team bonding.
Participated in the 2015 and 2016 University Athletics Association NCAA DIII Indoor Track and Cross Country Conference Meets.